4
Issue 7 CYANOnews is the Newsleer of CYANOCOST (ES 1105 COST Acon) It is published bimonthly and it contains news on cyanobacteria & cyanotoxins and from the CYA- NOCOST network such as: Acvies and outcomes Conferences, workshops Jobs, scholarships Publicaons News Research projects US EPA Efforts to address HABs Impacng Drinking & Recreaonal Waters 1 ICHA2018 Sessions an- nounced 2 GlobalHAB new website 2 CYANOjobs 3 CYANOevents 3 CYANOresearch: Maria Antoniou 4 CYANOpapers 4 Inside this issue: US Environmental Protecon Agency Efforts to address Harmful Algal Blooms (HABs) Impacng Drinking and Recreaonal Waters Mar-Apr 2018 Lesley D’Anglada, U.S. EPA Office of Water/Office of Science and Technolo- gy [email protected] Nicholas Dugan, U.S. EPA Office of Research and Development/Naonal Risk Management Research Laboratory [email protected] Blake Schaeffer, U.S. EPA Office of Research and Development/Naonal Exposure Research Laboratory schaeff[email protected] James Lazorchak, U.S. EPA Office of Research and Development/Naonal Exposure Research Laboratory [email protected] The US Environmental Protecon Agency (EPA) is taking several acons to address problems posed by harmful algal blooms (HABs) in surface and drinking waters, including conducng monitoring in drinking and recreaonal waters and the devel- opment of health risk assessments. Under the Unregulated Contaminant Monitoring Rule, the EPA is iniang a two-year effort to monitor for ten cyanotoxins analytes in the source and fin- ished waters of public water systems. The EPA also included cyanobacteria and some cyanotoxins in the 2007 and 2012 Naonal Lakes Assess- ment survey. Other future surveys, such as the wetland, rivers and stream, and coastal condi- on assessments will also monitor for cyanotoxins. In 2015, the EPA developed non-regulatory health advisories for microcysns and cylindrospermopsin, developed Health Risk Assessments for microcysns, cylindrospermopsins, and anatoxin-a, and published a variety of support docu- ments to assist public water systems. In addion, in 2016, EPA released draſt Recreaonal Crite- ria/Swimming Advisories for microcysns and cylindrospermopsin, and in 2017 released sup- port documents for managers of recreaonal water bodies. The EPA’s research program includes efforts in the areas of monitoring, analycal method de- velopment, health effects, remote sensing, water treatment and ecosystem impacts. Results from this program include: analycal methods for several toxins in surface and finished drinking waters, a mul-year study that integrates the analysis of watershed nutrient management prac- ces with bloom monitoring in the receiving reservoir, a satellite remote sensing program com- bined with tools to make the data easily accessible, a study to evaluate the impact of cylin- drospermopsins on mammalian models, and a study to evaluate the impact of water treatment oxidants on toxin release from cyanobacterial cells. The EPA is also studying the increasing oc- currence of the marine invasive organism Prymnesium parvum (golden algae) in freshwater systems. The EPA is developing new, or refining exisng chemical, instrument, biological meth- ods and image analysis applicaons for the detecon of HABs and their toxins. These efforts include the detecon of toxins in fish ssues, molecular detecon methods (eDNA, PCR, qPCR) of HAB species and the genes responsible for toxin producon, online toxicity monitors (OTM), in vitro methods for detecon of toxicity, and flow cytometric approaches for the idenficaon of phytoplankton and zooplankton. Ecotoxicology research is also being conducted using a novel approach of tesng toxins produced by laboratory cultures.

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Page 1: Issue 7 Mar Apr 2018 US Environmental Protection Agency ... · 4/15/2018 · harmful algal looms (HA s). It is aimed at fostering and promoting o-operative resear h direted to-ward

Issue 7

CYANOnews is the Newsletter of CYANOCOST

(ES 1105 COST Action)

It is published bimonthly and it contains news on cyanobacteria & cyanotoxins and from the CYA-NOCOST network such as:

Activities and outcomes

Conferences, workshops

Jobs, scholarships

Publications

News

Research projects

US EPA Efforts to address HABs Impacting Drinking & Recreational Waters

1

ICHA2018 Sessions an-nounced

2

GlobalHAB new website 2

CYANOjobs 3

CYANOevents 3

CYANOresearch: Maria Antoniou

4

CYANOpapers 4

Inside this issue:

US Environmental Protection Agency Efforts to address Harmful Algal Blooms (HABs) Impacting Drinking and Recreational Waters

