Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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Welcome

BINDER Incubators and the introduction of the new BINDER KT 115 with Peltier technology

NEW

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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Contents to fulfill the objective

Introduction Technical Basics Portfolio Positioning KT – KB Competition USPs Product Launch

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.2 What is an Incubatorfor biological/medical applications?

WIKIPEDIA: “A device used to grow and maintain microbiological cultures or cell cultures”

The incubator maintains optimal growth conditions

• Temperature• Humidity• Other parameters, such as CO2 and O2

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.3 TemperatureDifferent ideal temperatures for different organisms

Mammalian cells 37 °C

Bacteria Escherichia coli (intestines) 37 °C Pseudomonas (ubiquitous) 28 °C Agrobacterium (soil) 21 °C Vibrio fischeri (sea) 15 °C Thermus aquaticus 74 °C

Yeast Saccharomyces cerevisiae 30 °C

Lab temperature 20-30 °C

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.3 TemperatureDifferent ideal temperatures for different organisms

laboratorytemperature

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.4 Incubators of different kindCool or uncool

Standard incubators Can only heat up Typical temperature range

• Minimum: 5 K to 10 K above ambient room temperature• Maximum: 60 °C to 100 °C

Typical application: bacterial incubation at 37 °C

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.5 Incubators of different kindCool or uncool

Cooling incubators Can heat up and cool down Typical temperature range

• Minimum: -5 °C to 5 °C• Maximum: 60 °C to 100 °C

Various applications• Incubation with heat input, e.g. by internal shaker• Incubation at high ambient temperatures• Incubation at 30 °C or lower

Cooling incubators with light Typical applications

• Plant growth• Insect growth• Photostability testing

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.6 Incubators of different kindCO2 or O2

CO2 Incubators Can only heat up Provide CO2 and humidity Provide O2 option Typical application: mammalian cell culture at 37 °C

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.7 Who needs Incubators?How and where to find customers

Universities and Research Institutes

Microbiology Genetics Zoology Biochemistry

Health Clinics, Hospitals Diagnostics Biomedicine Medical research Reproductive medicine Regenerative medicine

Industry Pharma Biotech Food & Beverage Cosmetics Contract research

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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1.8 Who needs Incubators?How and where to find customers

Areas Basic research Medical research Cell Biology Pharmacology Drug discovery Cell therapy In vitro fertilization (IVF) Genetic engineering

Applications Molecular biology Sterility testing Cell production Antibody production High throughput screening (HTS) Tissue engineering Model plant growth Germ identification

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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Contents to fulfill the objective

Introduction Technical Basics Incubator Portfolio Positioning KT – KB Competition USPs Product Launch

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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3.1 BINDER IncubatorsLarge portfolio

Standard incubators BD, BF

CO2 incubators C, CB

Cooling incubators KB

Cooling incubators with Peltier technology KT

Cooling incubators with light KBW, KBWF

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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3.2 BINDER IncubatorsLarge size selection: 28 different chambers

* KT 53 available end 2012

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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3.6 Common Features of BINDER IncubatorsAPT.line – Advanced Preheating Technology

Forced convection Fan-driven Chamber is surrounded by an air jacket Heat is distributed by a powerful fan Air is pulled into the preheating chamber Air is blown out horizontally

through small gaps in the side walls from both sides

Rapid heat distribution BF, KB, KT incubators

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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3.7 Common Features of BINDER IncubatorsEffective disinfection/sterilization

BINDER Incubators can heat up to at least 100 °C Providing a convenient and effective disinfection Important anti-contamination feature for microbiology

BINDER CO2 Incubators go even higher 180 °C automatic sterilization cycle Unique anti-contamination feature for serious cell culturing

180°C

100°C

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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Contents to fulfill the objective

Introduction Technical Basics Portfolio Positioning KT – KB Competition USPs Product Launch

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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2.1 Cooling TechnologyHow does vapor-compression refrigeration work?

Condenser

warm

sidehigh pressure

Waste heatChamber

Evap

orat

or

cold

sid

elo

w p

ress

ure

Cold air

Throttle Valve

refrigerantliquid

refrigerantvapor

Compressor

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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Peltier element Works as a heat pump,

releasing heat on one side and absorbing heat on the other side.

Effective heat sinks are mandatory.

current flow

waste heat

cold air

– +

chamber

2.2 Cooling Technology How does thermoelectric cooling work?

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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2.7 Vapor-compression CoolingWell-known application areas

Fridge (Refrigerator) Freezer Air conditioning

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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2.8 Thermoelectric CoolingBroad application areas1

Portable cooler (e.g. camping use) Air dehumidifier CPU cooler for high-performance

computers Small laboratory instruments Diode laser Thermocycler for PCR (molecular

biology labs) Sensor cooler in digital cameras

(e.g. for astro photography) GeneAmp® PCR System 9700 from Applied Biosystems® 2

1 Wikipedia 2 products,invitrogen.com

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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2.9 Peltier vs. Compressor TechnologyDistinctively different with convincing features

Advantages of the Compressor Greater heat compensation Extended temperature range to sub-zero Higher cooling-down rates High cooling efficiency

Advantages of the Peltier-Technology Safe (no flammable refrigerants) Eco-friendly (compliant to Roche K6 directive) Smaller and lighter (see right) Silent and gentle (less noise and vibrations) More robust and service-friendly Less expensive

• KB 115 Compressor (9.000 g)

• KT 115 Peltier-Chip (25 g)

• BINDER Pen (14 g)

Incubators including Peltier KT 115 September 2012STRICTLY CONFIDENTIAL - FOR COMPANY USE ONLY

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The BINDER Commitment

Best products Best services Best consulting