Insect Diversity

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    INSECT DIVERSITY

    Project Report

    Reshma Soman IMS10046

    Group members

    Reshma Soman

    Sivaramakrishnan.S

    Sanjay Kumar Meena

    CONTENTS

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    1) INTRODUCTION

    2) AIM

    3) PROCEDURE

    4) ANALYSIS

    5) CONCLUSION

    6) DRAWBACKS

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    Introduction

    Our project involves sampling the diversity of insects usingvarious methods and comparing which method serves to be moreuseful for quantitative sampling of insect species.The methods we are going to use to study the diversity of insectsare:-

    1) Pitfall trap2) Pan trap3) Baited trap1) Pitfall Trap:-Pitfall traps consist of a container (tin, jar or drum) buried in theground with its rim at surface level used to trap mobile animalsthat fall into it. The fluid going to used in this trap isMethyl Parathion.2) Pan Trap:-The pan Trap Consists of small coloured pan or bowl filled with

    soapy water to catch the insects.3) Baited Trap:-It is same that of Pitfall Trap, Here we use Bait(some substance)to attract the insects instead of Alcohol.Field and weather

    Should not be disturbed by human or any animal.

    Vegetative environment is essential for better results.

    Humidity climate gives positive results in the experiment.

    Pollution free environment also gives positive results .

    (smoke, charcoal)

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    AIM

    Our aim is to study which methodology is adequate for sampling

    insects and differentiating habitats on the basis of insect species

    distribution

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    PROCEDURE

    We prepare the traps, and then we placed the traps where itseems to have favourable conditions for our aim. We collectedthe samples at various times or various conditions and wepreserved the specimens for Data recording and Analysis. In datarecording, we recorded the plot, Site and quadrate, informationon the order, family, serial number, number of nymphs or larvae

    and the number of adults which were observed and trapped withthe above said methods. In data analysis, we tried to understandthe distribution of species of insects over a particular place withvarying conditions.From above data analysis, we concluded about the Insect speciesdiversity within our local area.

    Pan Trap

    The most commonly deployed recent protocol is the use ofcolored pan traps (bowls) filled with soapy water. Insects

    approach the bowls, land on the water, and drown.The methodis particularly good at catching numerous species of bees, but

    can also be effective for capturing various flower-visiting flies

    and skippers.

    Pit fall

    Pit fall trap consists of 1-2 litre plastic jar with an opening of 9cm which is buried The insects trapped in pit fall are preserved in

    70% alcohol, at ground level and observed for 3-4 days.Each jarconsists of 50 ml of soap water.

    Baited Traps

    A plastic jar of 2.5 litre capacity was used to fabricate a scentedtrap. The trap was baited with 200 ml saturated jaggery

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    (unrefined cane sugar ) and Pineapple essence.The scented traps

    were also set between 1500 -1700 hrs and collected insects were

    filtered, washed and preserved in 70% alcohol.

    Collected insects were stored in 70% alcohol

    DATA COLLECTION

    Samples From Traps

    Trial 1 Trial 2 Baited Trap:-Butterflies-3Fruitflies-9Wasps-4Wingless Cockroach-5Pitfall Trap:-Mantis-6Wingless Cockroach-10

    Spiders-2Pan Trap:-

    Baited Trap:-

    Fruit flies-6

    Wasps-4

    Wingless Cockroach-1

    Pitfall trap:-

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    Wasps-2Fireflies-1Fruit flies-5Mantis-4ButterFlies-3

