INDEXADO NO / INDEXED IN CAB International/ Index ... Ingles SBTE-2004 - Programa .pdf · José Buratini Jr. (2 o Secretário) Cezinande de Meira (1 o Tesoureiro) Flavio Meireles

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INDEXADO NO / INDEXED INCAB International/ Index Veterinarius/ Veterinary Bulletin

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REVISTA ACTA SCIENTIAE VETERINARIAE

Faculdade de Veterinária - UFRGSCaixa Postal 15017 Fone: + (51) 3316 6118Av. Bento Gonçalves, 9500 Fax: + (51) 3316 7305e.mail: [email protected] (Provisório)C.E.P. 91501-970 - Porto Alegre, RS - Brasil

Capa: Laerte Ferreiro [Projeto Gráfico]; Cristiano da Rosa Pereira [Ilustração] & SamuelPlentz [Arte Final]

Editoração Eletrônica: Eliana Franco LopesWeb Designer: Itabajara da Silva Vaz Jr.

CIP - CATALOGAÇÃO NA PUBLICAÇÃO

Acta Scientiae Veterinariae - Vol 32 (Suplemento) - Porto Alegre: UFRGS - 2004

ISSN 1678-0345Nota: Continuação dos Arquivos da Faculdade de Veterinária UFRGS.Quadrimestral1. Medicina Veterinária - Periódicos I. Universidade Federal do Rio Grande do Sul/ Faculdade de Veterinária

CDU 619(05)

Ficha catalográfica elaborada por Rosaura Denise Ribas Moreira, CRB - 10/1231Biblioteca da Faculdade de Veterinária da UFRGS

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UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL

REITORAWRANA MARIA PANIZZI

VICE-REITORJOSÉ CARLOS FERRAZ HENNEMANN

PRO - REITOR DE PESQUISACARLOS ALEXANDRE NETTO

VICE - PRÓ - REITORA DE PESQUISAMARININHA ARANHA ROCHA

FACULDADE DE VETERINÁRIADIRETOR

ANTONIO DE PÁDUA FERREIRA DA SILVA FILHOVICE-DIRETOR

VLADIMIR PINHEIRO DO NASCIMENTO

CONSELHO EDITORIAL / EDITORIAL BOARDCarlos Marcos Barcellos de Oliveira

David Emilio Santos Neves de BarcellosDavid Driemeier

Fernando Pandolfo BortolozzoJosé Luiz Rodrigues

Laerte Ferreiro (Editor)Marisa Ribeiro de Itapema Cardoso

Rodrigo Costa MattosVladimir Pinheiro do Nascimento

COMITÊ CIENTÍFICO / SCIENTIFIC ADVISORY BOARDA. Wolf - UNESP (Botucatu)A. Barreto - Veterinário Autônomo (Brasília)A.C. Basso - USP (São Paulo)A. de S. Traldi - USP (Pirassununga)C.A. de M. Bonfim - UNESP (Araçatuba)C.R. Esper - UNESP (Jaboticabal)C. Meira - UNESP (Botucatu)C.R. Ferreira - UNESP (Jaboticabal)C.M. Barros - UNESP (Botucatu)C.L.V. Leal - USP (Pirassununga)E.G. Lo Turco - UNESP (Jaboticabal)F. Perecin - UNESP (Jaboticabal)F.C. Landim-Alvarenga - UNESP (Botucatu)F.V. Meirelles - USP (Pirassununga)G.Z. Mingoti - UNESP (Araçatuba)G. de P. Nogueira - UNESP (Araçatuba)J.C. Ferreira - UNESP (Botucatu)J.M. Garcia - UNESP (Jaboticabal)J.A. Visintin - USP (São Paulo)J. Buratini Jr. - UNESP (Botucatu)J.F. Garcia - UNESP (Araçatuba)J.L.M. Vasconcelos - UNESP (Botucatu)J.L. Rodrigues - UFRGS (Porto Alegre)J.M.B. Duarte - UNESP (Jaboticabal)

J.R. Figueiredo - UECE (Fortaleza)K.B. Avelino - UNESP (Jaboticabal)L.A.G. Nogueira - UFF (Niterói)L.S. de S. Barreto - UNESP (Jaboticabal)L. de A. Coelho - USP (Pirassununga)M.I.B. Rubim - UFSM ( Santa Maria)M.M. Seneda - UEL (Londrina)M. Roncoletta - UNESP (Jaboticabal)M.A. Alvarenga - UNESP (Botucatu)M.A.L. de Oliveira - UFRPE (Recife)M.A.N. Dode - EMBRAPA (Brasília)M.A. Miglino - USP (São Paulo)M.C.S. Caldas - UENF (Campos dos Goytacazes)M.D. Lopes - UNESP (Botucatu)M.B. Binelli - USP (Pirassununga)O.M. Ohashi - UFPA (Belém)P.B.D. Gonçalves - UFSM (Santa Maria)P.S. Baruselli - USP (São Paulo)R. Rumpf - EMBRAPA (Brasília)R.P. Arruda - USP (São Paulo)S.H. Gabaldi - Usp (São Paulo)S.C. Méo - UNESP (Jaboticabal)S.D. Bicudo - UNESP (Botucatu)V.F.M. Hossepian de Lima - UNESP (Jaboticabal)W. Yamazaki - UNESP (Jaboticabal)

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PRESIDENT’S LETTER

The annual meeting of the Brazilian Embryo Technology Society (SBTE) is characterized forthe combination of science and pleasure. Is a moment of integration between practitioners, industryand scientific community. The meeting has been showed to be highly efficient on the diffusion ofrecent knowledge’s and concepts on Animal Reproduction, more specifically on Embryo Technology.

Our Society is big today in several aspects: we are today the Brazilian Society with the largestnumber of Veterinarians (500) and our meeting the second of the world on Embryo Technology areaon number of papers and participants. Our size and quality is a natural consequence of the excellentwork of all SBTE past boards.

The SBTE members have been promoting a revolution on the application of Biotechnologies atfield conditions. We are today a standard on large scale utilization of Bovine and Equine EmbryoTransfer and also the first country of the world on production and transfer of IVF embryos.

I would like on this opportunity to thank all SBTE board members for their support. SpeciallyJoao Henrique and Meira for their work with the society newspaper and treasure, respectively. I wouldlike also to thank Fernanda, our Scientific Director, for her excellent job and dedication with theselection and publication of the lectures and abstracts.

We also want to congratulate all private and governmental sponsors. Your support and recognizingis fruit of the credibility that was build during the 19 years of SBTE.

As usual the official program aimed to balance based and applied aspects of embryo technology.We decided to maintain the pre conference short courses, and are including by the first time, workshopson specific areas.

Brazilian researches that are working in important laboratories from other countries were invited,for sure they are preoccupied in to adapt their acquired knowledge with our reality. The foreign speakersare from Argentina, country with which we have a lot to do together.

Dear Colleagues, the Society is yours and the event to you, we tried to do our best.

Be happy and welcome to Barra Bonita!

Marco Antonio AlvarengaPresident 2004 -2005

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ORGANIZING COMMITTEE OF THE XVIII ANNUAL CONFERENCE OF THE SBTEAugust 26 to 29, 2004

EXECUTIVE BOARDMarco Antonio Alvarenga (Presidente)

João Henrique Moreira Viana (Vice-Presidente)Carlos Antonio Carvalho Fernandes (1o Secretário)

José Buratini Jr. (2o Secretário)Cezinande de Meira (1o Tesoureiro)

Flavio Meireles (2o Tesoureiro)

SCIENTIFIC BOARD

EDITORS OF THE PROCEEDINGS:

Fernanda da Cruz Landim e AlvarengaMarco Antonio Alvarenga

DESKTOP PUBLISHINGJosé Luis Barbosa de Souza

Fernanda da Cruz Landim e Alvarenga (Presidente)Alberto GusmãoAlceu MezzaliraAlicio Martins Jr.Anneliese de Souza TraldiCarlos Eurico dos Santos FernandesCarlos Antonio Carvalho FernandesCesar R. EsperCezinande de MeiraCiro Moraes BarrosEduardo Paulino da CostaElen Silvia C. Siqueira-PylesFabiana Ferreira de SouzaFrederico Ozanan PapaGustavo F. CarneiroJoão Carlos Pinheiro FerreiraJoão Henrique Moreira VianaJoaquim Mansano GarciaJosé Antonio Visintin

José Buratini Jr.José Luis M. VasconcelosJosé Ricardo FigueiredoMara Iolanda B. RubinMarcelo NogueiraMarcelo M. SenedaMarco Antonio AlvarengaMarco Antonio Coutinho da SilvaMaria Angélica MiglinoMaria Denise LopesMario BinelliNereu C. PrestesPéricles A. HassunPietro S. BaruselliRoberto SartoriRodolfo RumpfRubens Paes de ArrudaSony Dimas BicudoVera F. M. Hossepian de Lima

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A SBTE AGRADECE O APOIO RECEBIDO:

DAS SEGUINTES INSTITUIÇÕES,

FINEPCNPq

FAPESPCAPESUNESP

DAS EMPRESAS DO CONDOMÍNIO DA SBTE

BIOCOM INDÚSTRIA COMÉRCIO E SERVIÇOS LTDA.BIOGENESIS DO BRASIL LTDA.

COOPERS DO BRASIL LTDA.EPPENDORF DO BRASIL LTDA.

