Instrumental Analysis

CHE 403

Jinseok Heo

Alexander Nazarenko

Synthetic Chemistry Analytical Chemistry

To make a new stuff To find out what is it

“white powder”

4.2 Instrumentation. The characterization and analysis of chemical systems requires an appropriate suite of modern chemical instrumentation and specialized laboratory apparatus.• Instrumentation should be modern, high quality, and properly maintained.• Approved programs must have a functioning NMR spectrometer that undergraduates use in instruction and research. • Throughout their curriculum, undergraduates must use instrumentation from most of the broad categories listed below:Optical spectroscopy (e.g., UV-vis, FTIR, fluorescence, atomic absorption and emission, Raman, laser)Chromatography and separations (e.g., HPLC, GC, electrophoresis)Mass spectrometry (e.g., MS, GC-MS)Electrochemistry (e.g., potentiometry, voltammetry)Imaging and microscopy methods (e.g., electron microscopy, scanning probe microscopy)

From “Professional Education in Chemistry. Guidelines.”

Analytical chemistry sequence @ BSC

CHE111-112Titrations with NaOH, EDTA, KMnO4, K2Cr2O7, , I2

Some separations and photometry

CHE203-204 Identification of organic compounds; IR, NMR, GC

CHE301 Analytical Chemistry itself: gravimetry, titrations,electrochemistry and spectrophotometry

CHE403 Instruments, how they work and how to operate them

Biochemistry, Forensic Lab, Analytical Toxilcology, and Inorganic Chemistry

- More about analytical methods

Quantitative Chemical Analysis6th Edition and 7th Edition

Daniel C. Harris (Michelson Laboratory, China Lake)

A Chemical Analysis

1.Define the problem

2. Choose the method

3. Obtain the sample

1.Define the problem2.Choose the method3. Obtain the sample

Sample preparation (preconcentration, separation,removal of interferences)

Run assay (determination

Data Processing

Data Analysis and Interpretation

Garbage in,Garbage out!

Basic measures:

Metric System

Mole

Atomic mass (Atomic weight)

Formula mass (Formula weight)

Molecular mass ( Molecular weight)

Concentration:

Weight-to-weight (w/w)

Weight-to-volume (w/v)

Volume to volume (v/v)

Number-to-volume (molarity)