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Identification of new sources of partial resistance to oat crown rust. Marty Carson, USDA Cereal Disease Lab, St. Paul, MN. Oat Crown Rust: the Pathosystem from Hell. 90+ Pc genes described; virulence to all of them Sexually recombining pathogen Positive virulence associations - PowerPoint PPT Presentation
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Identification of new Identification of new sources of partial sources of partial resistance to oat resistance to oat
crown rustcrown rust
Marty Carson, USDA Cereal Marty Carson, USDA Cereal Disease Lab, St. Paul, MNDisease Lab, St. Paul, MN
Oat Crown Rust: the Oat Crown Rust: the Pathosystem from HellPathosystem from Hell
90+ Pc genes described; virulence to all of 90+ Pc genes described; virulence to all of themthem
Sexually recombining pathogenSexually recombining pathogen Positive virulence associationsPositive virulence associations No apparent cost to complex virulenceNo apparent cost to complex virulence Resistant cultivars have a 3-5 yr lifespanResistant cultivars have a 3-5 yr lifespan
Insanity: continuing to do the Insanity: continuing to do the same thing, but expecting same thing, but expecting
different resultsdifferent results
‘‘Slow Rusting’ or Partial Slow Rusting’ or Partial Resistance to Oat Crown RustResistance to Oat Crown Rust Portage, Minhafer, Lodi (Heagle & Moore, Portage, Minhafer, Lodi (Heagle & Moore,
1970)1970) Red Rustproof (Luke et al, 1972)Red Rustproof (Luke et al, 1972) MN841801, MN841810, MN841804, etc. MN841801, MN841810, MN841804, etc.
(Leonard, 2002) (Leonard, 2002)
Problem: Identification of partial, non-specific Problem: Identification of partial, non-specific resistance in the presence of race-specific resistance in the presence of race-specific
resistance genes when corresponding resistance genes when corresponding virulence in the pathogen population is at a virulence in the pathogen population is at a
low frequency (Parlevliet, 1983)low frequency (Parlevliet, 1983)
Entry 2005 Buckthorn Nursery 2005 Virulent Inoculum
7/5 7/13 7/11 7/18
CDC Boyer 4S 45S 10S 25S
MN841801 2MR/MS 22MR/MS 7S 15MR
Pc68 Trace 7S 55S dead
GS7 7S 50S 7S 22S
IA 98822-2 5S 50S 20S 32S
CI4706 0 10S Trace 1S
CI5562 4S 50S 10S mature
CI5632 10S 60S 40S 40S
CI7035 0 20S 5S 10S
CI8220 10S 80S 25S 45S
CI8261 5S 50S 25S mature
CI8262 4S 55S 15S 30S
CI8429 Trace 30MR/MS 5MS 10S
CI9244 15S 80S 50S dead
CI9248 1S 10MR/MS 5MS 5MR
CI9416 4S 40S 15S 30S
PI189733 7S 30MS/MR 25MS 30MR/MS
PI260616 4S 30S 10MS 10MS
PI263412 Trace 20S 1MS 5S
PI266884 0 7S 1MR 3MR
PI266887 Trace 15S 2S 7S
Entry 2005 Buckthorn Nursery 2005 Virulent Inoculum
7/5 7/13 7/11 7/18
CDC Boyer 4S 45S 10S 25S
MN841801 2MR/MS 22MR/MS 7S 15MR
Pc68 Trace 7S 55S dead
GS7 7S 50S 7S 22S
IA 98822-2 5S 50S 20S 32S
CI4706 0 10S Trace 1S
CI5562 4S 50S 10S mature
CI5632 10S 60S 40S 40S
CI7035 0 20S 5S 10S
CI8220 10S 80S 25S 45S
CI8261 5S 50S 25S mature
CI8262 4S 55S 15S 30S
CI8429 Trace 30MR/MS 5MS 10S
CI9244 15S 80S 50S dead
CI9248 1S 10MR/MS 5MS 5MR
CI9416 4S 40S 15S 30S
PI189733 7S 30MS/MR 25MS 30MR/MS
