pdswjt

psa

h

P

Cn

ÖDU

1

S2

33

V4

eT5

B

AtISDfrasbitaaa

S

h

P

S

HÖ

U

ADrdotAttoebra

h

P

I

N

D

IfCoLofiPbelbwai

S

In this study, we developed a diagnostic kit based on Com-etitive Enzyme Linked Immunoassay (ELISA) for qualitativeetermination of HBc total antibodies (IgG and IgM) in humanerum. For this system, rabbit polyclonal antibody against HBcAgas developed and labelled with horseradish peroxidase. This con-

ugate was competed with the human Anti-HBc total antibody forhe HBcAg coated on well.

Developed competitive ELISA system was tested by using 256atients’ sera. As compared with the other conventional kits, it washown that our system corresponded with the results of negativend positive samples at ratio of 98.5%.

ttp://dx.doi.org/10.1016/j.nbt.2012.08.645

oster 5.0.206

ultivable sponge-associated actinobacteria: Phyloge-etic diversity and antimicrobial activities

zlem Öner1, Güner Ekiz2, E. Esin Hames-Kocabas2,∗, Volkanemir3, Özkan Gübe1, F. Can Özkaya1, M. Baki Yokes4, Ataczel5, Erdal Bedir2,∗

Department of Biotechnology, Graduate School of Natural and Appliedciences 35100, Bornova, Izmir, TurkeyDepartment of Bioengineering, Faculty of Engineering, Ege University,5100 Bornova, Izmir, TurkeyInstitute of Marine Sciences and Management, Istanbul University,efa, 34116 Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Arts and Sci-nces, Halic University, Sıracevizler Cd. No:29 Bomonti, Sisli, Istanbul,urkeyDepartment of Biology, Faculty of Science, Ege University, 35100ornova, Izmir, Turkey

ctinobacteria is a member of the bacterial phylum has been iden-ified as one of the dominant microbial associates of the sponges.n this study, 16 marine sponge samples from East Mediterraneanea were used to isolation of sponge-associated actinobacteria.ifferent media and pre-treatment methods were implemented

or the isolation experiments. Twenty representative isolates theange of morphological diversity were investigated in term ofntimicrobial activity, seawater requirement and 16S rRNA geneequencing. The phylogenetic analysis showed that the isolateselonging to genera Streptomyces and Nocardiopsis and all of thesuıolates have not shown an absolute requirement for seawa-er. Fifteen of the 20 representative isolates have exhibited anntimicrobial activity against methicillin resistant Staphylococcusureus and/or vancomycin resistant Enterococcus faecium and 3 ofll against Candida albicans.

Keywords: Marine sponge, sponge-associated actinobacteria,treptomyces, Nocardiopsis, antimicrobial activity, 16S rRNA

ttp://dx.doi.org/10.1016/j.nbt.2012.08.646

laaioo

s

h

230 www.elsevier.com/locate/nbt

New Biotechnology · Volume 29S · September 2012

oster 5.0.207

creening of antibacterial effects of isolated microfungi

. Halil Biyik∗, Esin Poyrazoglu Coban, Gamze Basbülbülzdemir, Merve Yavuz, Yasemin Sertel

Faculty of Science and Letters, Biology Department, Adnan Menderesniversity, 09010 Aydin, Turkey

total of 252 isolated microfungi that registered stocks in Biyologyepartment of Adnan Menderes University were used as mate-

ial. Qualitative screening of antibiotic producing microfungi wasone by the method of Wickerham’s Spectrum Test Plates in antibi-tic test media. Ten different test strains were used to determinehe antimicrobial effects of microfungi in antibiotic test media.s a results of qualitative screening 13 isolated fungi were cul-

ured in 1 L of liquid Potato Dextrose Media, in conical flasks forhe small-scale extracts production. After growth period (10 days)n standing fermentation conditions, the fungal mycelia werexhaustively extracted from ethyl acetate (the fermentation fluidy 1/5 ethyl acetate was added). The collected liquid was evapo-ated at 77 ◦C. The antimicrobial activities of both the myceliumnd fermentation fluids examined by hole method.

Keywords: Fungi; Antibacterial effect

ttp://dx.doi.org/10.1016/j.nbt.2012.08.647

oster 5.0.208

dentification of biodiesel wastewater organisms

ursel Kıratlı Yılmazcoban∗, Ismail Ayhan Sengil, Meral Yurtsever

Sakarya University, Engineering Faculty, Environmental Engineeringepartment, Esentepe Campus, 54187 Sakarya, Turkey

n this study, complete mix activated sludge reactor designedor biological treatability of the extracted biodiesel wastewater.omplete mix activated sludge process was operated, continu-usly fed by extracted biodiesel wastewater with the flow of 25.82day−1, hydraulic retentation time 4 hour, dissolved oxygen levelf between 2–3 mg/L. Gram, Neisser and PHB stain was used andlament identification was carried out under the light microscope.reparates including filamentous microorganisms were identifiedy matching the filamentous bacteria identifications like in the lit-rature. Actinomycetes, S.natans and Type 0092 were continuouslyived in the environment up to the weekly examinations in theioreactor. Because of having small molecule organic componentsere being much in the environment, Actinomycetes and S.natansre in the environment. Because there is enough O2, N and P. Theres not enough knowledge about physiology of the Tip 0092 in theiterature. Type 0092 was independently existed between the waternd the flocks in the industrial plants. N.limicola II and Type 021 Nre known to be grown in the organic wastes. With these organ-sms which are dominant in the activated sludge with enoughxygen and nutrient existence, 87% rate of COD and 55% ratef TN removal were evaluated.

Keywords: Biodiesel; Wastewater; Oil extraction; Activatedludge; Identification

ttp://dx.doi.org/10.1016/j.nbt.2012.08.648