FORT DODGE SAÚDE ANIMAL LTDA.GENEALÓGICA LTDA.

KLONEMBRYO - BIOTEC DA REPROD ANIMALLABORATÓRIOS CALIER DO BRASIL LTDA.

LABORATÓRIOS PFIZER LTDA.MINITUB DO BRASIL LTDA.

NUTRICELL NUTRIENTES CELULARES LTDA.SPECTRUN BIO ENG MED HOSPITALAR LTDA.

TECNOPEC LTDA.VITROGEN PESQ. DESENV. BIOTEC. DA REP. SC LT

E DOS DEMAIS EXPOSITORESAKZO NOBEL LTDA -DIVISÃO INTERVET

CONTROLMAX - CONSULTORIA E SISTEMAS LTDA.CULTILAB MATER P/ CULT DE CÉLULAS LTDA.

IVP - Instrum Veterinários e Palhetas do Brasil Ltda.PETS , Inc

SANTA LYDIA LTDA.TECNAL EQUIPAMENTOS PARA LABORATÓRIO LTDA.

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PROGRAM SBTE 2003

I. PRE-CONGRESS THEORETICAL COURSESAugust 26, 2004 (2:00 ás 6:00 pm)

1. The use of ultrasonography in Bovine Embryo Transfer ProgramsCarlos Antonio Fernandes – Biotran, AlfenasJoão H. Moreira Viana – Embrapa, Coronel Pacheco

2. Hormone-therapy in Equine Embryo Transfer ProgramsKaren Regina Perez – FMVZ – UNESP, BotucatuBeatriz Bringel – UFRRJ, Rio de Janeiro.

3. The use and limitations of Artificial Insemination in Small RuminantsSony Dimas Bicudo – FMVZ – UNESP, BotucatuJéferson Ferreira da Fonseca – EmbrapaYves Cognié – INRA, França

4. The use of Biotechnology in Small animals ReproductionMaria Denise Lopes – FMVZ – UNESP, BotucatuFabiana Ferreira de Souza – FMVZ – UNESP, Botucatu

II. ANUAL MEETING OF THE SBTE

August 26 20041:00 to 6:00 pm - Registration6:30 to 8:30 pm - Opening Session

Gabriel Bó – President of the IETS:Situation and perspectives of embryo transfer around the Word.

8:30 pm Opening Dinner

August 27 20048:00 to 9:00 am - Gabriel A. Bó - Animal Reproduction Istitute, Córdoba University, Argentina:

Hormonal manipulation of the estrous cycle in bovine embryo donors andrecipients

9:00 to 10:00 pm - Ciro Moraes de Barros – Department of Pharmacology, IB, UNESP, Botucatu,Brazil:Recent advances in hormonal treatments used for fixed-time artificial insemination(FTAI) in beef cattle

10:00 to 10:30 am - Coffee Break10:30 to 11:30 am - Roberto Sartori Filho- Embrapa Genetic Resources and Biotechnology, Brasília,

DF, Brazil;Fertilization and Embryonic Mortality in Cattle

11: 30 to 12:30 pm -Oral Presentation – Session I12:30 to 02:00 pm -Lunch2:00 to 3:30 pm - Students Competition3:30 to 4:30 pm - Andréa Giannotti – Human Reproduction Laboratory – College of Medicine

from ABC, Brazil:Quality control guidelines for in vitro embryo production laboratories

Acta Scientiae Veterinariae 32(Suplement), 2004

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4:30 to 5:00 pm - Coffee Break5:00 to 6:30 pm - Poster’s Session A

August 28 20048:00 to 9:30 am - Oral Presentation – Session II9:30 to 10:30 am - Marco Coutinho da Silva – ARBL - Colorado State University – USA:

Oocyte Transfer and Intracytoplasmic Sperm Injection in Horses10:30 to 11:00 am - Coffee Break11:00 to 12:30 pm - Mariano Medina - Goyaike SAACIYF Reproduction Center -Argentina:

Sorted semen application in productive animals12:30 to 2:00 pm - Lunch2:00 to 4:00 pm - Work Shops

1 – Research in IVP and cloning2 – Practical aspects of ET in Bovine3 – Practical aspects of ET in Equine4 – Practical aspects of ET in Small Ruminants

4:00 to 4:30 pm - Coffee Break4:30 to 6:00 pm - Poster’s Session B6:00 to 8:00 pm - SBTE Associate’s Assembly9:00 pm Closing Dinner and Students Competition Results

August 29 20048:30 to 9:30 am - Angélica Miglino – Department of Anatomy - FMVZ, USP, São Paulo, Brazil

Animal cloning and placentation9:30 to 10: 00 pm - Coffee Break10:00 to 11:30 pm - Round Table Discussion: Cryopreservation of oocytes and IVP embryos

Yeda Watanabe – USP –PirassunungaAlceu Mezalira - UESC-LagesArnando D. Vieira – UFRGS – Porto Alegre

11:30 to 12:00 pm - Closing Session

Work Shops

01) - Research in IVP and cloning in Brazil

Joaquim Manzano Garcia – FCAVJ – UNESP, JaboticabalRodolfo Rumpf – EMBRAPA - BrasiliaJosé Antonio Visintin – FMVZ – USP, São Paulo

02) - Practical aspects of ET in the Bovine

Luiz Fernando Nasser – USP - SPLuis Phelipe Cartolano Porto – Bauru, SPMarcio Marques USP - SP


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03) - Practical aspects of ET in the Equine

Perla Fleury – São José do Rio Pardo, SPCarlos Silveira Junior – Três Rios, RJJoão Batista Figueiredo Neto – São João da Boa Vista, SP

04) – Practical aspects of ET in small ruminants

César Nunez – PRAlberto Gusmão – UFBA, Salvador, BAJeferson Ferreira da Fonseca – Embrapa

Oral Presentation

Session IAugust 27 2004 – 11:30 to 12:30 pm

11:30 – 11:45: INTERFERON-TAU SECRETION IN CRYOPRESERVED IN VITRO PRODUCEDBOVINE EMBRYO. Araujo, M.C.C.; Ferreira, A.M.; Sá, W.F.; Barreto Filho, J.B.;Serapião, R. V.; Camargo, L.S.A.; Silva, M.V.G.B., Vale Filho, V.R.

11:45 – 12:00: INCREASED CONCEPTION RATE IN BOVINE EMBRYO RECIPIENTS WITHHIGHER PLASMATIC PROGESTERONE CONCENTRATION ON DAY OFEMBRYO TRANSFER. Reis, E.L.; Marques, M.O.; Carvalho, N.A.T.; Nasser, L.F.;Costa Neto, W.P.; Baruselli, P.S.

12:00 – 12:15: USE OF LONG ACTING PROGESTERONE FOR PREPARATION OF NON-CYCLIC EMBRYO RECIPIENT MARES Rocha Filho A.N.; Pessôa M.A.; GiosoM.M.; Alvarenga M.A.

12:15 – 12:30: EVALUATION OF THE TRANSCERVICAL EMBRYO RECOVERY IN SANTAINES EWES Silva, J.C.; Quintela, A.; Andrade Moura, J.C.; Resende, J.; Gordiano,H.; Martins, L.P.; Chalhoub, M.; Ribeiro Filho, A.L.; Gusmão, A.L.

Session IIAugust 28 2004 – 8:00 to 9:30 am

8:00 – 8:15: CRYOPRESERVATRION AND SHORT-TERM CULTURE OF ISOLATEDCAPRINE PRIMORDIAL FOLLICLES Rodrigues, A.P.R.; Costa, S.H.F.; Santos, R.R.;Lucci, C.M.; Nunes, J.F.; Ohashi, O.M.; Rondina, D.; Figueiredo, J.R.

8:00 – 8:30: DEVELOPMENTAL POTENTIAL OF BOVINE EMBRYOS FOLLOWING INVITRO OOCYTE FERTILIZATION WITH CATALASE AND GnRH Martins Júnior,A; Calegari, R.S.; Freitas, C.P.; Zanon, J.E.O.; Paschoal. D.M.


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8:30 – 8:45: EFFECT OF RETINOIDS AND IGF-I ON THE IN VITRO BOVINE EMBRYONICDEVELOPMENT Oliveira, M.A.L.; Lima, P.F.; Reichenbach, H.D.; Weppert, M.;Cavalcanti Neto, C.C.; Pina, V.M.R.; Santos, M.H.B.; Lima-Verde, I.B.

8:45 – 9:00: UTILIZATION OF A PORTABLE INCUBATOR (LIFETM) FOR IN VITROPRODUCTION OF BOVINE EMBRYOS . Siqueira-Pyles, E.S.C.; Pedrazzi, C.A.F.;Landim-Alvarenga, F.C.

9:00 – 9:15: EMBRYO PRODUCTION IN NELORE COWS USING IVF ASSOCIATED WITHET. Nonato Jr., I.; Rufino, F.A.; Sanches, B.V.; Pontes, J.H.F.; Uvo, S.; Ereno Jr., J.C.;Seneda, M.M.

9:15 – 10:00: EVALUATION OF EMBRYO SPLITTING AS AN ALTERNATIVE TO IMPROVEGESTATION INDICES IN EMBRYO TRANSFER HORSES. Silveira, L.L.; Souza,R.V.; Pimentel, C.M.; Rumpf, R.

