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MESSAGE FROM CHIEF PATRON

It is indeed a great pleasure to know that Department of Chemistry Arts,

Commerce and Science College, Satral is organizing a national seminar on “Green

Chemistry Education for a Sustainable Future of Humanity” (GCESFH-2016), 23rd &

24th December 2016 sponsored by Savitribai Phule Pune University Pune.

I, on behalf of Pravara Rural Education Society, Loni, welcome the scientists,

researchers, resource persons and participants for this National Seminar. I am

confident that this seminar will provide excellent opportunity for interaction among the

eminent scientists, academicians and young enthusiastic researchers to share their

knowledge and experience to achieve the objectives of the seminar.

I wish this seminar “Green Chemistry Education for a Sustainable Future of

Humanity” (GCESFH-2016), every success.

Dr. BALASAHEB VIKHE PATIL

(Padmabhushan-Awardee)

Former Union Minister for Heavy Industries (Govt. of India)

Chairman, Pravara Rural Education Society, Pravaranagar

MESSAGE FROM EXECUTIVE CHAIRMAN

I am glad to learn that the Department of Chemistry, Arts, Commerce & Science

College, Satral is organizing a two day national seminar on “Green Chemistry

Education for a Sustainable Future of Humanity” on 23rd & 24th December, 2016.

This seminar shall provide an excellent platform to the participants to showcase their

research and will also provide them an opportunity to enhance their knowledge in the

field of Green Chemistry.

It is very essential that the knowledge acquired by the scientist be shared with

the masses for development of the society. At Pravara Rural Education Society, we

have a strong belief that the quality of life of the farmer should be benefited by the

research findings.

We are sure that the delegates will inspire the participants by exchanging the

recent Scientific & Technological advances which will undoubtedly strengthen the

links among the scientific community.

This seminar true to its theme shall provide an impetus to the younger

generation & budding researchers to delve further into the realms of Green Chemistry

and do path breaking research which shall help in sustaining the future of Humanity.

I, on behalf of Pravara Rural Education Society extend a very warm welcome to

all the delegates & participants and wish them a very pleasant & memorable stay in

Pravara Campus. I also congratulate the organizers for selecting the appropriate

theme and convey my best wishes for the seminar.

DR. ASHOK VIKHE PATIL Executive Chairman

Pravara Rural Education Society, Pravaranagar

From the Principal’s Desk

It gives me an immense privilege and pleasure to welcome you all at this BCUD

Savitribai Phule Pune University, Pune sponsored National level Seminar on “Green

Chemistry Education for a Sustainable Future of Humanity” on behalf of Pravara Rural

Education Society’s Arts, Commerce and Science College, Satral. Since its inception

the Pravara Rural Education Society has made a remarkable achievement in

changing the socio-economic status in the rural masses through need and quality

based Higher Education. In reorganization of the excellent educational facilities

provided and services rendered to the rural community. Today the chemical

sciences are playing a significant role in expanding the horizons of science and

technology. In fact we had a very little span to organize such a big event the College

have taken sound efforts for the organization of this Seminar and lead to make

available open platform for researchers, scientists, academicians, industrialists and

policymakers to discuss the various issues and challenges before the globe.

It is with these sentiments we take over as the organizers from Pravara group of

institutions who for a last several decades has cleverly and effectively lead the

educational trust organizing the National Seminar on “Green Chemistry Education for a

Sustainable Future of Humanity” (GCESFH-2016) at ACS College, Satral, during

December, 23rd & 24th 2016. I am sure; this seminar will be helpful to inclusive

growth of the rural and underprivileged people of the society.

The deliberations to be held during the seminar will bring new insights and help

the researchers in the various fields to carry out the quality research. I am grateful

to those who directly or indirectly boosted us to organize this event. I convey my

best wishes for grand success of this seminar.

MAJ. R.S. SHINDE Principal & Convener

From the Head, Department of Chemistry

The department of chemistry was established in 1999 and PG Analytical

chemistry in 2008 for imparting knowledge in chemical sciences at under graduate

and post graduate level. The Department of Science and Technology ( DST) New

Delhi, has provided a grant of Rs. 39 lakhs under FIST programme and UGC Rs.

10 lakhs for imparting the infrastructure and sophisticated instruments facilities in

the department. The department is equipped with sophisticated instruments

facilities like Atomic Absorption Spectrophotometer, UV-Visible Spectrophotometer,

Potentiostat etc.

The strength of our department is reflected by research and placement

activity. Six Minor and one major research projects funded by UGC and Savitribai

Phule Pune University were completed and two minor projects are ongoing. The

department staff and PG students do their research work in the emerging fields of

chemistry related to co-ordination chemistry, separation sciences, synthetic organic

chemistry, green chemistry etc. More than 60 research papers have been published

by our staff in the journal of national and international repute.

Along with teaching and research activities, the department as a service to

society, conducts campus interviews. It is significant to note that more than 80% of

the students have secured employment in several pharmaceutical, chemical and

other industries. The activities of our department are focused on achieving the

mission of producing scientists, entrepreneurs and professionals with academic

knowledge and skills for contributing to the current scientific demand of the society.

Dr. G. R. PANDHARE

Associate Professor and Head

Department of Chemistry

ACKNOWLEDGMENT

It gives us a great pleasure to welcome all the dignitaries,delegates,invited

speakers, teachers and students to this national seminar “ Green Chemistry

Education For a Sustainable Future of Humanity”(GCESFH-2016). We are happy to

place this souvenir at the hands of experts and all participants. It carries program,

messages, abstracts, blessings and research articles.

We certainly hope that the speakers and all the participants will find the

seminar a thought providing and enjoyable experience .we are confident that the

seminar will be starting from the numerous collaborative efforts among the

academic institutions ,research areas and the pharmaceutical industries. The role

played by the department of chemistry for the upliftment of students community is

commendable .we are thankful to the resource person who are the facilitors and are

skilled eliciting the right answers from others.

We are deeply indebted to the Hon’ble Dr. Shri. Balasaheb Vikhe Patil,

(Padmabhushan Awardee) and Chairman, Hon’ble Shri. Radhakrishna Vikhe Patil,

Leader of Opposition, Maharashtra Legislative Assembly and Hon’ble Dr. Ashok

Vikhe Patil, Executive Chairman and all members of the management of Pravara

Rural Education Society, Pravaranagar for granting permission and providing

necessary facilities for the seminar.

We must express our sincere gratitude to BCUD, Savitribai Phule Pune

University, Pune for sponsoring such important event. We also thanks to all the

members of advisory board, scientific committee, chairpersons and members of

various committees, volunteers for their tireless efforts.

We are also thankful to all invited speakers, scientific session chairpersons, and coordinator, scientific oral and poster contributors, all the friends and colleagues without whose contribution it would not have been possible to organize this seminar. Thanks one and all.

Mr. P. L. HARALE Mr. D.S. AUTE Mr.V. A. KADNOR

ORGANIZERS

NATIONAL SEMINAR ON

“Green Chemistry Education for a Sustainable Future of Humanity”

(23rd

& 24th

December, 2016)

Patrons

Hon’ble Dr. Balasaheb Vikhe Patil, Pamabhushan Awardee & Former Union Minister Govt. of

India.

Hon’ble Dr. Ashok Vikhe Patil, Executive Chairman, Pravara Rural Education Society,

Pravaranagar, Maharashtra.

Hon’ble Shri Radhakrishna Vikhe Patil, Leader of Oppositions, Maharashtra Legislative

Assembly Govt. of Maharashtra.

Maj. General Neeraj Bali, CEO, PRES, Pravaranagar

Hon’ble Shri Bharat Ghogare Patil, Joint Secretary, PRES, Pravaranagar

Hon’ble Dr. Vasudeo Gade, Vice-Chancellor, Savitribai Phule Pune University, Pune

Advisory COMMITTEE

Prof.Dr. B.P.Bandgar, Solapur Dr. D.B. Shinde, Kolhapur

Dr. Y.H. Deshpande Aurangabad Dr. R. A. Mane Aurangabad

Dr. P. P. Wadgoankar, Pune Dr. S. P. Gejji, Pune

Dr. A.R. Saundane, Gulbarga Dr. Sanjay Batra, Lucknow

Dr. A.B. Devkhile, Aurangabad Dr. B.K. Karale, Ahmednagar

Dr. V. M. Shinde, Pune Dr. S. N. Shelke, Kopergaon

Dr. A.G. Durgude, Rahuri Dr. S S Borhade, Sangamner

Dr. S R Kuchekar, Pravaranagar Dr. A.S. Tambe, Rahata

Convener

Prof. Maj. R. S. Shinde, Principal ACS, College, Satral.

Coordinator

Dr. G.R. Pandhare, Associate Professor and Head, Department of Chemistry

Organizing secretaries

Mr. P.L. Harale, Assistant Professor, Department of Chemistry

Mr.V.A. Kadnor, Assistant Professor, Department of Chemistry

Treasurer

Mr.D.S. Aute, Assistant Professor, Department of Chemistry

WORKING COMMITTEE National Seminar on Green Chemistry Education for a Sustainable Future of Humanity

(23rd and 24th December 2016)

Sr. No. Name of the Committee Responsible persons 1. Steering & Organizing Committee Prof. Maj. R.S. Shinde.

Prof. Singar J.R. Dr. Pandhare G.R. Miss . Salunke A. C. Dr. Bhosale A. M. Dr. Tambe R. S. Prof. Kanhe N.S.

2. Registration

Prof. Pulate S. D. Prof. Tajane U.A Prof. Thete M.S. Mr.Tambe R.B.

3. Souvenir

Prof. Harale P.L. Prof. Kadnor V.A Dr. Bhosale A.M.

4. Break Fast tea/Coffee and Lunch

Prof. Kanhe N.S. Prof. Suryawanshi A.G. Prof. Shinde V.G. Dr. Palghadmal K.V. Prof. Ingale J.D.

5. Transport and Accommodation

Prof. Wani B.K. Mr.Aute D.S. Prof. Ghane D.N. Prof. Palghadmal V.S.

6. Stage & Seating Arrangement, Paper & Poster Presentation

Prof. Tambe R. S. Prof. Kedare A.N Prof. Yedke A.R. Prof. Pandure L.H. Miss. Rohmare S.S.

7. Rangoli and flower Boockey

Mrs. Agarkar D.D. Prof. Miss. Dighe S.G.

8. Report Writing & News

Dr. Kurhe A.R. Dr. Wadmare R.L. Prof. Kanhe N.S. Prof. Susar S.R. Prof. Agarkar D.D.

9. Invitation Distribution LMC members , PRES colleges

Prof. Shingote S.N. Prof. Mrs. Karle C.S. Prof. Kadaskar T.D. Prof. Dighe V.K.

10. Anchoring

Prof. Susar S.R. Prof. Borude S.N. Dr. Lamkhede S.R.

National Seminar on

Green Chemistry Education for a Sustainable Future of Humanity 23rd and 24th December 2016

Programme Schedule

Day and Date Time Program

Friday

23rd Dec. 2016

9.00am-10. 45am Registration and Breakfast

11.00am-11.50am Inaugural Function

11.50am-12.05pm Tea Break

12.05pm- 1.00pm Key note address

Dr. B.P. Bandgar

(Former VC, Solapur University,

Solapur)

1.00pm-2.00pm Lunch Break

2.00pm-2.45pm Plenary Lecture-I

Dr. R.A. Mane

Dr. BAM University, Aurangabad

2.45pm-3.30pm Plenary Lecture-II

Dr. Y.H. Deshpande

Dr. BAM University, Aurangabad

3.30pm-3.40pm Tea Break

3.40pm-4.40pm Oral Presentations

4.40pm-5.40pm Poster Presentation

Saturday

24th Dec. 2016

9.00 am -9.30 am Breakfast

9.30 am -10.15 am Plenary Lecture-III

Dr. A.G. Durgude

Mahatma Phule Krishi Vidyapeeth,

Rahuri

10.15 am -11.00 am Plenary Lecture-IV

Dr. D.D. Tayade

SGM University, Amravati

11.00am -11.15am Tea Break

11.15 am -11.45am Oral Presentations

11.45am -12.15pm Plenary Lecture-V

Dr. A.J. Aher Friedrich Alexander

University, Erlangen, Germany

12.15 pm -1.00 pm Poster Presentation

1.00pm-2.00pm Lunch Break

2.00pm-3.00pm Poster Presentation

3.00pm Valedictory Function

NATIONAL SEMINAR

National Seminar On

GREEN CHEMISTRY EDUCATION FOR A

SUSTAINABLE FUTURE OF HUMANITY

(GCESFH-2016)

23rd

& 24th

December 2016

INDEX

Sr No

Title Author Page

No.

1 One pot synthesis of 1-substituted-2-thio-6-

allylamino-4-[2-isobutoxy-5(4-methyl-5-

carboxy-1, 3-thiazo-2-yl)]-phenyl-1,3, 5-

triazines

A, S. Shendge, S. P.

Meshram, D.T.Tayade

01

2 Solvent Free Synthesis Of O-

Substitutedthiocarbamido-2-

Aminobenzothiazole By Microwave Irradiation

Technique

Rahim Ullah S, K.S.

Panpaliya, S. P.Chaudhari,

D.T.Tayade

05

3 A Novel Green Synthesis Of 1-Phenyl-3-[4-

(2,4-Dithio-3-T-Butyl-5 Substituted-1,3,5-

Triazino) Aminophenyl]-Prop-2-Ene-1-Ones

S.S. Padhen, D. T. Tayade,

D. A. Pund

09

4 Green Synthesis Of 8-[(2-Thio-3-Methyl-6-

Substitutedamino)-1,3,5-Thiadiazino]Imino-1-

Methyl-6-Phenyl-4h-[1,2,4] Triazolo [4,3-A]

[1,4] Benzodiazepines

P. R. Kale, D.T.Tayade, G.

D. Tayade

14

5 Green Synthesis Of 2-[-2-Hydroxy-5-(2,4-

Substituted-Dithiobiureto)] Phenylindoles

R. D. Isankar, D. T.

Tayade, G. G. Mule

16

6 One Pot Synthesis Of N’-(2-Hydroxyphenyl)-

N”-Substitutedthioureas

S. O. Mohod, D. T. Tayade,

G.G.Jadhav 20

7 Microwave assisted synthesis and anti-

microbial evaluation of some novel 2-

aminopyrimidines”

D. T. Tayade,

S.A.Waghmare

24

8 Green Methodology For Determination Of

Pharmacodynamics Of Chemical Molecules

A. B.Wadekar, D.T.Tayde 27

9 Plant Assisted Plant Assisted ZnO nanoparticle

as catalyst for efficient green one-pot synthesis

of benzimidazole derivatives .

