Powerpoint presentation for NCBI journal article reported in class
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Initiation of DNA Replication in the Human Genome
Initiation of DNA Replication in the Human GenomeM.S.
Valenzuela2014 February 06
OutlineIntroductionInitiation of eukaryotic DNA
replicationActivation of origins of DNA replicationTemporal order
of origin activationOrigin specification in eukaryotesMethods to
detect replication origins in a genome-wide scaleGenomic
distribution of human replication originsInitiation of DNA
replication and cancerSummary and PerspectivesReferences
Methods to detect replication origins in a genome-wide scale
Chromatin immunoprecipitation (ChIP)PCR amplification of
selected targets, hybridization to selected DNA microarrays, or
deep DNA sequencingIdentify newly synthesized DNAnascent-strand
abundanceDNA combingFluorescent in hybridization (FISH)
