ROMANIAN ARCHIVESOF

MICROBIOLOGY AND

IMMUNOLOGY

TOTAL PUBLISHING HOUSE

Founded byPROFESSOR ION CANTACUZINO

VOLUME 70 - No. 4October - December 2011

Published quarterly

by

CANTACUZINO INSTITUTE BUCHAREST

IDeNTIFICATION OF TRePONeMA DeNTICOLA IN sUBGINGIVAL sAMPLes BY PCR TeCHNOLOGY AND ITs CORReLATION WITH CLINICAL DIAGNOsIsBogdan Dabu, Magdalena Mironiuc - Cureu, Dumitru Jardan and Camelia szmal

In vItro AND In vIvo ANTIBACTeRIAL ACTIVITY OF ACORN HeRBAL exTRACT AGAINsT sOMe GRAM-NeGATIVe AND GRAM-POsITIVe BACTeRIAReza Mohebi, sobhan Ghafourian, Zamberi sekawi, Afra Khosravi, elham Abouali Galehdari, Reza Hushmandfar,Reza Ranjbar, Abbas Maleki, Mona Mohammadzadeh, Mohammad Rahbar, Nourkhoda sadeghifard

THe ROLe OF BLAOxA-LIKe CARBAPeNeMAse AND THeIR INseRTION seQUeNCes (Iss) IN THe INDUCTION OF ResIsTANCe AGAINsT CARBAPeNeM ANTIBIOTICs AMONG ACInEtoBACtEr BAUMAnnII IsOLATes IN TeHRAN HOsPITALsKhairollah Asadollahi, eshrat Alizadeh, Mehdi Akbari, Morovat Taherikalani, Mohammad Niakan, Abbas Maleki,Parisa Asadollahi, setareh soroush, Mohammad-Mahdi Feizabadi, Mohammad emaneini

MeTHODOLOGY OPTIMIZATION AND DIVeRsIFICATION FOR THe INVesTIGATION OF VIRULeNCe POTeNTIAL IN HAEMoPHILUS InFLUEnZAE CLINICAL sTRAINsMihaela Cristina Giucã, Monica strãuþ, Maria surdeanu, Maria Nica, Vasilica Ungureanu, Grigore Mihãescu

ANTIBODY AND sPLeNOCYTe PROLIFeRATION ResPONse TO WHOLe INACTIVATeD StrEPtoCoCCUSPnEUMonIAE seROTYPe 1, 3 AND 6B IN MICeMarina Panã, Rasid Orhan, Leontina Bãnicã, Adina Daniela Iancu, Crina stãvaru

GLUTeN sCReeNING OF seVeRAL DIeTARY sUPPLeMeNTs BY IMMUNOCHROMATOGRAPHIC AssAYsimona Oancea, Adriana Wagner, elena Cîrstea, Mirela sima

ROMANIAN exPeRIeNCe IN CHILD CeLIAC DIseAse DIAGNOsIsGabriel samaşca, Mihaela Iancu, Adrian Bãican, Manuela Bruchental, Paraschiva Cherecheş-Panþa, Otilia Fufezan, Angela Butnariu, Dorin Farcãu, Lucia Burac, Tudor Pop, Alexandru Pîrvan, Lucia slãvescu, Miu Nicolae, Andreica Mariana, Matinca Doina, Gheban Dan, Nechit Romulus, Michaela Ponta, Ileana Constantinescu, Doru Dejica, Victor Cristea

sUBJeCT INDex

AUTHOR INDex

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CONTeNTs

MICROBIOLOGY

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Aims and Scoperomanian Archives of Microbiology and Immunoloy, an internationaljournal dedicated to original research work, publishes papers focusingon various aspects of microbiology and immunology. romanian Ar -chives of Microbiology and Immunology is indexed in MeDLINe. Thefrequency of the Journal is currently four issues per year.

Categories of manuscriptsFull-length articles are full-length descriptions of original research (up to10 printed pages).reviews are comprehensive appraisals of research in a field of currentinterest. All reviews are subject to the normal review process (up to 15printed pages).rapid Communications are brief, definitive reports of highly significantand timely findings in the field (up to 5 printed pages).

Submission of manuscriptsManuscripts and all attached files (tables and illustrations) should besubmitted in electronic form to the editorial Office, e-mail address:archives@cantacuzino.ro or by regular mail (address: Redactia RevisteiRomanian Archives of Microbiology and Immunology, spl. Indepen -den tei 103, sector 5, 050096, Bucuresti, Romania) on compact disk,preferrably accompanied by three copies of the manuscript, includingtables and figures, printed on one side of A4 paper format, double-spaced, with 2.5 margins.

The preferred software is Microsoft Word.In order to speed up the process of review, manuscripts should be pre-pared very carefully.

Cover lettereach manuscript submitted to the Romanian Archives of Microbiologyand Immunology must be accompanied by a Cover letter including anexplicit statement by the corresponding author that:• the manuscript represents an original work, has not been previously

published, and has not been submitted simultaneously for publicationelsewhere.

• the manuscript, as submitted, has been reviewed and approved by allnamed authors and that all authors concur with the submission andare responsible for its content.

Editorial review and acceptanceAll manuscripts are subject to editorial review by professional peerreviewers (at least two). The acceptance criteria for all manuscripts arebased on quality and originality. The corresponding author of a manu-script is informed within 45 days after submission that the paper isaccepted for publication in the journal, needs revision or is rejected.Revised manuscripts should be resubmitted as soon as possible but notlater than 14 days.

Ethical considerationsA paper describing any experimental work with humans should includea statement that the ethics Committee of the institution in which thework was done has approved it, and that the subjects gave informedconsent to the work.experiments with animals should be done in accordance with the legalrequirements of the relevant local or national authority. Proceduresshould be such that animals used in experiments do not suffer unneces-sarily. Papers should include details of the procedures and anaestheticsused. The editors will not accept papers where the ethical aspects are,in their opinion, open to doubt.

Preparation of manuscriptsManuscripts should be submitted in english. American or British spellingcan be used provided that only one spelling style is consistently usedthroughout. Manuscripts must be typewritten on A4 format (210x297mm), with double spacing, margins of 25 mm, on one side only, con-secutively numbered. Times New Roman font, 12-point size, is required.

Text headings All headings in the text should be set over to the left hand margin, andtext should begin on the next line. Type first level (sectional) headingsall in capitals. second level headings should be typed in small (lowercase) letters but with the first letter of each main word a capital. For thirdlevel headings, only the first letter of the first word should be a capital.Underline first and second level headings.

FIRsT LeVeL TexT HeADINGsecond Level Text HeadingThird level text heading

Manuscripts should be divided into the following sections and order: Title page, Abstract and key words, Introduction, Materials and Me -thods, Results, Discussion, Acknowledgements, References, Tables,Figure Legends and Figures. 1. Title page contains: title of the paper not longer than 80-100 charac-

ters, including spaces and punctuation; full name of the authors andtheir affiliation; the author responsible for correspondence will bemarked by an asterisk, and his full address telephone/fax numbers,and e-mail address will be indicated.

2. Abstract must not exceed 250 words and should reflect the contentof the study. Following the abstract, a list of 3-10 keywords is essen-tial for indexing purposes.

3. Introduction containing a description of the problem under investi-gation and a brief survey of the existing literature on the subject.