Students Competition

August 27 2004 – 2:00 to 3:30 pm

14:00 – 14:15: PUBERTY INDUCTION AND SYNCHRONIZATION OF THE ESTRUS WITHPROGESTERONE, ESTRADIOL BENZOATE, GNRH AND ECG IN PREPUBERALHEIFERS OF THE NELORE BREED. Tetzner, T.A.D.; Diniz, E.G.; Jacomini, J.O.;Peres, R.F.G.; Amorim, L.L.

14:15 – 14:30: OPTIMIZING THE NUMBER OF RECIPIENT COWS SYNCHRONIZED WITHOVSYNCH PROTOCOL. Beltrame, R.T.; Barioni, L.G.; Veloso, R.V.; Saueressig,M.G.

14:30 – 14:45: CITOPLASMATIC MORFOLOGY EVALUATION OF EQUINE OOCYTESMATURED IN DIFFERENT CULTURE CONDITIONS. Fernandes, C.B.; Siqueira-Pyles, E.S.C.; Peres, K.R.; Ponchirolli, C.B.; Cordeiro Jr., F.; Alvarenga, M.A.; Landim-Alvarenga, F.C.

14:45 – 15:00: BOVINE EMBRYO PRODUCTION BY INTRACYTOPLASMIC LYOPHILIZEDSPERM INJECTION. PRELIMINARY RESULTS. Martins, C.F.; Dode, M.A.N.;Pereira, D.C.; Rumpf, R.

15:00 – 15:15: IDENTIFICATION OF THE DIFFERENTIAL EXPRESSION OF PI3K IN BOVINEOOCYTES AND EMBRYOS BY DIFFERENTIAL DISPLAY PCR (DDPCR).Ripamonte, P.; Emanuelli, I.P.; Mesquita, L.G.; Cortezzi, S.S.; Biase, F.H.; Merighe,G.K.F; Caetano, A.R.; Meirelles, F.V.

15:15 – 15:30: cDNA AMPLIFIED FROM BOVINE OOCYTES. Biase, F.H.; Mingoti, G.Z.,Ripamonte, P.; Cortezzi, S.S.; Merighe, G.K.F.; Martelli, L.; Meirelles. F.V.


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CONTENTSActa Scientiae Veterinariae 32(Suplement), 2004

President Letter ............................................................................................................................... IIIAcknolowdgments ............................................................................................................................. VProgram ............................................................................................................................................VI

ConferencesHORMONAL MANIPULATION OF THE ESTROUS CYCLE IN BOVINE EMBRYODONORS AND RECIPIENTS. G.A. Bó, D. Moreno, L. Cutaia, P.S. Baruselli, E.L. Reis. ............................ 1

RECENT ADVANCES IN HORMONAL TREATMENTS USED FOR FIXED-TIME ARTIFICI-AL INSEMINATION (FTAI) IN BEEF CATTLE. C.M. Barros AND R.L.Ereno. ............................................................................................................................................................... 23

FERTILIZATION AND EMBRYONIC MORTALITY IN CATTLE R. Sartori. ........................................ 35

QUALITY CONTROL GUIDELINES FOR IN VITRO EMBRYO PRODUCTIONLABORATORIES A.G. Galuppo. ................................................................................................................ 51

OOCYTE TRANSFER AND INTRACYTOPLASMIC SPERM INJECTION IN HORSES.M.A. Coutinho da Silva. .................................................................................................................................. 55

SORTED SEMEN APPLICATION IN PRODUCTIVE ANIMALS. M. Medina. ......................................... 65

ANIMAL CLONING AND PLACENTATION. M.A. Miglino. .................................................................. 75

Round Table Discussion

CRYOPRESERVATION OF IN VITRO PRODUCED EMBRYOS. A. Mezzalira ...................................... 81

CRYOPRESERVATION OF BOVINE OOCYTES. A. D. Vieira AND J. L. Rodrigues ............................ 83

CRYOPRESERVATION OF IN VITRO PRODUCED BOVINE EMBRYOS. Y.F. Watanabe .................. 84

Oral Presentation

INTERFERON-TAU SECRETION IN CRYOPRESERVED IN VITRO PRODUCED BOVINEEMBRYO. Araujo, M.C.C.; Ferreira, A.M.; Sá, W.F.; Barreto Filho, J.B.; Serapião,R.V.; Camargo, L.S.A.; Silva, M.V.G.B.; Vale Filho, V.R. ........................................................................... 87

INCREASED CONCEPTION RATE IN BOVINE EMBRYO RECIPIENTS WITH HIGHERPLASMATIC PROGESTERONE CONCENTRATION ON DAY OF EMBRYO TRANSFER. Reis,E.L.; Marques, M.O.; Carvalho, N.A.T.; Nasser, L.F.; Costa Neto, W.P.; Baruselli, P.S. ............................. 88

USE OF LONG ACTING PROGESTERONE FOR PREPARATION OF NON-CYCLIC EMBRYORECIPIENT MARES. Rocha Filho, A.N.; Pessôa, M.A.; Gioso, M.M.; Alvarenga, M.A. ....................... 89


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EVALUATION OF THE TRANSCERVICAL EMBRYO RECOVERY IN SANTA INES EWES.Silva, J.C.; Quintela, A.; Andrade Moura, J.C.; Resende, J.; Gordiano, H.; Martins,L.P.; Chalhoub, M.; Ribeiro Filho, A. de L.; Gusmão, A.L. ........................................................................... 90

CRYOPRESERVATRION AND SHORT-TERM CULTURE OF ISOLATED CAPRINEPRIMORDIAL FOLLICLES. Rodrigues, A.P.R.; Costa, S.H.F.; Santos, R.R.; Lucci, C.M.;Nunes, J.F.; Ohashi, O.M.; Rondina, D.; Figueiredo, J.R. .............................................................................. 91

DEVELOPMENTAL POTENTIAL OF BOVINE EMBRYOS FOLLOWING IN VITRO OOCYTEFERTILIZATION WITH CATALASE AND GnRH. Martins Júnior, A.; Calegari,R.S.; Freitas, C.P.; Zanon, J.E.O.; Paschoal. D.M. ......................................................................................... 92

EFFECT OF RETINOIDS AND IGF-I ON THE IN VITRO BOVINE EMBRYONICDEVELOPMENT. Oliveira, M.A.L.; Lima, P.F.; Reichenbach, H.-D.; Weppert, M.; CavalcantiNeto, C.C.; Pina, V.M.R.; Santos, M.H.B.; Lima-Verde, I.B. ........................................................................ 93

UTILIZATION OF A PORTABLE INCUBATOR (LIFETM) FOR IN VITRO PRODUCTION OFBOVINE EMBRYOS. Siqueira-Pyles, E.S.C.; Pedrazzi, C.A.F.; Landim-Alvarenga, F.C. ......................... 94

EMBRYO PRODUCTION IN NELORE COWS USING IVF ASSOCIATED WITH ET. NonatoJr., I.; Rufino, F.A.; Sanches, B.V.; Pontes, J.H.F.; Uvo, S.; Ereno Jr., J.C.; Seneda, M.M. ......................... 95

EVALUATION OF EMBRYO SPLITTING AS AN ALTERNATIVE TO IMPROVE GESTATIONINDICES IN EMBRYO TRANSFER HORSES. Silveira, L.L.; Souza, R.V.; Pimentel, C.M.;Rumpf, R. ........................................................................................................................................................ 96

Students Competition

PUBERTY INDUCTION AND SYNCHRONIZATION OF THE ESTRUS WITH PROGESTERONE,ESTRADIOL BENZOATE, GNRH AND ECG IN PREPUBERAL HEIFERS OF THE NELOREBREED Tetzner, T.A.D.; Diniz, E.G.; Jacomini, J.O.; Peres, R.F.G.; Amorim, L.L. .................................... 99

OPTIMIZING THE NUMBER OF RECIPIENT COWS SYNCHRONIZED WITHOVSYNCH PROTOCOL. Beltrame, R.T.; Barioni, L.G.; Veloso, R.V.; Saueressig, M.G. ....................... 100

CITOPLASMATIC MORFOLOGY EVALUATION OF EQUINE OOCYTES MATURED INDIFFERENT CULTURE CONDITIONS. Fernandes, C.B.; Siqueira-Pyles, E.S.C.; Peres,K.R.; Ponchirolli, C.B.; Cordeiro Jr., F.; Alvarenga, M.A.; Landim-Alvarenga, F.C. ................................. 101

BOVINE EMBRYO PRODUCTION BY INTRACYTOPLASMIC LYOPHILIZED SPERMINJECTION. PRELIMINARY RESULTS. Martins, C.F.; Dode, M.A.N.; Pereira, D.C.; Rumpf, R. ...... 102

IDENTIFICATION OF THE DIFFERENTIAL EXPRESSION OF PI3K IN BOVINE OOCYTESAND EMBRYOS BY DIFFERENTIAL DISPLAY PCR (DDPCR). Ripamonte, P.; Emanuelli, I.P.;Mesquita, L.G.; Cortezzi, S.S.; Biase, F.H.; Merighe, G.K.F; Caetano,A.R.; Meirelles, F.V. ..................................................................................................................................... 103

cDNA AMPLIFIED FROM BOVINE OOCYTES. Biase, F.H., Mingoti, G.Z., Ripamonte, P.;Cortezzi, S.S.; Merighe, G.K.F.; Martelli, L.; Meirelles. F.V. ..................................................................... 104


