Innovative by NatureInnovative by NatureTMTM

Dr. E.N. Zyme Biology AP, Monta Vista High School – September 13, 2013

Disclaimer: This presentation is used as a SIMULATION activity for high school students. The story is purely fictitious and is made for students to understand the real world applications for enzymes. However, student data will be reported to Genencor for review.

Genencor TodayGenencor Today Proven performer since 1982Proven performer since 1982

Powerful, integrated technology –Powerful, integrated technology – i-biotechi-biotech

1500 Employees1500 Employees

Year 2000 - Public Offering Year 2000 - Public Offering

NYSE NYSE ‘‘GCORGCOR’’

Corporate Offices - Palo Alto, Rochester Corporate Offices - Palo Alto, Rochester

NY, Leiden NETH.NY, Leiden NETH.

Manufacturing - Argentina, US, Finland, Belgium, Manufacturing - Argentina, US, Finland, Belgium,

ChinaChina

GenencorGenencor’’s Visions Vision

Leverage Leverage i-biotechi-biotech platforms platforms into multi-billion dollar opportunitiesinto multi-billion dollar opportunities

Expand presence in our target marketsExpand presence in our target markets– Health CareHealth Care

– Agriculture Agriculture

– Industrial/consumerIndustrial/consumer

i-biotech i-biotech – efficient discovery and delivery – efficient discovery and delivery of safe and effective biomaterialsof safe and effective biomaterials

i-biotechi-biotech in Everyday Life in Everyday Life

$300+ million revenues, $16+ million net income, 44% gross margins $300+ million revenues, $16+ million net income, 44% gross margins

Cleaning Products - Cleaning Products - Clothes/DishesClothes/Dishes

ProteasesProteases– Removes protein stains (blood, grass, milk, Removes protein stains (blood, grass, milk,

tomato sauce)tomato sauce)

AmylasesAmylases– Removes starch stains (chocolate, gravy, Removes starch stains (chocolate, gravy,

rice, pasta)rice, pasta)

Why?Why?– Cleaner clothes/dishes, less chemicals, Cleaner clothes/dishes, less chemicals,

lower temperatures, enzymes are lower temperatures, enzymes are biodegradablebiodegradable

TextilesTextiles BiofinishingBiofinishing

– Cellulases for stonewashing and soft khakisCellulases for stonewashing and soft khakis

DesizingDesizing– Removal of starch using amylase during Removal of starch using amylase during

productionproduction

Why?Why?– Cellulases are less harsh on fabric, no Cellulases are less harsh on fabric, no

pumice stonespumice stones

– Starch initially protects textiles from Starch initially protects textiles from breakage. Amylases are an easy way to breakage. Amylases are an easy way to remove them so that the fabric can be remove them so that the fabric can be further treated. further treated.

Personal CarePersonal Care

Skin careSkin care– Enzymes can dramatically improve skin Enzymes can dramatically improve skin

softnesssoftness

Oral careOral care– Enzymes can remove plaqueEnzymes can remove plaque

Hair careHair care– Enzymes can remove dandruffEnzymes can remove dandruff

LowGenLowGen™ enzymes will open new markets™ enzymes will open new markets

AgricultureAgriculture Biomass into ethanolBiomass into ethanol

– Use enzymes to convert starch into sugars Use enzymes to convert starch into sugars that can be fermented into ethanol by yeastthat can be fermented into ethanol by yeast

Animal feed and pet foodAnimal feed and pet food– Enzymes can improve digestibility by Enzymes can improve digestibility by

increasing solubility of fibers and proteinsincreasing solubility of fibers and proteins

Production of high fructose corn syrupProduction of high fructose corn syrup– Enzymes can convert starch into high Enzymes can convert starch into high

fructose corn syrup (sweetener)fructose corn syrup (sweetener)

OtherOther Corn into ascorbic acid (Vitamin C)Corn into ascorbic acid (Vitamin C)

Fruit juicesFruit juices

Paper recycling (cellulases)Paper recycling (cellulases)

Your research focus...Your research focus... Optisize COOL

– Amylase enzymeAmylase enzyme

– Genencor Textile Finishing EnzymeGenencor Textile Finishing Enzyme

Possible uses on fabric:Possible uses on fabric:– Desizing Desizing

• Removes starch from fabrics Removes starch from fabrics

Your task…Your task… Test Optisize COOL at a broad ranges of Test Optisize COOL at a broad ranges of

temperatures and pHtemperatures and pH’’s to determine its usefulness. s to determine its usefulness. – Customers will want to know effectiveness of enzyme at Customers will want to know effectiveness of enzyme at

various pHvarious pH’’s and temperaturess and temperatures

Now…review the lab procedure!Now…review the lab procedure!

Overview of ProcedureOverview of Procedure Amylazyme Tablet Amylazyme Tablet

– Substrate for the experimentSubstrate for the experiment

– Blue dye crosslinked with amylose (starch) Blue dye crosslinked with amylose (starch)

How will you know how well the enzyme is working? How will you know how well the enzyme is working? – The more dye released = more enzyme activity The more dye released = more enzyme activity

Buffers (with enzyme) at 6 different pHs and 7 Buffers (with enzyme) at 6 different pHs and 7 temperatures will be tested (equilibrate to get it to the temperatures will be tested (equilibrate to get it to the right temperature)right temperature)

Each reaction will run for exactly 10 minutes Each reaction will run for exactly 10 minutes – Stop reaction after 10 minutes by adding Stop Solution Stop reaction after 10 minutes by adding Stop Solution

(Trizma Base)(Trizma Base)

– This denatures the enzymeThis denatures the enzyme

Overview of the ProcedureOverview of the Procedure Why filter?Why filter?

– Gets rid of the chunks of tablets that did not get Gets rid of the chunks of tablets that did not get reacted/broken downreacted/broken down

How to measure How to measure ““how bluehow blue”” it is? it is?– Spec20 Spec20

– Absorbance = how much light is being absorbed by the dye in Absorbance = how much light is being absorbed by the dye in the solution the solution

– Higher absorbance = higher enzyme activityHigher absorbance = higher enzyme activity