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ISOLATION OF HERBAL VITAMIN C

!

MISS SUCHADA NILKAMHANK

MISS ANOCHA PAOCHOM

A SPECIAL PROJECT SUBMITTED IN

PARTIAL FULLFILMENT

OF THE REQUIREMENT FOR

THE BACHELOR DEGREE OF SCIENCE IN

PHARMACY

FACULTY OF PHARMACY

MAHIDOL UNIVERSITY

2000

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Abstract

Isolation of herbal vitamin C

#

Suchada Nilkamhank, Anocha Paochom

Project adviser: Wandee Kritsanapun*, Leena Soonthonsuk**

*Department of Pharmacognosy, Faculty of Pharmacy, Mahidol

University

**Department of Pharmaceutical Chemistry, Faculty of Pharmacy,

Mahidol Univercity

Iodometry method was employed for determination of

vitamin C in Thai medicinal plants. The method was reliable

and validated with the percent recovery of 98.0 -105.0 %. The

precision of the method calculated as percent relative standard

deviations (%RSD) was 0.7-1.5% and the linearity (R2) was

0.9999-1.0000. The method offered good limit of detection and

limit of quantitation, which were 2.2 and 7.3 mg, respectively.

The method was used to determine vitamin C content in guava

(Psidium guajava Linn.), emblic myrobolan (Phyllanthus

embica Linn.), lemon (Citrus aurantifollia Swing.), sweet

pepper (Capsicum annuum Linn.), Garcinia schomburgkiana

Pierre., passion fruit (Passiflora laurifoia Linn.). The amount of

vitamin C found were guava 80.1, emblic myrobolan 226.0,

sweet pepper 52.8, passion fruit 39.1, lemon 10.5, Garcinia

schomburgkiana 4.6 mg /100 g, respectively. Emblic

myrobolan and guava juice, which contained highest Vitamin C,

were freeze–dried and re-analyzed. Vitamin C in both freeze–

dried samples decreased to 46.9 and 179.8 mg/100 g,

respectively. Stability of the freeze–dried samples was examined

by keeping the samples in a dessicator and the vitamin C content

was re-analyzed during the first and second month of the

storage. Results showed that after one month, vitamin C in

guava and emblic myrobolan juice were 33.8 and 172.7 mg/100

g, respectively and after two month traces of Vitamin C was

still existed when detected by TLC comparing to the standard.