Mar-Apr 2018

Lesley D’Anglada, U.S. EPA Office of Water/Office of Science and Technolo-gy [email protected] Nicholas Dugan, U.S. EPA Office of Research and Development/National Risk Management Research Laboratory [email protected] Blake Schaeffer, U.S. EPA Office of Research and Development/National Exposure Research Laboratory [email protected] James Lazorchak, U.S. EPA Office of Research and Development/National Exposure Research Laboratory [email protected] The US Environmental Protection Agency (EPA) is taking several actions to address problems posed by harmful algal blooms (HABs) in surface and drinking waters, including conducting monitoring in drinking and recreational waters and the devel-opment of health risk assessments. Under the Unregulated Contaminant Monitoring Rule, the EPA is initiating a two-year effort to monitor for ten cyanotoxins analytes in the source and fin-ished waters of public water systems. The EPA also included cyanobacteria and some cyanotoxins in the 2007 and 2012 National Lakes Assess-ment survey. Other future surveys, such as the wetland, rivers and stream, and coastal condi-tion assessments will also monitor for cyanotoxins. In 2015, the EPA developed non-regulatory health advisories for microcystins and cylindrospermopsin, developed Health Risk Assessments for microcystins, cylindrospermopsins, and anatoxin-a, and published a variety of support docu-ments to assist public water systems. In addition, in 2016, EPA released draft Recreational Crite-ria/Swimming Advisories for microcystins and cylindrospermopsin, and in 2017 released sup-port documents for managers of recreational water bodies.

The EPA’s research program includes efforts in the areas of monitoring, analytical method de-

velopment, health effects, remote sensing, water treatment and ecosystem impacts. Results

from this program include: analytical methods for several toxins in surface and finished drinking

waters, a multi-year study that integrates the analysis of watershed nutrient management prac-

tices with bloom monitoring in the receiving reservoir, a satellite remote sensing program com-

bined with tools to make the data easily accessible, a study to evaluate the impact of cylin-

drospermopsins on mammalian models, and a study to evaluate the impact of water treatment

oxidants on toxin release from cyanobacterial cells. The EPA is also studying the increasing oc-

currence of the marine invasive organism Prymnesium parvum (golden algae) in freshwater

systems. The EPA is developing new, or refining existing chemical, instrument, biological meth-

ods and image analysis applications for the detection of HABs and their toxins. These efforts

include the detection of toxins in fish tissues, molecular detection methods (eDNA, PCR, qPCR)

of HAB species and the genes responsible for toxin production, online toxicity monitors (OTM),

in vitro methods for detection of toxicity, and flow cytometric approaches for the identification

of phytoplankton and zooplankton. Ecotoxicology research is also being conducted using a novel

approach of testing toxins produced by laboratory cultures.

Page 2: Issue 7 Mar Apr 2018 US Environmental Protection Agency ... · 4/15/2018 · harmful algal looms (HA s). It is aimed at fostering and promoting o-operative resear h direted to-ward

cently announced; they cover all as-pects of marine and freshwater HABs.

There are many sessions co-Chaired by CYANOCOST members and we ex-

The 18th edition of the International Conference on Harmful Algae (ICHA2018) will take place on 21-26 October 2018 in Nantes, France. The session topics of ICHA2018 were re-

The European Commission has is-sued a proposal for a Directive on the Quality of Water Intended for Human Consumption. The proposal is a recast of Directive 98/83/EC, which was amended in 2003, 2009 and 2015.

Microcystin-LR is included in the proposal as a “Chemical Parame-ter”, with a parametric value of 1μg/L, along with other organic compounds that are added in the list (Bisphenol A, Haloacetic acids, Nonylphenol, PFAS). Performance criteria for determination of MC-LR are specified as 30% uncertainty at the parametric value, while meth-ods should comply to the require-ments of ISO 17025.

ICHA2018: Sessions announced (Nantes,October 2018)

Microcystin-LR is included in the proposed revision of the Drinking Water Directive ! Submit your feedback.

PAGE 2/4 CYANONEW S ISSUE 7

Inclusion of MC-LR in the proposed Directive is a result of increased oc-currence of microcystins in European waters and beyond, as well as of increased awareness of the health risks and hazards associated with toxic cyanobacteria. CYANOCOST has significant contributions in this field, including two books on chemical analysis of cyanotoxins and molecu-lar detection of toxigenic cyanobac-teria and many joint papers on cy-anotoxin research. Since 2012, CYA-NOCOST has also contributed in rais-ing awareness of cyanotoxins in Eu-rope; in this sense, the proposed Directive is rewarding of the vast amount of voluntarily work done within the Network and highlights the important societal impact of COST Actions.