Gurumeet C. Wadhawa,

Vitthal S. Shivankar ,

Yashwant A. Gaikwad,

Charansingh H Gill,

Laxman V. Gavali

31

10 Eco-friendly set up for determination of

stability constants of 4-(p-tolyl)thiocarbamido-

phenol complex of Cu(II), Cd(II), Co(II) and

Ni(II) metal ions in 70% ethanol-water system

N.J. Meshram, D.T.Tayade,

K. D.Tayade , G.D.Tayade

36

11 Spectoscopic analysis of some medicinal plants

with some infected blood samples

Supriya Kate and Harsha

Chathrath 37

12 Ultrasonic studies of 2-Aminothiazole at

different temperatures.

A.B. Naik, P. B .Morey ,

A.B.Bhagwatkar and S. U.

Patil

37

13 Sonochemical synthesis of La-doped ZnO

nanostructures and their photocatalytic activity

S. S. Hande, V.S.Astekar

S.P.Chaudhari,D.T.Tayade,

S.P.Meshram, P.D.Jolhe

38

14 Ultrasound Assisted Synthesis Of Isoxazolone

Derivatives Using Ionic Liquid As An Efficient

And Green Catalyst.

Gopinath D. Shirolea,

Adinath S. Tambe, Sharad

N. Shelke

39

15 Synthesis, Characterization and Biological

Activities of Macrocyclic Ligand.

S. A. Najan 39

16 Green Chemistry R. V. Laware, G. P. Kadu 40

17 Development of Spectroscopic method for the

determination of organic pollutants by Green

Analytical Techniques.

Vijay Bhusal, Kailas

Tambe , Vitthal Vikhe

41

18 Solar Photocatalytic Degradation Of

Rhodamine B Using Co-Doped Sno2 Supported

On Activated Carbon

S.R. Kande , U.G. Ghoshir

,

S.S. Jadhav, S.B. Pawar,

G.G. Muley, A.B. Gambhire

41

19 Utilization of honey as sweetener for the

preparation Of yoghurt drink

R.V. Darade , A. A.Walunj

P. B. Abhang 42

20 Removal Of Heavy Metals From Aqueous

Solution By Low Cost Adsorbent

Sureshkumar Halnor

42

21 H-NMP Catalysed Efficient Green Protocol for

the Synthesis of Pyrazolopyridines

Anil G. Gadhave ,Bhagwat

K. Uphade 43

22 Removal of crystal violet dye from aqueous

solution by calcinized eggshells

B. K. Uphade, A. V.

Borhade

43

23 [(Diacetoxyiodo) benzene] (DIB) catalyzed

three component one pot synthesis of 1, 8-

Acridinedione derivatives under solvent free

conditions

Amit S.Waghmare, Kailash

R. Kadam

44

24 Synthesis and Characterization of Mn (II) & Ni

(II) complexes of 4-chloro-2-[(E)-[1-(4-

fluorophenyl)-1H-pyrazol-4-

yl](hydroxyimino)methyl]phenol

Namdeo T. Dhokale,

Bausaheb K. Karale ,

Arvind V. Nagwade

45

25 Synthesis and biological evaluation for anti-

depressant activities of triazole substituted

phenothiazine derivatives.

Magar Sagar D, Dighe Amol

S, Dighe Nachiket S.

45

26 Studies on Total Lipid Content of Some Wild E

dible Fruits using Conventional and

Ultrasound Method

Vaishali J. Khilari, Pramod

P. Sharma and Somnath S.

Gholap

46

27 Synthesis And Biological Evaluation Of Some

Novel 2, 5 –Disubstituted [1, 3, 4]-Oxadiazole

Bearing 2, 2-Dimethyl-2, 3-Dihydrobenzofuran

Scaffold As Potential Anti-Tubercular Agents

Macchindra S. Tambe,

Laxman Nawale, Dhiman

D. Sarkar, Somnath S.

Gholap

47

28 Evaluation Isolation and Characterization of

Chemical constituents from C. bonducella L.

seed

SunayanaVikhe, Sunil

Nirmal.

48

29 Effect of L-Ascorbic Acid Supplementation on

Dicofol Induced Alteration in the Ascorbic

Acid Levels of an Experimental Model

Parreysia cylindrica

Ram S. Tambe

48

30 Removal of crystal violet dye from aqueous

solution by calcinized eggshells Development

and Validation of HPTLC Method for

Estimation of Gymnemic Acid in

Microencapsulated Antidiabetic Polyherbal

Formulations

Nirmal S.A, Vikhe D.N and

Dukre T.P

49

31 Exploitation of microbes to enhance crop

productivity- a sustainable approach

Durgude R. A. and V. S.

Supe 50

32 Aqueous Medium One Pot Synthesis of 2-

subsituted Benzimidazole”

Amruta K. Mhaske, Rani J.

Gaikwad, Vinod R. Kadu 50

33 Biosynthesis, Characterization and

antimicrobial activities of Nickel Nanoparticles

Using Ocimum sanctum (Tulsi) Leaf Extract

S. R. Kuchekar, H. R. Ahe

and P. M. Dighe

51

34 DABCO: An efficient and green catalyst for

one pot four-component synthesis of 11-amino-

12-(4-aryl)-8,9,10,12-tetrahydro-7H-

chromeno[2,3-b]quinolin-3-ol derivatives.

Akash D. Gholap and

Shivaji S. Pandit*.

51

35 Studies on Carbohydrate and Protein Contents o

f Some Underutilized Wild Fruits

Vaishali J. Khilari, Pramod

P. Sharma , Somnath S.

Gholap

52

36 Synthesis and biological Screening of some

Halogenated Chromones.

Vijay A. Kadnor, Gajanan

R. Pandhare, Sharad N.

Shelke

53

37 “Green Catalyst for effective Organic

Synthesis”

Vinod R. Kadu, Amol K.

Kharde, Somnath S. Gholap 53

38 Antitubercular activity of Thiophene and

Thiazole anchored flavones

B. K. Karale , S. J. Takate 54

39 Effect of Aluminium On Human Health Kanchan S. Deshmukh 55

40 Save Me From Global Warming C. S. Karle 55

41 Soil Health Card for Sustainable Crop

Production

A. G. Durgude

57

42 A review on Applications of Nanotechnology A.P. Londhe, B.R. Mhaske 59

43 Green Chemistry in Textile Industry

Anuradha G.Wandhekar 60

44 Synthesis and study on optical properties of Nd

doped calcium lanthanum borate glasses

Gajanan B. Harde, Gajanan

G. Muley 60

45 Study on effect of Nickel Doping on Optical

Properties of L-Arginine Phosphate Crystals

Gajanan G. Muley, Anil B.

Naik, Anil B. Gambhire,

Deepak T. Tayade

61

46 Development and Progress in Fiber Optic Urea

Biosensor

Sunil N. Botewad, Vikas G.

Pahurkar, Gajanan G.

Muley

61

47 Single Crystal Growth of Mg2Na2ZnB4O10

(MNZB) and Nonlinear optical study

S. R. Dagdale , G. G. Muley 62

48 Optical Fiber based Sensor for Ammonia

detection: A Review

Vishal D. Wankhade,

Gajanan B. Harde, Vikas G.

Pahurkar, Gajanan G.

62

Muley

49 Biochemical Changes Due To Application Of

Bioagent’s Innaculants Used For Biological

Management Of Root-Knot Nematode,

Meloidogyne Incognita Infesting Pomegranate

S. N.Varpe, A. R. Walunj,

N. L. Mhase

63

50 Evaluation of promising genotypes of

ber (Zizyphus mauritiana Lamark) against

its pest Complex.

R. E. Papade, A. R. walunj

63

51 Compatibility Of Insecticides With Fungicide

And Fertilizers On Pomegrnate

Ashok R. Walunj

Rahul Lad 64

52 Rapid Determination of tellurium(IV) by

Ultraviolet Spectrophotometry using o-

methylphenyl thiourea as a new chromogenic

ligand

Shashikant R. Kuchekar,

Shivaji D. Pulate

64

53 Knot Nematode, Meloidogyne Incognita Infesting

Pomegranate

S. N. Varpe, A. R. Walunj,

N. L. Mhase 65

54 Future perspective for formaldehyde pathways

for reductive synthesis and energy storage

Tambe Digambar C,

Kothule Revannath A,

Wabale Jaydip D.

65

55 Green Chemistry A Natural Evaluation

Initiative For Prevention Of Environment

Pollutions

Prashant L. Harale, Dilip S.

Aute, D.D.Agarkar

66

56 Green Alternatives for Organic Synthesis Kavita K. Raut, Amol K.

Kharde, Vinod R. Kadu 66

57 Synthesis of Bio-diesel from Vegetable oil Rani J. Gaikwad, Amruta K.

Mhaske 67

58 Green Chemistry: Tool for Healthy

Environment

Dubey Anuradha

Wandhekar Meenakshi 68

59 Applications of Green Chemistry Principles In

every Day life

Rohamare S.S, Dighe S.G.

Palghadmal V.S. 68

60 A study on phyto-chemical Screening and

antibacterial activity of Gymnema sylvestre

against pathogenic strains

Ranjit R. Raut,

Dnyaneshwar M. Shimbre

Ashok M. Bhosale , Anil R.

Kurhe

69

61 Know and Practice Nature’s Protocols for

Sustaining Future of Humanity: Biocatalysis

R. A. Mane

70

62 Silver Nanoparticles Sytnhesized under

Clinorotation and Assesment of their Cyto-

Genotoxicity

Avinash J. Aher, Pandit B.

Vidyasagar

71

63 Production of enzymes by

Bradyrhizobiumjaponicum strains

Kalpana Palghadmal, A. M.

Bhosale

72

ACS College Satral ISBN: 978-93-84659-81-3

Green Chemistry Education for a Sustainable Future of Humanity-2016 Page 1

One pot synthesis of 1-substituted-2-thio-6-allylamino-4-[2-isobutoxy-5(4-methyl-5-

carboxy-1, 3-thiazo-2-yl)]-phenyl-1,3, 5-triazines

A. S. Shendgea, S. P. Meshram

b, D.T.Tayade

a*

aDepartment of Chemistry, Government Vidarbha Institute of Science and Humanities, Amravati

444604.(MS) b Centre for materials for electronics technology (C-MET), Pashan, Pune- 411 008

*corresponding author [email protected], [email protected].

Abstract

Development of non-hazardous synthetic methodology is one of the greatest

challenges in synthetic chemistry. So such medium are replaced in green synthesis by making

use of non hazardous natural solvents like lemon juice, apple juice. One pot synthesis of non-

conventional synthetic method has been carried out recently in this laboratory for synthesis

of 1-substituted-2-thio-6-allylamino-4-[2-isobutoxy-5(4-methyl-5-carboxy-1,3-thiazo-2-yl)]-

phenyl-1,3,5-triazines (IXa-h) by isomerisation of 2-substitutedimino-6-methylamino-4-[2-

isobutoxy-5(4-methyl-5-carboxy-1,3-thiazo-2-yl)]-phenyl-1,3,5-thiadiazine (VIIIa-h) by

making use of lemon juice as a biocatalyst. This synthetic method has very efficient way to

accelerate the course of many organic reactions, producing high yields, no by products and

consequently easier work-up and purification of the products. The structures of all the

synthesized compounds were justified on the basis of chemical characteristics, elemental

analysis and spectral studies.

Keywords: 2-substitutedimino-6-penylamino-4-[2-isobutoxy-5(4-methyl-5-carboxy-1,3-thiazo-

2-yl)]-phenyl-1,3,5-thiadiazine, one pot synthesis.

Introduction

In recent years green chemistry is new branch developed chemistry which is

completely focused on eco friendly and green synthetic method1-3

. This is a recent,

developing and up growing branch of science in which numbers of attempts will be done for

conniving, budding and execution of research schemes. So now-a-days it became prime duty

of chemist to carry out selectivity in modern synthesis4

.1,3,5-Triazine nucleus have attracted

a great deal of attention among chemists due to its diverse biological activities such as

antimicrobial5

anti-protozoal6 anti-cancer

7 anti-malarial

8 antiviral

9 anti-tumer

10 anti-

inflammatory11

and anti-depresent12

. Organic fluorescent heterocyclic chromophore have a

wide range of applications in molecular probes13

fluorescent markers14

organic light-emitting

diodes (OLED)15

photovoltaic cells16

and in traditional textile and polymer fields17

. Electron

donors like triphenylamine, diphenylamine, 1,3,5-triazines and carbazoles with high electron

mobility, thermal and photochemical stability are commonly used as hole transporting



materials or light-emitting materials for balanced charge injection for above mentioned

application18

. Considering these facts we carried out green synthesis of 1-substituted-2-thio-

6-allylamino-4-[2-isobutoxy-5(4-methyl-5-carboxy-1,3-thiazo-2-yl)]-phenyl-1,3,5-triazines

(IXa-h) by making use lemon juice (Scheme-I).

(VIIIa-h)2-Substitutedimino-6-allylamino-4-[2-isobutoxy-

1,3-thiazo-2-yl)]-phenyl-1,3,5-thiadiazine

O

N

S

OH

O

CH3

CH3

CH3

N

N S

NH

N

CH2

R

O

N

S

OH

O

CH3

CH3

CH3

N

N N

NH

S

R

CH2

lemon juice

(IXa-h)

5(4-methyl-5-carboxy-

1-Substitutedamino-2-thio-6-allylamino-4-[2-isobutoxy-

5(4-methyl-5-carboxy-1,3-thiazo-2-yl)]-phenyl-1,3,5-triazines

Where: methyl, ethyl, tert-butyl, phenyl, p-chloro-phenyl, o-tolyl, m-tolyl, p-tolyl.

Results and discussion:

1-Methylamino-2-thio-6-allylamino-4-[2-isobutoxy-5-(4-methyl-5-carboxy-1,3-thiazolo)-

phenyl]-1,3,5-triazine (IXa)

In 100 ml round bottom flask a reaction mixture of 2-methylimino-6-allylamino-4-[2-

isobutoxy-5(4-methyl-5-carboxy-1,3-thiazo-2-yl)]-phenyl-1,3,5-thiadiazine (VIIIa) was stored

in lemon juice. It was tightly sealed and the reaction mixture was kept in sun light for 5

hours. Then the reaction mixture was poured on ice cubes with vigorous stirring, yellow

crystals were obtained these were washed several times with water.

