INVESTIGATION OF TOXICITY OF VARIOUS NANOPARTICLES ON CORD ORIGINATED MESENCHYMAL STEM CELLS OBJECTIVE: Some of the commonly used stem cell components are bone marrow, adipose tissue, cord blood and cord matrix. Isolated cord derived using various methods (cord matrix ) high proliferation potential of mesenchymal stem cells can be applied to toxicity studies. The purpose of this study is to investigate the effect of the nanoparticles such as titanium dioxide, titanium silver, silver and zinc on mesencyhmal stem cells obtained from cord matrix in order to be used in tissue engineering. METHODS: The methods used in the experiments were; isolation (explant), microscopic, culture, cell count and vibiality analysis 3 - (4,5- dimethyltriazole-2-yl) -2,5-diphenyltetrazolium bromide (MTT) and flow cytometric. CONCLUSİON: Due to the lowest nanoparticle toxic effect, titanium silver with the highest EC 50 was determined as the most suitable application for the cord matrix originated mesencyhmal stem cells. The non-toxic concentrations of titanium, titanium silver, silver and zinc nanoparticles to be used as medicine, vaccine and in different fields of tissue engineering. . 5 th INTERNATIONAL CONGRESS OF MOLECULAR MEDICINE, 20– 22 MAY 2015, IZMIR, TURKEY MELIKE ERSOZ 1 , ADIL ALLAHVERDIYEV 2 1 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Bilim University, Istanbul, Turkey 2 Department of Bioengineering, Faculty of Chemical and Metallurgical, Yıldız Technical University, Istanbul, Turkey RESULTS: As a result of the microscopic and flow sytometric analysis, surplus number of healthy mesencyhmal stem cells were isolated from cord matrix using explant method in a short period of time without using enzymes (Figure 1,2). MTT results revealed that titanium, titanium silver, silver and zinc nanoparticles overdose has a toxic effect on cord originated mesencyhmal stem cells as well as decreasing the proliferation. in cord originated mesencyhmal stem cells titanium EC 50 is 20,58, titanium silver EC 50 is 33,3, silver EC 50 is 12,11 and zinc EC 50 is 3,1 (Figure 3). Figure 1: Mesencyhmal stem cells were isolated from cord matrix using explant method 96-hour microscopic image (20x) Figure 2: A result of flow sytometric analysis mesencyhmal stem cells were isolated from cord matrix using explant method Figure 3: In cord matrix originated mesencyhmal stem cells titanium, titanium silver, silver and zinc nanoparticles EC 50 REFERENCES Hee-Hoon, Y., Bo-Young, J., Young-Kwon, S., Kye-Yong, S. ve Jung-Keug, P., (2010). “ In vitro hepatic differentiation of umbilical cord-derived mesenchymal stem cell”, Process Biochemistry, 45: 1857-1864. Bıeback, K., Kern, S., Kluter, H. ve Eichler, H., (2004). “Critical Parameters fort he Isolation of Mesenchymal Stem Cellsfrom Umbilical Cord Blood”, Stem Cells, 22: 625-634. Magrez, A., Kasas, S., Sallicio, V., Pasquier, N., Seo, J.W., Celio, M., Catsicas, S., Schwaller, B. ve Forro, L., (2006). “Cellular toxicity of carbon-based nanomaterials”, Nano Letter, 6 (6): 1121–1125 Hou, Y., Cai, K., Li, J., Chen, X., Lai, M., Hu, Y., Luo, Z., Ding, X. ve Xu, D., (2013). “Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells”, Int J Nanomedicine, 8: 3619-3630.

INVESTIGATION OF TOXICITY OF VARIOUS NANOPARTICLES ON CORD ORIGINATED MESENCHYMAL STEM CELLS OBJECTIVE: Some of the commonly used stem cell components

Download PPT Report

Upload
georgiana-lamb
View
213
Download
1

Tags:

Embed Size (px)

Citation preview

INVESTIGATION OF TOXICITY OF VARIOUS

NANOPARTICLES ON CORD ORIGINATED MESENCHYMAL

STEM CELLS

OBJECTIVE: Some of the commonly used stem cell components are

bone marrow, adipose tissue, cord blood and cord matrix. Isolated cord

derived using various methods (cord matrix ) high proliferation

potential of mesenchymal stem cells can be applied to toxicity studies.

The purpose of this study is to investigate the effect of the nanoparticles

such as titanium dioxide, titanium silver, silver and zinc on

mesencyhmal stem cells obtained from cord matrix in order to be used

in tissue engineering.

METHODS: The methods used in the experiments were; isolation

(explant), microscopic, culture, cell count and vibiality analysis 3 - (4,5-

dimethyltriazole-2-yl) -2,5-diphenyltetrazolium bromide (MTT) and

flow cytometric.

CONCLUSİON: Due to the lowest nanoparticle toxic effect, titanium silver with the highest EC 50 was determined as the most suitable application for

the cord matrix originated mesencyhmal stem cells. The non-toxic concentrations of titanium, titanium silver, silver and zinc nanoparticles to be used

as medicine, vaccine and in different fields of tissue engineering.

5th INTERNATIONAL CONGRESS OF MOLECULAR MEDICINE, 20– 22 MAY 2015, IZMIR, TURKEY

MELIKE ERSOZ1, ADIL ALLAHVERDIYEV2

1 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Bilim University, Istanbul, Turkey

2 Department of Bioengineering, Faculty of Chemical and Metallurgical, Yıldız Technical University, Istanbul, Turkey

RESULTS: As a result of the microscopic and flow sytometric

analysis, surplus number of healthy mesencyhmal stem cells were

isolated from cord matrix using explant method in a short period of

time without using enzymes (Figure 1,2). MTT results revealed that

titanium, titanium silver, silver and zinc nanoparticles overdose has a

toxic effect on cord originated mesencyhmal stem cells as well as

decreasing the proliferation. in cord originated mesencyhmal stem cells

titanium EC50 is 20,58, titanium silver EC50 is 33,3, silver EC50 is 12,11

and zinc EC50 is 3,1 (Figure 3).

Figure 1: Mesencyhmal stem cells were isolated from cord matrix using explant method 96-hour microscopic image (20x)

Figure 2: A result of flow sytometric analysis mesencyhmal stem cells were isolated from cord matrix using explant method

Figure 3: In cord matrix originated mesencyhmal stem cells titanium, titanium silver, silver and zinc nanoparticles EC50

REFERENCES

Hee-Hoon, Y., Bo-Young, J., Young-Kwon, S., Kye-Yong, S. ve Jung-Keug, P., (2010). “ In vitro hepatic differentiation of umbilical cord-derived mesenchymal stem cell”, Process Biochemistry, 45: 1857-1864.

Bıeback, K., Kern, S., Kluter, H. ve Eichler, H., (2004). “Critical Parameters fort he Isolation of Mesenchymal Stem Cellsfrom Umbilical Cord Blood”, Stem Cells, 22: 625-634.

Magrez, A., Kasas, S., Sallicio, V., Pasquier, N., Seo, J.W., Celio, M., Catsicas, S., Schwaller, B. ve Forro, L., (2006). “Cellular toxicity of carbon-based nanomaterials”, Nano Letter, 6 (6): 1121–1125

Hou, Y., Cai, K., Li, J., Chen, X., Lai, M., Hu, Y., Luo, Z., Ding, X. ve Xu, D., (2013). “Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells”, Int J Nanomedicine, 8: 3619-3630.