IMPACT PRP – Platelet Rich Plasma

Natural healing cascadeIf an injury occurs in the body, it reacts with a complex healing cascade in order to heal the wound as quickly as possible. At the site of the defect, the thrombocytes aggre-gate and form the blood clot. In addition, growth factors are released by the degran-ulation of thrombocytes. They act synergistically and favor the regeneration process.

Various PRP formulationsBy using IMPACT, various PRP formulations can be produced. For IMPACT, two PRPs have been developed, tested and optimized for certain results:rPRP: A “red” PRP which has been optimized for a very high platelet yield. It contains a signifi cant share of leukocytes and up to 7 percent of erythrocytes.wPRP: A “white” PRP which has been optimized for a high platelet yield under the condition of a very low content of leukocytes and the absence of erythrocytes.

wPRP – platelet suspension in plasmaThe IMPACT wPRP is a “white” PRP, that is, free from erythrocytes with a very low content of leukocytes. The platelet concentration is about two to three times above the physiological situation in whole blood (concentration depending on the selected end volume).

Growth factors foster natural regenerationThere is a direct linear correlation between platelet concentration and growth factors. Platelets have to be activated so that they can release growth factors. IMPACT wPRP has been developed in such a way that the platelets are activated minimally only by short and gentle centrifugation. The activation takes place in the body at the site of the defect. There, the growth factors are released and act chemotactically as well as directly and indirectly regenerative.

The high platelet yield in combination with a rather low concentration of growth factors in wPRP indicates the low activation condition of the platelets. This is the explicit ob-jective of IMPACT wPRP. For a higher concentration of growth factors before the injec-tion, the user can choose IMPACT APC or they can determine and save an individual program variant.

(1) Serum concentrations: TU Munich, Institute of Medical and Polymer Engineering

P l a t f o r m f o r A u t o l o g o u s C e l l T h e r a p i e s

Concentration compared to whole blood 1 Yield

Types of cell

Platelets Leukocytes Erythrocytes Platelets

wPRP 2–3 × < 0.2 × < 0.01 × 92%

rPRP 2–3 × < 0.9 × < 0.15 × 95%

Chemotaxis – attraction of undifferentiated mesenchymal stem cells

Cell division / proliferation

Cell differentiation

Formation of the fi brin mesh (”Clot”)

Emission of growth factors

Formation of the fi brin mesh

Cell division / proliferation

IMPACT wPRP – exemplary indications

ORTHOPAEDICS (acute and chronic) • Tendon injuries, e.g. Achilles tendon, rotator cuff • Ligament injuries, such as cruciate ligament, ligaments of ankle joint • Torn muscle fibers, meniscus injury

• Tendinopathy, such as Achilles tendon thickening / chronic Achillodynia • Tendonitis, such as patella-tendon syndrome • Tenosynovitis • Subacromial syndrome

DERMATOLOGY / AESTHETIC MEDICINE • Skin rejuvenation • (Androgenic) alopecia • Scars

P l a t f o r m f o r A u t o l o g o u s C e l l T h e r a p i e s

LITERATURE SELECTION Lynch MD, Bashir S (2016): Applications of platelet-rich plasma in dermatology: A critical appraisal of the literature. J Dermatolog Treat. 2016;27(3):285-9.

Singh B, Goldberg LJ (2016): Autologous Platelet-Rich Plasma for the Treatment of Pattern Hair Loss. Am J Clin Dermatol. 2016 Aug;17(4):359-67.

Jeong D et al (2014): Clinical applications of platelet-rich plasma in patellar tendinopathy; Biomed Res Int. 2014: 249498

Martinelli N et al (2013): Platelet-rich plasma injections for chronic plantar fasciitis; Int Orthopaedics, 2013, 37(5): 839-42

Cho JW, Kim SA, Lee KS (2012): Platelet-rich plasma induces increased expression of G1 cell cycle regulators, type I collagen, and matrix metalloprotein-ase-1 in human skin fibroblasts. Int J Mol Med. 2012 Jan;29(1):32-6.

Deans VM et al (2012): A prospective series of patients with chronic Achilles tendinopathy treated with autologous conditioned plasma injections combined with exercise and therapeutic ultrasonography; J Foot Ankle Surg, 2012; 51(6): 706-10

Li ZJ, Choi HI et al (2012): Autologous platelet-rich plasma: a potential therapeutic tool for promoting hair growth. Dermatol Surg. 2012 Jul;38(7 Pt 1):1040-6.

Mazzocca A et al (2012): The positive effects of different platelet-rich plasma methods on human muscle, bone, and tendon cells; Am J Sports Med, 2012, 40(8):1742-9

Milano G et al (2012): Repeated platelet concentrate injections enhance reparative response of microfractures in the treatment of chondral defects of the knee: an experimental study in an animal model; Arthroscopy, 2012, 28(5): 688-701