    Mantis-10

    Spiders-2

    Fruitflies-14

    WinglessCockroach-15

    Wasps-6

    Fireflies-1

    Total Individuals-51

    Mantis-8

    Brown Beetles-6

    Ant mimicking Spiders-3

    Wingless Cockroach-2

    Pan trap:-

    Mantis-6

    Spiders-2

    Fireflies-2

    Fruit flies-4

    Mantis-14

    Spiders-2

    FruitFlies-10

    Wingless Cockroach-3

    AntMimickingSpiders-3

    Wasps-4

    Fireflies-2

    Brown Beetles-6

    Total Individuals-44

    DATA RECORDED FROM SAMPLE

    Butterflies

    Mantis

    Spiders

    3

    24

    4

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    Fruit flies

    Wingless Cockroach

    Ant like spiders

    Wasps

    Fireflies

    Brown Beetles

    24

    18

    3

    10

    3

    6

    Total Individuals 95

    ANALYSIS

    Finding Diversity for total no. Of individuals

    Pb=3/95=0.031578947

    Pm=24/95=0.252631579Ps=4/95=0.042105263

    Pfr=24/95=0.252631579

    Pwc=18/95=0.189473684

    Pams=3/95=0.031578947

    Pw=10/95=0.105263158

    Pfi=3/95=0.031578947

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    Pbb=6/95=0.063157895

    D= 1 - {(0.031578947)^2 + (0.252631579)^2 + (0.042105263)^2

    +

    (0.252631579)^2 + (0.189473684)^2 + (0.031578947)^2 +(0.105263158)^2

    + (0.031578947)^2 + (0.063157895)^2}

    D=0.81662

    Efficiency of Methods

    Baited Trap:-Diversity from first sampling

    Total Individuals=(3+9+4+5+6+10+2+2+1+5+4)=51

    Pbb=0.0588235

    Pfr=0.176471

    Pw=0.0784314

    Pwc=0.0784314

    D=0.953095

    D (for individuals caught in this trap)=0.702948

    Baited Trap:-Diversity from second sampling

    Total Individuals=(6+4+1+8+6+3+2+6+2+2+4)=44

    Pfr=0.136364

    Pw=0.0909091

    Pwc=0.0227273

    D=0.972624

    D (for individuals caught in this trap)=0.561983

    Pitfall trap:- Diversity from first sampling

    Total Individuals=(3+9+4+5+6+10+2+2+1+5+4)=51

    Pm=0.117647

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    Pwc=0.196078

    Ps=0.0392157

    D=0.946175

    D(for individuals caught in this trap)=0.567901

    Pitfall trap:- Diversity from second sampling

    Total Individuals=(6+4+1+8+6+3+2+6+2+2+4)=44

    Pm=0.181818

    Pbb=0.136364

    Pans=0.0681818

    Pwc=0.0454545

    D=0.941632

    D(for individuals caught in this trap)=0.686981

    Pan Trap:-Diversity from first sampling

    Total Individuals=(3+9+4+5+6+10+2+2+1+5+4)=51

    Pw=0.0392157

    Pfi=0.0196078

    Pfr=0.0980392

    Pm=0.0784314

    D=0.982314

    D(for individuals caught in this trap)=0.680556

    Pan Trap:-Diversity from second sampling

    Total Individuals=(6+4+1+8+6+3+2+6+2+2+4)=44

    Pm=0.136364

    Ps=0.0454545

    Pfi=0.0454545

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    Pfr=0.0909091

    D=0.969008

    D(for individuals caught in this trap)=0.693878

    Insects Population Distribution

    The above graph was obtained from sampling of the insects, it

    represents how the population of the insects, could have been

    distributed over the area.The field where we had our traps andworked has insects, which depend upon same niche and the vegetation

    is same throughout the field.

    STATISTICAL APPROACH

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    CONCLUSION

    The average efficiency of the methods for getting insects of

    diversified species are as follows:-

    Baited =0.96

    Pitfall=0.94

    Pan=0.97

    Average of Diversity among insect species caught in traps are as

    follows:-

    Baited= 0.63

    Pitfall=0.62

    Pan=0.68

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    DRAWBACKS

    Time:-This One month cannot give whole idea about the field(or

    the habitat)where we set traps for sampling and the insect

    population distribution over that .Frequency of sampling was notefficient enough because of climate. Once in two weeks, which

    can provide only little bit of reliability, because more no. of

    sampling could have increased reliability of this result.

    REFERENCE

    1)insect species diversity in the tropics:

    Sampling methods and a case study

    Raghavendra gadagkar, k. Chandrashekara and padmini nair

    Journal of the bombay natural history society

    December 1990,Vol.87,No.3

    2)Comparison of Pan Trap and Intensive Net Sampling Techniques

    for

    Documenting a Bee (Hymenoptera: Apiformes):Fauna

    T'ai H. Roulston, Stephen A. Smith,1'3 and Amanda L. Brewster2

    University of Virginia, Blandy Experimental Farm, 400 Blandyarm

    Lane, Boyce,Virginia, 22620 USA

    JOURNAL OF THE KANSAS ENTOMOLOGICAL SOCIETY

    80(2), 2007, pp. 179-181