4. Materials and Methods provide sufficient detail to allow the work tobe reproduced.

5. Results. Results should be clear and concise.6. Discussion that enriches but does not repeat section 3 or 5.7. Acknowledgements (if applicable) containing acknowledgement of

technical help and of financial material support.8. References should be numbered consecutively in the order in which

they are first mentioned in the text. Identify references in text, tables,and legends by Arabic numerals in square brackets (e.g. [1], [2-6],etc.). Please note the following examples:Journals: Allain F, Vanpouille C, Carpentier M, Slomianny MC, Durieux S,Spik G. Interaction with glycosaminoglycans is required for cy -clophilin B to trigger integrin-mediated adhesion of peripheral bloodT lymphocytes to extracellular matrix. Proc Natl Acad sci U s A2002. 99: 2714-2719. Books:Theofilopoulos AN. Immune complexes in autoimmunity. In: BonaCA, siminovitch KA, Zanetti M, Theofilopoulos AN (eds.) The Mole -cular Pathology of Autoimmune Diseases. Harwood Academic Pu -blishers, switzerland 1993, pp 229-244.

9. Tables with suitable captions at the top and numbered with Arabicnumerals should be collected at the end of the text on separatesheets (one page per Table). Footnotes to tables should be markedwith a) b) c) etc and *, **, *** should be reserved for p values. eachtable must be understood independently of the text. All tables mustbe cited in the text.

10. Figures (illustrations) Figures should be submitted on separatepages at the end of the article (new page for each complete figure).They should be numbered in the order of their appearance withArabic numerals. Figures should be submitted as TIFF files at a pro perresolution as follows: Graphs at 800-1200 dpi; Photos at 400-800DPI; Color 300-400 DPI. Text in figures should be 8-10 point insize. each figure must have a separate legend. The legends shouldnot appear under the figures, but be gathered in a separate section(Figure legends). Color figures can only be printed if the author isprepared to pay the cost incurred.

11. Figure legends should be supplied at the end of the manuscript,double spaced, with relevant figure numbers, labeling symbol andexplanation.

Units of measurement, Symbols and abbreviations symbols for physical units should be those of the système Internationale(sI) Units.Alternative or non-sI units may be used, but these must be defined attheir first occurrence in the text.

Nomenclature of MicroorganismsBinary names, consisting of a generic name and a specific epithet (e.g.,escherichia coli), must be used for all microorganisms.

Genetic NomenclatureTo facilitate accurate communication, it is important that standard ge -netic nomenclature be used whenever possible and that deviations orproposals for new naming systems be endorsed by an appropriate au -thoritative body.

Proofs and reprintsTen reprints of each article and one copy of the journal will be suppliedfree of charge to the corresponding author.

romanian Archives of Microbiology and Immunology

INsTRUCTIONs TO AUTHORs

REFERENCES:

1. Holt SC, Ebersole JL. The oral spirochetes: Their ecolo-gy and role in the pathogenesis of periodontal disease.In: Radolf JD, Lukehart sA (eds.) Pathogenic Treponema.Molecular and cellular biology. Caister Academic Press,england 2006, pp 323-356.

2. Curtis M, Zenobia C, Darveau R. The relationship of theoral microbiotia to periodontal health and disease. CellHost & Microbe 2011. 10: 302-306.

3. Loesche W.J. The role of spirochetes in periodontaldisease. Adv Dent res 1988. 2: 275-283.

4. Loesche WJ, Syed SA, Schmidt E, Morrison EC. Bacterialprofiles of subgingival plaque in periodontitis. JPeriodontol 1985. 56: 447-456.

5. Keyes PH, Rams TE. A rational for management of perio-dontal disease, rapid identification of microbial “thera-peutic targets” with phase-contrast microscopy. J AmDent Assoc 1983. 106: 803-12.

6. Sela MN. Role of treponema denticola in periodontaldisease. Crit rev oral Biol Med 2001. 12(5): 399-413.

7. Yashida A, Kawada M, Suzuki N, Nakano Y, Oho T etal. TaqMan real-time polymerase chain reaction assay

for the correlation of treponema denticola numberswith the severity of periodontal disease. oral MicrobiolImmunol 2004. 19: 196-200.

8. Wara-aswapati N, Pitiphat W, Chanchaimongkon L,Tawechaisupapong S, Bach JA, Ishikawa I. Red bacte-rial complex is associated with the severity of chronicperiodontitis in a Thai population. oral Dis 2009.15(5):354-9.

9. Masunaga H, Tsutae W, Oh H, Shinozuka N, KishimotoN, Ogata Y. Use of quantitative PCR to evaluate met-hods of bacteria sampling in periodontal patients.Journal of oral Science 2010. 52(4): 615-621.

10. Dumitriu HT, Dumitriu S, Dumitriu AS. Parodon to -logie. Viata medicala romaneasca, Romania 2009, pp189-204.

11. Hyvärinen K, Laitinen S, Paju S, Hakala A, Suominen-Taipale L, Skurnik M, Könönen E, Pussinen PJ.Detection and quantification of five major periodontalpathogens by single copy gene-based real-time PCR.Innate Immun 2009. 15(4): 195-204.

12. Jervøe-Storm PM, AlAhdab H, Koltzscher M, FimmersR, Jepsen S. Quantification of periodontal pathogens

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ABsTRACTtreponema denticola has been associated with gingivitis and chronic periodontitis. The aim of thisstudy was to identify treponema denticola in subgingival samples using PCR technology and tocorrelate it with clinical diagnosis of subjects. The study was carried out on seventy patients (20-84 years of age; mean age, 45.06±12.58) of which 22 individuals with no detectable gingivitis orperiodontitis, 4 subjects with chronic gingivitis and 44 subjects with chronic periodontitis.subgingival plaque samples were collected from five sites in each patient. DNA was extracted fromthe samples using QIAamp® DNA Mini Kit (QIAGeN® ). treponema denticola and other four peri-odontopathogens were found using multiplex polymerase chain reaction followed by a reversehybridization. The relationship between clinical diagnoses and detection of treponema denticolawas determined with Fisher exact test. The results showed significant differences between diag-nostic groups regarding subject proportion. treponema denticola was detected in 2 out of 22 sub-jects with no detectable gingivitis or periodontitis, 2 out of 4 subjects with chronic gingivitis, and40 out of 44 subjects with chronic periodontitis. Our findings suggest that treponema denticola is closely connected to the initiation and pro -gression of periodontal disease.

Bogdan Dabu1*, Magdalena Mironiuc - Cureu2, Dumitru Jardan3 and Camelia Szmal3

1Dpt of Microbiology, Faculty of Dentistry, UMPh “Carol Davila” Bucharest; 2Dpt of Periodontics, Faculty of Dentistry,UMPh “Carol Davila” Bucharest; 3Molecular Biology Laboratory, Medlife Bucharest

Keywords: treponema denticola, periodontitis, polymerase chain reaction

* Corresponding author: Bogdan Dabu, Department of Microbiology, Faculty of Dentistry, UMPh “Carol Davila” Bucharest; Dimitrie Gerotastreet 19-21, Bucharest, sector 1; Tel. +4021.3180757; bgdabu@clicknet.ro.

IDeNTIFICATION OF TRePONeMA DeNTICOLA IN sUBGINGIVAL sAMPLes BY PCR TeCHNOLOGY

AND ITs CORReLATION WITH CLINICAL DIAGNOsIs

by paper point sampling from the coronal and apicalaspect of periodontal lesions by real-time PCR. Clinoral Investig 2010. 14(5): 533-41.

13. Riviere GR, Smith KS, Carranza NJr, Tzagaroulaki E,Kay SL, Dock M. subgingival distribution of t. denti-cola, t. socranskii, and pathogen-related oral spiro-chetes, prevalence and relationship to periodontal sta-tus of sampled sites. J Periodontol 1995. 66: 829-837.

14. Ramseier CA, Kinney JS, Herr AE, Braun T, Sugai JV,Shelburne CA et al. Identification of pathogen andhost-response markers correlated with periodontaldisease. J Periodontol 2008. 80: 436-446.

15. Ito KA, Ishihara K, Tomita S, Kato T, Yamada S.Investigation of subgingival profile of periodontopathicbacteria using polymerase chain reaction. Bull tokyoDent Coll 2010. 51(3):139-144.

