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American Journal of Research Communication www.usa-journals.com In Vitro  Antioxidant, Cytotoxic, Thrombolytic,  Antimicrobial and Membrane Stabilizing  Activities of  Murraya  paniculata Tahmina  Akter Mita 1 , Mahbubul Hoque Shihan 1 , Mehreen Rahman 1 , Tasnuva Sharmin 1 , Mahfuza Maleque 1 , Mohammad RaziUlHasan  Alvi 2 and  Sharmin Reza Chowdhury 1* h. 1 Department of Pharmacy, State University of Bangladesh, Dhaka1205, Banglades 2  D epartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka 1000, Bangladesh. *Correspondence to: Sharmin Reza Chowdhury, Ass istant Professor, Depatment  of Pharmacy, State University of Bangladesh. Email: [email protected] .  ABSTRACT The methanolic extract of leaves of Murraya  paniculata and its petroleum ether, carbon tetrachloride, chloroform, and aqueous soluble partitionates were subjected to total phenolic content determination, free radical scavenging activity determination (DPPH assay), cytotoxic, thrombolytic, antimicrobial and membrane stabilizing activity assays. The antioxidant potential was determined in terms of total phenolic content and free radical scavenging activity assay. The highest free radical scavenging potential was demonstrated by the carbon tetrachloride soluble fraction (IC 50 = 72.51±0.69 g/ml) which could be correlated to its phenolic content (52.99±0.32mg of GAE / gm of extractives). In brine shrimp lethality bioassay, the pet ether soluble extractives and the crude methanol extract revealed the highest cytotoxic activity having LC 50  values 0.471±0.72 g/ml and 0.773±0.19 g/ml, respectively. In thrombolytic activity study, the crude methanolic extract exhibited mild clot lysis activity (27.94±0.23 %) compared to the standard streptokinase (66.77%). In antimicrobial investigation by disc diffusion method, none of Mita, et al ., 2013: Vol 1(5) [email protected] 226

In Vitro Antioxidant, Cytotoxic, Thrombolytic, Antimicrobial and Membrane Stabilizing Activities of Murraya paniculata

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