Properties of (IXa):

M.F.:C22H25N5O3S2, Faint yellow crystalline solid, Yield-94%, M.P.1450C. It gave

positive test for nitrogen and sulphur elements and phenolic group. Desulphurised by alkaline

lead acetate solution indicating presence of C=S group. Formed picrate, M.P.1700C. %

Composition-Found(Calculated) C-55.60 (56.05), H-04.41 (05.40), N-13.38 (14.86), S-12.35

(13.58). FTIR (KBr) ν cm-1

-: 3310.00 (NH stretching), 2854.14(Ar-H stretching), 1598.00

(N-C-N stretching), 1504.20(-N-C=S stretching), 1210.17 (C=S stretching), 1074.43 (C-N

stretching). 1H NMR (400 MHz CDCl3 δ ppm), -COOH proton at 11.546 ppm, -NH proton

at 8.1244-7.9847 ppm, Ar-H protons at δ 7.5214-6.4018 ppm, CH proton at δ 5.4587 ppm,

NH proton at δ 4.4587 ppm and CH3 protons at δ 1.4456-1.2922 ppm. 13

C Spectrum: C=S



carbon at 185.24-179.14 ppm, Ar-C carbon at 156.46-130.91 ppm, -CH3 carbon at

40.06-38.80 ppm. Mass analysis: Fragmentation occurs during the analysis is given

O

N

S

OH

O

CH3

CH3

CH3

N

N N

NH

S

CH3

CH2

M+ 470.35

O

N

S

OH

O

CH3

CH3

CH3

N

N N

S

CH3

OH

N

S

OH

O

CH3

N

N N

S

CH3415.50360.40

OH

N

S

OH

O

CH3

NCH3275.50

From above chemical characteristics, elemental and spectral studies compound (IXa) was

assigned structure as 1-methylamino-2-thio-6-allylamino-4-[2-isobutoxy-5-(4-methyl-5-

carboxy-1,3-thiazolo)-phenyl]-1,3,5-triazine

O

N

S

OH

O

CH3

CH3

CH3

N

N N

NH

S

R

CH2

(IXa)

1-Methylamino-2-thio-6-allylamino-4-[2-isobutoxy-

5(4-methyl-5-carboxy-1,3-thiazo-2-yl)]-phenyl-1,3,5-triazines

Similarly,3-ethylimino[2-isobutoxyphenyl-5-(4-methyl-5-carboxy)-1,3-thiazolo]-2-

allyl-1,3,5-thiadiazine (VIIIb), 3-tert-butylimino-[2-isobutoxyphenyl-5-(4-methyl-5-

carboxy)-1,3-thiazolo]-2-allyl-1,3,5-thiadiazine(VIIIc)3-phenylimino[2-isobutoxyphenyl-5-

(4-methyl-5-carboxy)-1,3-thiazolo]-2-allyl-1,3,5-thiadiazine (VIIId) 3-p-cl-phenylimino[2-

isobutoxyphenyl-5-(4-methyl-5-carboxy)-1,3-thiazolo]-2-allyl-1,3,5-thiadiazine(VIIIe), 3-o-

tolylimino[2-iso butoxyphenyl-5-(4-methyl-5-carboxy)-1,3-thiazolo]-2-allyl-1,3,5-thiadiazine

(VIIIf) 3-m-tolylimino[2-isobutoxyphenyl-5-(4-methyl-5-carboxy)-1,3-thiazolo]-allyl-1,3,5-

thiadiazine (VIIIg) and 3-p-tolylimino[2-isobutoxyphenyl-5-(4-methyl-5-carboxy)-1,3-



thiazolo]-2-allyl-1,3,5-thiadiazine (VIIIh) were isomerized by lemon juice by above

mentioned method to isolate 1-ethylamino-2-thio-6-allylamino-4-[2-isobutoxy-5-(4-methyl-

5-carboxy-1,3-thiazolo)-phenyl]-1,3,5-triazine (IXb), 1-tert-butylamino-2-thio-6-allylamino-

4-[2-isobutoxy-5-(4-methyl-5-carboxy-1,3-thiazolo)-phenyl]-1,3,5-triazine (IXc) 1-

phenylamino-2-thio-6-allylamino-4-[2-isobutoxy-5-(4-methyl-5-carboxy-1,3-thiazolo)-

phenyl]-1,3,5-triazine (IXd) 1-p-chlorophenyl amino-2-thio-6-allylamino-4-[2-isobutoxy-5-

(4-methyl-5-carboxy-1,3-thiazolo)-phenyl]-1,3,5-triazine (IXe), 1-o-tolylamino-2-thio-6-

allylamino-4-[2-isobutoxy-5-(4-methyl-5-carboxy -1,3-thiazolo)-phenyl]-1,3,5-triazine (IXf)

1-m-tolylamino-2-thio-6-allylamino-4-[2-isobutoxy-5-(4-methyl-5-carboxy-1,3-thiazolo)-

phenyl]-1,3,5-triazine (IXg) and 1-p-tolylamino-2-thio-6-allylamino-4-[2-isobutoxy-5-(4-

methyl-5-carboxy-1,3-thiazolo)-phenyl]-1,3,5-triazine (IXh) respectively and enlisted in

Table No. 1.

Sr.

No.

1-allyl -2-Substituedamino[2-isobutoxy-5-(4-methyl-5-carboxy-

1,3-thiazolo)phenyl]-6-thial-1,3,5-trazines (IXa30-a37)

Yield

%

m.p.

0C

1. ----2-methylamino----------- 85 123

2. ----- -2-ethylamino---------- 83 105

3. ---- -2-tert-butylamino-------- 78 166

4. ------ -2-phenylamino--------- 76 180

5. ---- -2-p-cl-phenylamino------- 75 190

6. ------ -2-o-tolylamino------------ 76 154

7. ------ -2-m-tolylamino------------- 78 198

8. ------ -2-p-tolylamino---------------- 76 207

References:

1. Verma R.S., ―Green Chemistry , Challenging Perspective‖ Oxford University Press ,

Oxford , Pg 221, 2000.

2. Verma R.S., ―Microwaves in Organic Synthesis, Chap. 6, Wiley–VCH, Weinheim , PP

181, 2002.

3. Doble M. and Kruthiventi A.K., Green Chemistry and Engineering, Academic Press,

2007.

4. Luche J.L. and Bianchi C., Synthetic Organic Sonochemistry, Springer, US 1998.

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14. Fox M., Intro. electron transfer a critical link between sub disciplines in chemistry.

Chem. Rev., 92, 1992, 365-368.

15. Lakowicz J., Topics in fluorescence spectroscopy. Plenum Press, New York, 4, 1994, 504

16. Balaganesan B., Wen S,, Chen C., Tet. Lett.,44, 2003, 145-147.

17. Yu G., Gao J., Hummelen J., Wudl F., Heeger A., hetero junctions. Science 270, 1995,

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19. Padalkar V.S., Patil V.S. and Seker N.,chem..central J. 5, 2011, 77.

20. Rossbach V., Oberlein G., Kricheldorf H. R., Marcel D., ―Thermostable

Polyheterocyclicsin Handbook of Polymer Synthesis” New York, 19, 1992.

Solvent Free Synthesis Of O-Substitutedthiocarbamido-2-Aminobenzothiazole By

Microwave Irradiation Technique

Rahim Ullah Sa*

, K.S.Panpaliyaa

, S.P.Chaudharib, D.T.Tayade

a


b:Nanotechnology Research Laboratory, Shri Shivaji Science College, Amravati-444 603

*corresponding author - [email protected], [email protected]

Abstract

A novel and solvent free suitable method has been developed for a synthesis of o-

substitutedthiocarbamido-2-aminobenzothiazoles (IIIa-f) by an interaction of o-chloro-2-

aminobenzothiazole (Ia) with various thiourea (IIa-f) by microwave irradiation technique.

The structure determination and justification of the synthesized compounds were done on the

basis of elemental analysis, chemical characteristics and spectral studies.

Keywords:-Various thiourea, o-chloro-2-aminobenzothiazole and microwave oven.

Introduction



In the field of life sciences organic chemistry is the main stream and synthetic chemistry

is key stream of organic chemistry. Numerous organic molecules were synthesized by many

researcher and numbers of them were used in polymer, coordination, pharmaceutical,

agricultural, biochemical and industrial sciences due to their significant applications which

involves the development and synthesis of newer types of molecules1-5

. In recent decades

green synthesis created its own importance for protecting eco system and various techniques

are developed in this directions and number of researchers are working on this. Benzothiazolo

nucleus containing molecules showed various important applications in life, technical and

industrial sciences6-15

. From a literature survey it was noticed that not a single reference was

seen on the interaction of o-chloro-2-aminobenzothiazole (Ia) and various thiourea (IIa-f) by

this type of technique, hence considering all these facts this research scheme was designed to

synthesized o-thiocarbamido-2-aminobenzothiazoles (IIIa-f). The tentative reaction is

depicted below for m-thiocarbamido-2-aminobenzothiazoles (Scheme-I)

N

S

NH2

Cl

+ C

S

NHRNH2

m-Chloro-2-aminobenzothiazoleSubstituted thiourea

N

S

NH2

NHC

S

RHN

(I) (IIa-f)

(IIIa-f)m-substitutedthiocarbamido-2-aminobenzothiazole

Where, R= -H, -phenyl, -methyl, -allyl, 2-chlorophenyl. (Scheme-I)

Instruments and methods:

Melting points of all synthesized compounds were checked by using paraffin oil and

uncorrected. Carbon, hydrogen and nitrogen analysis was carried out on Carlo-Ebra-1106

analyzer and Colman-N-analyzer-29 respectively. IR spectra were recorded on SCIMADZU

FTIR spectrometer in the range 4000-400 cm-1

in KBr pellets. PMR spectra were recorded on

BRUKER AVANCE II 400 NMR spectrometer with TMS as an internal standard using

CDCl3 and DMSO-d6 as a solvent. All chemicals were used of A.R. grade except ethyl

isothiocyanate (Germany make).

Results And Discussion

Green synthesis of o-ethylthiocarbamido-2-aminobenzothiazole (IIIa)

A reaction mixture of o-chloro-2-aminobenzothiazole (I)and phenylthiourea (IIa)was

kept in microwave oven and irradiation was carried out for 2 minutes. Then the reaction

mixture was poured on ice cold water, with vigorous stirring, lemon coloured crystals were

separated out. Recrystallized from aqueous ethanol.



Properties of (IIIa): Lemon coloured crystalline solid, M.F. C10H12N4S2, Yield 96%, M.P.

1380C;Elemental analysis : Found(Calculated)C:46.70(47.61);H:04.12

(0.476); N:17.34(22.22); S:24.82(25.39)., IR(cm-1

): 3445.39str.,2044.70 Str. ,

1633.59str.,1399.57str.,1218.64str,1058.66str.;1HNMR(400MHzDMSO-d6):

9.9877 ppm thioamido NH proton flanked in between thioamido and benzothiazolo

molecules; 8.0389 ppm Ar-H protons of benzothiazole; 7.8155-6.9936 ppm Ar-H

protons; 5.4301ppm NH2 protons; 2.5883 ppm CH2 protons; 1.4196 ppm CH3 protons.,

LC-MS (m/z) Mol. Wt.252, 252(M+), 135, 161, 194.27.

Similarly, thiourea, methylthiourea, allylthiourea,phenylthiourea and p-chlorophenyl-

thiourea were interacted with o-chloro-2-aminobenzothiazole respectively by above

mentioned method to obtained o-thiocarbamido-2-aminobenzothiazole (IIIb), o-methyl-

thiocarbamido-2-aminobenzothiazole (IIIc), o-allylthiocarbamido-2-aminobenzothiazole

(IIId), o-phenylthiocarbamido-2-aminobenzothiazole (IIIe), and o-(p-chloro)phenylthiocarba-

mido-2-aminobenzothiazole (IIIf)respectivelyand are given in Table No.1.

Table No.1.

Sr.

No.

o-H/Substitutedthiocarbamido-2-aminobenzothiazole Time

(Seconds)

Yield

(%)

M.P.

(0C)

1 o-Thiocarbamido-2-aminobenzothiazole 60 91 146

2 o-Methylthiocarbamido-2-aminobenzothiazole 90 90 142

3 o-Allylthiocarbamido-2-aminobenzothiazole 120 92 129

4 o-Phenylthiocarbamido-2-aminobenzothiazole 120 96 154

5 o-(4-Chloro)phenylthiocarbamido-2-aminobenzothiazole 120 88 162

Conclusion:

This technique has broad applications due to its efficiency for accelerating a course of

organic reactions and increases yields, higher selectivity. This technique has consequently

easier work-up and products obtained are in comparatively pure form. In this technique

involves solvent free synthesis and green parameters are also maintained.

Acknowledgement:

Authors are thankful to Mr. Avatar Sing, Mr. Manish Kumar and authorities of S.A.I.F.

Punjab University, Chandigarh for spectral and elemental analysis.

References:



1. Middleton E., Kandaswami C., Theoharides T. C., The effects of plant flavonoids on

mammalian cells: Implications for inflammation, heart disease, and cancer.

Pharmacol. Rev. 2000; 52: 673-751.

2. Chaudhary P., Sharma, P. K., Sharma A., Varshney J., ―Recent advances in

pharmacological activity of benzothiazole derivatives‖; International journal of

current pharmaceutical research, 2010, 2, 5

3. Musser J. H., Brown R. E., Love B., Baily K., Jones H., Kahen R., Synthesis of 2-

(2,3-Dihydro-2-oxo-l,3,4-oxadiazol-5-yl) Benzo Heterocycles. A Novel Series of

Orally Active AntiallergicAgents.; J Med Chem. 1984, 27, 121.

4. Yoshida M., Hayakawa I., Hyashi N., Agatsuma T., Oda Y., Tanzawa F. Synthesis

and biological evaluation of benzothiazole derivatives as potent antitumor

agents,Bioorg Med Chem. Lett., 2005, 15, 3328.

5. Gupta S., Ajmera N., Gautam N., Sharma R.,Gauatam D., Novel synthesis and

biological activity study of pyrimido [2,1-b] benzothiazoles. Ind J Chem. 2009,

48B:853-858.

6. Kumbhare R.M., Ingle V.N., Synthesis of novel benzothiozole and, benzisoxazole

functionalized unsymmetrical alkanes and study of their antimicrobial activity. Ind J

Chem. 2009; 48 996-1000.

7. Murthi Y., Pathak D., Synthesis and Antimicrobial screening of substituted 2-

Mercaptobenzothiazoles. J Pharm Res. 2008; 7(3); 153-155.

8. Rajeeva B., Srinivasulu N., Shantakumar S., Synthesis and Antimicrobial activity of

some new 2-substituted benzothiazole derivatives. E-Journal of Chemistry 2009,

6(3):775-779.

9. Maharan M., William S.,Ramzy F., Sembel A., Synthesis and in vitro Evaluation of

new benzothiazolederivaties as schistosomicidal agents. Molecules 2007; 12: 622

10. Kini S., Swain S., Gandhi A., Synthesis and Evaluation of novel Benzothiazole

Derivates against Human Cervical Cancer cell lines. Ind J Pharm Sci. 2007;

11. Stanton H.L.K., Gambari R., Chung H.C., Johny C.O.T., Filly C., Albert S.C.C.,

Synthesis and anti-cancer activity of benzothiazole containing phthalimide on human

carcinoma cell lines. Bioorg Med Chem. 2008; 16:3626-3631.

12. Wang M., Gao M., Mock B., Miller K., Sledge G., Hutchins G.,Zheng Q., Synthesis

of C-11 labelled fluorinated 2-arylbenzothiazoles as novel potential PET cancer

imaging agent. Bioorg Med Chem. 2006; (14):8599-8607.



13. Gupta S, Moorthi N, Sanyal U. Synthesis, cytotoxic evaluation, in silico

pharmacokinetic and QSAR study of some benzothiazole derivatives. Ind J Pharmacy

Pharm Sci. 2010; 2(3):57-62.