16. Mineoka T, Awano S, Rikimaru T, Kurata H, YoshidaA, Ansai T, Takehara T. site-specific development ofperiodontal disease is associated with increased levelsof Porphyromonas gingivalis, tannerela forsythia andtremonema denticola in subginigival plaque. J Perio -dontal 2008. 79: 670-676.

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DABU et al.

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REFERENCES:

1. Denis O, Nonhoff C, Knoop BC, Dubreux BS, StruelensMJ. emergence of vancomycin-intermediate staphylo -coccus aureus in a Belgian hospital: microbiological andclinical features. J Antimicrob Chemother 2002. 50:383-391.

2. Sinclair S. Chinese herbs: a clinical review of Astragalus,Ligusticum, and schizandrae. Alternative MedicineReview 1998. 3: 338–344.

3. Merken HM, Merken CD, Beecher GR. Kinetics methodfor the quantitation of athocyanidins, flavonols, andflavones in foods. Journal of Agricultural and FoodChemistry 2001. 49: 2727-2732.

4. Nychas GJE. Natural antimicrobials from plants. In:Gould, G.W. (ed.), New Methods of Food Preservation.

Blackie Academic and Professional, London, 1995. pp.58-89.

5. Bauer AW, Kirby E, Sherris EM, Turk M. Antibiotic bystandardized single disk method. Am. J. Clin. Path 1996.45: 493-496.

6. Kim J, Marshall MR, Wei CI. Antibacterial activity ofsome essential oil components against five foodbornepathogens. J Agric Food Chem1995. 43: 2839-2845.

7. Hansen MB, Nielsen SE, Berg K. Re-examination and fur-ther development of a precise and rapid dye method formeasuring cell growth/cell kill. J Immunol Methods1989. 119: 203-210.

8. Myhal ML, Laux DC, Cohen PS. Relative colonizing abi -lities of human fecal and K-12 stains of Escherichia coliin the large intestines of streptomycintreated mice. eu ro -pean Journal of Clinical Microbiology 1982. 1:186-192.

* Corresponding author: Nourkhoda sadeghifard, sadeghifard-n@medilam.ac.ir

ABsTRACTBackground: The search for safe and effective antimicrobial agents, which treat, therapeutically andprophylactically, a wide variety of bacterial infections still represents a top priority for the biome -dical field. This study was undertaken to investigate the antimicrobial properties of herbal extract(acorn) against bacterial pathogens in intestinal tract infections in in vitro and in vivo conditions andto study the effect of herbal extracts against bacteria in comparison with current antibiotics. Findings: ethanol extraction of acorn herb (Jaft) were evaluated against Klebsiella pneumoniae,Escherichia coli, Staphylococcus aureus, Salmonella typhi and Pseudomonas aeroginosa in in vitroand in vivo conditions. Minimal Inhibitory Concentration (MIC) was 10 g/ml, 10 g/ml, 5 g/ml,15 g/ml and 15 g/ml for K. pneumoniae, E. coli, S. typhi, S. aureus and P. aeroginosa, respec-tively. The in vivo results showed that the experimental infection produced by K. pneumoniae, E.coli, S. typhi and P. aeroginosa was totally inhibited in rats treated by the acorn extraction, whilepositive control rats died after five days. Conclusion: The finding revealed that acorn extract has great potential as antimicrobial compoundsagainst pathogenic microorganisms. Thus, acorn extract can be used in the treatment of infectiousdiseases caused by resistant bacteria.

In vItro AND In vIvo ANTIBACTeRIAL ACTIVITY OF ACORN HeRBAL exTRACT AGAINsT

sOMe GRAM-NeGATIVe AND GRAM-POsITIVe BACTeRIA

Reza Mohebi1, Sobhan Ghafourian1,2, Zamberi Sekawi2, Afra Khosravi3, Elham Abouali Galehdari1, Reza Hushmandfar4, Reza Ranjbar5, Abbas Maleki1,

Mona Mohammadzadeh6, Mohammad Rahbar7, Nourkhoda Sadeghifard1*

1Clinical Microbiology research Center, Ilam University of Medical Sciences, Ilam, Iran; 2Department of Medical Microbiology & Parasitology, Faculty of Medicine and Health Sciences,

University Putra Malaysia, 43400 Serdang, Selangor, Malaysia; 3Department of Immunology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 4Faculty of veterinary Medicine, Ilam University, Ilam, Iran;

5Biology research Center, Baqiyatallah University of Medical Sciences, tehran, Iran; 6Department of Microbiology, Milad Hospital, tehran, Iran;

7Department of Microbiology, Iranian reference Health Laboratories, tehran, Iran

Keywords: acorn extract, antimicrobial activity, pathogenic bacterial strains

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MOHEBI et al.

9. Alvarez J, Sota M, Vivanco AB, Perales I, Cisterna R,Rementeria AA, Garaizar J. Development of a multiplexPCR technique for detection and epidemiological typingof salmonella in human clinical samples. Journal ofClinical Microbiology 2004. 42: 1734-1738.

10. Anesini, E, Perez, C. screening of plants used in Ar -gentine folk medicine for antimicrobial activity. J. Ethno -pharmacol 1993. 39: 119-128.

11. Martinez MJ, Gonzalez BA, Jauregui A. screening ofsome Cuban medicinal plants for antimicrobial activi-ty. J. Ethnopharmacol 1996. 52: 171-174.

12. Alonso-Paz E, Cerdeiras MP, Fernandez J, Ferreira F,Moyna P, Soubes M, Vazquez A, Veros S, Zunno L.screening of Uruguayan medicinal plants for antimicro-bial activity. J. Ethnopharmacology 1995. 45: 67- 70.

13. Matos FGA, Aguiar LMBA, Silva MGA. Chemical con-stituents and antimicrobial activity of vatairea macro-carpa Ducke. Acta Amazonica 1988. 18: 351-352.

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REFERENCES

1. Wang H, Guo P, Sun H, Yang Q, Chen M, Xu Y, Zhu Y.Molecular epidemiology of clinical isolates of carbapen-em-resistant Acinetobacter spp. from Chinese hospitals.Antimicrob Agents Chemother 2007. 51:4022-4028.

2. Taherikalani M, Fatolahzadeh B, Emaneini M, SoroushS, Feizabadi MM. Distribution of different carbapenemresistant clones of Acinetobacter baumannii in Tehranhospitals. new Microbiol 2009. 32:265-271.

3. Boo TW, Walsh F, Crowley B. Molecular characteriza-tion of carbapenem-resistant Acinetobacter species in an

Irish university hospital: predominance of Acinetobactergenomic species 3. J Med Microbiol 2009. 58:209-216.

4. Brown S, Amyes S. OxA (beta)-lactamases in Acineto -bacter: the story so far. J Antimicrob Chemother 2006.57:1-3.

5. Mugnier PD, Poirel L, Naas T, Nordmann P Worldwidedissemination of the blaOxA-23 carbapenemase gene ofAcinetobacter baumannii. Emerg Infect Dis 2010.16:35-40.

