Ecotoxicology LaboratoryEcotoxicology LaboratoryIntegrated in Departamento de Protecção

das Plantas e de Fitoecologia (DPPF)

Sustainable Use of Pesticides: Sustainable Use of Pesticides: Protection of Plants, Human Health, Natural Resources Protection of Plants, Human Health, Natural Resources

d Bi di itd Bi di itand Biodiversityand Biodiversity

Pesticide Risk Management through DecisionPesticide Risk Management through Decision--Making Making S t T lS t T lSupport ToolsSupport Tools

The Ecotoxicology Laboratory is comprised of The Ecotoxicology Laboratory is comprised of t itt ittwo units: two units:

Unit I:Unit I:Pesticide residues analysis

Unit II:Toxic effects evaluationToxic effects evaluation

Unit I: Pesticide Residues AnalysisUnit I: Pesticide Residues Analysis

Evaluation of water exposure to pesticidesi h h i i i bwith emphasis on priority substances.

Development of analytical methodologies for ti id ith hi h t ti l t t i tpesticides with a high potential to contaminate

the ecosystem

Field Sampling Analysis

Pesticides routinely analysed by SPME / GCPesticides routinely analysed by SPME / GC--MS MS

alachloralachlor ethofumesateethofumesate

atrazineatrazine lindanelindane

propanilpropanil

3,43,4--dicloroanilinedicloroanilineatrazineatrazine lindanelindane

desethylatrazinedesethylatrazine metolaclormetolaclor

d i l t id i l t i

3,43,4 dicloroanilinedicloroaniline

propazinepropazine

i ii idesisopropylatrazinedesisopropylatrazinemetribuzinemetribuzine

ii li tli t

simazinesimazine

terbuthylazineterbuthylazinecyanazinecyanazine molinatemolinate

chlorfenvinphoschlorfenvinphos oxadiazon oxadiazon terbutrynterbutryn

trifluralintrifluralinchlorpyrifoschlorpyrifos pendimetalinpendimetalin

dichlobenildichlobenil pirimicarbpirimicarbαα--endosulfanendosulfan

ββ endosulfanendosulfanpp

dimethoatedimethoate prometrynprometrynββ--endosulfanendosulfan

Unit II: Toxic Effects EvaluationUnit II: Toxic Effects Evaluation

Determination of toxic effects in:• Water• Water

Daphnia magna Pseudokirchneriella subcapitata• Sediment / soil

Heterocypris incongruens

Determination of the EC50 for active ingredients and formulatedDetermination of the EC50 for active ingredients and formulated products

Field Sampling Biological Testing Effect EvaluationField Sampling Biological Testing Effect Evaluation

Formulated Product

AREAS OF RESEARCH ACTIVITIESAREAS OF RESEARCH ACTIVITIESE al ation of the en ironmental impact of pesticides on ater andEvaluation of the environmental impact of pesticides on water and

soil in agricultural ecosystems, based on:

1 Modelling for prediction of:1. Modelling for prediction of:• Fate and environmental behaviour of pesticides

(multicompartment models, leaching indices)P t ti l i k f ti id ( i t l i di t ) b d• Potential risk of pesticides (environmental indicators) based on their physicochemical properties and toxic effects on different organisms representative for environmental systems

2. Sampling of environmental matrices in the field

AREAS OF RESEARCH ACTIVITIESAREAS OF RESEARCH ACTIVITIESDevelopment of Decision Support Systems through an adequate

selection of pesticides and pollution risk mapping

Probability of groundwater contamination by pesticides

0 – 25 %

26 – 50 %

51 – 75 %%

76 – 100 %

By considering specific pesticide characteristics and local environmental conditions, this contributes to a support system for pesticide selection ensuring a sustainable use of pesticidespesticide selection ensuring a sustainable use of pesticides

ACADEMIC ACTIVITIESACADEMIC ACTIVITIES( )( ) ( )( ) ff SSInIn BachelorBachelor (B) (B) andand MasterMaster (M) (M) programsprograms ofof ISAISA