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Abstracts

IN VITRO DEVELOPMENT OF POOR QUALITY BOVINE EMBRYOS SUBMMITED TO TREEDIFFERENT CULTURE MEDIUM. Alvarez, R.H.; Pires, R.M.L.; Martinez, A.C.;Meirelles, F.V. ............................................................................................................................................... 107

ROSCOVITINE AND ANTIOXIDANTS ASSOCIATION IN BOVINE OOCYTES IN VITRONUCLEAR MATURATION Barretto, L.S.S.; Tavares, D.C.; Perecin, F.; Mingoti,G.Z. ................................................................................................................................................................ 108

EVALUATION OF CULTURE SYSTEM FOR IN VITRO PRODUCTION OF BOVINE EMBRYOS.Miranda, M.S.; Amarante, M.; Cordeiro M.S.; Biondi, F.C.; Sousa, J.S.; Ohashi,O.M. ............................................................................................................................................................... 109

THE EFFECTS OF THE IBMX (3 ISOBUTYL, 1 METHYL-XANTHINE) ON NUCLEAR MATU-RATION IN BOVINE OOCYTE IN VITRO. Tavares, D.C.; Barretto, L.S.S.; Mingoti,G.Z. ................................................................................................................................................................ 110

DNA FRAGMENTATION ANALYSIS OF IN VITRO PRODUCED BOVINE EMBRYOSFOLLOWING OOCYTE MATURATION IN THE PRESENCE OF DIFFERENTMACROMOLECULE SOURCES. Lo Turco, E.G.; Barretto, L.S.S.; Perecin, F; Soria, G.;Bertolla, R.P., Mingoti, G.Z. ......................................................................................................................... 111

ANGIOTENSIN II REVERSES THE INHIBITORY ACTION OF FOLLICULAR CELLS ON BO-VINE OOCYTE NUCLEAR MATURATION REGARDLESS IGF-I PRESENCE Stefanello, J.R.; Porciuncula, P.M.; Costa, L.F.S.; Oliveira, J.F.C.; Gonçalves, P.B.D. ............................. 112

EMBRYONIC DEVELOPMENT AND APOPTOSIS OF IN VITRO-DERIVED BOVINEEMBRYOS Mesquita, L.G.; Carambula, S.F.; Emanuelli, I.P.; Ripamonte, P.; Merighe, G.K.F.;Meirelles, F.V. ............................................................................................................................................... 113

NUCLEAR MATURATION AND EMBRYONIC DEVELOPMENT OF OOCYTES MATUREDIN MEDIUM WITH DIFFERENT MACROMOLECULES IN PRESENCE OR ABSENCE OFCUMULUS CELLS. Ancioto, K.L.; Vantini, R.; Garcia, J.M.; Mingoti, G.Z . ..................................... 114

INFLUENCE OF CUMULUS CELLS AND THE BREED OF BULL ON THE DEVELOPMENTOF BOVINE EMBRYOS SUBMITED TO HEAT STRESS Eberhardt, B.G.;Karpinzaiki, C.R.L.; Satrapa, R.A; Barros, C.M. .......................................................................................... 115

USE OF DIFFERENT MEDIUM AND HORMONAL TREATMENTS IN SWINE OOCYTE MATU-RATION Marques, M.G.; Gerger, R.P.C.; Oliveira, V.P.; Nascimento, A.B.; Visintin,J.A. ................................................................................................................................................................. 116

EFFECTS OF MACROMOLECULES ON CUMULLUS-OOCYTE COMPLEXES ANDSUBSEQUENT IN VITRO BOVINE EMBRYONIC DEVELOPMENT Emanuelli, I.P.; Perecin,F.; Marques, M.G.; Mesquita, L.G.; Cortezzi, S.S; Merighe, G.K.F; Meirelles, F.V. .......................... 117

TWO-DIMENSIONAL ELETROPHORESIS ANALYSIS OF IN VITRO MATURED BOVINEOOCYTES PROTEINS IN THE PRESENCE OR ABSENCE OF CUMULUSCELLS Ancioto, K.L.; Vantini, R.; Roncoleta, M.; Mingoti, G.Z. ............................................................... 118


XIII

CULTURE OF IN VITRO PRODUCED BOVINE EMBRYOS: EFFECT OF NUMBER OFZYGOTES AND VOLUME OF MEDIUM. Brum, D.S.; Leivas, F.G.; Mozzaquatro, F.D.;Fialho, S.S.; Bernardi, M.L.; Rubin, M.I.B.; Silva, C.A.M. ........................................................................ 119

EVALUATION OF A CONTINUOUS INFUSION PUMP FOR OVUM PICK-UP (OPU) INBOVINE. Rubin, K.C.P.; Rigo, A.G.; Schroeder, R.V.; Silva, R.C.P.; Marques, M.O.;Seneda, M.M. ................................................................................................................................................ 120

ADDITION OF RETINOIDS AND FSH TO THE MATURATION MEDIUM OF OOCYTES ANDTHEIR EFFECTS ON IN VITRO PRODUCTION OF BOVINE EMBRYOS Lima, P.F.; Oliveira,M.A.L.; Reichenbach, H.-D.; Weppert, M.; Cavalcanti Neto, C.C.; Pina, V.M.R.;Santos, M.H.B.; Loureiro, B. ......................................................................................................................... 121

USE OF INSULINE IN BOVINE EMBRYO CO-CULTURE. Lima-Verde, I.B.; Pina, V.M.R.; San-tos, M.H.B.; Loureiro, B.; Cavalcanti Neto, C.C.; Iunes-Souza, T.C.; Moura, R.T.N.;Bartolomeu, C.C.; Oliveira, M.A.L. .............................................................................................................. 122

USE OF COCONUT WATER IN MATURATION MEDIUM IN VITRO OF CANINE OOCYTESPina, V.M.R.; Oliveira, M.A.L; Lima, P.F.; Moura, R.T.N.; Alves, J.R.; Araújo,S.S.; Melo, A.N.; Aguiar Filho, C.R. ............................................................................................................ 123

USING THE POST HATCHING DEVELOPMENT (PHD) SYSTEM FOR MONITORING BOVINEIVP EMBRYOS DEVELOPMENT. Brandão, D.O.; Vajta, G.; Callesen, H.;Rumpf, R. ...................................................................................................................................................... 124

MORPHOLOGICAL EVALUATION OF PORCINE OOCYTES EXPERIMENTALLY EXPOSEDTO THE PORCINE PARVOVIROSE VIRUS DURING IN VITRO MATURATION TIME D’Angelo,M.; Athayde, C.S.; Souza, R.J.; Carvalho, J.F.; Zerio,N.M.C.; Melo, G.M.; Galuppo, A.G.; Bersano, J.G. ..................................................................................... 125

CLASTOGENIC ACTION OF BOVINE HERPESVIRUS TYPE 1 (BHV-1) IN IN VITROMATURATED BOVINE OOCYTES. D’Angelo, M.; Melo,G.M.; Athayde, C.S.; Souza, R.J.;Zerio, N.M.C.; Rojas, N.; Carvalho, J.F.; Suzuki, M.F. ................................................................................ 126

FREEZING AND STOCK OF READY TO USE MEDIUM: THE SIMPLIFIED IVP ROUTINE OFBOVINE EMBRYOS. Brandão, D.O.; Pereira, D.C.; Dode, M.A.N.;Rumpf, R. ...................................................................................................................................................... 127

BOVINE GRANULOSA CELL RECOVER BY ULTRASOUND-GUIDED FOLLICULARASPIRATION: PARTIAL RESULTS. Vireque, A.A.; Viana, J.H.M.; Rosa e Silva, A.A.M.;Sá, W.F.; Ferreira, A.M.; Camargo, L.S.A. ................................................................................................. 128

EFFECTS OF TWO DIFFERENT MEDIUMS IN OOCYTE IN VITRO MATURATION INPRÉ-PUBERTY LAMBS. Capezzuto, A.; Meirelles, F.V.; Merighe, G.K.F.; Traldi, A.S. ......................... 129

USE OF VAPORIZATED COCONUT WATER FOR IN VITRO SPERMATIC CAPACITATIONIN SWINE. Nascimento, A.B.; Visintin, J.A.; Oliveira, V.P.; Marques,M.G.; Gerger, R.P.C.; Toniolli, R.; Seneda, M.M. ....................................................................................... 130

EVALUATION OF SPERM CAPACITATION INDUCED BY HEPARIN IN DOGS Lucio, C.F.;Vannucchi, C.I.; Oliveira, V.P.; Gonçalves, J.S.A.; Visintin, J. A.; Nichi, M.; Assumpção,M.E.O.A. ....................................................................................................................................................... 131


XIV

PRETREATMENT OF BOVINE SPERM WITH DITHIOTHREITOL (DTT) AND HEPARIN TOTHE INTRACYTOPLAMIC SPERM INJECTION (ICSI). Cortez, C.,Fontes, R.S., Matos, L.F. ............................................................................................................................... 132

RATES OF PRONUCLEI FORMATION AND NON-PARTHENOGENETIC DEVELOPMENT OFBOVINE EMBRYOS PRODUCED BY ICSI Matos, L.F.; Fontes,R.S.; Quirino, C.R.; Buitkamp, J.; Weppert, M.; Reichenbach, H.-D. ......................................................... 133

EFFECT OF DIFERENT CONCENTRATIONS OF INDOL ACETIC ACID IN THE CULTUREOF OVINE PREANTRAL FOLLICLES. Andrade, E.R.; Seneda, M.M.; Alfieri,A.A.; Oliveira, J.A.; Boselli, C.C.; Rubin, K.C.P.; Figueiredo, J.R.; Toniolli, R. ........................................ 134