6. Andriamanantena TS, Ratsima E, Rakotonirina HC, Ran -drianirina F, Ramparany L, Carod JF, et al. Dis semi -nation of multidrug resistant Acinetobacter baumannii

ABsTRACTThis study aimed to evaluate the occurrence and dissemination of blaOxA-like carbapenemase genesand their insertion sequences among Acinetobacter baumannii isolates, taken from different hos-pitals in Tehran city and also their roles in the induction of resistance to carbapenem drugs. A total number of 100 non duplicate Acine to bacter baumannii with different origins, were isola -ted from patients with proved nosocomial infections at eight university hospital in Tehran city.Antimi cro bial susceptibility of these strains was done by e-test against 7 antimicrobial agentsaccording to CLsI gui deline. PCR of blaOxA-51-like, blaOxA-23-like, blaOxA-24-like, blaOxA-58-like, ISABA-1,IS1133 was carried out by specia lized primers and then these strains were typed by ReP-finger-printing. Colistin, imipenem and mero penem were the most sensitive antibiotics againstAcinetobacter baumannii isolates with 96%, 51% and 51% sensiti vity respectively. All the isolateshad a blaOxA-51-like intrinsic to these species. The rates of blaOxA-23, 23 and 58-like were 38%, 32% and1% respectively.Coexistence of blaOxA-51/23/24-like was observed among 16% of these isolates. All blaOxA-23-like

carba penemase genes had only one ISABA1. ReP fingerprin ting showed 5 genotypes among car-bapenem resistant isolates, 16 of them being genotype A.This study emphasized on the major role of blaOxA-like carbapenemase, particularly blaOxA-23-like

carbapenemase and their ISABA1, in the dissemination of carbapenem resistant Acinetobacter bau-mannii. This study con firmed a presumptive role of IS element neighbo ring the carbapenemasegene in the elevation of resistance to carbapenem drug among Acinetobacter baumannii isolatesfor the first time in Iran.

THe ROLe OF BLAOxA-LIKe CARBAPeNeMAse AND THeIR INseRTIONseQUeNCes (Iss) IN THe INDUCTION OF ResIsTANCe

AGAINsT CARBAPeNeM ANTIBIOTICs AMONG ACInEtoBACtEr BAUMAnnII IsOLATes IN TeHRAN HOsPITALs

Khairollah Asadollahi1, Eshrat Alizadeh2, Mehdi Akbari3, Morovat Taherikalani2,4*, Mohammad Niakan3, Abbas Maleki4, Parisa Asadollahi4, Setareh Soroush2,4,

Mohammad-Mahdi Feizabadi5, Mohammad Emaneini5

1Department of Epidemiology and Biostatistics, School of Medicine, Ilam University of Medical Sciences; 2Department of Microbiology, School of Medicine, Ilam University of Medical Sciences; 3Department of Microbiology,

School of Medicine, University of Shahed, tehran, Iran; 4Clinical Microbiology research Center, Ilam University ofMedical Sciences, Ilam, Iran; 5Department of Microbiology, School of Medicine, tehran University of Medical Sciences

Keywords: A. baumannii, blaOxA genes, Is element, carbapenem drugs

*Corresponding author: Dr. Morovat Taherikalani (PhD), Assistant Professor in Medical Microbiology, Department of Microbiology, Facultyof Medicine, Ilam University of Medical sciences, Banganjab, Ilam, Iran. Tel: +98 0841-223-5747; Fax: +98 0841-222-7136; e-mail:taherikalani@gmail.com

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8. Akbari M, Niakan M, Taherikalani M, Feizabadi MM,Azadi NA, Soroush S, et al. Rapid identification of Ira nianAcinetobacter baumannii strains by single PCR assayusing BLA oxa-51 -like carbapenemase and evaluationof the antimicrobial resistance profiles of the isolates.Acta Microbiol Immunol Hung 2010. 57 (2):87-94.

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10. Stoeva T, Higgins PG, Savov E, Markovska R, Mitov I,Seifert H. Nosocomial spread of OxA-23 and OxA-58beta-lactamase-producing Acinetobacter baumannii ina Bulgarian hospital. J Antimicrob Chemother 2009.63:618-620.

11. He C, Xie Y, Zhang L, Kang M, Tao C, Chen Z, et al.Increasing imipenem resistance and dissemination ofthe IsAba1-associated blaOxA-23 gene amongAcinetobacter baumannii isolates in an intensive careunit. J Med Microbiol 2010. 60:337-341.

12. Zhou H, Pi BR, Yang Q, Yu YS, Chen YG, Li LJ, ZhengSS. Dissemination of imipenem-resistant Acinetobacterbaumannii strains carrying the IsAba1 blaOxA-23genes in a Chinese hospital. J Med Microbiol 2007.56:1076-1080.

13. Taherikalani M, Etemadi G, Geliani KN, FatollahzadehB, Soroush S, Feizabadi MM. emergence of multi andpan-drug resistance Acinetobacter baumannii carryingblaOxA-type -carbapenemase genes among burn pa -tients in Tehran, Iran. saudi Med J 2008. 29:623-624.

14. Soroush S, Haghi-Ashtiani MT, Taheri-Kalani M,Emaneini M, Aligholi M, Sadeghifard N, et al. Anti mi -crobial resistance of nosocomial strain of Acine to bac -ter baumannii in Children’s Medical Center of Tehran:a 6-year prospective study. Acta Med Iran 2010.48:178-184.

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16. Bou G, Cervero G, Dominguez MA, Quereda C,Martinez-Beltran J. Characterization of a nosocomialoutbreak caused by a multiresistant Acinetobacter bau-mannii strain with a carbapenem-hydrolyzing enzyme:high-level carbapenem resistance in A. baumannii isnot due solely to the presence of beta-lactamases. JClin Microbiol 2000. 38:3299-3305.

17. Taherikalani M, Etemadi G, Geliani KN, FatollahzadehB, Soroush S, Feizabadi MM. emergence of multi andpan-drug resistance Acinetobacter baumannii carryingbla(OxA-type)-carbapenemase genes among burnpatients in Tehran, Iran. Saudi Medical Journal 2008.29:623-624.

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19. Ray A, Perez F, Beltramini AM, Jakubowycz M,Dimick P, Jacobs MR, et al. Use of vaporized hydro-gen peroxide decontamination during an outbreak ofmultidrug-resistant Acinetobacter baumannii infectionat a long-term acute care hospital. Infect Control HospEpidemiol 2010. 31:1236-1241.

20. Oncul O, Ulkur E, Acar A, Turhan V, Yeniz E,Karacaer Z, Yildiz F Prospective analysis of nosocomi-al infections in a burn care unit, Turkey. Indian J Medres 2009. 130:758-764.

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27. Bratu S, Landman D, Martin DA, Georgescu C, QualeJ. Correlation of antimicrobial resistance with beta-lac-tamases, the OmpA-like porin, and efflux pumps inclinical isolates of Acinetobacter baumannii endemicto New York City. Antimicrob Agents Chemother2008. 52:2999-3005.

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ABsTRACTTen Haemophilus influenzae strains were isolated from patients aged between 1.6 - 24 years, withvarious diagnoses (acute meningitis, acute upper respiratory infection, otitis media and acutesinusitis). Identification was based on phenotypic and molecular characteristics; antibiotic suscep-tibility testing was performed by diffusion method according to CLsI standards 2011 for sevenantibiotics. The results of molecular testing showed that all the studied strains produced an ampli-con of 1000 bp with ompP2 primers indicating that all strains were H. influenzae. For six strains,the PCR amplicon obtained with bexA specific primers, proving that the strains were capsulated.The results of phenotypic testing showed that four strains were ampicillin nonsusceptible andb-lactamase-positive. The virulence potential of H. influenzae clinical strains was investigated byphenotypic methods, including the assessment of the soluble virulence factors on specific mediacontaining the biochemical substratum for the investigated enzymatic factor, as well as the adherenceand invasion capacity to HeLa cells monolayer using Cravioto modified method. The studiedstrains exhibited mainly a diffuse adherence pattern and different adherence indexes. Interestingly,two strains isolated from the same pacient (blood and CsF) showed a different degree of invasive-ness, the strain isolated from blood being 20 times more invasive than the one isolated from CsF.