The proposed Directive is under public con-sultation till 2 April 2018. CYANOCOST is preparing to submit comments to support the addition of more MC congeners in the Directive. You are encouraged to submit comments through your national authori-ties or directly through the EU-website.

You can download the related documents and submit your feedback here.

Microcystin –LR (source: Chemspider)

The new GlobalHAB website is launched !

From the website:

“The Global Harmful Algal Blooms (GlobalHAB) Programme is an inter-national scientific programme on harmful algal blooms (HABs). It is aimed at fostering and promoting co-operative research directed to-ward improving the understanding and prediction of HAB events, and providing scientific knowledge to

manage and mitigate their impacts against the back-ground of global changes in climate, and increased anthropogenic pressures on aquatic ecosystems. GlobalHAB will address the scientific and societal challenges of HABs through the application of ad-vanced and cost-effective technologies, training and capacity building, with a multidisciplinary approach. It will also build linkages with broader science do-mains (climatology, toxicology, economy, medicine, public health), emphasize social science communica-tions and address management priorities. GlobalHAB will operate for 10 years from 2016 to 2025.”

GlobalHAB new website launched

pect a wide participation by CYANO-COST.

The deadline for abstract submission

is 15 April 2018.

Page 3: Issue 7 Mar Apr 2018 US Environmental Protection Agency ... · 4/15/2018 · harmful algal looms (HA s). It is aimed at fostering and promoting o-operative resear h direted to-ward

TRETILE POJECT: Development of biological processes for textile wastewaters (with valorization of cyanobacteria biomass). Politecnico di Milano, Italy.

PhD position: Bioactive compounds from cyanobacteria affecting vertebrate development, Masaryk University, Czech Re-public.

Early stage researchers in the Innovative Training Network “Solar Energy to Biomass – Optimisation of light energy conver-sion in plants and microalgae”, Position 1, Position 2, VU University, Amsterdam.

Genetic engineering in microalgae for the production of high value products, Università degli Studi di Verona, Italy.

Two Postdoctoral positions in Algal Biochemistry/Physiology and in Algal Molecular Biology, STU-UNIVPM Joint Algal Re-searcher Center, Italy.

CYANOevents

CYANOjobs

COST Info Day Greece, at NCSR Demokritos, Athens, 27 March 2018 (with CYANOCOST presentation by Theodoros Triantis).

SETAC Europe 28th Annual Meeting, Rome, Italy, 13-17 May 2018, including session 4.07 on HABs.

XXII International Mass Spectrometry Conference IMSC2018, Florence, August 26-31 2018.

The 18th International Conference on Harmful Algae (ICHA18), 21-26 October 2018, Nantes, France.

PAGE 3/4 CYANONEW S ISSUE 7

Subscribe to the US EPA—Freshwater HABs News-letter, edited by Dr. Lesley V. D’Anglada for more info on upcoming events in USA and beyond.

Presentations of the 6th International Symposium on Ma-rine and Freshwater Toxins Analysis, organized by Prof. Ana Gago-Matinez (U. Vigo) in Baiona, Spain (22-25 Octo-ber) are available on-line (streaming video + slides) by Campus do Mar. Link to presentations

Info Day Greece, NCSR Demokritos, 27 March 2018

Page 4: Issue 7 Mar Apr 2018 US Environmental Protection Agency ... · 4/15/2018 · harmful algal looms (HA s). It is aimed at fostering and promoting o-operative resear h direted to-ward

CYANOresearch

Jeremy T. Walls, Kevin H. Wyatt, Jason C. Doll, Eric M. Rubenstein, Allison R. Rober (2017). “Hot and toxic: Temperature regulates microcystin release from cyanobacteria”, Science of The Total Environment 610–611, pp. 786-795. https://doi.org/10.1016/j.scitotenv.2017.08.149.

Oddvar Myhre, Dag Marcus Eide, Synne Kleiven, Hans Christian Utkilen & Tim Hofer (2018), Repeated five-day administration of L-BMAA, microcystin-LR, or as mixture, in adult C57BL/6 mice - lack of adverse cognitive effects. Scientific Reports 8, Arti-cle number: 2308. doi:10.1038/s41598-018-20327-y

Nathaniel W. May, Nicole E. Olson, Mark Panas, Jessica L. Axson, Peter S. Tirella, Rachel M. Kirpes, Rebecca L. Craig, Matthew J. Gunsch, Swarup China, Alexander Laskin, Andrew P. Ault, and Kerri A. Pratt (2018). Aerosol Emissions from Great Lakes Harmful Algal Blooms. Environmental Science & Technology 52 (2), 397-405 DOI: 10.1021/acs.est.7b03609

