Introducing The PHERAstar FSX

The NEW Gold Standard for HTS

Catherine Wark ELRIG snapshot April 2016

FSX - Introducing the key features

• The PHERAstar FSX builds on the success of the PHERAstar FS launched in 2009

• All PHERAstar FS features are included in the FSX

• Increased Sensitivity in FI, TRF, TR-FRET, AS, FP & Lumi

• Faster read times in TR-FRET with new faster laser

• Broader Dynamic range in Luminescence (9 decades)

• Improved Alpha Technology performance

• New Applications –Introducing Alphaplex technology with Simultaneous Dual Emmision

Better Sensitivity FI and FP

Plate Format PHERAstar FS PHERAstar FSX Conditions

384 0.4 pM Fluorescein 0.15 pM Fluorescein 20 µL - 384 small volume

1536 2.0 pM Fluorescein 0.5 pM Fluorescein 10 µL - 1536 HiBase

Plate Format PHERAstar FS PHERAstar FSX Conditions

3841.0 mP SD at 1 nM

Fluorescein0.5 mP SD at 1 nM

Fluorescein20 µL - 384 small volume,

15363.0 mP SD at 1 nM

Fluorescein1.5 mP SD at 1 nM

Fluorescein10 µL - 1536 HiBase

Better TRF/TR-FRET Performance

Light Source PHERAstar FS PHERAstar FSX

Xenon Flashlamp 300 flashes / sec 300 flashes / sec

TRF Laser 40 flashes / sec 60 flashes / sec

The energy per Laser flash is the same for PHERAstar FS and PHERAstar FSX

Added Performance50 % more Laser flashes within the same read time per well

50 % higher counts for donor and acceptor

better statistics, higher Z‘Enhanced Speed33 % shorter read time per well at the same number of Laser flashes

speed advantage

shortest 1536 read time with TRF Laser: 36 sec (flying mode, 1 flash)

PHERAstar FSX Lumi & Alpha Performance

PHERAstar FS PHERAstar FSX

Luminescence Dynamic Range 2,000,000 Counts / sec 10,000,000 Counts / sec

Orders Dynamic range 8 Orders 9 Orders

• Simultaneous Dual Emission Detection for Alpha Technology Using AlphaPlex beads

• Improvement to AlphaScreen Sensitivity

• Improvements signal to noise

Luminescence

Alpha Technology

Lumi and Alpha Cross talk reduction

Standard white plate Alpha plate

Advanced Cross Talk reduction wizards built in to avoid Cross talk through the walls of the plate

Summary of improvements

- Significant sensitivity improvements in FI and FP- General Improved HTRF performance with Flash lamp- TRF Nitrogen Laser with 60Hz repetition rate: faster reading- 5 times higher count rate than FS (10 Mio counts/sec):

extended dynamic range in LUM and AlphaTechnologies - Improved crosstalk reduction functionality in LUM & Alpha- Improved Alpha Technologies : Better S/B and S/N - AlphaPlex based on Simultaneous Dual Emission Channel

measurement - New Faster Stacker - New software features including new well scanning options- Increased Calculations options in Analysis package MARS

Acknowledgments

Our Application Specialists and Engineers in Ortenberg, Germany

The PHERAstar FSX roadshow is coming to UK

If interested please visit us at stand D1 and

put us to the test!