Upload
newton
View
27
Download
0
Tags:
Embed Size (px)
DESCRIPTION
Instrumental Analysis, Laboratory Oral Presentation. Nikko Bacalzo Douglas Dy Jeremy Ly France Tan. Determination of Anions in Toothpaste by Ion Chromatography . Instrument Used: Ion Chromatography. FLUORIDE. http://toxicfreekidsblog.org/2011/07/toxic-toothpaste / - PowerPoint PPT Presentation
Citation preview
Instrumental Analysis, LaboratoryOral PresentationNikko BacalzoDouglas DyJeremy LyFrance Tan
Determination of Anions in Toothpaste by Ion Chromatography Instrument Used:Ion Chromatography
FLUORIDE
http://toxicfreekidsblog.org/2011/07/toxic-toothpaste/http://www.webmd.com/oral-health/image-collection-oral-health#phototake_rm_photo_of_tooth_illustration.jpg
http://www.skepticnorth.com/2010/10/fluoridiated-water-debate-returns-nothing-new-is-said/http://www.fluoridation.com/teeth.htm
ION CHROMATOGRAPHY
ION CHROMATOGRAPHY
http://www.separations.us.tosohbioscience.com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/IonExchange
ION CHROMATOGRAPHY
Injection valve
Readout
Mobile phase/ solvent reservoir Pump Column Detector
Excess/waste
Filter
Data and Results
Data and Results
0 5 10 15 20 25 30 350
50000
100000
150000
200000
250000
300000
350000
f(x) = 21381.584705256 x + 50726.6666666663R² = 0.892850575318569
concentration (ppm)
peak
are
a
Fluoride calibration
curve
Data and Results
0 5 10 15 20 25 30 35 40 450
50000
100000
150000
200000
250000
f(x) = 31566.7370857077 x − 30763.6666666669R² = 0.861303259627165
concentration (ppm)
peak
are
a
Chloride calibration
curve
Data and Results
BrandIon n
%(w/w) mean ± std dev
%RSD
Sensodyne
F 3 -- --
Cl 2 a0.00923 ± 0.00002
0.270
Colgate F 3 -- b --Cl 3 -- --
HapeeF 3 -- --Cl 2 a 0.016 ± 0.002 15.3
Close-Up
F 3 -- --Cl 3 -- --
Data and Resultsconc
(ppm)spiked (mg)
unspiked (mg)
added
(mg)
%recovery
SPIKE 40.62 2.031 0.02052 1.344 149.6
Conclusion• Four toothpaste brands were analyzed for their fluoride
and chloride contents (Sensodyne, Colgate, Close-Up and Hapee).
• Standards were run in the ion chromatograph, and the retention times and the concentration-peak area relationships of each were drawn from the data.
• Out of which only Sensodyne and Hapee showed quantifiable amounts of chloride in the toothpaste formulation.
• Calculation yielded concentrations of 0.00923% and 0.016% chloride in both toothpastes, respectively.
• Other samples had no sufficiently quantifiable amounts of both anions, suspected to be consequence of matrix effect.
Recommendations• Use other MP: carbonate/bicarbonate buffer, KOH• Use gradient elution• Standard addition instead of calibration curve• Homogenization of samples• Analyze other anions (MFP, benzoate, sulfate,
phosphate)• Method validation
References• Takahara, Y, et al. Photooxidation of organic
compounds in a solution containing hydrogen peroxide and TiO2 particles under visible light. Journal of Applied Chemistry. Vol.35, 793-797. <http://www.springerlink.com/content/t866330021785302/>
• Shimadzu Corporation. The UV-2401PC: A Superbly Engineered Instrument. Accessed on May 21, 2012. <http://www.pa.ibf.cnr.it/web/pdf/12.pdf>
Thank you for listening