EFFECT OF 0.9% SALINE SOLUTION AND TCM 199 AT DIFFERENT TEMPERATURES ANDINCUBATION TIMES ON THE MORPHOLOGY OF BOVINE PREANTRAL FOLLICLES.Celestino, J.J.H.; Santos, R.R.; Matos, M.H.T.; Costa, S.H.F.; Silva, J.R.V.; Martins, F.S.; Silva,G.A.; Figueiredo, J.R. .................................................................................................................................... 135

DEVELOPMENT OF GOAT PREANTRAL FOLLICLES IN COCONUT WATER SOLUTION.Martins, F.S.; Santos, R.R.; Celestino, J.J.H.; Matos, M.H.T.; Silva, G.A.;Ferreira, F.V.A.; Figueiredo, J.R. .................................................................................................................. 136

CYTOGENETIC ANALYSIS OF BOVINE AND MURINE EMBRYOS. Marques, D.M.;Carelli, J.B.; Goldschmidt, B. ....................................................................................................................... 137

FSH INCREASES FIBROBLAST GROWTH FACTOR RECEPTOR-3c (FGFR-3c) GENEEXPRESSION IN CULTURED BOVINE GRANULOSA CELLS. Buratini Jr., J.; Cao, M.;Castilho, A.C.S.; Andrade, P.B.; Price, C.A. ................................................................................................ 138

FIBROBLAST GROWTH FACTOR 10 (FGF-10) GENE EXPRESSION IN BOVINE ANTRALFOLLICLES. Pinto, M.G.L.; Price, C.A.; Giometti, I.C.; Costa, I.B.; Teixeira,A.B.; Barros, C.M.; Buratini Jr., J. ................................................................................................................ 139

DETECTION OF IMPRINTED MESSENGER RNA (mRNA) EXPRESSION IN CUMULUS,FIBROBLASTS, PLACENTA AND PREIMPLANTION EMBRYOS OF BOVINE. Franco, M.M.;Melo, E.O.; Mundim, T.C.D.; Pereira, D.C.; Iguma, L.T.; Ávila, F.F.; Rumpf, R. ..................................... 140

CHARACTERIZATION OF FIBROBLASTOS FETAL AND ADULT OF NELORE BOVINES FORNUCLEAR TRANSFERENCE. Caetano, H.V.A.; Milazzotto, M.P.; Assumpção, M.E.O.A.;Visintin, J.A. .................................................................................................................................................. 141

NUCLEAR TRANSFER POTENTIAL OF ROSCOVITINE MEIOTIC INHIBITEDBOVINE OOCYTES. Merighe, G.K.F.; Watanabe, Y.F.; Leal, C.L.V.; Meirelles, F.V. .......................... 142

PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES USING CYTOCHALASIN B ORD IN ASSOCIATION WITH CYCLOHEXIMIDE. Forell, F.; Feltrin, C.; Vieira,A.D.; Antoniolli, C.B.; Rodrigues, J.L. ......................................................................................................... 143

PRONUCLEAR EMBRYO PRODUCTION FOR DNA EXOGEN MICROINJECTION INPELIBUEY SHEEP SUPEROVULATED WITH FHS-P AND GnRH. Solano, R.F.; DeArmas, R.T.; Castro, F.O.; Aguilar, A.P. ...................................................................................................... 144

PRODUCTION OF BOVINE CHIMAERIC EMBRYOS USING INNER CELL MASS CELLS ORBOVINE EMBRYONIC STEM LIKE CELLS (ES-LIKE-CELLS) Wolf, A.;Gabaldi, S.H.; Garcia, J.M. ........................................................................................................................... 145


XV

CHEMICAL ENUCLEATION OF BOVINE OOCYTES BY COLCHICINE. Saraiva, N.Z.;Perecin, F.; Costa, P.F.; Ferreira, C.R.; Méo, S.C; Garcia, J.M .................................................................... 146

IN VITRO TRANSFECTION OF BOVINE MYOBLASTS FOR CELL-MEDIATED GENETRANSFER. Milazzotto, M.P.; Caetano, H.V.A.; Nicácio, A.C.; Simões, R.; Yamada, C.;Visintin, J.A. .................................................................................................................................................. 147

FETAL EXPRESSION OF MYOSTATIN GENE IN NELORE (BOS PRIMIGENIUS INDICUS)SKELETAL MUSCLE. Milazzotto, M.P.; Martins, L.; Caetano, H.V.A.; Feitosa,W.B.; Assumpção, M.E.O.A.; Visintin, J.A. ................................................................................................. 148

SIMULTANEOUS RNA, DNA AND PROTEIN ISOLATION FROM BOVINE FETAL OVARIANAND TESTICULAR TISSUE. Siqueira, F.; Grisolia, A.B.; Santos, S.E.C.; Nunes,C.M.; Lopes, C.R.; Garcia, J.F. ..................................................................................................................... 149

ALTERNATIVE MHC MOLECULE EXPRESSION IN BOVINE AND MURINE PLACENTASFROM CLONED AND NON-CLONED ANIMALS. Puelker, R.Z.; KfouryJr, J.R.; Verechia, F.T.; Meirelles, F.V. ...................................................................................................... 150

PLACENTATION IN THE CLONED CATTLE. Pereira, F.T.V.; Braga, F.C.; Assis Neto, A.C.;Ambrósio, C.E.; Kfoury Jr., J.R.; Oliveira, L.J.; Papa, P.C.; Carvalho, A.F.; Santos, T.C.; Visintin,J.A.; Mello, M.B.; Garcia, J.M.; Yamazaki, W.; Rumpf, R.; Iguma, L.T.; Carter,A.M.; Leiser, R.; Miglino, M.A. ................................................................................................................... 151

PLACENTAL ERYTROPHAGOCITOSYS IN WATER BUFFALO. Pereira, F.T.V.; Braga, F.C.;Kfoury Jr., J.R.; Oliveira, L.J.; Papa, P.C.; Carvalho, A.F.; Kohayagawa, A.; Hamlett,W.C.; Miglino, M.A. ..................................................................................................................................... 152

MICROSCOPIC DEVELOPMENT OF BOVINE FETAL MEMBRANES Assis Neto, A.C.;Miglino, M.A.; Verechia, F.T., Garcia, J.M. ................................................................................................. 153

THE FEMALE REPRODUCTIVE TRACT AND PLACENTA IN SOUTH AMERICAN WILD PIGS(Tayassu tajacu AND Tayassu peccari). Santos, T.C.; Oliveira, M.F.; Miglino, M.A.; Dantzer V. ..................................................................................................................................................... 154

STUDY OF VEINED ANGIOARCHITECTURE OF THE GENITAL ORGANS OF BOVINE Gioso,M.M.; Costa, E.P.; Fernandes, C.A.C.; Paula, T.A.; Bittencourt, V.L.; Guimarães,J.D.; Siqueira, L.G.B. .................................................................................................................................... 155

CRYOPRESERVATION OF RABBITS EMBRYOS (Oryctologus cuniculus) WITH GLYCEROL,ETHILENE-GLYCOL AND GLYCEROL PLUS SUCROSE. Solano, R.F.; Solano, G.O.;Chow, L.A. .................................................................................................................................................... 156

EVALUATION OF CELULAR DEATH IN CRYOPRESEVATED MOUSE EMBRYOS Coutinho,A.R.S.; Mendes, C.M.; Dagli, M.L.Z.; Sinhorini, I.L.; Visintin, J.A.; Assumpção,M.E.O.A. ....................................................................................................................................................... 157

EFFECT OF NUMBER OF EMBRYOS PER FLUSH ON PREGNANCY RATE OF BOVINEEMBRYOS FROZEN IN ETHYLENE GLYCOL. Oliveira, E.R.; Fernandes,C.A.C.; Figueiredo, A.C.S.; Gioso, M.M. ..................................................................................................... 158

STAGE OF DEVELOPMENT AND PREGNANCY RATES OF FROZEN BOVINE EMBRYOS INETHYLENE GLYCOL. Fernandes, C.A.C.; Oliveira, E.R.; Figueiredo,A.C.S.; Gioso, M.M. ...................................................................................................................................... 159


XVI

EFFECT OF THE INTERACTION BETWEEN FREEZING AND OTHER SOURCES OFVARIATION ON PREGNANCY RATES OF F2 HOLSTEIN-GIR EMBRYOS. Freitas,C.; Palhão, M.P.; Viana, J.H.M.; Arashiro, E.K.N.; Nogueira, L.A.G.; Sá, W.F. ........................................ 160

PREGNANCY RATES OF FRESH AND FROZEN-THAWED EMBRYOS TRANSFERRED INHIGH PRODUCTION HOLSTEIN COWS. Rodrigues, C.A.; Ayres,H.; Reis, E.L.; Nichi, M.; Bo, G.A.; Baruselli, P.S. ...................................................................................... 161

USE OF ETILENE-GLYCOL, DIMETHILSULFOXID AND DIMETHILFORMAMIDE IN THEVITRIFICATION OF BOVINE EMBRYOS IN OPEN PULLED STRAWS Siqueira-Pyles, E.S.C.; Fernandes, C.B.; Landim-Alvarenga, F.C. ............................................................................. 162