AgriculturalAgricultural EngineeringEngineering EnvironmentalEnvironmental EngineeringEngineeringPlantPlant ProtectionProtection (B)(B) En ironmentalEn ironmental Poll tionPoll tion (B)(B)-- PlantPlant ProtectionProtection (B)(B) -- EnvironmentalEnvironmental PollutionPollution (B)(B)

-- PesticidesPesticides andand EnvironmentEnvironment (M)(M) -- EnvironmentalEnvironmental MonitoringMonitoring (B)(B)-- IntegratedIntegrated PestPest ManagementManagement -- AppliedApplied ChemistryChemistry (M)(M)StrategiesStrategies (M) (M) -- AppliedApplied EcologyEcology andand NatureNature

-- CropCrop ProtectionProtection (M)(M) ConservationConservation (M)(M)

FoodFood ScienceScience andand EngineeringEngineeringFoodFood ScienceScience andand EngineeringEngineering-- NutritionNutrition, , FoodFood ContaminationContamination andand ToxicologyToxicology (M)(M)

Animal Animal ScienceScienceChemistryChemistry andand BiochemistryBiochemistry LaboratoryLaboratory (M)(M)-- ChemistryChemistry andand BiochemistryBiochemistry LaboratoryLaboratory (M)(M)

BiologyBiology-- General General BiologyBiology LaboratoryLaboratory (B)(B)

Academic and ProfessionalAcademic and Professional FormationFormation::Academic and ProfessionalAcademic and Professional InternshipsInternships-- Academic and Professional Academic and Professional InternshipsInternships

-- MastersMasters-- PhDsPhDs

R&D Activities R&D Activities -- Research ProjectsResearch Projects

Present studies at the Laboratório de Ecotoxicologia of

DPPF/ISA are integrated in the scope of national projects,

lik “Pl d i t ã d l i t dlike “Plano de intervenção e desenvolvimento de um

Sistema de Apoio à Decisão para

o Norte da Zona Aluvionar do Tejo”

(AGRO 530) and internacional projects like the Sixth(AGRO 530), and internacional projects, like the Sixth

FrameWork Programme Priority “Water Resource

Strategies and Drought Alleviation in Western Balkan

Agriculture” (WATERWEB).Agriculture (WATERWEB).

SERVICES TO THE COMMUNITYSERVICES TO THE COMMUNITYSERVICES TO THE COMMUNITYSERVICES TO THE COMMUNITY

Analysis of pesticide residues in waterAnalysis of toxic effects in water and soil/sediment

Consultancy in the selection of phytofarmaceutical products for various crops and environmental management of p p gpesticides

R&D studies in colaboration with institutes and companies of several sectors

Advanced training for senior technicians of institutes and companiesof institutes and companies

Divulgation program of ISA for secondary schools –“Do Laboratório de Ecotoxicologia ao Ecossistema” (“From Ecotoxicology Laboratory to the Ecosystem”)

EXTERNAL RELATIONSEXTERNAL RELATIONS

Universities Universities

Public institutionsPublic institutions

Regional Directorate of AgricultureRegional Directorate of Agriculture DRARO DRARO DRABL DRABL DRAALDRAALDGADRDGADRDGADRDGADR

TEAMTEAM

Maria José Cerejeira (Coordinator; mcerejeira@isa.utl.pt)j ( ; j @ p )

Michiel Daam (Research Scientist; mdaam@isa.utl.pt)

Emília Silva (PhD student; emiliasilva@isa utl pt)Emília Silva (PhD student; emiliasilva@isa.utl.pt)“Integrated approach on the risk assessment of realistic pesticide mixtures for water protection”

Sara Leitão (PhD student; saraleitao@isa.utl.pt)“New improvements on pesticide ecological risk assessment on the soil-water interface”

Instituto Superior de Agronomia UTLInstituto Superior de Agronomia - UTLDPPF/SAPI - Laboratório de EcotoxicologiaTapada da Ajuda1349-017 LisboaTEL: 21 365 3219/ 3224/ 3429/ 3222http://www.isa.utl.pt/home/node/820