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Asian Journal of Pharmaceutical ResearchYear : 2011, Volume : 1, Issue : 4First page : ( 119) Last page : ( 125) Print ISSN : 2231-5683. Online ISSN : 2231-5691.

Rapid RP-HPLC Method for Simultaneous Estimation ofSparfloxacin, Gatifloxacin, Metronidazole and Tinidazole

Sebaiy Mahmoud M.1,*, El-Shanawany Abdullah A.1, El-Adl Sobhy M.1, Abdel-Aziz LobnaM.1, Hashem Hisham A.2

1Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Egypt

2Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Egypt

*Corresponding Author E-mail: sebaiy_pharma@yahoo.com

Online published on 19 June, 2014.

AbstractAn isocratic RP-HPLC method had been developed for rapid simultaneous separation and determination ofsparfloxacin, gatifloxacin, metronidazole and tinidazole in pure form or in presence of some impurities within 5minutes. Separation was carried out on a Chromolith® Performance RP-18e (100 x 4.6 mm) using a mobile phaseof MeOH: 0.025M KH2PO4 adjusted to pH 3 using ortho -phosphoric acid (20:80, v/v) at ambient temperature. Theflow rate was 4 ml/min and maximum absorption was measured at 290 nm. The standard curve was linear in the

 

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10/4/2015 Indian Journals

http://www.indianjournals.com/ijor.aspx?target=ijor:ajpr&volume=1&issue=4&article=008 2/2

concentration range of 1–80 μg/mL for all drugs. The retention time of sparfloxacin, gatifloxacin, metronidazole andtinidazole was noted to be 4.3, 3, 1.8 and 1.2 minutes respectively, indicating shorter analysis time. The methodwas validated according to ICH guidelines. The proposed method was found to be accurate, reproducible, andconsistent. It was successfully applied for the analysis of these drugs in marketed formulations and could beeffectively used for the routine analysis of formulations containing any one of the above drugs, or a combination,without any alteration in the chromatographic conditions.

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KeywordsRP-HPLC, Sparfloxacin, Gatifloxacin, Metronidazole, Tinidazole.

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