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Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
PRINCIPLESANDAPPLICATIONSOFFLUORESCENCEMICROSCOPY
26 February – 9 March 2018
DIRECTORS
David DIGREGORIO Jost ENNINGA Gaël MONERON Institut Pasteur Institut Pasteur Institut Pasteur
HEAD OF LABORATORY
Florian RÜCKERL Institut Pasteur
Teaching Assistants
Laurent AUDRY Institut Pasteur
Mickael LELEK Institut Pasteur
Patricia LATOUR-LAMBERT
Institut Pasteur
Joana LOURENCO
ICM - INSERM
Nelson REBOLA ICM
Cécile SAINT CLOMENT
Institut Pasteur
Jean-Yves TINEVEZ Institut Pasteur
Julie NGUYEN CPN-INSERM
Diana ZALA CPN- INSERM
Marie-Pierre ADAM Institut Pasteur
Min Hee KIM Institut Pasteur
Olivier DISSON Institut Pasteur
Course Staff
Guylène K’OUAS Institut Pasteur
Gauthier PUIME Institut Pasteur
Hervé WAXIN Institut Pasteur
Location Education Center – Institut Pasteur – Pavillon Louis Martin
28, rue du Docteur Roux 75015 Paris Lectures: Room No 2; Practical Work: Room on the ground floor
With the participation of:
Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
WEEK1 Monday, 26 February 8:00 – 8:30 Badges 8:30 – 9:30 Welcome, Admin, and Introduction David DIGREGORIO
Jost ENNINGA Gaël MONERON
(Institut Pasteur, France) 9:30 – 12:00 Basic optics I Gaël MONERON
• Geometrical and Gaussian optics (Institut Pasteur, France) • Basic principles of a light microscope • Contrast techniques
13:30 – 18:30 Bench optics practicals 19:00 Welcome drink Tuesday, 27 February 9:00 – 12:00 Basic optics II Gaël MONERON
• Coherence of light (Institut Pasteur, France) • Diffraction, Fourier optics, Resolution • Objective lenses and aberrations
13:30 – 18:30 Bench optics practicals Wednesday, 28 February 9:00 – 12:00 Fluorescence, light sources Gaël MONERON
• Photo-physics (Institut Pasteur, France) • Multi-color imaging strategies • Lamps, LEDs, LASERs, wavelength selection devices
13:30 – 14:30 Imaging modalities, detectors David DIGREGORIO
• Wide field / beam Scanning (Institut Pasteur, France) • Cameras / point detectors • Signal-to-noise ratio (sources of noise) • Sampling (time and space)
14:30 – 18:30 Bench optics practicals
Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
Thursday, 1 March 9:00 – 9:45 Confocal laser scanning microscopy David DIGREGORIO
(Institut Pasteur, France) 9:45 – 10:30 Dynamic imaging David DIGREGORIO
(Institut Pasteur, France) 10:30 – 12:00 Multi-photon microscopy Florent HAISS
(Institut Pasteur, France) 13:30 – 14:00 Estimating resolution (PSF) David DIGREGORIO (Institut Pasteur, France) 14:00 – 17:00 PSFs practicals on commercial microscopes 17:00 – 18:30 Wrap-up Friday, 2 March 9:00 – 10:30 Biosensors Jost ENNINGA
(Institut Pasteur, France) 10:30 – 12:00 New fluorescent probes Marcel BRUCHEZ (Carnegie Mellon Univ., USA) 13:30 – 15:00 Structured illumination microscopy Alexandra FRAGOLA for enhanced resolution imaging (Univ. Pierre & Marie Curie, France) in biology 15:00 – 15:30 Imaging infection Jost ENNINGA (Institut Pasteur, France) 15:30 – 18:30 Fixed preparation imaging practicals
• Fluorescent neurons • Microtubules • Bacterial invasion
Saturday, 3 March 9:00 – 12:00 Introduction to image analysis Jean-Yves TINEVEZ for Life-Science microscopy (Institut Pasteur, France) 13:00 – 18:00 Image analysis practicals
• Image analysis • Data presentations
Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
WEEK2 Monday, 5 March 9:00 – 10:30 Single molecule localization Christophe ZIMMER microscopy principles (Institut Pasteur, France) 10:30 –12:00 TIRF and SAF microscopy Sandrine LEVEQUE-FORT
(Univ. Paris-Sud, France) 13:30 – 15:00 STED Microscopy Gaël MONERON (Institut Pasteur, France)
15:00 – 18:30 Fixed preparation imaging practicals • Fluorescent neurons • Microtubules • Bacterial invasion • Organelles
Tuesday, 6 March 9:00 – 10:30 FRAP/Photoactivation Jeremy DITTMAN
(Weill Cornell Medical College, USA) 10:30 – 12:00 FCS/FLIM Christian EGGELING
(Oxford University, UK) 13:30 – 15:00 Single molecule/FRET/TIRF Keith WENINGER
(North Carolina State, USA) 15:00 – 18:30 Live cell imaging practicals
• XFP neurons (culture, acute brain slices) • Live Bacteria invasion • Organelles
Wednesday, 7 March 9:00 – 10:30 High Content Screening Nathalie AULNER (Institut Pasteur, France) 10:30 – 12:00 Imaging the immune system Philippe BOUSSO (Institut Pasteur, France) 13:30 – 15:00 Light-sheet fluorescence microscopy Willy SUPATTO
(Ecole Polytechnique, France) 15:00 – 18:30 Live cell imaging practicals
• Calcium imaging (culture, acute brain slices) • Rac Photoswitching • FRAP, paGFP photoactivation
Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
Thursday, 8 March 9:00 – 10:30 Imaging deep in scattering tissue Sylvain GIGAN (Univ. Paris Pierre et Marie Curie, France) 10:30 – 12:00 Wrap-up and Analysis 13:30 – 18:30 Live cell imaging practicals
• Calcium imaging (culture, acute brain slices) • Rac Photoswitching • FRAP, paGFP photoactivation
Friday, 9 March 9:00 – 17:30 Analysis and final presentations 17:30 – 18:30 Written exam and course evaluation 19:00 Aperitif/Dinner/Social
Institut Pasteur Course: Principles and Applications of Fluorescence Microscopy 2017-2018
Administration Institut Pasteur Education Center - 28, rue du docteur Roux Building 9C - Pavillon Louis Martin Isabelle DI MARTINO Tél : +33 (0)1 45 68 82 89 EMAIL : [email protected] [email protected] Registrar’s Office 28, rue du docteur Roux Building 13 - Salle Saint-Joseph de Cluny Sylvie MALOT Tél : +33 (0) 45 68 81 41 EMAIL : [email protected]