CRYOPRESERVATION AND IN VITRO CULTURE OF SHEEP PREANTRAL FOLLICLES.Santos, R.R.; Rodrigues, A.P.R.; Costa, S.H.F.; Silva, J.R.V.; Martins, F.S.;Matos, M.H.T.; Celestino, J.J.H.; Silva, G.A.; Figueiredo, J.R. ................................................................... 163

TRANSFER OF IN VITRO PRODUCED BOVINE EMBRYOS : EFFECT OF TRANSPORT ANDVITRIFICATION. Mezzalira, A.; Santos, R.M.; Barreta, M.; Cucco, D.; Mezzalira,J.C.; Wentz, K.C; Cruz, F.B. ......................................................................................................................... 164

VIABILITY OF BOVINE OOCYTES IN VITRO MATURED EXPOSED IN TREHALOSESOLUTION. Martins, R.P.; Dias, A.J.B.; Quirino, C.R. ............................................................................. 165

IN VITRO PENETRATION OF VITRIFIED SWINE OOCYTES USING SWINE SEMEN STOREDFOR UP TO 72 H. Macedo Jr., M.M.; Deschamps, J.C.; Lucia Jr., T.; Bordignon,J.; Serret, C.G.; Rambo, G.; Rheingantz, M.G.T. ......................................................................................... 166

THE INFLUENCE OF COLLECTION METHODS ON THE SEMINAL PARAMETERS OF SANTAINES RAMS. Alves, S.G.G.; Chalhoub, M.; Bittencourt, R.F.; Almeida, A.K.; Portela, A.P.M.;Freitas, D.S.; Meneses, M.A.; Ribeiro Filho, A. de L. .................................................................................. 167

INTRACELLULAR CALCIUM CONCENTRATION IN SPERMATOZOA TREATED WITHCALCIUM ION CHANNEL BLOCKERS Gabaldi, S.H.; Esper, C.R.; Tedesco, A.C.;Wolf, A.; Oliveira, J.A. ................................................................................................................................. 168

ADJUSTING SPERM CONCENTRATION USED TO INSEMINATE SUPERSTIMULATEDNELORE COWS, IN ORDER TO AVOID DECLINE IN EMBRYO PRODUCTION.Nogueira, M.F.G. ; Barros, C.M. ................................................................................................................... 169

EFFECT OF SPERMATOZOA NUMBER, SITE OF SEMEN DEPOSITION AND SPERMATOZOASELECTION BY PERCOLL ON THE POST INSEMINATION UTERINE INFLAMMATORYRESPONSE WITH STALLION FROZEN SEMEN. Gomes, G. M.;Leão, K.M.; Macedo, L.P.; Jacob, J.C.F.; Papa, F.O.; Oliveira, J.V.; Alvarenga, M.A. ........................... 170

EFFECT OF SIRE ON PREGNANCY LOSS IN LACTATING HOLSTEIN COWS LIMA,F.S.; VASCONCELOS, J.L.M.; GARCIA, P.H. ........................................................................................... 171

BULL EFFECT ON IN VITRO PRODUCTION OF GIROLANDO BOVINE EMBRYOSBrum, D.S.; Leivas, F.G.; Palma, G.A.; Olivier, N. .................................................................................... 172

EVALUATION OF DIFFERENT ARTIFICIAL INSEMINATION TECHNIQUES USING LOWNUMBER OF SPERMATOZOA IN MARES. Leão, K.M.; Puoli-Filho, J.N.P.;Alvarenga, M.A. ............................................................................................................................................ 173


XVII

EFFECT OF DIFFERENTS BLOOD COMPOUNDS ON EQUINE SPERMATIC MOTILITY.Araújo, E.B.; Silva, J.F.S.; Souza, G.V.; Detmann, E.; Cunha, I.C.N.; Fagundes, B.;Carvalho, C.S.P. ............................................................................................................................................ 174

STRUCTURAL AND FUNCTIONAL EVALUATION OF EQUINE SPERMATOZOA EXPOSEDTO DIFFERENT CRIOPROTECTORS. Neves Neto, J.R.; Fernandes, C.B.; Landim-Alvarenga, F.C.;Papa, F.O. ...................................................................................................................................................... 175

EFFICIENCY OF A NEW EXTENDER ON BOVINE SEMEN FREEZEABILITY Alberti, K.; Carmo,M.T.; Oba, E.; Mendonça, A.L.Z.; Souza, D.B.; Dell’Aqua Jr., J.A.; Vianna, F.P.; Papa, F.O. .................. 176

REFREEZING OF PANTANEIRO STALLION SEMEN. Zúccari, C.E.S.N.; Nunes, D.B.;dePaula, F.A.L.; Ferreira, C.S.; Costa e Silva, E.V. ...................................................................................... 177

CRYOPRESERVATION OF SEMEN IN Leopardus tigrinus: COMPARISON BETWEEN TWODILUENTS. Tebet, J.M.; Martins, M.I.M.; Chirinea, V.H.; Souza, F.F.; Adania, C.H.;Lopes, M.D. ................................................................................................................................................... 178

RECTAL, ESCROTAL AND INTRATESTICULAR TEMPERATURE AND NUMBER OFSUDORIPAROUS GLANDS IN SCROTUM OF BUFFALOES (Bubalus bubalis). Satrapa, R.A.;Satrapa, R.; Diniz, E.G.; Beleti, M.E.; Eberhardt, B.G. ................................................................................ 179

SCROTAL CIRCUNFERENCE OF SANTA INES RAMS AND ITS RELATIONSHIP WITH BODYWEIGHT, AGE AND CORPORAL MEASURES. Bittencourt, R.F.; Chalhoub, M.; Alves,S.G.G.; Almeida, A.K.; Biscarde, C.E.A.; Freitas, D.S.; Ferreira, A.B.C.;Ribeiro Filho, A. de L. ................................................................................................................................... 180

TESTICULAR LEYDIG CELL IDENTIFICATION OF CAPYBARA (Hydrochoerus hydrochaeris)BY CITOCROMO P450 AROMATASE ACTION SITE LOCALIZATION. Batalha,L.M.; Oba, E.; Laufer Amorim, R. ................................................................................................................ 181

MOLAR MASS OF PROSTATIC AND COAGULATING GLANDS PROTEINS OFCAPYBARA (Hydrochoerus hydrochaeris). Batalha, L.M.; Oba, E.; Souza, F.F. de ................................. 182

PROTOCOL FOR OPTIMIZATION OF THE POLIMERASE CHAIN REACTION ( PCR ) FORSEX DETERMINATION OF BOVINE EMBRYOS IN DIFFERENTS STAGES OFDEVELOPMENT. Polisseni, J.; Camargo, L.S.A.; Sá, W.F.; Machado, M.A.; Ferreira,A.M.; Oliveira, R.S; Ramos, A. .................................................................................................................... 183

EVALUATION OF IN VITRO VIABILITY OF MICROMANIPULATED IN VIVO PRODUCEDBOVINE EMBRYOS FOR SEX IDENTIFICATION AND EMBRYOTRANSFER OPTIMIZATION. Melo, L.F.; Souza, R.V.; Dode, M.A.N.; Rumpf, R. ................................ 184

FETAL SEXING IN OVINE. Andrade, J.C.O.; Guido, S.I.; Sousa, B.P.A. ............................................... 185

PLUSET PRIOR FOLLICLE ASPIRATION: IMPACT ON IN VITRO EMBRYO PRODUCTIONIN Bos indicus COWS. Blaschi, W.; Andrade, E.R.; Nonato Jr., I.; Pontes, J.H.F.; Ereno Jr.,J.C.; Uvo, S.; Seneda, M.M. .......................................................................................................................... 186

PRE-SYNCHRONIZATION OF COWS FOR CUMULUS-OOCYTE COMPLEXES RECOVER:PARTIAL RESULTS. Viana, J.H.M.; Palhão, M.P.; Arashiro, E.K.N.; Ferreira, A.M.;Fonseca, J.F.; Fernandes, C.A.C. .................................................................................................................. 187


XVIII

SUPPLEMENTATION OF DIETS WITH SOYBEAN OIL AND TREATMENT WITH rBST FORIN VITRO EMBRYO PRODUCTION IN CATTLE. Pivato, I.; Sartori, R.; Cavalieri,F.B.; Pereira, D.C.; Rumpf, R. ...................................................................................................................... 188

PLASMATIC PROGESTERONE AND PREGNANCY RATES IN BOVINE EMBRYORECIPIENTS. Fernandes, C.A.C.; Oba, E.; Figueiredo, A.C.S. ................................................................. 189

OVARIAN STRUCTURES IN EMBRYO DONNORS AND ITS RELATIONSHIP WITH PGF-ESTRUS INTERVAL. Fernandes, C.A.C.; Viana,J.H.M.; Oba, E.; Figueiredo, A.C.S.Oliveira, E.R. ................................................................................................................................................. 190

PROTOCOL P36 IS NOT DETRIMENTAL TO OOCYTES FROM NELORE (Bos indicus)EMBRYO DONORS. Melo, D.S.; Ferreira M.M.G.; Monteiro, F.M.; Potiens, J.R.; Barros,C.M. ............................................................................................................................................................... 191

EFFECT OF UTERINE REWASH IN ENBRYO RECOVERY RATE OF BOER GOATSMachado, E.A.; Silva, A.P.; Farias Júnior, N.A. ........................................................................................... 192