PI260616 4S 30S 10MS 10MS
PI263412 Trace 20S 1MS 5S
PI266884 0 7S 1MR 3MR
PI266887 Trace 15S 2S 7S
Entry 2005 Buckthorn Nursery 2005 Virulent Inoculum
7/5 7/13 7/11 7/18
CDC Boyer 4S 45S 10S 25S
MN841801 2MR/MS 22MR/MS 7S 15MR
Pc68 Trace 7S 55S dead
GS7 7S 50S 7S 22S
IA 98822-2 5S 50S 20S 32S
CI4706 0 10S Trace 1S
CI5562 4S 50S 10S mature
CI5632 10S 60S 40S 40S
CI7035 0 20S 5S 10S
CI8220 10S 80S 25S 45S
CI8261 5S 50S 25S mature
CI8262 4S 55S 15S 30S
CI8429 Trace 30MR/MS 5MS 10S
CI9244 15S 80S 50S dead
CI9248 1S 10MR/MS 5MS 5MR
CI9416 4S 40S 15S 30S
PI189733 7S 30MS/MR 25MS 30MR/MS
PI260616 4S 30S 10MS 10MS
PI263412 Trace 20S 1MS 5S
PI266884 0 7S 1MR 3MR
PI266887 Trace 15S 2S 7S
Entry 2005 Buckthorn Nursery 2005 Virulent Inoculum
7/5 7/13 7/11 7/18
CDC Boyer 4S 45S 10S 25S
MN841801 2MR/MS 22MR/MS 7S 15MR
Pc68 Trace 7S 55S dead
GS7 7S 50S 7S 22S
IA 98822-2 5S 50S 20S 32S
CI4706 0 10S Trace 1S
CI5562 4S 50S 10S mature
CI5632 10S 60S 40S 40S
CI7035 0 20S 5S 10S
CI8220 10S 80S 25S 45S
CI8261 5S 50S 25S mature
CI8262 4S 55S 15S 30S
CI8429 Trace 30MR/MS 5MS 10S
CI9244 15S 80S 50S dead
CI9248 1S 10MR/MS 5MS 5MR
CI9416 4S 40S 15S 30S
PI189733 7S 30MS/MR 25MS 30MR/MS
PI260616 4S 30S 10MS 10MS
PI263412 Trace 20S 1MS 5S
PI266884 0 7S 1MR 3MR
PI266887 Trace 15S 2S 7S
Entry 2005 Buckthorn Nursery 2005 Virulent Inoculum
7/5 7/13 7/11 7/18
CDC Boyer 4S 45S 10S 25S
MN841801 2MR/MS 22MR/MS 7S 15MR
Pc68 Trace 7S 55S dead
GS7 7S 50S 7S 22S
IA 98822-2 5S 50S 20S 32S
CI4706 0 10S Trace 1S
CI5562 4S 50S 10S mature
CI5632 10S 60S 40S 40S
CI7035 0 20S 5S 10S
CI8220 10S 80S 25S 45S
CI8261 5S 50S 25S mature
CI8262 4S 55S 15S 30S
CI8429 Trace 30MR/MS 5MS 10S
CI9244 15S 80S 50S dead
CI9248 1S 10MR/MS 5MS 5MR
CI9416 4S 40S 15S 30S
PI189733 7S 30MS/MR 25MS 30MR/MS
PI260616 4S 30S 10MS 10MS
PI263412 Trace 20S 1MS 5S
PI266884 0 7S 1MR 3MR
PI266887 Trace 15S 2S 7S
Entry 2006 Buckthorn Nursery2006 Buckthorn Nursery 2006 Virulent Inoculum (1-9)2006 Virulent Inoculum (1-9)
6/28 7/5 7/14 7/23
CDC BoyerCDC Boyer 5S5S 30S30S 6.56.5 4.54.5
MN841801MN841801 5MS5MS 20MR/MS20MR/MS 7.57.5 5.55.5
Pc68Pc68 5S5S 30S30S 6.06.0 1.51.5
StarterStarter 30S30S 80S80S 3.03.0 1.01.0
CI1712CI1712 3MS3MS 15MR/MS15MR/MS 8.08.0 7.07.0
CI1965CI1965 4MS4MS 25MS25MS 6.06.0 3.53.5
CI4736CI4736 3S3S 10MR/MS10MR/MS 7.57.5 3.53.5
CI6991CI6991 5MS/S5MS/S 20S20S 6.56.5 3.03.0
CI8000CI8000 3MS3MS 15MS15MS 7.57.5 6.56.5
CI8357CI8357 5S5S 20MS20MS 8.08.0 4.54.5
CI8358CI8358 40S40S 65S65S 6.06.0 2.52.5
CI8432CI8432 5S5S 15MS15MS 6.56.5 3.03.0
CI9247CI9247 0R0R 4MR4MR 8.08.0 7.07.0
Entry 2006 Buckthorn Nursery2006 Buckthorn Nursery 2006 Virulent Inoculum (1-9)2006 Virulent Inoculum (1-9)
6/28 7/5 7/14 7/23
CDC BoyerCDC Boyer 5S5S 30S30S 6.56.5 4.54.5
MN841801MN841801 5MS5MS 20MR/MS20MR/MS 7.57.5 5.55.5
Pc68Pc68 5S5S 30S30S 6.06.0 1.51.5
StarterStarter 30S30S 80S80S 3.03.0 1.01.0
CI1712CI1712 3MS3MS 15MR/MS15MR/MS 8.08.0 7.07.0
CI1965CI1965 4MS4MS 25MS25MS 6.06.0 3.53.5
CI4736CI4736 3S3S 10MR/MS10MR/MS 7.57.5 3.53.5
CI6991CI6991 5MS/S5MS/S 20S20S 6.56.5 3.03.0
CI8000CI8000 3MS3MS 15MS15MS 7.57.5 6.56.5
