Chapter 10 Gene Isolation and Manipulation

Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.

Finding specific nucleic acids by using gel electrophoresis and

blotting

Polymerase chain reaction (PCR)

Sequencing

The structure of 2’,3’-dideoxynucleotides

Sanger Sequencing (Dideoxy Sequencing)

The dideoxy sequencing method

Reading the DNA sequence from an automatic sequencer

How to amplify an interesting gene

Making a cDNA clone

Introduction of a transgene

Agrobacterium tumefaciens

causal agent of Crown Gall

The Ti plasmid vector

The generation of a transgenic plant

The generation of a transgenic plant

Pattern of transmission of T-DNA