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MS/MS Fragmenta-on of RTSNIKFQD Found in An-gen 85 complex A (Ag85A) (Mycolyl transferase 85A, Fibronec-n-‐binding protein A) -‐ Mycobacterium tuberculosis H37Rv 3804c
Ions Score: 17
# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 301.2105 151.1089 284.1839 142.5956 R 9
2 402.2581 201.6327 385.2316 193.1194 384.2476 192.6274 T 952.4734 476.7404 935.4469 468.2271 934.4629 467.7351 8
3 489.2902 245.1487 472.2636 236.6354 471.2796 236.1434 S 851.4258 426.2165 834.3992 417.7032 833.4152 417.2112 7
4 603.3331 302.1702 586.3065 293.6569 585.3225 293.1649 N 764.3937 382.7005 747.3672 374.1872 746.3832 373.6952 6
5 716.4172 358.7122 699.3906 350.1989 698.4066 349.7069 I 650.3508 325.6790 633.3243 317.1658 632.3402 316.6738 5
6 844.5121 422.7597 827.4856 414.2464 826.5015 413.7544 K 537.2667 269.1370 520.2402 260.6237 519.2562 260.1317 4
7 991.5805 496.2939 974.5540 487.7806 973.5700 487.2886 F 409.1718 205.0895 392.1452 196.5763 391.1612 196.0842 3
8 1119.6391 560.3232 1102.6126 551.8099 1101.6285 551.3179 Q 262.1034 131.5553 245.0768 123.0420 244.0928 122.5500 2
9 D 134.0448 67.5260 116.0342 58.5207 1
MS/MS Fragmenta-on of MSGLLDPSQ Found in An-gen 85 complex A (Ag85A) Mycobacterium tuberculosis H37Rv 3804c
# b b++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 132.0478 66.5275 M 9
2 219.0798 110.0435 201.0692 101.0382 S 816.4098 408.7085 799.3832 400.1953 798.3992 399.7032 8
3 276.1013 138.5543 258.0907 129.5490 G 729.3777 365.1925 712.3512 356.6792 711.3672 356.1872 7
4 389.1853 195.0963 371.1748 186.0910 L 672.3563 336.6818 655.3297 328.1685 654.3457 327.6765 6
5 502.2694 251.6383 484.2588 242.6330 L 559.2722 280.1397 542.2457 271.6265 541.2617 271.1345 5
6 617.2963 309.1518 599.2858 300.1465 D 446.1882 223.5977 429.1616 215.0844 428.1776 214.5924 4
7 714.3491 357.6782 696.3385 348.6729 P 331.1612 166.0842 314.1347 157.5710 313.1506 157.0790 3
8 801.3811 401.1942 783.3706 392.1889 S 234.1084 117.5579 217.0819 109.0446 216.0979 108.5526 2
9 Q 147.0764 74.0418 130.0499 65.5286 1
Ions Score: 17
MS/MS Fragmenta-on of QSGLSIVMPV Found in An-gen 85 complex B (Ag85B) Mycobacterium tuberculosis H37Rv 1886c
Ions Score: 18
MS/MS Fragmenta-on of QELDEISTNI Found in EsxB (10 kDa culture filtrate an-gen) -‐ Mycobacterium tuberculosis H37Rv 3874
Ions Score: 44
# Immon. a a* a0 b b* b0 Seq. v w w' y y* y0 #
1 101.0709 101.0709 84.0444 129.0659 112.0393 Q 10
2 102.0550 230.1135 213.0870 212.1030 258.1084 241.0819 240.0979 E 1115.5691 1114.5739 1189.6059 1172.5794 1171.5953 9
3 86.0964 343.1976 326.1710 325.1870 371.1925 354.1660 353.1819 L 1002.4851 1001.4898 1060.5633 1043.5368 1042.5527 8
4 88.0393 458.2245 441.1980 440.2140 486.2195 469.1929 468.2089 D 887.4581 886.4629 947.4792 930.4527 929.4687 7
5 102.0550 587.2671 570.2406 569.2566 615.2620 598.2355 597.2515 E 758.4155 757.4203 832.4523 815.4258 814.4417 6
6 86.0964 700.3512 683.3246 682.3406 728.3461 711.3196 710.3355 I 645.3315 658.3519 672.3675 703.4097 686.3832 685.3991 5
7 60.0444 787.3832 770.3567 769.3727 815.3781 798.3516 797.3676 S 558.2994 557.3042 590.3256 573.2991 572.3151 4
8 74.0600 888.4309 871.4044 870.4203 916.4258 899.3993 898.4153 T 457.2518 470.2722 472.2514 503.2936 486.2671 485.2831 3
9 87.0553 1002.4738 985.4473 984.4633 1030.4687 1013.4422 1012.4582 N 343.2088 342.2136 402.2459 385.2194 2