MeTHODOLOGY OPTIMIZATION AND DIVeRsIFICATION FOR THe INVesTIGATION OF VIRULeNCe POTeNTIAL

IN HAEMoPHILUS InFLUEnZAE CLINICAL sTRAINs

Mihaela Cristina Giucã1,2*, Monica Strãuþ1, Maria Surdeanu1, Maria Nica3, Vasilica Ungureanu1, Grigore Mihãescu2

1“Cantacuzino” national Institute of research-Development for Microbiology and Immunology, Bucharest;2Microbiology Department, Faculty of Biology, University of Bucharest;

3tropical and Infectious Diseases Hospital “Dr. v. Babeş”, Bucharest

Keywords: Haemophilus influenzae, serotyping, antibiotic resistance, b-lactamase, PCR capsular type, adhesion,invasion.

*Corresponding author: Mihaela Cristina Giucã, e-mail: giucamihaela@yahoo.com

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ABsTRACTAnimal models of infection and protection on the topic of the Streptococcus pneumoniae (S. pneu-moniae) have encountered many difficulties generated by low immunogenicity, a characteristic ofpolysaccharide capsular bacteria and difference of virulence between serotypes and strains. Wehave explored the immune response after immunization with heat inactivated S. pneumoniae se -rotype 1, 3 and 6B in C57BL/6 mice by IgM and IgG detection, and by splenocyte in vitro 5-ethynyl-2'-deoxyuridine (edU) incorporation after antigen specific stimulation, as a proposed method of cel-lular immune response evaluation. Antibody titer persistence after immunization was not lengthywhile antigen specific proliferation response detected by edU assay was remnant. Intraperitoneal(i.p.) challenge with serotype 6B S. pneumoniae proved that antibody titers and the detected spe-cific cellular immune response do not cover seroprotective necessity and do not confer improvedimmunologic memory in comparison to non-immunized mice, which show natural resistance.

ANTIBODY AND sPLeNOCYTe PROLIFeRATION ResPONse TO WHOLe INACTIVATeD

StrEPtoCoCCUS PnEUMonIAE seROTYPe 1, 3 AND 6B IN MICe

Marina Panã, Rasid Orhan, Leontina Bãnicã, Adina Daniela Iancu, Crina Stãvaru*

Cantacuzino national Institute of research-Development for Microbiology and Immunology, Bucharest, romania

Keywords: Streptococcus pneumoniae, mice, immunization, challenge, edU

* Corresponding author: Crina stãvaru, e-mail address: scrina@cantacuzino.ro; Phone: +4021 3069263; Fax: +4021 3069307; Mobile: +40722662609; Postal address: Cantacuzino National Institute of Research - Development forMicrobiology and Immunology; 103 splaiul Independentei, CP 1-525, code 050096; Bucharest, Romania

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Antibody and splenocyte proliferation response to whole inactivated Streptococcus pneumoniae serotype 1, 3 and 6B in mice

whole cells prevents colonization and invasive diseaseby capsulated pneumococci. Infect Immun 2001.69(8): 4870-3.

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18. Training manual for enzyme linked immunosorbentassay for the quantitation of Streptococcus pneumoni-ae serotype specific IgG (Pn Ps eLIsA)- revision 2006.

19. Crawford MP, Yan SX, Ortega SB, Mehta RS, HewittRE, Price DA et al. High prevalence of autoreactive,neuroantigen-specific CD8+ T cells in multiple sclero-sis revealed by novel flow cytometric assay. Blood2004. 103: 4222-31.

20. Hvalbye BKR, Aaberge IS, Løvik M, Haneberg B. Intranasalimmunization with heat-inactivated streptococcuspneumoniae protects mice against pneumococcalinfection. Infect Immun 1999. 67(9):4320-5

21. Jansen WTM, Hogenboom S, Thijssen MJL, KamerlingJP, Vliegenthart JFG Verhoef J. synthetic 6B Di-, Tri-,and Tetrasaccharide-protein conjugates contain pneu-mococcal type 6A and 6B common and 6B-specificepitopes that elicit protective antibodies in mice. InfectImmun 2001. 69(2):787-93

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12-year-olds born during an epidemic. J Pediatr Gas tro -enterol nutr 2009. 49: 170-176.

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14. Valdes I, Garcia E, Llorente M, Mendez E. Innovativeapproach to low-level gluten determination in foodsusing a novel sandwich enzyme-linked immunosor-bent assay protocol. Eur J Gastroenterol Hepatol 2003.15: 465-474.

ABsTRACTCeliac disease (CD) is a chronic intestinal disorder of public health concern caused by gluten inges-tion in sensitive individuals. Gluten is a protein found not only in gluten-containing food but alsoas normal component of drugs and dietary supplements. Detection of gluten in dietary supple-ments is a very important task required for establishing their gluten status, which is highly impor-tant for the safety of products consumed by CD and gluten-sensitive patients.In this paper, we investigated the presence of gluten in twenty one common dietary supplementsfrom the national market using the immunochromatographic assay. This visual assay proved to bean efficient rapid tool for gluten screening as an alternative to the eLIsA techniques. The resultshave shown the presence of gluten in 23.8% of the investigated samples (vitamins, minerals, plantextracts, probiotics supplements, lactoferrin, propolis supplements). The results provide informa-tion which may contribute to the completion of the existing lists of gluten-free pharmaceuticals. Itis known that for CD patients obtaining accurate information about the gluten content of a parti -cular item is a difficult and time-consuming process.

Simona Oancea*, Adriana Wagner, Elena Cîrstea, Mirela Sima

Lucian Blaga University of Sibiu, Department of Biochemistry and toxicology, Faculty of Agricultural Sciences, Food Industry and Environmental Protection, Sibiu, romania

Keywords: Celiac disease, gluten, immunochromatography, dietary supplements

* Corresponding author: simona Oancea, Tel.: +40269-211338, Fax: +40269-212558, e-mail: simona.oancea@ulbsibiu.ro

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GLUTeN sCReeNING OF seVeRAL DIeTARY sUPPLeMeNTs BY IMMUNOCHROMATOGRAPHIC AssAY

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Gluten screening of several dietary supplements by immunochromatographic assay

15. Ribes-Koninckx C, Ferre-Lopez S, Genzor C, Gamen S,Pena L, Ortigosa L, Mendez E. High efficiency of aNew Generation of Immuno-chromatographic Visualstick Assays for CD screening. J Ped Gastroenterolnutr 2003. 36: 536.

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17. Laube T, Kergaravat SV, Fabiano SN, Hernández SR,Alegret S, Pividori MI. Magneto immunosensor forgliadin detection in gluten-free foodstuff: Towards foodsafety for celiac patients. Biosensors & Bioelectronics2011. 27(1): 46-52.

18. Sorell L, Lopez JA, Valdes I, Alfonso P, Camafeita E,Ace vedo B, Chirdo F, Gavilondo J, Mendez E. Aninnovative sandwich eLIsA system based on an anti-body cocktail for gluten analysis. FEBS Lett 1998. 439:46-50.

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6. Samaşca G, Iancu M, Farcãu D, Butnariu A, Pop T,

Pîrvan A, Andreica M, Miu N, Cristea V, Dejica D. IgAantitissue transglutaminase antibodies, first line in celiacdisease diagnosis. Clinical Laboratory Germany 2011;57:695-701.

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9. Catassi C, Fasano A - Celiac disease diagnosis: simplerules are better than complicated algorithms. Am J Med2010; 123: 691-693.

10. Samaşca G, Iancu M, Butnariu A, Andreica M, Con -stantinescu I, Dejica D. The importance of determin-ing human leukocyte antigens in the prevention ofintestinal lymphoma in patients with celiac disease.

