Introduction to Instruments

FUJI DRI-CHEM 4000i

Scil Vet Focus 5 Cryostat for Frozen Sections

2010/12/06

Speaker ： Le-Shi Tsai

Notice for the use of animal center Instrument

• Please attend illustration meeting before you begin operating instruments. If you do not participate in the illustration meeting , you may NOT use the instrument. (The meeting will be held 2-3 times per semester, and please pay attention to the animal center announcement.)

• Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation.

Charging methods

Instrument Charging methods

FUJI DRI-CHEM 4000iBiochemistry Analyzer

*Less than 3 tests :First hour - $ 400/hour

Additional hours-$ 200 /hour

*More than 3 tests :Each one more test, the first hour charge extra $100

Scil Vet Focus 5 Hematology Analyzer

1 test - $750

each additional test -$100

Leica CM3050 S Cryostat for Frozen Sections

$150/0.5hr

FUJI DRI-CHEM 4000i Biochemistry Analyzer

Main instrument

1. The registration forms 2. Exception code table

Need to purchase reagents by user

Colorimetric

Potentiometric Linear Measurement time

External Structure of the Instrument

廢料盒

Can not open the lid when indication light is on

Light source

Internal Structure of the Instrument

Automatic detection

1. can be automatically diluted 2. A test only needs at least 10μL.（ Manual detection ）

Principle： Colorimetric

Spreading layer：1.filtered out macromolecules (proteins, dyes, etc.)2.evenly spread the fluid sample into the reaction layer

β-D-glucose ＋ O2 ＋ H2O GOD D-gluconic acid＋ H2O2

1,7-Dihydroxynaphthalene ＋ 4-aminoantipyrine ＋H2O2 POD Red dye

505nm

reflection density calibration curve glucose concentration

Principle： Potentiometric

generated voltage difference between ion selective electrodes applied to a reference solution and to the sample

potentiometric difference between the sample and the reference solution is measured

E = E0 + 2.303 * RT/Nf * Log aiai = f * CiCi = Ion concentration

Scil Vet Focus 5 Hematology Analyzer

Introduction to Instrument

• The following animals can be detected: dog 、 rat 、 pig 、 rabbit 、 mouse 、

G. pig

• The following items can be detected: WBC 、 DC 、 RBC 、 HGB 、 HCT 、 MCV 、 MCH 、 MCHC 、 RDW 、 Retics 、PLT 、 MPV 、 PCT

Slide 002

Equipment

main Instrument

printer

the kit

waste pail

the registration forms

Equipment

the kit

1.Diluent2.Clean Deproteinizer3.Cellyse

1. red blood cell lysis 2. reagent to convert all hemoglobin to the stable cyanmethemoglobin form

Principles

感應區

Diluent form a vortex

Cells are injected into the core of a sheath flow and confined to a narrow single-file stream by hydrodynamic focusing(15μm).

The cells are forced to pass through aperture in single file (one by one).

1. A distinguishing feature of blood cell types is

the diameter of the cells.（ RBC 、 WBC 、 Platelet）

2. WBCs are classified according to the characteristics of their cytoplasm and nucleus.3. Cyanmethemoglobin can then be detected by a spectrophotometer set at 540 nm. The concentration of hemoglobin is then calculated from the optical density of the solution.

Specimen preparationSpecimen requirements

1. Use the EDTA anticoagulant 3 ml tubes.

2. Specimen volume: At least 1.5 ml

3. Specimen volume are less than 1.5mL(at least 100 μL):

Use small test tube containing a specimen and add

EDTA as an anti-coagulant (Manual mode).Preservation conditions

Samples can be analyzed within 4 hour(room temperature).

Interfering factors

Measurement results are affected by hemolysis, chyle and clotted blood.

DO NOT use clotted blood specimen.

Automatic Mode

• open the lid

• mix the specimen well

• put your sample into the

specimen- container

housing rack

Leica CM3050 S Cryostat for Frozen Sections

Frozen Tissue Processing

only use O.C.T as embedding medium

Hard tissue using a dedicated film

tissue process and cutting

fixing and embedding

freeze and slice

stain

mounting

observe it under a microscope

The advantage of Leica CM3050 S • It is ideal for undecalcified hard tissue :

1. can be set to any section thickness and

section automatically

2. with a dedicated film, can avoid the hard

tissue broken.• with rough-cut function• adjustable specimen temperature ： In accordance with the type of specimen to select the appropriate

temperature.• adjustable cutting speed

Specifications

Specimen size Maximum 40mm

Section thickness setting 0.5~300μmSectioning speed 0.1~210mm/sSpecimen temperature -10 ~-50 ℃ ℃

supplies you need to purchase

Consumables Suitable specimens

Stage Buy different size according to the size of the specimen

Disposable blades General tissue

Ukrainian carbon blades

Hard tissue

Dedicated film Hard tissue

Thanks for your attention