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ImageQuant™ TL (also called IQTL) is an advanced analysis software designed specifically for blots and 1D gel image analysis and quantification. IQTL Ver. 10 contains new and improved features:

Key benefits of ImageQuant TL

• Supports wide range of image file formats such as TIF, JPG, PNG, IMG, and GEL

• 3D view of images

• Easy multichannel image analysis

• Editing and contrast adjustment options at every stage of analysis

• Total protein normalization data with a single click

Amersham ImageQuant TL analysis software

• Lane comparison to similarity score scatter plots and dendrograms

• Customizable report generation

• Analysis toolbox module for analysis of any area of interest

• Available for both Windows and Apple computers

• GxP version for regulated environments requiring data integrity, user groups, and approval steps for 21 CFR Part 11 compliance support

ImageQuant TL analysis software is designed to be fully compatible with the Amersham™ range of ImageQuant 800 CCD imagers and Typhoon™ laser scanners enabling you to work efficiently and achieve the best results from your image data.

IMAGING SYSTEMS, SOFTWARE, AND ACCESSORIES

Navigation panel with analysis steps

Large image display working screen Settings panel to set parameters for analysis

Instructions panel

Fig 1. Streamlined ImageQuant TL user interface

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Intuitive user interface

The software user interface is divided into three sections (Fig 1):

• A navigation panel with quick access to image analysis steps

• Large, central view of your analyzed image, along with other analysis-dependent data like 3D views, lane profiles, and more

• A settings panel where you can easily adjust analysis steps, and an instructions panel with information on each step

Lane profile comparison Scientists often compare samples visually across different lanes, but this can be difficult, for example if samples have many bands. Visual examination is also time-consuming and may be biased. We therefore designed the IQTL lane profile comparison tool to analyze lanes and determine their similarity. This analysis tool will quickly give you an overview of all samples, automatically compare all lanes to a selected reference lane, and calculate a dendrogram similarity tree that groups all samples based on the calculated similarity score.

The lane profile comparison tool plots a similarity score against relative volume compared a reference lane. This correlation calculation helps you to quickly determine how similar the samples are without any bias. The resulting similarity scatter plot provides an overview of all lanes, both in terms of chemical similarity (the separation profile) and also in terms of amount. Furthermore, based on all possible pair-wise comparisons, it is possible to construct a relationship tree, a dendrogram, of all the samples in all the lanes. This grouping of samples is based on lane profile similarity, thus it corresponds to how similar samples are chemically in the electrophoresis steps. The software displays this dendrogram and the results table it is based on, for easy export and reporting.

You can use lane profile comparison to:

• Evaluate purified samples during manufacturing of biopharmaceuticals.

• Determine sample purity for batch release of drugs.

• Select a specific fraction from a chromatography run by comparing lanes of different fractions.

• Quickly identify artifacts or odd spikes in lanes.

• Analyze complex samples like cell lysates (Fig. 6).

• Speed-up gel shift assays and DNA fragment analysis.

Fig 3. Different background subtraction methods including rolling ball, rubber band, and constant value.

Fig 4. Automatic band detection with options to edit bands manually as needed.

Fig 2. Effortless lane detection, along with annotation and manual lane editing if needed.

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Normalization functionThe normalization function provides you with several ways to normalize the calculated values of band volumes. You can normalize to a single band or lane and to total protein and housekeeping proteins for multiplex images.

• In-channel band method: Normalizes all bands against a single chosen reference band. Single channel only.

• Multi-channel band method: Normalize band volumes based on bands such as housekeeping bands in each lane.

• Total protein method: Uses the total lane volume of all bands in the lane of a selected reference channel to normalize the bands in the same lane of other channels. Fig 7. shows total protein normalization for multiplex images.

Fig 5. The lane profile comparison tool automatically performs a full quantitative comparison of different lane profiles based on profile shape and peak volumes. These parameters reflect chemical similarity and amount of sample in the lane. The analysis is based on correlation of lane profiles and comparing lane volumes. The user can select to display: The image (A), selected lane profiles (B), all pair-wise profile correlations in the similarity table (C), and a scatter plot (D). The scatter plot shows the calculated similarity score for a selected reference lane. Users can select the reference lanes in the table, or in the dropdown list. Lane alignment can either be based on the lane area or on the Mw calibration if available (E). The software calculates and presents lane grouping in dendrogram form (F).