10 86.0964 1115.5579 1098.5313 1097.5473 1143.5528 1126.5263 1125.5422 I 230.1248 243.1452 257.1608 288.2030 271.1765 1
MS/MS Fragmenta-on of NIVNMLHGV Found in EsxP (puta-ve ESAT-‐6 like protein) -‐ Mycobacterium tuberculosis H37Rv 2347c
#775 Immon. a a* b b* Seq. v w w' y y* #
1 87.0553 87.0553 70.0287 115.0502 98.0237 N 9
2 86.0964 200.1394 183.1128 228.1343 211.1077 I 980.5094 993.5298 1007.5455 1038.5877 1021.5611 8
3 72.0808 299.2078 282.1812 327.2027 310.1761 V 881.4410 894.4614 925.5036 908.4771 7
4 87.0553 413.2507 396.2241 441.2456 424.2191 N 767.3981 766.4029 826.4352 809.4087 6
5 104.0528 544.2912 527.2646 572.2861 555.2595 M 636.3576 635.3624 712.3923 695.3657 5
6 86.0964 657.3752 640.3487 685.3702 668.3436 L 523.2736 522.2783 581.3518 564.3253 4
7 110.0713 794.4342 777.4076 822.4291 805.4025 H 386.2146 468.2677 451.2412 3
8 30.0338 851.4556 834.4291 879.4505 862.4240 G 331.2088 314.1823 2
9 72.0808 950.5240 933.4975 978.5189 961.4924 V 230.1248 243.1452 274.1874 257.1608 1
MS/MS Fragmenta-on of EALATLVVN Found in GroEL 1 (60 kDa chaperonin)-‐ Mycobacterium tuberculosis H37Rv 3417c
# b b++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 130.0499 65.5286 112.0393 56.5233 E 9
2 201.0870 101.0471 183.0764 92.0418 A 800.4876 400.7475 783.4611 392.2342 782.4771 391.7422 8
3 314.1710 157.5892 296.1605 148.5839 L 729.4505 365.2289 712.4240 356.7156 711.4400 356.2236 7
4 385.2082 193.1077 367.1976 184.1024 A 616.3665 308.6869 599.3399 300.1736 598.3559 299.6816 6
5 486.2558 243.6316 468.2453 234.6263 T 545.3293 273.1683 528.3028 264.6550 527.3188 264.1630 5
6 599.3399 300.1736 581.3293 291.1683 L 444.2817 222.6445 427.2551 214.1312 4
7 698.4083 349.7078 680.3978 340.7025 V 331.1976 166.1024 314.1710 157.5892 3
8 797.4767 399.2420 779.4662 390.2367 V 232.1292 116.5682 215.1026 108.0550 2
9 N 133.0608 67.0340 116.0342 58.5207 1
Ions Score: 14
MS/MS Fragmenta-on of IPTKRSETFT Found in Chaperone protein dnaK (Heat shock protein 70) -‐ Mycobacterium tuberculosis H37Rv 0350
Ions Score: 27
# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 114.0913 57.5493 I 10
2 211.1441 106.0757 P 1066.5528 533.7800 1049.5262 525.2667 1048.5422 524.7747 9
3 312.1918 156.5995 294.1812 147.5942 T 969.5000 485.2536 952.4734 476.7404 951.4894 476.2483 8
4 440.2867 220.6470 423.2602 212.1337 422.2762 211.6417 K 868.4523 434.7298 851.4258 426.2165 850.4417 425.7245 7
5 596.3879 298.6976 579.3613 290.1843 578.3773 289.6923 R 740.3573 370.6823 723.3308 362.1690 722.3468 361.6770 6
6 683.4199 342.2136 666.3933 333.7003 665.4093 333.2083 S 584.2562 292.6318 566.2457 283.6265 5
7 812.4625 406.7349 795.4359 398.2216 794.4519 397.7296 E 497.2242 249.1157 479.2136 240.1105 4
8 913.5102 457.2587 896.4836 448.7454 895.4996 448.2534 T 368.1816 184.5944 350.1710 175.5892 3
9 1060.5786 530.7929 1043.5520 522.2796 1042.5680 521.7876 F 267.1339 134.0706 249.1234 125.0653 2
10 T 120.0655 60.5364 102.0550 51.5311 1
MS/MS Fragmenta-on of ATTLPVQR Found in Heat Shock protein HspX (α-‐crystallin) -‐ Mycobacterium tuberculosis H37Rv 2031c
# Immon. a a* a0 b b* b0 Seq. v w w' y y* y0 #
1 44.0495 44.0495 72.0444 A 8
2 74.0600 145.0972 127.0866 173.0921 155.0815 T 768.4363 781.4567 783.4359 814.4781 797.4516 796.4676 7
3 74.0600 246.1448 228.1343 274.1397 256.1292 T 667.3886 680.4090 682.3883 713.4305 696.4039 695.4199 6
