Ikkitousen Quang Truong

Josh GarciaBIOL 1750.501

12/02/13

Collection Site Data

Quang Truong discovered the soil in the backyard of his home that he dug up from under his Japanese Maple Tree. The soil was moist and measured at 98 degrees Fahrenheit at 3:16 pm on July 22,2013.

32.609463 N

97.042534 W

Tree

Overview Phage Collection/

Morphology Size/ Measurements of Capsid – 63.713 nm

Tail - 241.658 nm

Head to Tail – 306.691 nm

Morphotype – Siphoviridae (Capsid diameter about 60 nm)

Ex: Adephagia, Denton TX, 2009,

Electron Microscopy (EM)

Electron Microscopy (EM)

DNA Yield/ Quality Concentration of DNA: 226.5 ng/ul

Current Volume as of 11/18/13: 65.2 ul

Current Total ug of DNA as of 11/18/13: 14.7678 ug

Phage Titer of HTL: 8.0 x 10^9 pfu/ml

RE/ Cluster/ Genome Size

Genome Size: N/A

Cluster: K (Due to similarities in comparison to Adephagia, Angelica, Emerson, etc.

LadderCut 1- 212

Cut 2- 229.333

Cut 3- 254.667

Cut 4- 266.667

Cut 5- 294.667

Cut 6- 338.667

Cut 7- 390.667

Cut 8- 438.667

Cut 9- 506.667

Cut 10- 613.333

150 200 250 300 350 400 450 500 550 600 6500

0.5

1

1.5

2

2.5

3

3.5

4

4.5

f(x) = − 0.00314405465620237 x + 4.57822107613621

Ladder

Series1Linear (Series1)

Discussion/Comparison &

Cluster ID- We narrowed down our phage to fall within Cluster K using the (PET) website.

- After determining our cluster, we were able to narrow down a sub-cluster in which we determined to be K1.

- Examples: Adephagia, Angelica, Emerson, & Amelie.

- In comparison to the other four clusters that fall under cluster K, we found that a few of the other Restriction Digestions had cuts under “ClaI” in the K4 Sub-cluster.

- Cuts of “BamHI,” & “HindIII” under K5 Sub-cluster.