ABsTRACTLast consensus in celiac disease in 2008 conducted under the aegis of the european society ofPediatric Gastroenterology, Hepatology and Nutrition jointly with North American society ofPediatric Gastroenterology, Hepatology and Nutrition reveals the following: “celiac disease is achronic immune-mediated enteropathy characterized by sensitization to gluten. that can affectany organ or system, with a wide range of clinical manifestations of variable severity”. Thus, inrecent years, clinical picture of celiac disease has changed the old paradigm - bowel disease withvillous atrophy and malnutrition, being replaced with the new paradigm - multi-organ autoimmunedisease, affecting many organs and systems throughout but with more less specific symptoms,which undiagnosed leads to delayed diagnosis, at a late-onset disease and long-term major com-plications as the risk of cancer. According to this consensus „the serological diagnosis of celiac dis-ease is based on high sensitivity and specificity tests”, but in line with changing clinical features ofceliac disease, its diagnosis has undergone significant changes in recent years. These changes inthe diagnosis of celiac disease, we have decided to analyze them.

ROMANIAN exPeRIeNCe IN CHILD CeLIAC DIseAse DIAGNOsIs

Gabriel Samaşca1, Mihaela Iancu2, Adrian Bãican3, Manuela Bruchental4, Paraschiva Cherecheş-Panþa4, Otilia Fufezan4, Angela Butnariu4, Dorin Farcãu4, Lucia Burac5, Tudor Pop5, Alexandru Pîrvan5,

Lucia Slãvescu5, Nicolae Miu5, Mariana Andreica5, Doina Matinca6, Dan Gheban7, Romulus Nechit8, Michaela Ponta8, Ileana Constantinescu9, Doru Dejica1, Victor Cristea1

1Department of Immunology; 2Department of Medical Informatics and Biostatistics; 3Department of Dermatology; 4Department of Pediatrics III; 5Department of Pediatrics II;

6Department of Microbiology; 7Department of Pathological Anathomy; „Iuliu Haþieganu” University of Medicine and Pharmacy, Cluj-napoca, romania; 8Department of modern methods for quality control,

Faculty of Chemistry; „Babeş-Bolyai” University, Cluj-napoca, românia; 9Diagnostic Molecular Biology Laboratory,Immunology, HLA and virology; „Carol Davila” University of Medicine and Pharmacy Bucharest, românia

Keywords: complex analysis, diagnostic tests, celiac disease, child

*Corresponding author: Dr. Gabriel samaºca - Department of Immunology, Croitorilor street, 19-21, ”Iuliu Haþieganu” University ofMedicine and Pharmacy Clu-Napoca, Romania. e-mail: gabriel.samasca@umfcluj.ro

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scientific Annals of University “Alexandru Ioan Cuza“,section II a. Genetics and Molecular Biology 2010. 11:81-87.

11. Srivastava A, Yachha SK, Mathias A, Parveen F,Poddar U, Agrawal S. Prevalence, human leukocyteantigen typing and strategy for screening among Asianfirst-degree relatives of children with celiac disease. JGastroenterol Hepatol 2010; 25:319-324.

12. Samaşca G, Iancu M, Butnariu A, Pîrvan A, AndreicaM, Miu N, Constantinescu I, Dejica D. Controversiesin the laboratory diagnosis of celiac disease in chil-dren. New haplotypes discovered. romanian Archivesof Microbiology and Immunology 2010. 69: 119-124.

13. Fasano A, Araya M, Bhatnagar S, Cameron D, CatassiC, Dirks M, Mearin ML, Ortigosa L, Phillips A; CeliacDisease Working Group, FISPGHAN. Consensusguidelines. J Pediatr Gastroenterol nutr., UsA, 2008.47: 214-219.

14. Samaşca G, Iancu M, Pîrvan A, Andreica M, Miu N,Constantinescu I, Cristea V, Dejica D. Histocom pa -tibility risk antigens in celiac disease in children. ClujMedical Journal 2011. 84: 269-273.

15. Snyder CL, Young DO, Green PHR, Taylor AK. CeliacDisease. In: Pagon RA, Bird TC, Dolan CR, StephensK, editors. GeneReviews [Internet]. seattle (WA): Uni -versity of Washington, seattle; 1993-2008 Jul 03.

16. Samaşca G, Iancu M, Butnariu A, Andreica M, DejicaD. Unfavorable prognostic markers in association withtype 1 diabetes mellisus-celiac disease. Acta Medicatransilvanica 2010. 2: 66-68.

17. Karavanaki K, Kakleas K, Paschali E, Kefalas N, Kon -stantopoulos I, Petrou V, Kanariou M, Karayianni C.screening for associated autoimmunity in children andadolescents with type 1 diabetes mellitus (T1DM).Horm res. 2009;71:201-6.

18. Samaşca G, Iancu M, Butnariu A, Andreica M, DejicaD. Prevalence of IgA antitissue transglutaminase anti-bodies in children with type 1 diabetes mellitus.Journal of Clinical Laboratory Analysis s.U.A. 2011.25: 156-161.

19. Cev E, Pascu O, Serban V, Mulder CJJ, Taban S,Samaşca G. The Prevalence of Celiac Disease in Adultand Adolescent Romanian Patients With Type 1Diabetes Mellitus. tMJ 2010; 60: 155-160.

20. Samaşca G, Bãican A, Dejica D, Miu N. Rare Diseases:Dermatitis herpetiformis. Description, laboratory diag-nosis, stage results. Journal of Medicine and PharmacyOrvosi often Gzogzszereszeti szemle 2009. 55: 300-302.

21. James SP. This month at the NIH: Final statement ofNIH Consensus Conference on Celiac disease Gastro -enterology 2005, 128-136.

22. Samaşca G, Bãican A, Pîrvan A, Dejica D. DermatitisHerpetiformis. romanian Journal of Medical Labo -ratory 2009. 4: 23-27.

23. Livia Van. Dermatitis herpetiformis: Potential for con-fusion with linear IgA bullous dermatosis on directimmunofluorescence. Dermatology online Journal2008;14:21.

24. Samaşca G, Nechit R, Ponta M, Iancu M, Butnariu A,Farcãu D, Andreica M, Dejica D. Biochemical nutri-tional status in children with celiac disease. Cluj Me -dical Journal 2010. 83: 497-503.

25. Kuloğlu Z, Kirsaçlioğlu CT, Kansu A, Ensari A, GirginN. Celiac disease: presentation of 109 children. YonseiMed J 2009;50:617-623

26. Samaşca G, Dejica D. Ige-mediated immune responsein celiac disease in children. romanian MedicalJournal 2010; 57: 66-69.

27. Silano M, Di Benedetto R, Maialetti F, De Vincenzi A,Calcaterra R, Trecca A, De Vincenzi M. 10-residuepeptide from durum wheat promotes a shift from aTh1-type response toward a Th2-type response in celi-ac disease. Am J Clin nutr 2008; 87: 415-423.

28. Samaşca G, Pîrvan A, Miu N, Andreica M, Cristea V,Dejica D. Adenoviruses in celiac disease in children.romanian Journal of Infectious Diseases 2011. 14: 5-9.

29. Barbeau WE, Novascone MA, Elgert KD. Is celiac dis-ease due to molecular mimicry between gliadin pep-tide-HLA class II molecule-T cell interactions and thoseof some unidentified superantigen? Mol Immunol1997; 34 :535-541.

30. Samaşca G, Burac L, Farcãu D, Dejica D. screening forceliac disease in chronic viral hepatitis C. RomanianJournal of Pediatrics 2010. 59: 49-50.

31. Silano M, Volta U, Vincentini O, De Vincenzi M.Clinical features of chronic C virus hepatitis in patientswith celiac disease. For the Italian Registry of theComplications of Celiac Disease. Eur J Clin MicrobiolInfect Dis 2009; 28:1267-1269.

32. Samaşca G, Bãican A, Pop T, Pîrvan A, Miu N,Andreica M, Cristea V, Dejica D. IgG-F-actin antibo -dies in celiac disease and dermatitis herpetiformis.romanian Archives of Microbiology and Immunology2010. 69: 177-182.

