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How to evaluate a recent structure?
Solution Structure of Enzyme IIAChitobiose from theN,N’-Diacetylchitobiose Branch of the Escherichia coli Phosphotransferase System
Chun Tang, David C. Williams, Jr., Rodolfo Ghirlando, and G. Marius Clore
J.Biol.Chem . 280, 11770–11780, (2005)
How to evaluate a structure?
The Ramachadran Plot & ProCheck NMR
The Ramachadran Plot & ProCheck NMR
The Ramachadran Plot & ProCheck NMR
New Long Range Distance Constraints - the RDC
RDC = residual dipolar coupling
New Long Range Distance Constraints - the RDC
New Methods - TROSY
TROSY = Transverse Relaxation Optimized SpectroscopY
New Methods -TROSY
New Methods - deuteration of proteins [1H -> 2H]
New Methods - 2H
Large Proteins - specific methyl labeling, 2H, TROSY, 4D NMR
Large Proteins - specific methyl labeling, 2H, TROSY, 4D NMR
Large Proteins - specific methyl labeling, 2H, TROSY, 4D NMR
Large Proteins - specific methyl labeling, 2H, TROSY, 4D NMR
Large Proteins - specific methyl labeling, 2H, TROSY, 4D NMR
Large Proteins - specific methyl labeling, 2H, TROSY, 4D NMR,CH3 <-> NH NOE’s
All protons only NH’s NH’s and some CH3’s
back exchanged into 2H protein selective labeling
Hard proteins like membrane proteins - Phospholamban
Phospholamban
Phospholamban
Phospholamban
