Instructions• Prepare 3 nos. of A3 size posters• The poster may run as follows

– Title– Author(s)– Introduction– Objective– Materials and Methods– Results– Conclusion/ Interpretation– Acknowledgements

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Impact analysis of microbial diversity and functional genes of soil influenced by farming systems

C. Chinna durai, D. Balachandar and G. GopalaswamyDept. of Agrl. Microbiology, TNAU, Coimbatore -3, India

Introduction Soil microorganisms are an important biological factor that decides the fertility of all kinds of soils.Organic manures and chemical fertilizers are used primarily to increase the nutrient availability to the plants, but they can also affect the soil microorganisms further leads to influence plant growth.Several short-term and long-term studies showed an increased microbial biomass and activity due to addition of organic amendments (Lagomarsino et al., 2009).In the present experiments, assessment of microbial changes and enzymatic activities of soil influenced by two different nutrient managements practiced for long-term was carried out.

Objectives To study the influence of nutritional management practices

on biological activities and fertility of the soil. To study the effect of long-term nutritional management on

eubacterial community and functional genes of soil. To identify the bacterial species and/or functional genes

which are adversely affected by management practices. To develop a probe (microbe or gene based) to monitor the

long-term fertility of soil.

Physio-chemical properties

Control soil100%

Organic soil100% NPK

soil

pH 8.59 ± 0.01 8.54 ± 0.02 8.50 ± 0.03

Ec (dSm-1) 0.35 ± 0.21 0.13 ± 0.01 0.18 ± 0.01

Texture Sandy clay loam (SCL)

Lime Calcareous (C)

N (kg/ha) 86.80 ± 5.83 83.07 ± 0.93 74.67 ± 1.87

P (kg/ha) 37.97 ± 2.09 36.50 ± 8.23 40.50 ± 3.98

K (kg/ha) 338.33 ± 8.84 431.00 ± 2.52 356.67 ± 5.21

Copper (µg/g) 1.19 ± 0.01 1.32 ± 0.09 1.34 ± 0.01

Manganese (µg/g)

5.81± 0.24 6.11 ± 0.38 5.67 ± 0.16

Iron (µg/g) 1.55 ± 0.05 1.00 ± 0.18 1.48 ± 0.12

Zinc (µg/g) 0.16 ± 0.01 0.78 ± 0.19 0.43 ± 0.04

Result

Physico-chemical properties of soil influenced by long-term nutrient managements

Materials and methodsSoil samples from three treatments of 100 years old Long-term experimental station were analyzed forMicrobial population (James .,1958)Enzyme assay (Weaver et al., 1994)

Culturable microbial populations of soil influenced by long-term nutrient managements

Summary The physio-chemical and nutrient status of the soil were not dramatically changed due to long-term influence of nutrient managements. The cultivable microbial populations present in OF soils were significantly higher than control and CF soils. The soil enzymes assayed for the treatments revealed that acid and alkaline phosphates, urease and dehydrogenase were significantly higher in OF soils than CF soils. The asparaginase activity was higher in CF soil than other two soils

References James, N. 1958. Can. J. Microbiol., 4:363-370.Lagomarsino,et al ., 2009 Annual Review of Phytopathology 10.3832/efor0588-006 Weaver et al., 1994. Methods of soil analysis

Asparaginase and urease activity of soil

Acid and alkaline phosphatase activity of soil Dehydrogenase activity of soil