33. Bonaci-Nikolic B, Andrejevic S, Radlovic N, DavidovicI, Sofronic L, Spuran M, Micev M, Nikolic MM. sero -logical and clinical comparison of children and adultswith anti-endomysial antibodies. J Clin ImmunolNetherlands 2007. 27 :163-171.

34. Samaşca G, Cherecheş-Panþa P, Fufezan O, Farcãu D,Gheban D, Cristea V. Celiac disease and VariableImmunodeficiency. romanian review of LaboratoryMedicine 2011. 19: 95-99.

35. Luzi G, Zullo A, Iebba F, Rinaldi V, Sanchez Mete L,Muscaritoli M, Aiuti F. Duodenal pathology and clini-cal-immunological implications in common variableimmunodeficiency patients. Am J Gastroenterol. 2003;98:118-121.

36. Samaşca G, Iancu M, Pop T, Butnariu A, Andreica M,Miu N, Cristea V, Dejica D. The importance of envi-ronmental education in the development of celiac dis-ease. LabMedicine s.U.A. 2011. 42: 497-501.

37. Olsson C, Hörnell A, Ivarsson A, Sydner YM. Theeveryday life of adolescent coeliacs: Issues of impor-tance for compliance with the gluten-free diet. J Humnutr Diet 2008; 21: 359-367.

ROMANIAN ARCHIVes OF MICROBIOLOGY AND IMMUNOLOGY

187

MICROBIOLOGY

MITOCHONDRIAL DNA MUTATIONs IN PATIeNTs WITH HRHPV-ReLATeD CeRVICAL LesIONs

Cristina D. Goia-Ruşanu, Iulia V. Iancu, Anca Botezatu, Demetra socolov,

Irina Huicã, Adriana Pleşa, Gabriela Anton

In vItro ANTIMICROBIAL ACTIVITY OF ROMANIAN MeDICINAL PLANTs

A.M. stanciuc, A. Gaspar, L. Moldovan, C. saviuc, M. Popa, L. Mãruþescu

ANTIMICROBIAL AND ANTI-PATHOGeNIC ACTIVITY OF sOMe THIOUReIDes DeRIVATIVes

AGAINsT ErWInIA AMYLovorA PHYTOPATHOGeNIC sTRAINs

Luminiþa Mãruþescu, Mihai-George Niþulescu, Marcela Bucur, Lia-Mara Diþu,

Grigore Mihãescu, Veronica Lazãr, Tatiana sesan

PROBIOTICs – AN ALTeRNATIVe TReATMeNT FOR VARIOUs DIseAses

Nicoleta Vasile, Raluca Ghidea, Tatiana Vassu

HELICoBACtEr PYLorI CULTIVATION FROM GAsTRIC BIOPsIes AND sUsCePTIBILITY

TO ANTIBIOTICs UseD IN eMPIRICAL THeRAPY

Mãdãlina Ilie, Marcela Popa, Mariana Carmen Chifiriuc, Alina Baltac,

Gabriel Constantinescu, Coman Tãnãsescu

sCReeNING FOR GROUP B sTRePTOCOCCUs: A PRIVATe LABORATORY exeRIeNCe

Violeta-Corina Cristea, Maria Duþã, Gabriela Neacşu

OPTIMIZATION OF TRIPLex ReAL TIMe PCR FOR DeTeCTING StAPHYLoCoCCUS AUrEUS mecA,

pvl AND nuc GeNes

Teodora Vremerã, Luminiþa smaranda Iancu, Cãtãlina Logigan, eduard Nãstase,

egidia Miftode, Cãtãlina Luncã, Olivia Dorneanu

A sTUDY ON APOPTOsIs INDUCING eFFeCTs OF UVB IRRADIATION

IN PSEUDoMonAS AErUGInoSA

Payam Behzadi and elham Behzadi

sURVIVAL OF H5N1 INFLUeNZA VIRUs IN WATeR AND ITs INACTIVATION BY CHeMICAL MeTHODs

Maria elena Mihai, Cristina þecu, Alina elena Ivanciuc, Gheorghe Necula,

emilia Lupulescu, Adrian Onu

sUBJeCT INDex

54

49

11

5

60

65

69

74

78

188

ROMANIAN ARCHIVes OF MICROBIOLOGY AND IMMUNOLOGY

IMMUNOLOGICAL AsPeCTs IN VIRAL HePATITIs B AND C INFeCTION

Irena Manea, Cristian Nicolae Manea, Nicolae Miron, Victor Cristea

CORReLATION OF ANTI-HELICoBACtEr PYLorI CagA IgG ANTIBODIes WITH ResIsTANCe

TO FIRsT LINe TReATMeNT, BLeeDING GAsTRODUODeNAL ULCeRs AND GAsTRIC CANCeR

Mãdãlina Ilie, Luminiþa Dascãlu, Carmen Chifiriuc, Marcela Popa, Gabriel Constantinescu,

Coman Tãnãsescu, Alina Baltac

INFLUeNCe OF ORTHODONTIC TReATMeNT ON ORAL sTRePTOCOCCI

Theodor-Cristian Vizitiu, Mihaela Cristina Giucã, ecaterina Ionescu

IDeNTIFICATION OF TRePONeMA DeNTICOLA IN sUBGINGIVAL sAMPLes BY

PCR TeCHNOLOGY AND ITs CORReLATION WITH CLINICAL DIAGNOsIs

Bogdan Dabu, Magdalena Mironiuc - Cureu, Dumitru Jardan and Camelia szmal

In vItro AND In vIvo ANTIBACTeRIAL ACTIVITY OF ACORN HeRBAL exTRACT

AGAINsT sOMe GRAM-NeGATIVe AND GRAM-POsITIVe BACTeRIA

Reza Mohebi, sobhan Ghafourian, Zamberi sekawi, Afra Khosravi,

elham Abouali Galehdari, Reza Hushmandfar, Reza Ranjbar, Abbas Maleki,

Mona Mohammadzadeh, Mohammad Rahbar, Nourkhoda sadeghifard

THe ROLe OF BLAOxA-LIKe CARBAPeNeMAse AND THeIR INseRTION seQUeNCes (Iss)