14. Sreenivasa M.,jaychand E., Shivakumar B., Jayrajkumar K.,Vijaykumar J., Synthesis

of bioactive molecule flurobenzothiazole comprising potent heterocylic moieties for

anthelmintic activity. Arch Pharm Sci and Res. 2009; 1(2):150-157.

15. Reddy P, Lin Y, Chang H. Synthesis of novel benzothiazole compounds with an

extended conjugated system. Arcivoc. 2007, 113-122.

A Novel Green Synthesis Of 1-Phenyl-3-[4-(2,4-Dithio-3-T-Butyl-5 Substituted-1,3,5-

Triazino) Aminophenyl]-Prop-2-Ene-1-Ones

S.S. Padhen1

, D. T. Tayade2*

, D.A.Pund3

1 Department of Chemistry, RajarsheeShahu Science College, Chandur( Rly) Dist Amravati 2*

Department if Chemistry, Govt. Vidarbha Institute of Science & Humanities, Amravati- 3

Department of Chemistry, Jawarharlal Darda Institute of Engineering and Technology

Yavtmal

*Corresponding author Email- [email protected], [email protected]

Abstract:

Recently in this laboratory a potent series of 1-phenyl-3-[4-(2,4-dithio-3-t-butyl-5-

substituted-1,3,5-triazino)aminophenyl]-prop-2-ene-1-ones (IIa-e) was synthesized by the

isomerization of 1-phenyl-3-[4-(2-t-butylimino-4-substitutedimino-1,3,5-dithiazino)amino-

phenyl] prop-2-ene-1-ones (Ia-e)in 10% aqueous ethanolic sodium bicarbonate medium by

maintain green chemistry parameters. Synthesized compounds are recrystallized from ethanol

and their structure was justified on the basis of chemical characteristics, elemental analysis

and spectral analysis.

Introduction:

1,3,5-Triazine compounds have wide range of applications in different fields,

including the production of herbicides and polymer photostabilisers1.The diverse biological

activities observed for different molecules containing the 1,3,5-triazine unit have been further

explored in order to discover new potential molecules through the synthesis of libraries by

combinatorial approaches2. In 1,3,5-triazines molecules have the positions 2, 4 or 6 stronger

bond is generated which causes more restriction to free rotationdue to it containing electron

donating groups, such as the amino group3. Hence 1,3,5-Triazine nucleus have attracted a

great attention among researchers due to its diverse biological activities such as

antimicrobial4-5

anti-protozoal6 anti-cancer

7 anti-malarial

8 antiviral

9 anti-tumer

10anti-

inflammatory11

and anti-depresent12

.Therefore, it is quite interesting to investigate the

mailto:[email protected]



isomerization of 1-phenyl-3-[4-(2-t-butylimino-4-substitutedimino-1,3,5-dithiazino)amino-

phenyl]prop-2-ene-1-ones (Ia-e) on isomerizing by refluxing with 10% aqueous sodium

bicarbonate solution in ethanol to isolate 1-phenyl-3-[4-(2,4-dithio-3-t-butyl-5-substituted-

1,3,5-triazino)aminophenyl]-prop-2-ene-1-ones (IIa-e) hence this work was carried out.

Materials and Method -

All the chemical used in the present research were MERCKS (India Made). Starting

compounds (Ia-e) were synthesized by literature method13

.

Method

Method adopted for the synthesis of all the compounds in the present investigation

was conventional refluxing under water bath to attain constant temperature. Melting points of

all the synthesized compounds estimated using paraffin oil and uncorrected. The carbon,

hydrogen and nitrogen analysis was carried out on Carlo-Ebra-1106 analyzer and Colman-N-

analyzer-29 respectively. IR spectra were recorded on SCIMADZU FTIR spectrometer in the

range 4000-400 cm-1

in KBr pellets. PMR spectra were recorded on BRUKER AVANCE II

400 NMR spectrometer with TMS as an internal standard using CDCl3 and DMSO-d6 as a

solvent.

Experimental

General Procedure

1-Phenyl-3-[4-(2-t-butylimino-4-substitutedimino-1,3,5-dithiazino)aminophenyl] prop

-2-ene-1-ones (Ia-e) was isomerized by 10% aqeous sodium bicarbonate solution. Reactant

dissolved into the solvent during heating. After distillation of excess solvent yellow crystals

were obtained, which recrystallized from glacial acetic acid to isolate 1-phenyl-3-[4-(2,4-

dithio-3-t-butyl-5-substituted-1,3,5-triazino)aminophenyl]-prop-2-ene-1-ones (IIa-e) hence

this work was carried out.

The tentative reaction is given below,



O

NH

S

N

S

N

NR

1

CH3

CH3

CH3

O

NH

N

N

N

S

SR

1

CH3

CH3

CH3

1-phenyl-3-[4-(2,4-dithio-3-t-butyl-5-substituted-1,3,5-triazino) aminophenyl]-prop-2-ene-1-ones

(IIa-e)

(Ia-e)

Where R1= allyl, ethyl, t-butyl, phenyl, p-Cl-phenyl

1-phenyl-3-[4-(2-t-butylimino-4-substitutedimino-1,3,5-dithiazino)amino phenyl ] prop-2-ene-1-ones

NaHCO3 / EtOH Isomerisation

Similarly, 1-phenyl-3-[4-(2-t-butylimino-4-allylimino-1,3,5-dithiazino)aminophenyl]

prop-2-ene-1-one (Ia), 1-phenyl-3-[4-(2-t-butylimino-4-ethylimino-1,3,5-dithiazino)-amino

phenyl]-prop-2-ene-1-one (Ib), 1-phenyl-3-[4-(2-t-butylimino-4-t-butylimino-1,3,5-

dithiazino)-aminophenyl]-prop-2-ene-1-one (Ic), 1-phenyl-3-[4-(2-t-butylimino-4-phenyl

imino-1,3,5-dithiazino)-aminophenyl]-prop-2-ene-1-one (Id) and 1-phenyl-3-[4-(2-t-butyl

imino-4-p-Cl-phenylimino-1,3,5-dithiazino)-aminophenyl]-prop-2-ene-1-one (Ie) were

interacted with 10% sodium bicarbonate in ethanol by above mentioned method to obtained1-

phenyl-3-[4-(2,4-dithio-3-t-butyl-5-allyl-1,3,5-triazino) aminophenyl]-prop-2-ene-1-one(IIa),

1-phenyl-3-[4-(2,4-dithio-3-t-butyl-5-ethyl-1,3,5-triazino)aminophenyl]-prop-2-ene-1-one

(IIb), 1-phenyl-3-[4-(2,4-dithio-3-t-butyl-5-t-butyl-1,3,5-triazino)aminophenyl]-prop-2-ene-

1-one(IIc),1-pheny l-3-[4-(2,4-dithio-3-t-butyl-5-phenyl-1,3,5-triazino)aminophenyl]-prop-2-

ene-1-one(IId), 1-phenyl-3-[4-(2,4-dithio-3-t-butyl-5-p-Cl-phenyl -1,3,5-triazino) amino

phenyl]-prop-2-ene-1-one (IIe).

Result and Discussion:

1-Phenyl-3-[4-(2,4-dithio-3-t-butyl-5-allyl-1,3,5-triazino)aminophenyl]-prop-2-ene-1-one

(IIa)

Yellow solid, C25H26N4OS2, Yield-72%, M.P.-1890C Composition-found(calculated) C-

63.89(64.90), H-6.65(5.66), N-11.11(12.11) and S-12.85(13.86); FTIR(KBr)νcm-1

:

3066.15(ArC-H stretching), 3355.16(N-H stretching),1679.49(C=O stretching), 1217.89(C-N

stretching) and 1139.64 (C=S stretching); 1H NMR (400 MHz CDCl3 δ ppm): doublet of

2H, -CH=CH- at δ 3.66-3.88ppm,multiplet of 9H of Ph at δ 6.67-8.14ppm, singlet of 1H of –

NH at δ 9.80ppm,singlet of 9H, CH3 at δ 1.37 ppm, quintet of 1H and double doublet of 2H

of allyl at δ 2.22, 1.31 and 2.10 respectively; Mol. Wt.: 522.



1-Phenyl-3-[4-(2,4-dithio-3-t-butyl-5-ethyl-1,3,5-triazino)aminophenyl]-prop-2-ene-1-

one (IIb)

Lemon yellow solid, C24H26N4OS2, Yield-78%, M.P.-1960C Composition-

found(calculated) C-62.96(63.97), H-6.82(5.82), N-11.41(12.43) and S-13.21(14.23); FTIR

(KBr) ν cm-1

:3046.95(ArC-H stretching), 3347.74(N-H stretching), 1672.86(C=O

stretching), 1209.13 (C-N stretching) and 1131.35 (C=S stretching); 1H NMR (400 MHz

CDCl3 δ ppm)doublet of 2H of –CH=CH- at δ 3.57-3.78ppm, multiplet of 9H of Ph at δ

6.55-8.01ppm, singlet of 1H of –NH at δ 8.19ppm, singlet of 9H, CH3 at δ 1.22ppm, quartet

of 2H and triplet of 3H of ethyl at δ1.33 and δ 1.37 respectively; Mol. Wt.: 450.

1-Phenyl-3-[4-(2,4-dithio-3-t-butyl-5-t-butyl-1,3,5-triazino)aminophenyl]-prop-2-ene-1-

one (IIc)

Yellow solid, C26H30N4OS2, Yield-85%, M.P.-1840C Composition-found(calculated)

C-64.23(65.24), H-7.35(6.32), N-10.69(11.70) and S-12.28(13.40); FTIR (KBr) ν cm-

1:3045.32(ArC-H stretching), 3348.41(N-H stretching), 1649.60(C=O stretching),

1211.21(C-N stretching) and 1122.78 (C=S stretching); 1H NMR (400 MHz CDCl3 δ ppm)

doublet of 2H of –CH=CH- at δ 3.41-3.67ppm, multiplet of 9H of Ph at δ 6.64-

8.07ppm,singlet of 1H of –NH at δ 8.31ppm,singlet of 18H of CH3 at δ 2.53ppm; Mol.

Wt.:478.

1-Pheny l-3-[4-(2,4-dithio-3-t-butyl-5-phenyl-1,3,5-triazino)aminophenyl]-prop-2-ene-1-

one (IId)

Yellow solid, C28H26N4O3S2, Yield-79%, M.P.-1890C Composition-found(calculated) C-

66.40(67.44), H-6.23(5.26), N-10.23(11.24) and S-11.89(12.86); FTIR (KBr) ν cm-

1:3039.58(ArC-H stretching), 3339.67(N-H stretching), 1641.87(C=O stretching), 1214.88

(C-N stretching) and 1128.81 (C=S stretching); 1H NMR (400 MHz CDCl3 δ ppm)doublet

of 2H of –CH=CH- at δ 3.47-3.78ppm, multiplet of 14H of Ph at δ 6.62-8.06ppm,singlet of

1H of –NH at δ 8.43ppm,singlet of 9H, CH3 at δ 1.34ppm; Mol. Wt.: 498.

1-Phenyl-3-[4-(2,4-dithio-3-t-butyl-5-p-Cl-phenyl -1,3,5-triazino)aminophenyl]-prop-2-

ene-1-one (IIe)

Pale yellow solid, C28H25N4OS2Cl, Yield-73%, M.P.- 1980C Composition-

found(calculated) C-62.07(63.08), H-5.7 2(4.73), N-9.55(10.51), S-11.01(12.03) and Cl-

7.60(6.65); FTIR (KBr) ν cm-1

:3049.43(ArC-H stretching), 3346.11(N-H stretching),

1647.28(C=O stretching), 1224.39 (C-N stretching) and 1137.82 (C=S stretching); 1H NMR

(400 MHz CDCl3 δ ppm) doublet of 2H of –CH=CH- at δ 3.35-3.57ppm, multiplet of 13H



of Ph at δ 6.78-8.04ppm, singlet of 9H, CH3 at δ 1.17ppm and singlet of 1H of –NH at δ

8.52ppm; Mol. Wt.: 505.5.

Conclusion:

The novel of this work is that triazines which have important biological and medicinal

importance can be synthesized in one step process having higher yield at the same time the

purity of the products were maintained. The green chemistry parameter in these synthesized

were maintain as we having using aqueous ethanol and very few amount of sodium

bicarbonate for the synthesis no other reactants were used.

Acknowledgement:

Authors are thankful to Mr. Avatar Sing, Mr. Manish Kumar and authorities of

S.A.I.F. Punjab University, Chandigarh for spectral and elemental analysis

REFERENCES

1. Hollink E., Simanek E. E., Bergbreiter D. E., Tetrahedron Lett. 46 2005, 2005-2008.

2. Khersonsky S. M., Jung D.W., Kang T.W., Walsh D. P., Moon H.S., Jacobson E.M.,

Shetty V., Neubert T. A., Chang Y.T., J. Am. Chem. Soc. 2003,125, 11804-11805.

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Migallón, Valiente G., Org. Biomol. Chem. 2003, 4451-4457 .

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R.K., Biorg. Med. Chem. Lett.18, 2008, 1308.

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Eur. J. Med. Chem. 41, 2006, 1240.

6. Alessandro B., Gorka J.B., Mhairi L.S., Vanessa Y.,Reto B., Michael P.B., Ian H.G., J.

Med. Chem. 48, 2005, 5570.

7. Rita M., Simona S., Giovanni S., Francesca V., LisaD.V., J. Med. Chem. 2004, 4649.

8. Sergio, M.; Davide, P.; Paolo, C.; Nicoletta, B.; Diego, M. J, Med. Chem., 3, 2008,

9. Yuan-Zhen X., Fen-er C., Jan B., Erik D.C., ChristopheP.,Eur. J. Med. Chem., 43,

2008, 1230.

10. Krutz L.J., ShanerD.L.,Weaver M.A., Webb R.M.T Zablotowicz R.M., Reddy K.N.

Huang Y. and Thomson S.J., Pest Management Sci., 66(5), 2010, 461-481.

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12. Zhuo J., He C., Yao W., United States, Patent Application Publication,

US2013/0345224 A1, 2013.



13. Tayade D.T., ―A Contribution to the chemistry of nitrogen, nitrogen and sulphur

containing heteroacyclic and heterocyclic compounds‖, Ph.D. Thesis, Amravati

University, Amravati, 1996.

Green Synthesis Of 8-[(2-Thio-3-Methyl-6-Substitutedamino)-1,3,5-Thiadiazino]Imino-

1-Methyl-6-Phenyl-4h-[1,2,4] Triazolo [4,3-A] [1,4] Benzodiazepines

P. R. Kalea, D.T.Tayade

a*, G. D. Tayade

b

aDepartment of Chemistry, Government Vidarbha Institute of Science and Humanities, Amravati bDepartment of Physics, Government Vidarbha Institute of Science and Humanities, Amravati

*corresponding author [email protected]

Abstract

Recently a novel series of 8-[(2-thio-3-methyl-6-substitutedamino)-1,3,5-

thiadiazino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo [4,3-a] [1,4] benzodiazepines was

successfully synthesized by the isomerisation of 8-[(2-methylimino-6-substitutedamino)-

1,3,5-dithiozino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo [4,3-a] [1,4] benzodiazepines

by 5% aqueous sodium bicarbonate in ethanol medium. Structures of all synthesized

compounds were justified on the basis of chemical characteristics, elemental analysis and

spectral studies.