Nunn, P. B. and Codd, G. A. (2017). “Metabolic solutions to the biosynthesis of some diaminomonocarboxylic acids in nature: Formation in cyanobacteria of the neurotoxins 3-N-methyl-2,3-diaminopropanoic acid (BMAA) and 2,4-diaminobutanoic acid (2,4-DAB)”. Phytochemistry 144, 253-270. https://doi.org/10.1016/j.phytochem.2017.09.015

Spoof, L., & Catherine, A. (2016). Appendix 3: Tables of Microcystins and Nodularins. Handbook of Cyanobacterial Monitoring

and Cyanotoxin Analysis (pp. 526-537): John Wiley & Sons, Ltd. http://dx.doi.org/10.1002/9781119068761.app3

CYANOpapers This column features papers that acknowledge CYANOCOST and other papers. A list of all publications can be downloaded from www.cyanocost.net (publications page).

www.cyanocost.net

You are invited to distribute CYANOnews to anyone who may be interested. You can subscribe/unsubscribe by using the Contact form in the webpage.

Send your contributions to CYANOnews, or posts for the website, facebook and twitter by e-mail to Tri Kaloudis.

Send your papers that acknowledge CYANOCOST by e-mail to Tri Kaloudis. You can acknowledge CYANO-COST in the future if you think that the network had a posi-tive contribution to your work. CYANOCOST papers will be disseminated through all CYANOCOST media.

PAGE 4/4 CYANONEW S ISSUE 7

Maria G. Antoniou (Cyprus) is an Assistant Professor at the Department of Environmental Science and

Technology of the Cyprus University of Technology. In 2012 she established the Water Treatment Labora-

tory-AQUA research group at CUT which focuses on the application of advanced oxidation treatment

processes (AOPs) for the removal of both xenobiotics and naturally produced toxic compounds from wa-

ter, wastewater, and soil. WTL-AQUA is currently the only research group in Cyprus working on projects

related to cyanobacteria and cyanotoxins, focusing on monitoring,

detection, and treatment (at source and in water treatment

plants). Maria also served as the MS for Cyprus during CYANOCOST

and has actively participate in an array of events prepared by this

action including workshops, training schools, and STSMs. This

resulted in the formation of strong collaborations and the ex-

change of researchers with other CYANOCOST- members including

the NCSR-Demokritos group led by Dr. Anastasia Hiskia (STSM for

Maria G. Antoniou, removal of MCs with AOPs), the IBED group of

the University of Amsterdam and Prof. Prof. Hans Matthijs and Dr.

Petra Visser (Erasmus Placement for Nikoletta Tsiarta, emerging

in-situ oxidation treatments for cyano-HABs control), and the CYA-

NOSOL group of the Robert Gordon University led by Prof. Linda

Lawton and Dr. Christine Edwards (Erasmus Placement: Iosef

Boarei and Danae Pantelide, removal of MC-LR and NOD with nov-

el-AOPs; Erasmus Placement to Cyprus of Louis Julien, effects of

cyanobacterial contaminated water on crops). Currently the group

is assisting the competent authorities of Cyprus with the monitoring and restoration of cyano-HABs con-

taminated locations. WTL-AQUA is utilizing data from the monitoring of surface waters to form correla-

tions between water treatment parameters and cyano-HABs formation in Cyprus. These trends are ex-

pected to differ from other European counties due to the island’s long periods of drought, semi-arid cli-

mate, and extensive periods of sunshine. This past December, the group received funding from the Re-

search Promotion Foundation of Cyprus through the “BILATERAL COLLABORATIONS: Cyprus and France”

call. CYANOS is a 2 year project titled “Monitoring and treatment of cyanobacterial contaminated surface

waters”. The AQUA group will closely collaborate with the ECOBIO "Ecosystems, Biodiversity and Evolu-

tion" Institute of the University of Rennes 1 and the group led by Prof. Luc Brient and Prof. Myriam Bor-

mans. The aim of this collaboration is the exchange of expertise and knowledge transfer from the French

partners to the Cypriot partners on various identification techniques (microscope and molecular methods)

and the taxonomy of cyanobacteria and other microalgae detected, while the latter ones will assist the

French partners with the application of hydrogen peroxide and other emerging oxidants currently tested

at AQUA for the at-source treatment of cyanobacterial blooms. More details all the projects currently

working on can be found at http://wtl-aqua.weebly.com/. None of the project outlined above would have

been possible without the CYANOCOST action thereofere, Maria and the WTL-AQUA team are grateful to

the CYANOCOST family for all the support and the long-lasting collaborations.