IN THe INDUCTION OF ResIsTANCe AGAINsT CARBAPeNeM ANTIBIOTICs

AMONG ACINeTOBACTeR BAUMANNII IsOLATes IN TeHRAN HOsPITALs

Khairollah Asadollahi, eshrat Alizadeh, Mehdi Akbari, Morovat Taherikalani,

Mohammad Niakan, Abbas Maleki, Parisa Asadollahi, setareh soroush,

Mohammad-Mahdi Feizabadi, Mohammad emaneini

MeTHODOLOGY OPTIMIZATION AND DIVeRsIFICATION FOR THe INVesTIGATION

OF VIRULeNCe POTeNTIAL IN HAEMoPHILUS InFLUEnZAE CLINICAL sTRAINs

Mihaela Cristina Giucã, Monica strãuþ, Maria surdeanu, Maria Nica,

Vasilica Ungureanu, Grigore Mihãescu

97

105

145

149

153

159

101

ROMANIAN ARCHIVes OF MICROBIOLOGY AND IMMUNOLOGY

189

IMMUNOLOGY

AssessING THe eFFICIeNCY OF FRee LIGHT CHAIN AssAY IN MONITORING PATIeNTs

WITH MULTIPLe MYeLOMA BeFORe AND AFTeR AUTOLOGOUs sTeM CeLL TRANsPLANTATION

ALONG WITH seRUM PROTeIN eLeCTROPHOResIs AND seRUM PROTeIN IMMUNOFIxATION

Monica Dogaru, Veronica Lazãr, Daniel Coriu

IMMMUNODeTeCTION OF ADDeD GLYCOMACROPePTIDe IN MILK FORMULAs

AND MILK POWDeRs

simona Oancea and Mihaela stoia

BACTeRIAL exTRACT CANTAsTIM ACTIVATes MACROPHAGes VIA TLR-2

Iuliana Caraş, Cãtãlin þucureanu, Ramona Pitica and Aurora sãlãgeanu

IMPLANT MIROPARTICLes – A NeW CONCePT FOR NON-INVAsIVe CANCeR THeRAPY

Carmen Cristina surdu-Bob, Cristin Coman, Ana Cãlugãru,

Lidia Cremer, Marius Bãdulescu, ene Vlase

DYNAMICs OF eNDOTHeLIAL PROGeNITOR CeLLs FOLLOWING seVOFLURANe

PReCONDITIONING

Mihaela Popescu, Adelina Munteanu, Gheorghiþa Isvoranu, Laura suciu,

Bogdan Pavel, Bogdan Marinescu, Leon Zagrean

A HIGHLY PURIFIeD VeGeTAL FRACTION ABLe TO MODULATe HMGB1

AND TO ATTeNUATe sePTIC sHOCK IN MICe

Natalia simona Apetrei, Ana Cãlugãru, F. Kerek, Minerva Panteli, I. Rasit,

Lidia Cremer, G. szegli, Andreea-Roxana Lupu

Is INTeRLeUKIN-17 A PROATHeROGeNIC BIOMARKeR?

Cristina-sorina Cãtanã, Victor Cristea, Nicolae Miron, Ioana Berindan Neagoe

HePATITIs B, C AND D COINFeCTION IN HIV-INFeCTeD PATIeNTs: PReVALeNCe AND PROGRess

Bogdan Ionescu and Grigore Mihãescu

15

28

37

109

114

124

129

23

190

ROMANIAN ARCHIVes OF MICROBIOLOGY AND IMMUNOLOGY

ANTIBODY AND sPLeNOCYTe PROLIFeRATION ResPONse TO WHOLe INACTIVATeD

StrEPtoCoCCUS PnEUMonIAE seROTYPe 1, 3 AND 6B IN MICe

Marina Panã, Rasid Orhan, Leontina Bãnicã, Adina Daniela Iancu, Crina stãvaru

GLUTeN sCReeNING OF seVeRAL DIeTARY sUPPLeMeNTs BY

IMMUNOCHROMATOGRAPHIC AssAY

simona Oancea, Adriana Wagner, elena Cîrstea, Mirela sima

ROMANIAN exPeRIeNCe IN CHILD CeLIAC DIseAse DIAGNOsIs

Gabriel samaşcã, Mihaela Iancu, Adrian Bãican, Manuela Bruchental, Paraschiva Cherecheş-Panþa,

Otilia Fufezan, Angela Butnariu, Dorin Farcãu, Lucia Burac, Tudor Pop, Alexandru Pîrvan,

Lucia slãvescu, Nicolae Miu, Mariana Andreica, Doina Matinca, Dan Gheban, Romulus Nechit,

Michaela Ponta, Ileana Constantinescu, Doru Dejica, Victor Cristea

ReVIeW

NeW INTeRFeRONs IN THe TReATMeNT OF CHRONIC HePATITIs C

simona Ruþã and Costin Cernescu

THe GUT MICROBIOTA IN THe MeTAGeNOMICs eRA: sOMeTIMes A FRIeND, sOMeTIMes A FOe

Daniela elena Şerban

168

174

178

85

134

ROMANIAN ARCHIVes OF MICROBIOLOGY AND IMMUNOLOGY

191

AAkbari Mehdi 153Alizadeh eshrat 153Anca Botezatu 5Andreica Mariana 178Anton Gabriela 5Apetrei Natalia simona 114Asadollahi Khairollah 153Asadollahi Parisa 153

BBaltac Alina 60, 101Bãnicã Leontina 168Bãdulescu Marius 37Bãican Adrian 178Behzadi elham 74Behzadi Payam 74Bruchental Manuela 178Bucur Marcela 49Burac Lucia 178Butnariu Angela 178

CCaraş Iuliana 28Cãlugãru Ana 37, 114Cãtanã Cristina-sorina 124Cernescu Costin 85Cherecheş-Panþa Paraschiva 178Chifiriuc Carmen 60, 101Cîrstea elena 174Coman Cristin 37Constantinescu Gabriel 60, 101Constantinescu Ileana 178Coriu Daniel 15Cremer Lidia 37, 114Cristea Victor 97, 124, 178Cristea Violeta-Corina 65

DDabu Bogdan 145Dascãlu Luminiþa 101Dejica Doru 178Diþu Lia-Mara 49Dogaru Monica 15Dorneanu Olivia 69Duþã Maria 65

Eemaneini Mohammad 153

FFarcãu Dorin 178Feizabadi Mohammad-Mahdi 153Fufezan Otilia 178

GGalehdari elham Abouali 149Gaspar A. 11Ghafourian sobhan 149Gheban Dan 178Ghindea Raluca 54Giucã Mihaela Cristina 105, 159Goia-Ruşanu D. Cristina 5

HHuicã Irina 5Hushmandfar Reza 149

IIancu Adina Daniela 168Iancu Luminiþa smaranda 69Iancu Mihaela 178Iancu V. Iulia 5Ilie Mãdãlina 60Ilie Mãdãlina 101Ionescu Bogdan 129Ionescu ecaterina 105Isvoranu Gheorghiþa 109Ivanciuc Alina elena 78

JJardan Dumitru 145

KKerek F. 114Khosravi Afra 149

LLazãr Veronica 15, 49Logigan Cãtãlina 69Luncã Cãtãlina 69

AUTHOR INDex

192

ROMANIAN ARCHIVes OF MICROBIOLOGY AND IMMUNOLOGY

Lupu Andreea-Roxana 114Lupulescu emilia 78

MMaleki Abbas 149, 165Manea Cristian Nicolae 97Manea Irena 97Marinescu Bogdan 109Matinca Doina 178Mãruþescu L. 11, 49Miftode egidia 69Mihaela stoia 23Mihai Maria elena 78Mihãescu Grigore 49, 129, 159Miron Nicolae 97, 124Mironiuc - Cureu Magdalena 145Miu Nicolae 178Mohammadzadeh Mona 149Mohebi Reza 149Moldovan L. 11Munteanu Adelina 109

NNãstase eduard 69Neacşu Gabriela 65Neagoe Ioana Berindan 124Nechit Romulus 178Necula Gheorghe 78Niakan Mohammad 153Nica Maria 159Niþulescu Mihai-George 49

OOancea simona 23, 174Onu Adrian 78Orhan Rasid 168

PPanã Marina 168Panteli Minerva 114Pavel Bogdan 109Pitica Ramona 28Pîrvan Alexandru 178Pleşa Adriana 5Ponta Michaela 178Pop Tudor 178Popa M. 11, 60, 101Popescu Mihaela 109

RRahbar Mohammad 149Ranjbar Reza 149

Rasit I. 114Ruþã simona 85

Ssadeghifard Nourkhoda 149samaşca Gabriel 178saviuc C. 11sãlãgeanu Aurora 28sekawi Zamberi 149sesan Tatiana 49sima Mirela 174slãvescu Lucia 178socolov Demetra 5soroush setareh 153stanciuc A.M. 11stavaru Crina 168strãuþ Monica 159suciu Laura 109surdeanu Maria 159surdu-Bob Carmen Cristina 37szegli G. 114szmal Camelia 145

ŞŞerban Daniela elena 134

TTaherikalani Morovat 153Tãnãsescu Coman 60, 101

Þþecu Cristina 78þucureanu Cãtãlin 28

UUngureanu Vasilica 159

VVasile Nicoleta 54Vassu Tatiana 54Vizitiu Theodor-Cristian 105Vlase ene 37Vremerã Teodora 69

WWagner Adriana 174

ZZagrean Leon 109