Keywords:-8-[(2-Methylimino-6-substitutedamino)-1,3,5-dithiozino]imino-1-methyl-6-

phenyl-4H-[1,2,4]triazolo [4,3-a] [1,4] benzodiazepines and 5% aqueous sodium bicarbonate

in ethanol.

Introduction:

Benzodiazepine based heterocycles possess biological, medicinal and industrial

applications1-5

. Its analogs are also used as dyes for acrylic fibers6. In our laboratory synthetic

applications of –thiocarbamido, -amino, -halo, -cyano, were successfully explored and their

biological and physical applications were also studied7-10

. Hence this research scheme was

designed to synthesize a novel series of8-[(2-thio-3-methyl-6-substitutedamino)-1,3,5-

thiadiazino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a] [1,4] benzodiazepine from an

isomerisation of 8-[(2-methylimino-6-substitutedamino)-1,3,5-dithiazino]imino-1-methyl-6-

phenyl-4H-[1,2,4]triazolo[4,3a] [1,4]benzodiazepines in5% aqueous sodium bicarbonate in

ethanol medium,

Synthesis of 8-[(2-thio-3-methyl-6-phenylamino)-1,3,5-thiadiazino]imino-1-methyl-6-

phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine



Synthesis of 8-[(2-thio-3-methyl-6-phenylamino)-1,3,5-thiadiazino]imino-1-methyl-6-

phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine was carried out by isomerizing 8-[(2-

methylimino-6-phenylamino)-1,3,5-dithiozino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo

[4,3-a] [1,4] benzodiazepine in 5% aqueous sodium bicarbonate solution in ethanol. After

distillation of excess solvent yellow crystals were separated out. Recrystalised from glacial

acetic acid.Yield 90%, m.p. 210˚C.

Properties of Product:

It is brown colour crystalline solid having melting point 2100C. It gave positive test

for nitrogen and sulphur. It was desulphurized by alkaline plumbite solution which clearly

indicate the presence of C=S group. It was soluble in water, ethanol, DMSO-d6 while

insoluble in carbon tetrachloride, chloroform, benzene, petroleum ether. It formed picrate

having melting point 1800C. Elemental analysis: Gave satisfactory elemental analysis., IR

Spectrum: The IR spectrum was carried out in KBr-pellets The important absorptions are

correlated as (cm-1

) 3379.29 N–H stretching, 2887.44 C-H stretching, 1658.78 N=C-N

stretching, 1288.45 N-C=S stretching, 1083.99 C-N stretching, 731.02 monosubstituted

benzene., NMR Spectrum: The NMR spectrum was carried out in DMSO-d6 and CDCl3

This spectrum distinctly displayed the signals due to Ar-H protons at 8.2234-7.0495 ppm, -

NH proton at 3.3542-3.3302 ppm, -CH2 protons at 2.5333-2.5156 ppm, -CH3 protons at

1.3152 ppm. Similarly, 8-[(2-methylimino-6-ethylamino)-1,3,5-dithiozino]imino-1-methyl-6-

phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine,8-[(2-methylimino-6-methylamino)-

1,3,5 -dithiozino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3a][1,4]benzodiazepine,8-

[(2-methylimino-6-tert-butylamino)-1,3,5-dithiozino]imino-1-methyl-6-phenyl-4H-[1,2,4]

triazolo[4,3-a][1,4]benzodiazepine,8-[(2-methylimino-6-p-chlorophenylamino)1,3,5-

dithiozino] imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3a][1,4]benzodiazepine, 8-[(2-

methylimino-6-p-tolylamino)-1,3,5-dithiozino]imino-1-methyl-6-phenyl-4H

[1,2,4]triazolo[4,3-a][1,4] benzodiazepine were isomerized by 5% aqueous sodium

bicarbonate solution by above mentioned method to isolate 8-[(2-thio-3-phenyl-6-

ethylamino)-1,3,5-thiodiazino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-

a][1,4]benzodiazepine,8-[(2-thio-3-methyl-6-methylamino)-1,3,5-thiodiazino]imino-1-

methyl-6-phenyl-4H-[1,2,4]-triazo lo[4,3-a] [1,4] benzodiazepine, 8-[(2-thio-3-methyl-6-

tert-butylamino)-1,3,5-thiodiazino]- imino-1-methyl-6-phenyl-4H-[1,2,4] triazolo[4,3-a] [1,4]

benzodiazepine, 8-[(2-thio-3-methyl-6-p-chlorophenyl amino)-1,3,5-thiodizino]imino-1-

methyl-6-phenyl-4H-[1,2,4] triazolo[4,3-a] [1,4] benzodi azepine, 8-[(2-thio-3-methyl-6-o-



tolylamino)-1,3,5-thiodizino] imino-1-methyl-6-phenyl-4H-[1,2,4] triazolo [4,3-

a][1,4]benzodiazepine by above mentioned method and enlisted in Table No. I

Table No. I

Sr.

No.

8-[(2-Thio-3-methyl-6-substitutedamino)-1,3,5-

thiadiazino]imino-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-

a][1,4] benzodiazepine

Yield

(%)

m.p.

(0C)

1 8-[(2-Thio-3-methyl-6-ethylamino)--------benzodiazepine 92 206

2 8-[(2-Thio-3-methyl-6-methylamino)-------benzodiazepine 94 240

3 8-[(2-Thio-3-methyl-6-t-butylamino)-------benzodiazepine 90 198

4 8-[(2-Thio-3-methyl-6-p-chlorphenylamino)----benzodiazepine 88 245

5 8-[(2-Thio-3-methyl-6-p-tolyl-amino)--------- benzodiazepine 86 260

References:

1. Hulme C., Peng J., Tang S. Y., Burns C. J., Morize I., Labaudiniere R. J., Org. Chem. 1998, 63,

2. Keating T. A., Armstrong R. W., J. Org. Chem. 1996, 61, 8935.

3. Kalinski C., Umkehrer M., Ross G., Kolb J., Burdacka, C., Hiller W., Tetrahedron Lett. 2006, 47,

4. Aversa M. C., Ferazzo A., Giannetto P., Kohnke F. H., Synthesis1986, 230.

5. Chimirri A., Grasso S., Ottana R., Romeo G., Zappala M., J. Heterocyclic Chem. 1990, 27, 371.

6. Herbert J. A. L., Suschitzky H. J. Chem. Soc., Perkin Trans. 1 1974, 2657.

7. Bansal R.K., J.Heterocycyclic Chemistry, 8, 2012, 12-24.

8. Fernandes P.S and Sonar T.M., J.Ind.Chem.Soc., 53(4),1986, 427.

9. Saleem F., Eur. Pat., CHAPPL 87/1 APR 13, 3600009 (1987), Chem Abstr.110,1989, 114893.

10. Hedge J.C.,SatheeshaRai N. and Balkrishna K., J.Chem.Sci.,III 9(4),2007, 299-302.

Green Synthesis Of 2-[-2-Hydroxy-5-(2,4-Substituted-Dithiobiureto)] Phenylindoles

R. D. Isankara, D. T. Tayade

a*, G. G. Mule

b


444 604, MS bDepartment of Physics, S.G.B. Amravati University, Amravati 444 604, MS

* Corresponding Author [email protected], [email protected]

Abstract

In this work we synthesized a novel series of 2-[-2-hydroxy-5-(2,4-substituted-

dithiobiureto)]phenylindole by green synthesis parameters from 2-(-2-hydroxy-5-substituted-

thiocarbamidophenyl)indoles and substituted isothiocynate. Previously such types of



reactions were carried out in acetone medium but we developed new reaction condition in

which percentage of acetone was reduced. During synthesis we carried out these reactions in

various compositions of ethanol-acetone medium. This method has broad applications due to

its efficiency for accelerating a course of organic reactions and increases yields, higher

selectivity and lower quantities of byproducts. This method has consequently easier work-up

and products obtained are in comparatively pure form. In 70% ethanol-acetone medium we

got maximum yields. Justification and confirmations of structure of synthesized compounds

were carried by usual elemental analysis, chemical characteristics and spectral studies.

Keywords: 2-[-2-Hydroxy-5-(2,4-substituteddithiobiureto)] phenylindoles, isothiocynate.

Introduction

Indole based molecules are reported for their biological and medicinal significances

having great applications1-6

. Literature survey also reveals that 2,4-dithiobiureto nucleus

containing heteroacycles and heterocycles are known for their important potent biological

activities7-11

as well as these can be easily used as a good intermediate12-15

for synthesis of

abundant significantly active numerous five, six and seven member heterocycles. It was

observed that sulphur and nitrogen of 2,4-dithiobiuret are responsible for changing biological

applications. Literature survey showed that interactions of 2-(-2-hydroxy-5-substituted-

thiocarbamido)phenylindoles and substituted isothiocynate are still lacking, hence we carried

out these interactions in ethanol-acetone medium to obtained 2-[-2-hydroxy-5-(2,4-

substituted-dithiobiureto)]phenylindoles by somewhat suitable and eco-friendly green

synthetic method.

Result and Discussion

Synthesis of 2-[-2-hydroxyl-5-(-2,4-ethyldithiobiureto)]phenylindole(IIIa)

Interaction of 2-(-2-hydroxy-5-thiocarbamido)phenylindole(I) and ethylisothiocynate

(IIa) was carried out in 70% ethanol-acetone medium for 2 hours on water bath. After

distillation of excess solvent; pale yellow crystals of product were separated out.

Recrystallized from aqueous ethanol. Yield- 92%, m.p. 2020C . During developing reaction

conditions it was observed that 70% ethanol-acetone mixture is the best solvent which

increase yield of product as well as curtail time span of reaction by maintaining the purity of

product. Results obtained during work is depicted in Table No. 1.

Table No. 1

Sr. No. Solvents Time span (Hrs) Yield (%)

1. Acetone* 16 47



2. Ethanol* 06 58

3. Benzene 08 28

4. Carban tetrachloride 12 32

5. Ethanol-Acetone(40%) 03 60





10 Ethanol-Acetone(70%) 03 61

* Literature solvents

The probable reaction for the formation of (IIIa) is depicted below,

NH

NH

OH

S

NH2

CHNH S

H5C2

ethylisothiocyanate

NH

NH

OH

NH

S S

NH CH3

(IIa)

(I)

70% ethanol-acetone

(IIIa)

+

Properties of (IIIa): Molecular formula: C18H18N4O1S2, yellow crystalline solid, m.p. 2020C.

Rf value was found to be 0.35, by using dioxane solvent on silica gel-G having layer

thickness 0.3 mm. Gave Lassigne‘s positive test for nitrogen and sulpur. It was desulphurised

by alkaline plumbite solution indicating that sulphur is present in open chain. It gave green

colour when neutral ferric chloride solution indicating that phenolic group is present. It gave

picrate having m.p. 1780. Elemental analysis: Found (Calculated) C: 60.75(61.01),

H:4.02(4.08), N:15.80(15.81), S:17.53(18.01) IR Spectrum [(KBr pellets) cm-1

]: 3450.00

(O-H stretching); 3350.00 (N-H stretching); 2972.31 (C-H stretching); 1734.01(N=C-N

stretching); 1616.36 (C=C stretching); 1541.12 (N-C=S stretching); 1149.57 (C-N

stretching)., 1H NMR (400 MHz CDCl3 δ ppm) indole -NH proton at 12.8583 ppm,

flanked thioamido -NH protons at δ 9.4863 ppm, Ar-H protons at 7.4358-6.8346 ppm, Ar-

OH proton at δ 5.6753 ppm, terminal -NH proton at δ 3.4988 ppm,-CH2 protons at 2.5367-

2.3605 ppm, -CH3 protons at 0.9651-0.8489 ppm.

Similarly,2-[(-2-hydroxy-(2,4-methyldithiobiureto)]phenylindole,2-[-2-hydroxy-(2,4–t-

butyldithiobiureto)]phenylindole, 2-[-2-hydroxy-(2,4-phenyldithiobiureto)]phenylindole and



2-[-2-hydroxy-(2,4-p-Cl-phenyldithiobiureto)]phenylindole were synthesized by interactions

of 2-(-2-hydroxy-5-thiocarbamido)phenylindole(I) and methylisothiocynate (IIb), t-butyliso-

thiocynate (IIc), phenylisothiocynate (IId) and p-Cl-phenylisothiocynate (IIe) above

mentioned method and given in TableNo.2

TableNo.2

Sr.

No. 2-[-2-Hydroxy-5-(2,4-substituteddithiobiureto)]phenylindoles

Yield

%

M.P.

0C

1 2-[(-2-Hydroxy-(2,4-methyldithiobiureto)]phenylindole 94 186

2 2-[-2-Hydroxy-(2,4-t-butyldithiobiureto)]phenylindole 92 202

3 2-[-2-Hydroxy-(2,4-phenyldithiobiureto)]phenylindole 86 178

4 2-[-2-Hydroxy-(2,4-p-Cl-phenyldithiobiureto)]phenylindole 83 187

References:

1. Kerzarea D. R. and Khedekar P. B., Indian J Pharm Sci Bioscientific Res., 6(1) 2016, 144-

2. Dhani R, Avinash A., Salenaagina S. K., Saicharan M. V., Masthanaiah T., Rathnam P. R.

and Chandana S. V., J. Chem. Pharm. Res., 3(5), 2011, 519-523.

3. Gillman P. K., Bartlett J. R., Bridges P. K., Hunt A., Patel A. J., Kantamaneni B. D. and

Curzon G., J. Neurochem., 1981, 37, 410–417.

4. Naoki M., Akihito T., Makiko N. Chie F. S, Chikawa T.I., Isomura T. and Nomura K.

Biotechnol. Biochem., 74 (9), 2010,1794–1801.

5. Abdulsalam A., Bouhfid R. and El Mokhtar E., ARKIVOC , (xii) , 2009, 337-346 .

6. Synthesis of Indole and Oxindole Derivatives Incorporating Pyrrolidino, Pyrrolo or

Imidazolo moieties, Stanley Rehn Stockholm, 2004.

7. Tayade D.T., Waghmare S.A. , American journal of pharmtech research, 6(3), 2016, 605

8. D.T. Tayade , F.Z. Mohammad, Ind. J of Pharma- ceutical sci and research, 2248-9126,

4(4), 20141-3,.

9. Tayade D.T., Thombare R.D. ,European journal of pharmaceutical and medical research ,

3(3), 2016, 443-446.

10. Lunge M.S., Shaikh R.S., Tayade D.T., International Journal of Advanced Research, 4(1),

2016, 908-912.