4 86.0964 359.2289 341.2183 387.2238 369.2132 L 554.3045 553.3093 612.3828 595.3562 5
5 70.0651 456.2817 438.2711 484.2766 466.2660 P 457.2518 456.2565 499.2987 482.2722 4
6 72.0808 555.3501 537.3395 583.3450 565.3344 V 358.1833 371.2037 402.2459 385.2194 3
7 101.0709 683.4087 666.3821 665.3981 711.4036 694.3770 693.3930 Q 230.1248 229.1295 303.1775 286.1510 2
8 129.1135 R 74.0237 73.0284 175.1190 158.0924 1
MS/MS Fragmenta-on of HADEGASKLP Found in 34KD_MYCTU (34 kDa an-genic protein homolog) -‐ Mycobacterium tuberculosis H37Rv 0954
Ions Score: 29
# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 138.0662 69.5367 H 10
2 209.1033 105.0553 A 887.4469 444.2271 870.4203 435.7138 869.4363 435.2218 9
3 324.1302 162.5688 306.1197 153.5635 D 816.4098 408.7085 799.3832 400.1953 798.3992 399.7032 8
4 453.1728 227.0901 435.1623 218.0848 E 701.3828 351.1951 684.3563 342.6818 683.3723 342.1898 7
5 510.1943 255.6008 492.1837 246.5955 G 572.3402 286.6738 555.3137 278.1605 554.3297 277.6685 6
6 581.2314 291.1193 563.2209 282.1141 A 515.3188 258.1630 498.2922 249.6498 497.3082 249.1577 5
7 668.2634 334.6354 650.2529 325.6301 S 444.2817 222.6445 427.2551 214.1312 426.2711 213.6392 4
8 796.3584 398.6828 779.3319 390.1696 778.3478 389.6776 K 357.2496 179.1285 340.2231 170.6152 3
9 909.4425 455.2249 892.4159 446.7116 891.4319 446.2196 L 229.1547 115.0810 2
10 P 116.0706 58.5389 1
MS/MS Fragmenta-on of SLENYIAQT Found in immunogenic protein MPT64 -‐ Mycobacterium tuberculosis H37Rv 1980c
# Immon. a a* a0 b b* b0 Seq. v w w' y y* y0 #
1 60.0444 60.0444 42.0338 88.0393 70.0287 S 9
2 86.0964 173.1285 155.1179 201.1234 183.1128 L 1049.5011 1048.5058 1107.5793 1090.5528 1089.5687 8
3 102.0550 302.1710 284.1605 330.1660 312.1554 E 920.4585 919.4632 994.4952 977.4687 976.4847 7
4 87.0553 416.2140 399.1874 398.2034 444.2089 427.1823 426.1983 N 806.4155 805.4203 865.4526 848.4261 847.4421 6
5 136.0757 579.2773 562.2508 561.2667 607.2722 590.2457 589.2617 Y 643.3522 751.4097 734.3832 733.3992 5
6 86.0964 692.3614 675.3348 674.3508 720.3563 703.3297 702.3457 I 530.2681 543.2885 557.3042 588.3464 571.3198 570.3358 4
7 44.0495 763.3985 746.3719 745.3879 791.3934 774.3668 773.3828 A 459.2310 475.2623 458.2358 457.2518 3
8 101.0709 891.4571 874.4305 873.4465 919.4520 902.4254 901.4414 Q 331.1724 330.1772 404.2252 387.1987 386.2146 2
9 74.0600 992.5047 975.4782 974.4942 1020.4997 1003.4731 1002.4891 T 230.1248 243.1452 245.1244 276.1666 259.1401 258.1561 1
MS/MS Fragmenta-on of LTRDIEEV Found in KatG Mycobacterium tuberculosis H37Rv 1908c
# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 114.0913 57.5493 L 8
2 215.1390 108.0731 197.1285 99.0679 T 1194.5671 597.7872 1177.5405 589.2739 1176.5565 588.7819 7
3 371.2401 186.1237 354.2136 177.6104 353.2296 177.1184 R 1093.5194 547.2633 1076.4929 538.7501 1075.5088 538.2581 6
4 486.2671 243.6372 469.2405 235.1239 468.2565 234.6319 D 937.4183 469.2128 919.4077 460.2075 5
5 599.3511 300.1792 582.3246 291.6659 581.3406 291.1739 I 822.3913 411.6993 804.3808 402.6940 4
6 728.3937 364.7005 711.3672 356.1872 710.3832 355.6952 E 709.3073 355.1573 691.2967 346.1520 3
7 857.4363 429.2218 840.4098 420.7085 839.4258 420.2165 E 580.2647 290.6360 562.2541 281.6307 2
8 956.5047 478.7560 939.4782 470.2427 938.4942 469.7507 V 451.2221 226.1147 433.2115 217.1094 1
Ions Score: 27
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