CI8357CI8357 5S5S 20MS20MS 8.08.0 4.54.5
CI8358CI8358 40S40S 65S65S 6.06.0 2.52.5
CI8432CI8432 5S5S 15MS15MS 6.56.5 3.03.0
CI9247CI9247 0R0R 4MR4MR 8.08.0 7.07.0
Entry 2006 Buckthorn Nursery2006 Buckthorn Nursery 2006 Virulent Inoculum (1-9)2006 Virulent Inoculum (1-9)
6/28 7/5 7/14 7/23
CDC BoyerCDC Boyer 5S5S 30S30S 6.56.5 4.54.5
MN841801MN841801 5MS5MS 20MR/MS20MR/MS 7.57.5 5.55.5
Pc68Pc68 5S5S 30S30S 6.06.0 1.51.5
StarterStarter 30S30S 80S80S 3.03.0 1.01.0
CI1712CI1712 3MS3MS 15MR/MS15MR/MS 8.08.0 7.07.0
CI1965CI1965 4MS4MS 25MS25MS 6.06.0 3.53.5
CI4736CI4736 3S3S 10MR/MS10MR/MS 7.57.5 3.53.5
CI6991CI6991 5MS/S5MS/S 20S20S 6.56.5 3.03.0
CI8000CI8000 3MS3MS 15MS15MS 7.57.5 6.56.5
CI8357CI8357 5S5S 20MS20MS 8.08.0 4.54.5
CI8358CI8358 40S40S 65S65S 6.06.0 2.52.5
CI8432CI8432 5S5S 15MS15MS 6.56.5 3.03.0
CI9247CI9247 0R0R 4MR4MR 8.08.0 7.07.0
Entry 2006 Buckthorn Nursery2006 Buckthorn Nursery 2006 Virulent Inoculum (1-9)2006 Virulent Inoculum (1-9)
6/28 7/5 7/14 7/23
CDC BoyerCDC Boyer 5S5S 30S30S 6.56.5 4.54.5
MN841801MN841801 5MS5MS 20MR/MS20MR/MS 7.57.5 5.55.5
Pc68Pc68 5S5S 30S30S 6.06.0 1.51.5
StarterStarter 30S30S 80S80S 3.03.0 1.01.0
CI1712CI1712 3MS3MS 15MR/MS15MR/MS 8.08.0 7.07.0
CI1965CI1965 4MS4MS 25MS25MS 6.06.0 3.53.5
CI4736CI4736 3S3S 10MR/MS10MR/MS 7.57.5 3.53.5
CI6991CI6991 5MS/S5MS/S 20S20S 6.56.5 3.03.0
CI8000CI8000 3MS3MS 15MS15MS 7.57.5 6.56.5
CI8357CI8357 5S5S 20MS20MS 8.08.0 4.54.5
CI8358CI8358 40S40S 65S65S 6.06.0 2.52.5
CI8432CI8432 5S5S 15MS15MS 6.56.5 3.03.0
CI9247CI9247 0R0R 4MR4MR 8.08.0 7.07.0
What are these sources of Partial What are these sources of Partial Resistance?Resistance?
CI1712 (Siberian sel.; Canada)CI1712 (Siberian sel.; Canada) CI4706 (La Prevision, Argentina)CI4706 (La Prevision, Argentina) CI7035 (Buck sel., Argentina)CI7035 (Buck sel., Argentina) CI8000 (Ark. 2-51-20)CI8000 (Ark. 2-51-20) CI8357 (Fla. 65Q1896-97)CI8357 (Fla. 65Q1896-97) CI8429 (66Ab599, Fla.)CI8429 (66Ab599, Fla.) CI9247 (MN756515)CI9247 (MN756515) CI9248 (MN756555)CI9248 (MN756555)
PI189733 (Nacional 190, Ecuador)PI189733 (Nacional 190, Ecuador) PI260616 (Amarela 5282 B 32, Brazil)PI260616 (Amarela 5282 B 32, Brazil) PI263412 (Red Algerian #31, Algeria)PI263412 (Red Algerian #31, Algeria) PI266884 (Santa Maria, Portugal)PI266884 (Santa Maria, Portugal) PI266887 (Santa Rosa, Portugal)PI266887 (Santa Rosa, Portugal)
Frequency Distribution of F5
Families MN841810 X Stout - 2006
0
10
20
30
40
50
60
<2.00 2.01-3.00
3.01-4.00
4.01-5.00
5.01-6.00
6.01-7.00
7.01-8.00
>8.01
Disease Rating 7/17 (1-9)
# Families
Stout
MN841810
Frequency Distribution of F5
Families MN841801 X Stout -
0
10
20
30
40
50
60
70
80
<2.002.01-3.003.01-4.004.01-5.005.01-6.006.01-7.007.01-8.00
>8.01
Disease rating 7/17 (1-9)
# F
5 familiesStout
MN841801
Barriers to Using Partial Barriers to Using Partial Resistance in Oat BreedingResistance in Oat Breeding
Complex inheritanceComplex inheritance Poor Agronomics of SourcesPoor Agronomics of Sources BYDV SusceptibilityBYDV Susceptibility Associated with Late Maturity (Diaz-Lago Associated with Late Maturity (Diaz-Lago
et al, 2002)et al, 2002)