11. Tayade D.T.,Padhen S.S., European journal of pharmaceutical and medical research, 3(8),

2016, 538-540.

12. Tayade D.T.,Kale P.R., International Journal of AppliedResearch, 3(3), 2016, 370-374.



13. Tayade D.T.,Ingole S.P., European journal of pharmaceutical and medical research,

3(7),2016.

14. Tayade D.T., Raghuwanshi M.R., Bhagwatkar A.K., Aswale S.R., Canadian Int. J.

chemistry, 3(2), 2011, 74-78.

15. Sing A.K., Mishra G. and Joyti K., J. App. Pharm. Sci., 01(5), 2011, 44-49. Ekhallas N.,

Journal of American Sciences, 6(8), 2010, 54-57.

One Pot Synthesis Of N’-(2-Hydroxyphenyl)-N”-Substitutedthioureas

S. O. Mohoda, D.T.Tayade

a*, G.G.Jadhav

a.


444 604. b S.R. R. Lahoti Science College Morshi 444905.

* Corresponding Author- [email protected]

Abstract

In organic synthesis generally toxic and expensive catalysts or volatile and hazardous

solvents are used as medium for synthesis, so various components surrounding environment

get disturb due to pollution and eco system get disturb. Development of non-hazardous

synthetic methodology is one of the greatest challenges in synthetic chemistry. So such

medium are replaced in green synthesis either by sona-technique, microwave technique or by

making use of nonhazardous natural solvents. Hence we developed a new route for the

synthesis of N‘-(2-hydroxyphenyl)-N‖-substituted thioureas by an interactions of 2-

aminophenol and various isothiocynates by using eco-friendly solvent lemon juice in presence

of sunlight by green synthetic non-conventional method. Determination and justification of

structure of products was established by usual chemical characteristics, elemental analysis

and spectral studies.

Key Words: N‘-(2-Hydroxyphenyl)-N‖-substituted thioureas, one pot synthesis.

Introduction

In recent years life sciences and chemical research are completely focused on eco-

friendly and green synthesis for maintaining ecological system and new branch in chemical

science is developed and it is known as green chemistry1-4

. This is a recent, developing and

up growing branch of science in which numbers of attempts will be done for conniving,

budding and execution of research schemes. So now-a-days it became prime duty of chemist

to carry out selectivity in modern synthesis5. In recent decades synthetic research is mainly

focused on a development of green and eco-friendly approaches, in which continuous use of

alternative reaction conditions6-7

are developed, this technique endorse variety of solvent-free



and eco-friendly chemical reactions8-12

of low cost, facile, safe and reproducible experimental

procedures13

. Literature survey reveals that thiourea based organic molecules showed

biological and medicinal importance14-19

. Considering these facts we carried out green

synthesis ofN‘-(2-hydroxyphenyl)-N‖-substituted thioureas by making use of natural occurring

lemon juice in presence of sun light (Scheme-I).

OH

NH2

+ S C N R

OH

NH NH

S

R

Methylisothiocyanate(I)

(IIa-f)(IIIa-f)

sunlight

lemon juice

Where R= -phenyl, p-chlorophenyl, p-tolyl. (Scheme-I)

Result And Discussion

Synthesis of N’-(2-Hydroxyphenyl)-N”-methylthiourea(IIIa)

In 100 ml round bottom flask a reaction mixture of 2-aminophenol(I) and

methylisothiocynate(IIa)and lemon juice was taken. It was tightly sealed and the reaction

mixture was kept in sun light for 5 hours. Then the reaction mixture was poured on ice cubes

with vigorous stirring, yellow crystals were obtained these were washed several times with

water.

Probable reaction and mechanism of this reaction is as given as below,

Reaction

OH

NH2

+ S C N CH3

OH

NH NH

S

CH3

2-AminophenolMethylisothiocyanate 2-Methylthiocarbamidophenol(I)

(IIb)(IIIb)

acetone



Mechanism

OH

NH2

+ S C N CH3

OH

N+

N

S

CH3

H H

Methylthiocarbamidophenol

OH

NH NH

S

CH3

(IIIb)

Properties of (IIIa):

M.F.:C8H10N2OS, Faint yellow crystalline solid,Yield-92%, M.P.1540C. Gave

positive test for nitrogen and sulphur elements and phenolic group.Desulphurised by alkaline

lead acetate solution indicating presence of C=S group.Formed picrate, M.P.1370C.%

Composition-Found(Calculated) C-51.60 (52.74), H-04.50 (05.49), N-15.38 (15.38), S-16.35

(17.28). FTIR (KBr) ν cm-1

-: 3214.00 (NH stretching), 3037.01(OH stretching),

2654.14(Ar-H stretching), 1598.00(N-C-N stretching), 1493.20(-N-C=S stretching),

1237.17(C=S stretching), 1074.43 (C-N stretching). 1H NMR (400 MHz CDCl3 δ ppm), -

NH proton at δ 8.2035-8.1035 ppm, Ar-H protons at δ 7.6123-6.6618 ppm, phenolic-OH

proton at δ 5.3784 ppm, NH proton at δ3.6417 ppm and CH3 protons at δ 1.4456-1.2922

ppm. 13

C Spectrum: C=S carbon at 183.69-181.14 ppm, Ar-C carbon at 140.46-120.91

ppm, -CH3 carbon at 40.06-38.80 ppm. Mass analysis:Fragmentation occurs during the

analysis is given

OH

NH NH

S

CH3

M+= 182.24

OH

NH NH2

S

168.21

OH

NH

S

OH

94.11

153.20

From above chemical characteristics, elemental and spectral studies compound (IIIb)

was assigned structure as N‘-(2-hydroxyphenyl)-N‖-methylthiourea.



OH

NH NH

S

CH3

2-Methylthiocarbamidophenol

(IIIb)

Similarly,N‘-(2-hydroxyphenyl)-N‖-phenylthiourea(IIIb)N‘-(2-hydroxyphenyl)-N‖-(4-

chloro)phenylthiourea(IIIc) and N‘-(2-hydroxyphenyl)-N‖-tolylthiourea(IIId) were

synthesized by interacting of 2-aminophenol with phenylisothiocyanate(IIa), p-

chlorophenylisothiocyanate(IIc) and p-tolylisothiocyanate(IId)respectively by

abovementioned method and enlisted in Table No. 2.1.

Table No. 2.1

Sr.No. N‘-(2-Hydroxyphenyl)-N‖-substitutedthioureas Yield (%) m.p.(0C)

1. ------phenyl------------------- 9 189

2. -----(p-chlorophenyl)------ 91 191

3. -----(p-tolyl)----------------- 89 203

Reference:

1. Varma R.S., ACS Symposium ― Green Chemical synthesis and Processes , Chap 23,

Pg 292-313, American Chemical Society , Washington D.C., 2000.

2. Verma R.S., ― Green Chemistry , Challenging Perspective‖ Oxford University Press

, Oxford , Pg 221, 2000.

3. Verma R.S., ―Microwaves in Organic Synthesis, Chap. 6, Wiley–VCH, Weinheim ,

PP 181, 2002.

4. Doble M. and Kruthiventi A.K., Green Chemistry and Engineering, Academic Press,

2007.


6. Strauss C. and Varma R., Microwaves in Green and Sustainable Chemistry,

Microwave Methods in Organic Synthesis, 199-231, 2006.

7. Fini A. and Breccia A., Chemistry by Microwaves, Pure Appl. Chem., 71(4), 1999,

573-580.

8. Larhed M. and Hallberg A., Microwave-Assisted High-Speed Chemistry: a New

Technique in Drug Discovery, Drug Discovery Today, 6(8), 2001, 406-416.



9. Lidstroem P., Tierney J., Wathey B. and Westman J., Microwave Assisted Organic

Synthesis: a Review, Tetrahedron, 57(45), 2001, 9225-9283.

10. Caddick S., Microwave Assisted Organic Reactions, Tetrahedron, 51(38), 1995,

10403-10432.

11. Kappe C.O., Controlled Microwave Heating in Modern Organic Synthesis, Angew.

Chem. Int. Ed., 43(46), 2004, 6250-6284.

12. Loupy A., Petit A., Hamelin J., Texier-Boullet F., Jacquault P. and Mathe D., New

Solvent-Free Organic Synthesis using Focused Microwaves, Synthesis, 1998(9),

1998, 1213-1234.

13. Varma R.S. ,Microwaves : Theory and application in material processing IV,

American chemical society , Westerville , Ohio, 1997, PP 357.

14. Chnlak I., Sntorins V. and Sederka V., Chem.Pap.,44, 1990, 131.

15. Papenfnws T., Ger.offen.De.,3, 1987, 528.

16. Shingare M.S. and Ingale D.B, J. Ind. Chem.Soc.,53, 1976, 1036.

17. Dash B. and Patra M., Indian . J. Chem., 19B, 1980, 894.

18. Lewis J. and Wilkins R.G., Modern Coordination Chemistry, Inter Sci. pub. Co.,

New York, 1960.

19. Vogel A.I., A Text Book of Qualitative Inorganic analysis, 3rd Ed., ELBS Edition

first published 1962 Reprinted 1968.

Microwave assisted synthesis and anti-microbial evaluation of some novel 2-

aminopyrimidines”

D. T. Tayade1*, S.A.Waghmare

2

1 Department of Chemistry, Government Vidarbha Institute of Science and Humanities, Amravati

444 4, Maharashtra State, India. 2 Dept. of Chemistry, G.N. Azad College, Barshitakli, Dist. Akola -444 401, (M.S) India.

* Corresponding author - [email protected], [email protected]

Abstract:

Present research reveals microwave assisted synthesis of 4-(substituedphenyl)-6-(

substituedphenyl)pyrimidin-2-amine(IIIa-e) by the interaction of (2E)-1-(substituedphenyl)-

3-(substituedphenyl)prop-2-ene-1-one(Ia-e) and guanidine hydrochloride (II) in 25%

sodium-ethoxide and ethanol. All the synthesized compounds were characterized by spectral

data (FT-IR, MS, H1NMR) and antimicrobial activities are evaluated against gram-+ve

(staphylococcus) and gram—ve (e-Coli).

Key Words: 2-Aminopyrimidine, guanidine hydrochloride, sodium-ethoxide and ant

microbial activity.




Introduction:

A survey of literature revels that very little work has been carried out on the synthesis

of substituted pyrimidine compounds and their derivatives. The pyrimidine moiety is

particularly very interesting in the area of medicinal and organic chemistry. The synthesis of

substituted pyrimidine and their derivatives has a very high drug score. These heterocyclic

compounds now find a wide variety of applications ranging from anticonvulsant,

antitubercular, antimycobacterial, antiasthematic, antipyretic, neuroprotective to antinephritic

activities. These considerable biological activities have been stimulated interest in the

synthesis and chemistry of new class of pyrimidine derivatives.

Recently, it‘s a big challenge before the chemist to synthesize the biologically potent

heterocycles by the greener way with reduction of hazardous chemicals and less time

consumable method1-5

. Modern organic synthesis involves the sono, grinding, use of natural

products and microwave4-6

. The most effective, less time consumable and greener way is the

microwave assisted sythesis6-7

.

In this method, we report the novel heterocyclic system possessing fused rings. The

compound 4-6-(disubstituted)pyrimidine-2-amine was prepared from the chalcone (2E)-1-3-

(disubstituted)prop-2-ene-1-one with reagent guanidine hydrochloride under microwave. The

chalcone (2E)-1-3-(disubstituted)prop-2-ene-1-one was prepared by the reaction of

substituted benzaldehyds, heterocyclic benzaldehydes and substituted acetophenones under

microwave.

REACTIONS:

HO

+

O O

R1

R2R

1 R

2

20% NaOH

Ethanol

Scheme-I

Microwave

O

R1

R2

N N

NH2

R1

Guanidine hydrochloride

25% Sodiun Ethoxide

Scheme-II

R2

Microwave

Where R1=H, 2-OH, (OCH3)2 ,3-NO2, Py-2-CHO

R2 = H, 4-Cl, 4-NO2

, 2-NH2 ,4-OH

Experimental work:

A] Synthesis of (2E)-1-(4-chlorophenyl)-3-(3,4-dimethoxyphenyl)prop-2-ene-1-one:



Interaction of 3,4-dimethoxybenzaldehydes and 4-chloroacetophenone carried under

microwave for 2min. The stirred mixture was then poured into ice cold water and acidified

for neutral. The mixture was then filtered and recrystalised from ethanol. The purity of

compound was checked by TLC. Conformation of chalcone by Conc.H2 SO4 was dropped

into chalcone product it gives red-brown color which indicate formation of chalcone (Wilson

Test).

B] Synthesis of 4(4’-chorophenyl)-6-(3,4-dimethoxyphenyl) pyrimidine-2-amine:

Interaction of (2E)1-(4-chlorophenyl)-3-(3,4-dimethoxyphenyl)prop-2-ene-1-one was

carried with guanidine hydrochloride in r microwave for 3min. Product obtained was poured

in ice cold water and made the solutions neutral with drop of dil. HCl. The solution was

filtered and dried. The purity of compound was checked by TLC. The free amino group of

product was also checked by diazotization test, laboratory conformation test for amino group.

(Compound +1mL of HCl cool to 0OC+2-3 drops of NaNO2 + β-napthol in NaOH gives Red

dye formation).

Result & discussion:

Reaction data, spectral analysis and anti-microbial activities of the synthesized

compounds are shown below:

A] Synthesis of (2E)-1-(4-chlorophenyl)-3-(3,4-dimethoxyphenyl)prop-2-ene-1-one:The

structure of the compound was assigned on the basis of elemental analysis of spectral data.

[IR (KBr): 2835,2931 cm-1 (-CH), 1681cm-1 (C=O),1568 cm-1(C=C).

Melting point-1050C Yield-73%

Mol.Wt.-302.5 Mol.Formula-C17H15O3Cl

B] Synthesis of 4(4’-chorophenyl)-6-(3,4-dimethoxyphenyl)pyrimidine-2-amine: The

structure of the compound was assigned on the basis of elemental analysis of spectral data.

[IR (KBr): 3448,3367 cm-1 (-NH), 2850 ,2926cm-1 (C-H),1778 cm-1(C=N) ,1139 cm-1(C-

O-C) ; 1H NMR(DMSO): 3.4(s, 2H, NH2), 5.8(s, 1H,Ar-H), 6.8(d,d 2H, Ar-H), 6.6(d,d 2H,

Ar-H) , 6.4(s, 1H,Ar-H), 6.5(d, 1H,Ar-H),3.8 (s,6H, -OCH3); Mass: M+ m/e 341.5].

Melting point-2700C Yield-52%

Mol.Wt.-341.5 Mol.Formula-C18H16N3O2Cl

Anti Microbial Activities:

Results obtained by the anti-microbial activities of the synthesized compounds were

screened against gram +ve s-auerios and gram -ve e-coli is shown along with standard

amphicilin drug.



