Initiation of DNA Replication in the Human Genome

Initiation of DNA Replication in the Human GenomeM.S. Valenzuela2014 February 06

OutlineIntroductionInitiation of eukaryotic DNA replicationActivation of origins of DNA replicationTemporal order of origin activationOrigin specification in eukaryotesMethods to detect replication origins in a genome-wide scaleGenomic distribution of human replication originsInitiation of DNA replication and cancerSummary and PerspectivesReferences

Methods to detect replication origins in a genome-wide scale

Chromatin immunoprecipitation (ChIP)PCR amplification of selected targets, hybridization to selected DNA microarrays, or deep DNA sequencingIdentify newly synthesized DNAnascent-strand abundanceDNA combingFluorescent in hybridization (FISH)