A C E

B D

F

Fig 6. Using the compare tool, similarity analysis grouped lysate samples and revealed similarity between sample groups. For example, the K1 cell lysate and supernatant lysate were similar and closely related.

Fig 7. Total protein normalization is easily done for multichannel images in IQTL 10. The total lane volumes of a selected reference channel, are used for normalization. In this example, the samples were labeled with Cy3 and probed for GAPDH and ERK proteins in a Western blot experiment.

CHO S Supernatant

CHO K1 Lysate

CHO K1 Supernatant

CHO DG44 Lysate

CHO S Lysate

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ImageQuant TL GxP module for full data integrityQuality guidelines and regulations check whether pharmaceutical products are safe for their intended use, and that the manufacturing, control, storage, and distribution processes adhere to documented quality processes. GxP is an abbreviation for good practice — “x” can stand for manufacturing or lab. GxP regulations describe requirements and guidelines on how you can use standard protocols for safe electronic record keeping.

GxP focuses on traceability, accountability, and data integrity. The ImageQuant TL GxP module along with the Amersham ImageQuant 800 GxP CCD imager help support compliance with FDA 21 CFR part 11 and EU GMP Annex 11.

ImageQuant TL GxP provides the data security you need in a regulated environment. Achieve full control of 1D gel electrophoresis and Western blot data with these features:

• Robust password control which can be configured to follow the required password policy

• User groups with different access levels based on role

• Audit trails with complete user activity, login attempt, and detailed analysis logs

• Client-server based data protection for safe archiving of data in a secure folder (Fig. 10)

• Electronic signatures for approvers with check-in and check-out process for data archiving and approval

• Emergency login capability

• Images generated by ImageQuant 800 GxP check image authenticity automatically before analysis

Analysis toolbox moduleThe analysis toolbox module provides many tools for analyzing images. These tools are especially useful when analysis requires manual editing of the region of analysis, for example to calculate signal-to-noise ratios. This module is also needed to analyze images with samples of various shapes, for example tissue sections. You can use the drawing tools to define and edit features in the image. The profile window displays intensity profiles along the lines and shapes you define. The software calculates volume and area (i.e., number of pixels and total pixel intensity), with or without subtraction of background intensity.

Drawing tools included are:

• Line

• Polyline (a line composed of multiple segments)

• Curve

• Rectangle

• Ellipse

• Polygon

Fig 8. Line drawing tool in the analysis toolbox module.

21 CFR Part 11

Compliance

Supports

EU GMPAnnex 11

Supports

Compliance

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Fig 9. ImageQuant TL GxP organizes image files by project. Users can then see images waiting for approval and sign off from lab managers.

Fig 10. Client-server data archiving process in ImageQuant TL GxP.

Check-in

Analysis completedAnalysis report

Image Analysis

Check-out

Add files and create project

Safe server storage

IQTL 1D Analysis

System requirements

Microsoft Windows

Windows® 10 (64 bit)

Processor: 1.2 GHz or better

Microsoft .NET Core 3.1

Apple

Mac® OS X® 10.14 or higher

Ordering informationImageQuant TL

Description Product code

IQTL 10 Node locked license 29655283

IQTL 10 MAC OS Node locked license 29656650

IQTL 10 MAC OS Node locked license (5 pack) 29656651

IQTL 10 Node locked license (5 pack) 29655284

IQTL 10 Floating license 29655286

IQTL 10 Floating license (5 pack) 29655287

IQTL 10 GxP Node locked license 29655289

IQTL 10 GxP Node locked license (5 pack) 29655290

IQTL 10 GxP Floating license 29655291

IQTL 10 GxP Floating license (5 pack) 29655292

IQTL 10 and IQTL 10 GxP Software Set 29655282

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Amersham and ImageQuant are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.

Apple is a trademark is a trademark of Apple Incorporated. Windows is a trademark of Microsoft Corporation.

All other third-party trademarks are the property of their respective owners.

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All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

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