COMPOUND Diameter zone of inhibition(mm)

gram +ve s-auerios gram -ve e-coli

A 13mm 15mm

B 14mm 16mm

C 18mm 22mm

Standard

ampicilin

32mm 34mm

References:

1. Strauss C. and Varma R., Microwaves in Green and Sustainable Chemistry,

Microwave Methods in Organic Synthesis, 199-231, 2006.

2. Doble M. and Kruthiventi A.K.,Green Chemistry and Engineering, Academic Press,

2007.

3. Fini A. and Breccia A., Chemistry by Microwaves, Pure Appl. Chem., 71(4), 1999,

4. Lidstroem P., Tierney J., Wathey B. and Westman J., Microwave Assisted Organic

Synthesis: a Review, Tetrahedron, 57(45), 2001, 9225-9283.

5. Verma R.S., ―Microwaves in Organic Synthesis, Chap. 6, Wiley–VCH, Weinheim ,

PP 181, 2002.


7. Larhed M. and Hallberg A., Microwave-Assisted High-Speed Chemistry: a New

Technique in Drug Discovery, Drug Discovery Today, 6(8), 2001, 406-416.

8. Caddick S., Microwave Assisted Organic Reactions, Tetrahedron, 51(38), 1995,

9. Verma R.S., ― Green Chemistry , Challenging Perspective‖ Oxford University Press ,

Oxford , Pg 221, 2000.

10. Vogel A.I., A Text Book of Qualitative Inorganic analysis, 3rd Ed., ELBS Edition first

published 1962 Reprinted 1968.

11. Pavia D. L., Lampman G. M. and Kriz G. S., Introduction to Spectroscopy, 3rd

Edition

(Indian Edition), Harcourt College Publishers, Orlando FL, 2006.

12. Dyer J. R., Application of absorption spectroscopy of organic compounds, 8th

Edition,

John Wiley and Sons, Inc, New York, 1997, 27.

13. Sharma Y. R., Elementary Organic Spectroscopy: Principles and Chemical

Applications, 4th

Edition, S. Chand and Company Limited, New Delhi, 2012.

14. Kalsi P. S., Spectroscopy of Organic Compounds, 6th

, New Age International

Publishers, Delhi, 2010.



Green Methodology For Determination Of Pharmacodynamics Of Chemical Molecules

A. B.Wadekar1,

D.T.Tayde

2*

1Department of Chemistry,S.D.M. Burungale Science and Art College, Shegaon, 444203.

2Department of Chemistry, Government Vidarbha Institute of Science and Humanities, Amravati

444604.

* Corresponding author [email protected], [email protected],

Abstract: The medicinal properties of any molecule are checked after synthetic process for that

various physical methodologies are used. The medicinal pharmacological, anatomical and

physiological activities are established on the basis of pharmacodynamics and

pharmacokinetics of that molecule. In pharmacodynamics there are four steps i.e. absorbance,

diffusion, metabolism and execration, in first three steps the molecule must be stable and its

stability can be determined by its stability constants. Hence considering these facts

conditional stability constant of5-phenylthiocarbamido-1-naphthol or (L2) and 5-p-

tolylthiocarbamido-1-naphthol (L4)) with Cu (II), Cd (II) and Cr (III) metal ions and

formation of complexes in 70% ethanol-water media at different proportions were

investigated spectrophotometrically. This investigation helps to understand drug effect and

drug activity of newly synthesized drugs.

Keywords: 5-p-Tolylthiocarbamido-1-naphthol,stability constant and spectrophotometric.

Introduction

Determination of anatomical, physiological and pharmacological activities of any

molecule is essential for knowing its significances and application in medicinal,

pharmaceutical agricultural and industrial sciences. One of the important property in these

studies is to establish drug activity drug effect, transmission of drug and absorption of drug

all these four factors are directly depend stability of the molecule. The stability of molecules

during this study can be easily determined from the values of stability constants. The

formation of complex can also be determined from these values, hence spectrophotometric

measurements created its own importance in life, medicinal and pharmaceutical, agricultural

and industrial sciences. Physical and chemical properties are varied due to complexation.

Composition as well as conformation of complex formation can be measured from study of

various physicochemical properties by spectrophotometric method. Spectrophotometric

technique has a great significance in measurements of stability constant and confirmation of

complex formation in solution. Wagh [1] and Deshamukh [2] determined log K value of

chalcones pyridine carboxylic acids and hydroxyl ethyl benzene. Galhan et al[3]studied (E)-

2-(mercaptophenylaminoethylene)-3-oxo-N-p-tolylbutamide with some metal ion by

spectrophotometrically. Boldescu et al [4]Spectrophotometrically studied sangurine-

bcyclodextrin complex formation. Spectrophotometrically determination of

phenylprinehydrochloride and salbutamol sulphate drugs in pharmaceutical preparation using

diazotized metacloprine hydrochloride was carried out by Al-Abachi and Abed[5].




Alsamarrai et al[6] investigated ephedrine-hydrochloride by spectrophotometrically.Saleha et

al[7]investigated sulphsalazine antibiotics drugs. Investigation of ion complex formation of

anti-hypertensive drug mehtyldopal was studied [8]. Meshram[9] studied complexation by

interaction of Dy(III) with lincomycine and lyrodoxin in 70% ethanol-water medium.

Spectrophotometric study of diflunisalfebuxostatemetaxalone, fexofenadine methyl ester and

linezolide pharmaceutical dosages using tetracynoethelene was carried out by Shrinivas et al

[10] . Valtierra–Alvardoet al[11] investigated complex formation equilibrium of Cu(II).

Solvent effect on dissociation of ammonium and pyridiniumion was studied by Ohataki[12].

Investigation of effect of dielectric constant on Cu(II) –Complexes of phthalic acid in various

percentage of dioxane-water mixture was carried by Palaskar[13].Metal-ligand stability

constant and confirmation of complexes formation of 5-p-Tolylthiocarbamido-1-naphthol

with Cu(II), Cd(II) and Cr(III) metal ions had been investigated respectively by

Spectrophotometric technique at 0.0001 M ionic strength. This work is mainly base on Jobs

method of continuous variation. It is specially associated to study of effect of solvents, effect

of ligands and group as well as effect of metal ions during formation of complexes.

Expermental:

5-p-Tolylthiocarbamido-1-naphthol has been synthesized in the laboratory by standard

method. The nitrate salts of copper cadmium and chromium were used and their solutions

were prepared in double distilled water. The solutions of potassium nitrate was prepared (1M)

and used for maintaining ionic strength constants. Absorption are measured by UV

Spectrophotometer model 106, (Systronic make) with an accuracy = ± 0.005 was used.

Results And Discussion:

Jobs method of continuous variation method is reliable method for investigation of

formation of complex [14]. Jobs method consist of equimolar solutions of metal and ligand

varying proportion in such manner that total concentration of metal plus ligand is constant in

resulting mixtures[15]. The compositions of metal ions solution (1 x10-4

M) and ligand (5x 10-

4M) were prepared in ten series. Ionic strength was maintained constant (0.1M) by adding an

appropriate amount 0f 1M KNO3 solution in 10 ml volume (λmax) was determined using one

of the compositions at which there is maximum absorption. The absorption for all the

compositions was recorded at a constant wave length (λmax). The data of absorption and %

composition of metal ion and ligand solutions at constant pH can be used to construct the

curves. It was observed that 1:1 complex formation occurs in the pH range of 3 to 6. Each

solution is diluted up to 15 ml and recorded absorption at same (λ max). Conditional stability



constants of metal ligand complexes were calculated for all the systems using following

expression.

X X

K = ----------------- = -------------------

(a1-x)(b1-x) (a2-x)(b2-x)

K = Conditional stability constants of complex. X = Concentration of complex.

a1and a2 = Concentration of metal ions; b1 and b2 = Concentration of ligand.

Conditional stability constants of metal ligand complexes were calculated and presented In

Table-1

Table-1: Determination of Conditional Stability of Metal Ligand Complexes

System Conditional stability constant Log K

Cu(II)+ L4 5.2911 X10-3

0.72354 X 10-3

Cr(III)+ L4 3.4732 X 10-3

0.54072 X 10-3

Cd(II)+ L4 2.0833 X 10-3

0.31875 X 10-3

Conclusion

From Table 1 it was conclude that resultant values obtain in both techniques are fairly

good. There is no appreciably change in log K values. This indicated the simultaneously

complex formations. Variation in Log K values observed due to direct interfere of dielectric

constant, solvent-solvent interaction, solute-solvent interaction and solute-solute-solvent

interaction. Table 1 revel that Log K value of L4 greater for Cu(II) than Cd(II) and Cr(III).

Thus L4form more stable complex with Cu(II) than Cd(III) and Cr(III).This investigation

helps to pharmacodynamics and pharmacokinetics of the study of drug activity and drug

effect of newly synthesized drugs.

References

1. S.P.Wagh, Metal-ligand stability constant value of chalcones pyridine carboxylic

acidsPh.D. Thesis Amravati University, Amravati (2004).

2. C.N. Deshmukh, M-L stability constant of chalconeshydroxyl ethyl benzenePh.D.

ThesisAmravati University, Amravati (2004).

3. A.A. Gahlam, A.Y. El-Sayed, M.A. Marouf and M.M.Taufij, To study of (E)-2-

(mercapto-phenylamino ethylene)-3-oxo-N-p-tolylbutamide with some metal ion by

spectrophotometricallyInt. J. of Advance Research 3(1) (2015) 630-642.

4. VeaceslavBoldescu. , Irina Kacso, IoanBratub and Gheorghe Duca, to study the

sangurine - bcyclodextrin complex formation, Chemistry J. of Moldova 3(1) (2008)

85-88.



5. Mouyed Q, Al-AbachiandSadeem S.Abed, Spectrophotometrically determination of

phenylprine hydrochloride and salbutamol sulphate drugs in pharmaceutical

preparation using diazotized metacloprine hydrochloride Baghdad Science J., 12(1)

(2015)

6. KhalafF.,Alsamarrai, Sarmad B.Dikram, MuminF.Alsamarrai, spectrophotometrically

study of ephedrine-hydrochloride, International journal of science technology and

management4(1) (2015) 1738-1746.

7. Magda M. S. Saleha, Elham Y. Hashem,Ahmed K. Youssef and Doaa A. Abdel-

Kadir, study of sulphsalazine antibiotics drugs, World Journal of pharmacy and

pharmaceutical science, 4(5) (2015) 205-226.

8. TehminaFiaz, NasreenFatimal, S. Zafar Abbas Zaidi,Tanveer Abbas Mohib R.

Kazimi, Investigation of ion complex formation of anti-hypertensive drug

mehtyldopal was studied, American Journal of Analytical chemistry 6 (2016), 551-

558.

9. K. Meshram, studied complexation by interaction of Dy (III) with lincomycine

andlyrodoxin in 70% ethanol-water, Ph.D.thesis in chemistry Amravati university,

Amravati, (2000).

10. B. Shrinivas, P.Yadagoroswami and G. Venketeswarlu, Spectrophotometric study of

diflunisalfebuxostatemetaxalone, fexofenadine methyl ester and linezolide

pharmaceutical dosages using tetracynoethelene, International Journal of

Pharmaceutical Science and Research 6(6) (2015) 1002-1010.

11. M. A. Valtierra-Alvarado, M.PamelaSolano-Garcia, Maria del RefigioGonzalez-

Ponce, Jose J.N. Segoviano-Garfis, Complex formation equilibrium of Cu(II),

International Journal of Science and research publication, 5(6) (2015) 1-8.

12. H. Ohataki, Solvent effect on dissociation of ammonium and pyridinium Bull.

Chemical Society of Japan, 42(1969) 1573.

13. N.G.Palaskar ,effect of dielectric constant on Cu(II) –Complexes of phthalic acid in

various percentage of dioxane-water mixture, Ph.D. Thesis Marathvada University,

Aurangabad1971.

14. M. M. Krunz and L. B. P. Fendth,Complex formation by Jobs method,

Microchemistry Journal, 28 (1983) 162.

15. P. B. Raghuvanshi, A.G. Doshiand M.L. Narwade, Journal Indian Chemical Society

73 (1996) 21.



Plant Assisted Plant Assisted ZnO nanoparticle as catalyst for efficient green one-pot

synthesis of benzimidazole derivatives .

aGurumeet C. Wadhawa,

aVitthal S. Shivankar ,

aYashwant A. Gaikwad,

bCharansingh

H Gill, bLaxman V. Gavali

aPost Graduate Department of Chemistry, Karmaveer Bhaurao Patil College Vashi

Navi Mumbai, 400703, Maharashtra, Indian bDepartment of chemistry Babasaheb Ambedkar Marathwada University Aurangabad

Abstract. Green chemistry protocols with there usability of then an oparticle as catalys tin

the synthesis of benzimidazole is described in this work.Thezincoxide (Plant Assisted ZnO

)nano particles functions ashighly effective catalyst for the reactions of various substituted

Benzimidazoles with ortho-phenyldiamine unders on ication conditions to afford the

corresponding benzimidazole inmoderateto goodyields. The catalyst is inexpensive, stable,

can be easily recycle dandreused for several cycles with consistent activity.

Keywords- Benzimidazole derivatives, zincoxide(Plant Assisted ZnO) ;catalyst; green

chemistry .

Introduction

Intensive studies have been recently focused on the development of catalytic system

showing to their importance in s yn t h e t i c organic c h e m i s t r y . One o f t h most

attractive synthetic strategies favoured by organic chemists is the use of heterogeneous

catalyst in increasing the efficiency of a wide range of organic synthesis.

Heterogeneous catalysis is being use the fine- chemicals industry because of the need formore

environmentally friendly production technology.This tendency is assisted by the availability

of novel catalytic materials and modern technique sofcreating and investigating specific

active sites on catalyst surfaces.1,2

Inthefieldoffinechemicalproduction, important steps in the

synthesis of relatively large and complex molecules include carbon–carbon bond forming

reactions such as synthesis of benzimidazole derivaties, Knoevenagel condensation etc..The

synthesis of benzimidazole derivatives,isoneofthemost useful and widely employed

method for carbon– carbon bond formation ino rganic synthesis, with numerous

applications in the synthesis of fine chemicals,4

andcarbocyclic as we llashet- erocyclic

compound so biological significance.5

The reactionsareusuallycatalyzedby6

suchas avid, various

lewis acid,pyridine,ammoniaor sodiumethoxideinorganic solvents.In recent years,metaloxides

constitute the largest family of catalyst in heterogeneouscatalysis7–10

due to their acid-base and

redox properties. Recently, bulk Zincoxide has been employed as a heterogeneous catalyst

for various organictransf ormations.11

The recent literature survey reveals that nano size Plant



Assisted ZnO 12

as heterogeneous catalyst has received considerable attention because ofi

tsinexpensive,non-toxiccatalystand has environmental advantagesi. minimum

executiontime,lowcorrosion,wasteminimization,recyclingofthecatalyst,easytransportanddispos

alofthecatalyst.Inrecentyears,inbiologicalfieldthepotentialutility ofPlant Assisted ZnO

nanoparticleinthetreatmentofcancerhavebeenreportedbymanyresearchers.Owingtonumerousadva

ntagesassociatedwiththiseco

2. Experimental

All chemicals employed were commercial products (S.D.Fine Chemical Co) and were used

without purification. All yields refer to isolated products after purification. 1H (300 MHz)

NMR and 13C (75 MHz) NMR spectra wererecorded on Varian mercury XL-300 and Bruker

spectrometer instruments using TMS as internal standard. Thesolvent used for NMR spectra

was CDCl3

and DMSO-d6. Infra red spectra were taken on Shimadzu FTIR–408 inKBr.

