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Spider silk proteinsBasis for new materials

Thomas Scheibel

Lehrstuhl Biomaterialien

Universitt Bayreuth

www.fiberlab.de

Outstanding properties of spider silks are well known for thousands of years

high tensile strength

high elasticity

no inflammation

no allergic response

Spider silk a protein fiber


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Spider silk a diverse biopolymer

Rmer and Scheibel, Chem. Unserer Zeit2007, 41, 306Vendrely and Scheibel, Macromol. Biosci. 2007, 7, 401Heim et al.,Angew. Chem. Int. ed. 2009, 48, 3584

Spider silk fibers: mechanical properties


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- vascular wound repair devices

- haemostatic dressings and sutures

- drug delivery systems

- cosmetical products

- technical textiles / protective clothing

- surface / fiber coatings

- etc., etc., etc.

Possible applications for silk-based materials

availabilityofthenaturalproteins(e.g.cannibalismofspiders)

organizationofgenes

solution

analysisofproteincomponents

reversetranslationandgene designformodernbiotechnology

problems

Vendrely and Scheibel, Macromol. Biosci. 2007, 7, 401

Employing silk proteins for analysis


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Processing technology

Huemmerich et al., Biochemistry2004, 43, 13604Vendrely and Scheibel, Macromol. Biosci. 2007, 7, 401

can be biotechno-logically mimicked

Natural processing of spider silk proteins

Heim et al.,Angew. Chem. Int. ed. 2009, 48, 3584

What Spiderman should know


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Analysis of laminar flow and shear forces

Silk assembly in designed microfluidic device

10 m

simultaneous phase separation, pH-change and elongational flow

mixing andlaminar flow

analysis

Rammensee et a., PNAS 2008, 105, 6590

SEM

Possibleapplicationsintechnicaltextiles,

vascularwoundrepairdevices,andsutures.

Biomimetic spinning of spider silk proteins

Rmer and Scheibel, Bionik: Patente aus der Natur,2007, 130


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from nature

to new materials

Hardy et al., Polymer2008, 49, 4309

Advanced processing of spider silk proteins

Spie et al., GIT2007, 11, 928

strengthO2 permeability

cm1m/m/d (23C)

H2O permeability

85% humidity

g1m/m/d (23C)

silk film 20 MPa 18500 17500

PE 24 MPa 55000 30

PP 33 MPa 70000 55

PET 74 MPa1400

200

Films made of biotech silk


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Microcapsules made of biotech silk

emulsion in toluene

Hermannson et al,Adv. Mater. 2007, 19, 1810Hermannson et al, Phys. Chem. Chem. Phys. 2007, 9, 6442



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10 m

emulsion in toluene transfer into water


Hermannson et al,Adv. Mater. 2007, 19, 1810Hermannson et al, Phys. Chem. Chem. Phys. 2007, 9, 6442

Release is triggered by the addition of Proteinase K

Controlled release of encapsulate

light microscopy

fluorescencemicroscopy

Hermannson et al,Adv. Mater. 2007, 19, 1810


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Microspheres made of biotech silk

Lammel et al, ChemSusChem. 2008, 1, 413Slotta et al,Angew. Chem. Int. Ed. 2008, 47, 4592

Spider silk microspheres can be used for protein and drugencapsulation.

Spider silk microsphere formation


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Zeit[min]

drugrelease

Drug encapsulation in silk microspheres

Spidersilkmicrospherescanbeusedforencapsulatingpoorlywatersolublesubstances.

Liebmann et al., Colloids and Surfaces A: Physicochem. Eng. Aspects, 2008, 331, 126

E-carotene release in intestinalfluids

noE-carotene release in gastric fluids

in collaboration with

former Lab membersChristian Ackerschott

Simone HessDaniel Hmmerich

Lin RmerSayam SenGupta

Charlotte Vendrely

TUMHorst Kessler

Andreas Bausch

LMUGerhard Winter

Martin-Luther-Universitt HalleRainer Rudolph

Universitt Sussex, UKLouise Serpell

BayerischesStaatsministeriumfr

Umwelt,GesundheitundVerbraucherschutz

and further supporting companies

Acknowledgement


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Fibrous proteins for new applications

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