ASSOCIATION BETWEEN OPU RESULTS AND EMBRYO RECOVER AFTERSUPEROVULATION IN GIR BREED COWS. Viana, J.H.M.; Arashiro, E.K.N.; Freitas,C.; Palhão, M.P.; Fonseca, J.F. ...................................................................................................................... 193

PREGNANCY RATE OF COWS AFTER TRANSFERENCE OF BISECTED EMBRYOSAND DEMI EMBRYOS CULTIVATED IN VITRO Amaral, J.B.; Oba, E.; Pires, R.M.L. ..................... 194

EFFICIENCY OF DIFFERENTS PROTOCOLS FOR ESTRUS SYNCHRONIZATION IN BOVINEEMBRYO RECIPIENTS Barreiros, T.R.R.; Borsato, E.A.; Ludwig Jr., H.E.; Marques, M.O.;Ribeiro Jr., M.; Silva, R.C.P.; Seneda, M.M. ................................................................................................ 195

EMBRYO PRODUCTION AND PREGNANCY RATE IN SUPERSTIMULATED NELOREDONORS TREATED WITH hCG AND LH TO INDUCE OVULATION Nogueira, M.F.G.;Buratini Jr., J.; Barros, C.M. ......................................................................................................................... 196

EVALUATION OF OVARIAN RESPONSE IN HEIFERS AND COWS EMBRYO DONORS BYRECTAL PALPATION AND ULTRASONOGRAPHY Leal, L.S.; Oba, E.; Fernandes, C.A.C.;Sá Filho, O.G. ............................................................................................................................................... 197

EMBRYONIC LOSS IN SUPEROVULATED EMBRYO RECIPIENTS (BOS INDICUS xBOS TAURUS) TREATED WITH eCG Reis, E.L.; Nasser, L.F.; Nichi, M.; Baruselli, P.S. .................... 198

RECIPIENT COWS SYNCHRONIZED TO FIXE TIME EMBRYO TRANSFER Beltrame,R.T.; Cavalca, L.G.; Verneck, W.C.; Santos, L.M.; Quirino, C.R.; Fontes, R.S. ......................................... 199

EMBRIONIC DEATH IN NONSURGICAL EQUINE EMBRYO TRANSFER Castro,R.P.R.; Pinho, T.G.; Queiroz, F.J.R.; Oliveira, G.D.M.; Costa, A.C. ........................................................... 200

INFLUENCE OF TIME OF THE YEAR ON THE EFFICIENCY OF BOVINE EMBRYO DONORSAND RECIPIENTS IN THE MIDDLE-NORTH REGION OF BRAZIL. Souza, J.A.T.; Carter, J.A.;Bacelar, F.H.; Portela, F.J.A.; Corrêa, C.A.; Santos, P.A.C. ........................................................................ 201

INFLUENCE OF RECIPIENT BREED, DONOR/RECIPIENT ESTRUS SYNCHRONY, TYPE OFCL AND P4 CONCENTRATIONS ON BOVINE RECIPIENT PREGNANCY RATES Souza, J.A.T.;Carter, J.A.; Bacelar, F.H.; Portela, F.J.A.; Corrêa, C.A.; Santos, P.A.C. .................................................... 202


XIX

INDUCTION OF PARTURITION ON SANTA INES SHEEP USED AS RECIPIENTS IN ANEMBRYOS’ TRANSFER PROGRAM. Chalhoub, M.; Bittencourt, R.F.; Portela, A.P.M.; Alves,S.G.G.; Almeida, A.K.; Freitas, D.S.; Ribeiro Filho, A. de L. ..................................................................... 203

TIMING OF ARTIFICIAL INSEMINATION IN SUPEROVULATED COWS. Sales, J.N.S.;Souza, J.C. ..................................................................................................................................................... 204

EMBRYO PRODUCTION IN MORADA NOVA (VARIETY WHITE) EWES SUBMITTED TOSUPEROVULATORY TREATMENT WITH pFSH. Maia, E.L.M.M.; Souza, A.L.; Arruda, I.J.;Lima, I.M.T.; Almeida, K.C.; Lopes-Júnior, E.S.; Paula, N.R.O.; Teixeira, D.I.A.; Rondina, D.;Villaroel, A.B.S.; Freitas, V.J.F. ................................................................................................................... 205

SUB-LUTEAL LEVELS OF PROGESTERONE, AFTER SUPERESTIMULATORY TREATMENT,MAY DECREASE EMBRYOS RECOVERY RATE. Melo, D.S.; Ferreira, M.M.G.; Nogueira,M.F.G.; Barros, C.M. .................................................................................................................................... 206

EFFECT OF THE ESTROUS SYNCHRONIZATION PROTOCOL ON THE SUPEROVULATORYRESPONSE IN A PROGRAM OF ZEBUS EMBRYO TRANSFER Ribeiro Filho, A. de L.;Bittencourt, R.F.; Portela, A.P.M., Freitas, D.S.; Almeida, A.K.; Silva, A.A.B.;Santana, R.C.M.; Chalhoub, M. .................................................................................................................... 207

SUPEROVULATORY TREATMENT ASSOCIATED WITH FREQUENT GnRHADMINISTRATION Melo, D.S.; Monteiro, F.M.; Nogueira, M.F.G.; Eberhardt, B.G.;Carvalho, L.M.; Sartorelli, E.S.; Trinca, L.A.; Barros, C.M. ........................................................................ 208

EVALUATION OF FOLLICULAR DYNAMIC IN MARES TREATED WITH EQUINEPITUITARY EXTRACT AND PURIFIED EQUINE FSH. Machado M.S.; Carmo, M.T.;Squires, E.L.; Roser, J.F. ; Alvarenga, M.A. ................................................................................................. 209

COMPARISON BETWEEN CONSTANT AND DECREASING DOSES OF EQUINE PITUITARYEXTRACT FOR THE INDUCTION OF SUPEROVULATION IN HAMSTERCarmo, M.T.; Siqueira-Pyles, E.S.C.; Landim-Alvarenga, F.C.; Alvarenga, M.A. ...................................... 210

“IN VITRO” VIABILITY AND ULTRASTRUCTURAL ANALYSIS OF EQUINE EMBRYOSCOMING FROM SUPEROVULATORY TREATMENT. Peres, K.R.; Fernandes, C.B.;Siqueira-Pyles, E.S.C.; Landim-Alvarenga, F.C.; Alvarenga, M.A.; Squires, E.L. ...................................... 211

HASTEN OF THE BREEDING SEASON AND INDUCTION OF MULTIPLE OVULATION INTRANSITIONAL MARES WITH PURIFIED EQUINE FOLLICLE STIMULATING HORMONE(eFSH). Peres, K.R.; Fernandes, C.B.; Siqueira-Pyles, E.S.C.; Landim-Alvarenga, F.C.;Alvarenga, M.A.; Squires, E.L. ..................................................................................................................... 212

EFFECT OF SUPEROVULATORY TREATMENT ASSOCIATED TO INTRAVAGINALPROGESTERONE DEVICE AND ESTRADIOL BENZOATE ON CONCEPTION RATE OFBOVINE RECIPIENTS. Sá Filho, O.G.; Vasconcelos, J.L.M.; Santos, R.M.; Lara, C.S.;Matos, S.P.M.; Jacob, M. .............................................................................................................................. 213

EFFECT OF INTRAVAGINAL PROGESTERONE DEVICE ASSOCIATED TO ESTRADIOLBENZOATE IN SUPEROVULATION PROGRAMS ON BOVINE SPECIES. Vasconcelos, J.L.M.;Sá Filho, O.G.; Santos, R.M.; Belisario, H.L.R.; Alvin, M.T.T.; Jaliba, W.P. ............................................. 214

SUCCESSIVE MULTIPLE OVULATIONS ON HOLSTEIN AND GIR NULIPAROUSDONORS. Lopes, B.C.; Ferreira, M.B.D.; Souza, J.C.; Azevedo, N.A.; Freitas, C.; Sales,J.N.S. ............................................................................................................................................................. 215


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HORMONAL CONTROL OF ESTRUS CYCLE OF ACYCLIC COWS. Dias, A.J.B.; Matos,L.F.; Fontes, R.S. ........................................................................................................................................... 216

SERUM PROGESTERONE CONCENTRATIONS IN CROSSBRED HEIFERS NELLORE/ANGUSTREATED WITH DIFFERENT INTRAVAGINAL PROGESTERONE DEVICE. Santos, R.M.;Vasconcelos, J.L.M.; Perez, G.C.; Sá Filho, O.G.; Maciel, A.B.B. .............................................................. 217

PREGNANCY RATE, AFTER FIXED-TIME ARTIFICIAL INSEMINATION (FTAI), IN NELOREDONORS SUBMITTED TO SEVERAL EMBRYO FLUSHING. Nogueira, M.F.G.;Barros, C.M. .................................................................................................................................................. 218

ARTIFICIAL INSEMINATION AND EMBRYO TRANSFER PREGNANCY RATES IN HIGHPRODUCTION HOLSTEIN (REPEAT BREEDER) BREEDINGS. Rodrigues, C.A.; Ayres, H.;Reis, E.L.; Nichi, M.; Bo, G.A.; Baruselli, P.S. ............................................................................................ 219

INCREASED PREGNANCY RATE IN NELORE COWS TREATED WITH eCG IN DIFFERENTPOST PARTUM PERIODS FOR FIXED TIME ARTIFICIAL INSEMINATIONRodrigues, C.A.; Ayres, H.; Reis, E.L.; Madureira, E.H.; Baruselli, P.S. .................................................... 220

