Upload
others
View
0
Download
0
Embed Size (px)
Citation preview
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-20
12
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
IN VIVO MOLECULAR IMAGINGAT CELLULAR RESOLUTION
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
Cel
lviz
io®
Dua
l Ban
d T
echn
ical
She
et ©
Mau
na K
ea T
echn
olo
gie
s, M
arch
20
13, 2
.00
Probe-based Confocal Laser Endomicroscope (pCLE) designed for in vivo Molecular Imaging of cellular to subcellular processes at two wavelengths.
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-20
12
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
Features
•Dual Band Excitation and Detection• In vivo and in situ Imaging
•Resolution up to 1,4 µm•Real-time smooth video recording at 9-50 fps
•A wide range of microprobes tailored to your application
• Monitor physiological interactions in vivo with minimal invasiveness• A large set of new applications including:Cancer and angiogenesis, neurobiology, inflammation,
immunology, biodistribution, colocalization studies...
•Easy to use and to install•Cutting-edge image processing
•Quantification features, Kinetics analysis•New, intuitive graphical interface
•Operating system: Linux-based, flexible
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
INSTRUCTIONS FOR USE en Cellvizio Dual Band Instructions for Use, Part C – February 2012
CELLVIZIO DUAL SOFTWARE
Screen Display
Menu bar
Tool buttons
C - 10/22
1
2
6 7 9
10
12
4
8
11
14
5
13
3
2
1
Technical Specifications
Excitation Wavelengths Laser 1: 488 nm Laser 2: 660 nm
Collection Bandwidths 502 - 633 nm 673 - 800 nm
Temporal Resolution 9 - 50 frames per second9 - 50 frames per second9 - 50 frames per second
Image Export .png, .bmp, . jpeg, .tiff, .mhd.png, .bmp, . jpeg, .tiff, .mhd
Movie Export .avi, .mpeg, .mp4, .swf, .avi, .mpeg, .mp4, .swf, .avi, .mpeg, .mp4, .swf,
Laser Class 2M2M
Dimensions and Weight 310 x 480 x 525 mm, 35 kg310 x 480 x 525 mm, 35 kg310 x 480 x 525 mm, 35 kg
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-20
12
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
Laser Scanning UnitConfocal microscope
488 and 660 nm simultaneous illumination
Real-time integration
Confocal ProcessorReal Time processing
Cellvizio Dual SoftwareAcquisition, QuantificationImage Processing
Confocal MicroprobeFiber-optics bundleFlexibleMiniaturized High-end objectives
Confocal Microprobes
* Beveled or Flat tip
Model ApplicationsTip
Diameter (mm)
Lateral Resolution
(µm)
Optical Sectioning
(µm)
Working Distance
(µm)
Max Field Of view
(µm)
Deep brain imaging, designed for permanent implantation on freely moving mice
Part of the NeuroPak™ solution
0,350,47 3,3 15 0 325
Brain, deep brain in mice, other organs at depth if low invasiveness is mandatory 0,3 3,3 15 0 300
Surface Imaging
Brain, deep brain in rats, other organs at depth if low invasiveness is mandatory 0,65 3,3 15 0 600
General applicability, can be used to check fluorescence sensitivity in most targets 1,5 3,3 15 0 600
Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness
2,6 1,4 10 60 240
Hi-ResImaging
Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness
4,2 1,4 10 30 240
ImagingVessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness
4,2 1,4 10 100 240
Blood flow through the vessel (without penetration) image deeper cell layers of tumor, organ or tissue
1,8 3,5 70100/170
at488/660
600
Depth Imaging
Cavities, eye 0,94 3,5 30
50/70at
488/660 325
SSeries
MSeries
S-300*
S-0650*
S-1500
UltraMiniO
MiniO/30
MiniO/100
Z-1800
Mini-Z
ZSeries
CerboFlex™CerboFlex™ J
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-20
12
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
Applicative Outcomes
Real-time videos acquired on a tumor xenograft implanted on a nude mouse
Cellvizio Dual Band provides unambiguous coregistered signal from two different fluorochromes.
FITC Dextran was injected as a vessel indicator, combined with AngioStamp®, a molecular marker that targets specificly AvB3 integrin, an endothelial pattern overexpressed in tumor neovessels.
Examples of early results obtained in vivo illustrated on the following figures :
Access to both functional and anatomical information in vivo
> Imaging of vessels and macrophages
Monitoring of the excretion process into the kidney
> Glomeruli and convoluted tubules visualized
CONFIDENTIAL - NOT FOR DISTRIBUTION
3 hours after injection, renal elimination can be appreciated- FITC Dextran (Green)- AngioStamp® (Red)
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-20
12
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
In Vivo Molecular Imaging at Cellular Resolution
www.maunakeatech.com
Image neurons at work, anywhere in the brainwith
EUROPEMauna Kea Technologies SA
9 rue d’Enghien,75010 Paris, France
Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18
USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889
STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons
HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons
CORTEXS-1500, Mini-Z,FITC injection,Microcirculation
SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™
ACCUBENSNeuroPak™, S-300,S-650, Addiction
VTAS-300, S-650, NeuroPak™
Lentivirus-transfectedneurons and individual
dendrites
VISUAL CORTEXS-650, Mini-Z, Oregon Green,
BAPTA-1, Neuron activation
Ver
sio
n 0
8-2
012
flyer-Neuro corrections (2.indd 1 22/08/12 16:50
Courtesy of F. Duconge, CEA, Orsay, France