EFFECT OF eCG AND GnRH ADMINISTRATION ON PREGNANCY RATE OF LACTATINGNELORE COWS SUBMITTED TO FIXED TIME ARTIFICIAL INSEMINATION. Silva, R.C.P.;Rodrigues, C.A.; Marques, M.O.; Ayres, H.; Reis, E.L.; Nichi, M.; Madureira, E.H.; Baruselli, P.S. ........ 221

EFFECT OF ESTRADIOL CYPIONATE OR BENZOATE TO INDUCE OVULATION ONPREGNANCY RATES IN FIXED TIME ARTIFICIALLY INSEMINATED NELORECOWS. Marques, M.O.; Ayres, H.; Reis, E.L.; Mapletoft, R.J.; Baruselli, P.S. ......................................... 222

EFFECT OF eCG AND WEAN ON PREGNANCY RATE OF LACTATING NELORE COWSFIXED TIME ARTIFICIALLY INSEMINATED. Penteado, L.; Ayres, H.; Reis, E.L.;Madureira, E.H.; Baruselli, P.S. .................................................................................................................... 223

EFECT OF TREATENT WITH TRIU-B FOR 7, 8 OR 9 DAYS ON PREGNANCY RATES INCROSS BRED ZEBU COWS AND HEIFERS INSEMINATED AT A FIXED-TIMEBalla, E.; Cledou, G.; Nosetti, L.; Maraña Peña, D.; Bó, G.A. ..................................................................... 224

EFECT OF TREATEMENT WITH NEW OR USED CIDR-B DEVICES ON PREGNANCY RATESIN CROSS BRED ZEBU COWS INSEMINATED AT A FIXED-TIME Bó, G.A.; Balla, E.;Venturini, M.; Tríbulo, R. ............................................................................................................................. 225

EFFECT OF eCG AND GnRH TREATMENTS IN BOS INDICUS COWS SUBMITTED TO FIXED-TIME ARTIFICIAL INSEMINATION. Duarte, R.A.; Reis, E.L.; Sá Filho, M.F.;Bó, G.A.; Baruselli, P.S.; Kozicki, L.E. ........................................................................................................ 226

SYNCHRONIZATION OF HEIFERS OVULATION WITH PROGESTERONE ANDPROGESTAGEN FOR FIXED TIME EMBRYO TRANSFER Rodrigues, C.A.; Mancilha,R.F.; Reis, E.L.; Mello, J.E.; Ayres, H.; Madureira, E.H.; Baruselli, P.S. .................................................... 227

EFFECT OF TREATMENT WITH eCG ON CONCEPTION RATE IN NELORE COWS WITHDIFFERENT BODY CONDITION SCORES SUBMITTED TO FIXED-TIME ARTIFICIALINSEMINATION (RETROSPECTIVE ANALYSIS) Baruselli, P.S.; Madureira, E.H.; Marques,M.O.; Rodrigues, C.A.; Nasser, L.F.; Silva, R.C.P.; Reis, E.L.; Sá Filho, M.F. .......................................... 228


XXI

EFFECT OF DIFFERENT DOSES OF hCG (VETECOR®) FOR SYNCHRONIZATION OF COWOVULATION ON FIXED TIME ARTIFICIAL INSEMINATION PROTOCOLMarques, M.O.; Campos Filho, E.P.; Mantovani, A.P.; Reis, E.L.; Nichi, M.; Baruselli, P.S. .................... 229

USE OF ARTIFICIAL INSEMINATION AS A ROUTINE IN REPRODUCTION PROGRAMS OFBULLDOG BREED BITCHES. Jacomini, J.O.; Moreira, C.F.; Cunha, G.N.; Diniz, E.G. ....................... 230

IMMUNOHISTOCHEMICAL DETECTION OF PROGESTERONE AND ESTROGENRECEPTORS IN NELORE (Bos taurus indicus ) COWS ENDOMETRIUM. Martin, I.;Ferreira, J.C.P.; Laufer Amorim, R.; Torres Neto, R.; Vettorato, L.F. ......................................................... 231

EFFECT OF PLASMATIC PROGESTERONE CONCENTRATION ON FOLLICULAR DYNAMICIN BOS INDICUS X BOS TAURUS HEIFERS Mantovani, A.P.; Sá Filho, M.F.; Reis, E.L.;Nichi, M.; Bó, G.A.; Baruselli, P.S. .............................................................................................................. 232

ULTRASONOGRAPHIC MONITORING OF UTERINE DYNAMIC IN NELORE (Bos taurusindicus) COWS DURING THE ESTROUS CYCLE. Martin, I.; Ferreira, J.C.P.;Vettorato, L.F.; Gioso, M.M.; Tavares, R.Z.; Mota, A.V.; Greatti, T.A.P.; Oba, E. .................................... 233

ULTRASONOGRAPHY CHARACTERISTICS OF CORPUS LUTEUM AND OVULATORYFOLLICLE DIAMETER IN NELORE COWS. Martin, I.; Vettorato, L.F.;Tavares, R.Z.; Greatti, T.A.P.; Gioso, M.M.; Mota, A.V.; Oba, E.; Ferreira, J.C.P. .................................... 234

FOLLICULAR DYNAMICS OF LACTATING ANESTROUS BEEF COWS TREATED WITHPROGESTAGEN, eCG AND GnRH. Sá Filho, M.F.; Reis, E.L; Viel Jr., J.O.; Nichi,M.; Madureira, E.H.; Baruselli, P.S. ............................................................................................................. 235

EFFECT OF ESTRADIOL CYPIONATE OR BENZOATE ON FOLICLE AND LUTEALDYNAMICS IN NELORE COWS. Reis, E.L.; Gimenes, L.U.; Marques, M.O.; Carvalho,J.B.P.; Mapletoft, R.J.; Baruselli, P.S. .......................................................................................................... 236

GOAT OVARIAN FOLLICULAR DYNAMIC EVALUATED WITH ULTRA-SOUND BYTRANSRECTAL AND TRANSVAGINAL MEANS Tenório Filho, F.; Santos, M.H.B.;Moraes, E.P.B.X.; Rocha, J.M.; Oliveira, L.R.S.; Oliveira, M.A.L.; Lima, P.F. .......................................... 237

SYNCHRONIZATION OF ESTRUS IN DAIRY TOGGENBURG GOATS DURING THEBREEDING SEASON. Fonseca, J.F.; Bruschi, J.H.; Santos, A.F.A. Maffili, V.V.; Moraes,E.A.; Pontes, R.A.; Prosperi, C.P. ................................................................................................................. 238

LOW DL-CLOPROSTENOL DOSE ADMINISTERED BY BAI-HUI ACUPOINT IN RIVERBUFFALOES COWS. Teixeira, A.B.; Dell’Aqua Jr., J.A.; Joaquim, J.G.F.; Siqueira-Pyles, E.S.C.;Zahn, F.S.; Carvalho, F.A.; Azevedo, H.C.; Leão, K.M.; Nogueira, M.F.G.; Oba, E. .......................................................................................................................................................... 239

EFFECTS OF ORAL TAURINE SUPPLEMENTATION IN REPRODUCTIVEPERFORMANCE OF DOMESTIC CATS. Moreira, C.F.; Jacomini, J.O.; Antônio, F.I. .......................... 240

EVALUATION OF EARLY PREGNANCY DIAGNOSIS IN CROSS BED FEMALE GOATS BYMEANS OF TRANS-RECTAL ULTRASOUND Cavalcante, P.V.T.H.; Solano,R.F.; Zaynette, F.T.; Moaraes Jr., F.J.; Solano, O.G. .................................................................................... 241

PREGNANCY RATE IN NELORE COWS, AFTER TEMPORARY CALF REMOVAL (TCR), ANDUSE OF HORMONAL PROTOCOLS WITH eCG. Souza, A.F.; Pinheiro,V.G.; Ereno, R.L.; Barros, C.M. .................................................................................................................... 242


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ULTRASSONOGRAFIC PREGNANCY DIAGNOSIS IN SHEEP USING TRANSRETAL ANDTRANSVAGINAL PROBES. Santos, M.H.B.; Moraes, E.P.B.X.; Guido, S.I.; Lima-Verde, I.B.; Rocha, J.M.; Bezerra, F.Q.G.; Iunes-Souza, T.C.; Oliveira, M.A.L.; Lima, P.F. ..................... 243

ASSOCIATION OF EARLY PREGNANCY DIAGNOSIS WITH ESTRUS RESYNCRONIZATIONIN ZEBU COWS. Freitas, D.S.; Portela, A.P.M.; Almeida, A.K.; Bittencourt, R.F.; Alves, S.G.G.;Gusmão, A.L.; Chalhoub, M.; Ribeiro Filho, A. de L. ................................................................................. 244

EVALUATION OF FEASIBILITY OF TWINNING IN CATTLE FOR BEEF PRODUCTIONIN PLANALTO CENTRAL. Lucas, L.A.; Pimentel, C.M.; Pivato, I.; Rumpf, R. ..................................... 245

Author Index ................................................................................................................................................ 246


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Conferences


XXIV


Documents

INDEXADO NO / INDEXED IN CAB International/ Index ... Ingles SBTE-2004 - Programa .pdf · José Buratini Jr. (2 o Secretário) Cezinande de Meira (1 o Tesoureiro) Flavio Meireles