. Column chromatography was performed on silica gel (230–400 mesh) supplied by Merck

General Procedure

In a typical run, o-phenylene diamines (10m mol), aryl aldehydes (10m mol) and Plant

Assisted ZnO nanoparticles (0.8 mol) were grinded in mortar and pestle at room temperature

for 25 minutes. Add 10 ml water to reaction mixture and filter product on filter paper and

recrystalize by using suitable solvent. Recovered catalyst was dried and reused further in

successive reactions. Filtrate was collected and evaporated under reduced pressure to afford

the catalyst. The products obtained were confirmed by IR and NMR.

NH2

NH2

+

O

N

NH

zno nano particles

RT sonicator

Spectral data of selected compounds

2-Phenyl-1H-benzimidazole ): FT-IR (KBr): 3500, 1718, 1600, 948, 740 cm-1; 1H NMR

(300 MHz, DMSO-d6): δ 7.20 - 8.20 (m, 4H), 7.46-7.62 (m, 5H), 12.02 (s, 1H, NH);

13C NMR (75 MHz, DMSO-d6) : δ 115.16, 139.17, 129.97, 129.06, 128.88, 126.50, 122.16,

151.03 ;

2-(4-chlorophenyl)-1H-benzimidazole :FT-IR (KBr): 3548, 1722, 1600, 1450, 1550, 748

cm-1; 1H NMR (300 MHz, DMSO-d6): δ 7.21-8.18 (m, 4H), 7.53 (d, J = 8.4 Hz, 2H), 7.66

(d, J = 8.4, 2H), 12.94 (s, 1H, NH);



13C NMR (75 MHz, DMSO-d6) : δ 115.53, 119.06, 121.90, 122.67, 126.74, 127.81, 129.86,

135.30, 139.90, 144.82, 152.73;

2-o-tolyl-1H- benzimidazole

1H NMR (DMSO-d6): δ7.82-7.79 (m, 3H), 7.60-7.58 (m, 1H), 7.56-7.45 (m, 4H), 2.58 (s,

3H);

2-p-tolyl-1H-- benzimidazole

1H NMR (DMSO-d6): δ12.81 (br s, 1H), 8.06 (d, J=8 Hz, 2H), 7.56 (m, 2H), 7.36 (d, J=8

Hz, 2H), 7.19 (m, 2H), 2.38 (s, 3H);

Results And Discussion

Sr. no. Aldehyde Time in min % yield M.P.0

C

1 Benzaldehyde 25 95 290

2 2methyl bezaldehyde 30 90 196

3 4 methylbezaldehyde 30 70 225

4 2 chlorobezaldehyde 22 78 231

5 3chlorobezaldehyde 27 78 233

6 4 chlorobezaldehyde 22 68 291

7 2 nitrobezaldehyde 20 88 121

8 3nitrobenzadehyde 20 80 309

9 4nitrobenzalehyde 20 93 291

10 2 hydroxybenzaldehyde 40 76 256

11 4 hyderoxybenzaldehyde 39 77 208

12 4flurobenzaldehyde 20 67 250

13 Cinnamaldehyde 27 88 200

14 Furfuraldehyde 25 79 96

In our preliminarily investigation on the model reaction of o-phenylenediamine and

benzaldehyde, it was found that the reaction could be finished under very simple reaction

conditions in the presence of catalytic amount of zinc oxide nano particles which gives the

desired 2-phenyl benzimidazole product in good time and yield. The reaction was

systematically investigated and the results are represented in table 1.

Herein, we wish to disclose a novel protocol for the rapid synthesis of a variety of

biologically significant benzimidazoles using a catalytic amount of Plant Assisted ZnO

nanoparticlesunder optimized reaction conditions. As shown in Table- different aldehydes



and o-phenylenediamine react without any significant difference to give the corresponding

benzimidazoles in good yield. When the mole ratio of o-phenylenediamine and aldehyde are

taken in 1:1 ratio the product 2-substituted benzimidazoles were obtained as selectively. It

indicated that zinc oxide nano particle catalyzed reaction has a favorable selectivity for the

synthesis of 2-substituted benzimidazoles.

benzimidazole was also formed as a major along with 2-substituted benzimidazole.

The reusability of the catalyst is an important factor from economical and environmental

point of view and has attracted much attention in recent years. Therefore, the reusability of

zinc oxide nanopartices was examined in the reaction of simple benzaldehyde with o-

phenylenediamine under optimized reaction conditions. As Plant Assisted ZnO

nanoparticlesis a heterogeneous catalyst, it was separated by simple filtration after dilution of

reaction mixture

. The filtered catalyst was dried at 100oC and reused. In first time we used fresh catalyst to

give 95% yield, after second time reuse we got 88% yield and also we tried for third time

reuse to get 71% yield under noptimized condition. The results showed that the catalyst can

be used 3 times without much loss of its activity.

Conclusion

The use of triethanolamine has asignificantinfluenceon the morphologyof Plant Assisted

ZnO.Wehavedevelopedanefficient,acileandenvironmentallyacceptablesynthetic methodology

for the synthesis of benzimidazole derivatives using nano-Plant Assisted ZnO

catalystundersolvent-free condition.The advantage so environmentally benig

nandsafeprotocol include as implereaction set up,very mild reaction conditions,high product

yields,short reaction times,and the possibility for reusing the catalyst, chemoselectivity and

solvent-free conditions.

References

1 Corma A. Chem Rev, 95: 559,(1995).

2.S.Robert, I.M. McDonald, Burger‘s Medicinal Chemistry & Drug Discovery, 6th

ed;

JohnWiley and Sons, New Jersey, (2003).

3.Avinash Patil, Swastika Ganguly and Sanjay Surana ,Rasayan J. Chem 1,3,P. ( 2008.)

4. Clark J H. Pure Appl Chem, ,73,103, (2001)

5. Chen C, Chen Y. Tetrahedron Lett, ,45, 113,(2004).

6 .Patil A, Ganguly S, Surana S. J Chem Sci, ,122, 443, (2010)

7.Valdez J, Cedillo R, Hernandez-Campos A, YepezL,Hernandez-Luis F, Navarrete-Vazquez G,

Tapia A, Cortes R,Hernandezc M, Castilloa R. Bioorg Med Chem Lett, , 12:22,(2002)



8. Fonseca T, Gigante B, Gilchrist T L. Tetrahedron, ,57:1793, (2001)

9 .Wang Y-C, Liu L-Z, Pan Y-M, Wang H-S. Molecules, ,16: 100,(2011)

10. Strenbach L H. J Med Chem, 22: 1,(1979),

11. Schultz H. Benzodiazepines. Heidelberg: Springer, (12,19,1982.)

12. Randall L O, Kappel B, Garattini S, Mussini E, Randall L Oeds. Benzodiazepines. New York:

Raven Press, (1973. 27).

Eco-friendly set up for determination of stability constants of 4-(p-tolyl)thiocarbamido-

phenol complex of Cu(II), Cd(II), Co(II) and Ni(II) metal ions in 70% ethanol-water

system N. J. Meshram

a, D.T.Tayade

b*, K. D.Tayade

c , G.D.Tayade

d

a Department of chemistry, S.R.R.L. Science College Morshi, Maharashtra, India.

bDepartment of chemistry, Govt. Vidarbha Institute of Science and Humanities, Amravati,

cJ.S.P.M. Imperial College of Engineering and Research,Wagholi, Pune Maharashtra

d Department of Physics, Govt. Vidarbha Institute of Science and Humanities, Amravati,

Theoretical determination of anatomical and physiological activities of any molecule is

essential for knowing its significances and application in medical science.

Pharmacodynamics and pharmacokinetics are directly dependent on stability of a molecule.

The stability of molecules can be easily determined from the values of stability constants and

formation of complex can be also demined from this. Considering this eco-friendly pH-metric

method was used for determination of values of proton-ligand stability constant and metal-

ligand stability constants for 4-(p-tolyl)thiocarbamidophenol with Cu(II), Cd(II), Co(II) and

Ni(II) metal ions at 0.1 M ionic strength in 70 % ethanol-water mixture. This method is

handy, simple and having easy work up. Results obtained can be easily cross verified.

Keywords: 4-(p-tolyl)thiocarbamidophenol, stability constant, pH-metry.

References [1] D. M. Barnes, Ji. Jianguo, M.G. Fickes, J. Am. Chem. Soc., 2002, 124, 13097-13105.

[2] V. Cyril, M. Milam. Chem. Abstr., 1977, 86, 190015.

[3] A. G. Ghaigy. Siess Patent, 1965, 393, 344.

[4] C. D. Bossinger and E. Tekeshi. Chem. Abstr., 1972, 77, 343590.

[5] M. Seidal, F. E. Betiver. African Patent, 1964, 68, 03, 47.

[6] H. Irving and H. Rossotti. J. Chem. Soc., 1954, 2904.

[7] A. E. Martell and M. Calvin. Chemistry of metal chelate compounds. Prentice Hall. Inc. England.

Cliffs. N. J. 1962.

[8] A. K. Banarjee and T. V. R. Rao. J. Indian Chem. Soc., 1968, 63, 480.

[9] YK Meshram; RF Khan; Ind. J. Aplli. Res., 2014, 4(3), 37.

[10] VB Khobragade; ML Narwade; JCPR, 2013, 5(10), 189.



Spectoscopic analysis of some medicinal plants with some infected blood samples.

Supriya Kate and Harsha Chathrath

Research Scholar, JJT University Rajasthan

Adjunct Professor, Dr. D.Y. Patil College, Pune

spectroscopic techniques can be effectively employed as a diagnostic tool in clinical

chemistry and it can be an alternate method in clinical analysis. The present work is to study

the spectral difference in the spectrum of different medicinal plants and their combinations

with infected blood samples like dengue and viral blood by UV-VIS spectroscopic technique.

This can be used for diagnosis of the diseases like malaria, dengue, and viral.

Key words: Infected blood samples, medicinal plants, UV-Visible spectroscopy.

Ultrasonic studies of 2-Aminothiazole at different temperatures.

A.B. Naik1, P. B .Morey

2, A.B.Bhagwatkar

3 and S. U. Patil

4

1Department of Chemistry, Vidya Bharti College, Karanja Lad (MS) INDIA

2Physical Chemistry Laboratory, Department of Chemical Technology, SGB Amravati

University, Amravati-444602 (M.S.) India 3 Jagrut College, Warud

4Department of Chemistry, Institute of Science, Nagpur

Fax. +91-721-2662135 Tel. +91-721-2668090 e-mail: [email protected]

Measurements of physicochemical properties such as density and ultrasonic velocity of pure

components, binary and ternary mixtures have been used for investigations of thermo-

acoustical parameters of pure liquids and their mixtures. In present study physicochemical

properties of liquids mixtures namely, 2-Aminothiazole + 1, 4-Dioxane (Dx)-water have been

estimated at 303.15K, 308.15K and 313.15K for the entire range of molar concentrations.

From the experimental data, some acoustical parameters viz. adiabatic compressibility (βs),

intermolecular free length (Lf), specific acoustic impedance (Z) and relative association (RA)

have also been calculated using standard relations. The result suggests presence of molecular

interactions in components. The effect of different temperatures on strength of molecular

interaction has also been studied.

Keywords: Density, ultrasonic velocity, acoustical parameters, solute-solvent interaction.




Sonochemical synthesis of La-doped ZnO nanostructures and their photocatalytic

activity

S. S. Handea, V.S.Astekar

a, S.P.Chaudhari

b, D.T.Tayade

c, S.P.Meshram

d, P.D.Jolhe

a*

aDept. of Biotechnology, Singhgad College of engineering, Pune -411 041

bNanotechnology Research Laboratory, Dept. of Chemistry, Shri Shivaji Sci. College, Amravati-

cDept. of Chemistry, Govt. Vidarbha Institute of Science & Humanities, Amravati-444 604

dCentre for materials for electronics technology (C-MET), Pashan, Pune- 411 008

Corresponding author mail: [email protected]

Present paper reports facile sonochemical approach for synthesis of La-doped ZnO

nanostructured materials. The products were characterized by different characterization

techniques viz. X-ray diffraction (XRD), field emission scanning electron microscopy

(FESEM), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), particle

size distribution analysis and UV-visible spectroscopy. XRD patterns of pure ZnO and La-

doped ZnO are specified as hexagonal wurtzite ZnO structure with no detection of

La2O3 phase. FESEM characterization revealed that, the as-synthesized products are having

vesicular morphology with size ranging from 20-30 nm. Photocatalytic activities of the as-

synthesized products were determined by measuring the degradation of methylene blue

(MB) under sunlight irradiation. Among them, the 2.5 mol% La-doped ZnO shows best

photocatalytic properties.

Keywords: Sonochemical, La-doped ZnO, photocatalytic activity

Undoped ZnO 0.5 mol% La-doped ZnO

1.5 mol% La-doped ZnO 2.5 mol% La-doped ZnO

3.5 mol% La-doped ZnO



Ultrasound Assisted Synthesis Of Isoxazolone Derivatives Using Ionic Liquid As An

Efficient And Green Catalyst.

Gopinath D. Shirolea,b

, Adinath S. Tambea, Sharad N. Shelke

b*

aDepartment of Chemistry, A.S.C. College, Rahata, Dist-Ahmednagar (MH) 423107,

bDepartment of Chemistry, S.S.G.M. College, Kopargaon, Dist-Ahmednagar (MH) 423601,

*Corresponding author. Tel.: +918888199853. E-mail address: [email protected]

A series of Isoxazolone derivatives were efficiently synthesized by environmentally

benign, one-pot three component condensation of various aldehyde, β-keto ester and

hydroxyl amine hydrochloride in the presence of Ionic Liquid as a catalyst in ethanol. These

derivatives have been synthesized by conventional as well as ultrasonication. This junction of

Ionic liquid and Ultrasound waves makes the protocol environmentally benign. Also it has

several benefits such as simple work-up procedure and good yields. The formation of

compounds was confirmed on the basis of their IR, 1H-NMR,

13C-NMR and mass spectral

techniques.

Keywords: Isoxazolone, multi-component strategy, Ionic liquid, etc.

Synthesis, Characterization and Biological Activities of Macrocyclic Ligand.

S. A. Najan Department of Chemistry, Arts, Commerce and Science College Sonai, Tal. Newasa.

Dist, Ahmednagar. (M.S.)

14 membered tetraazamacrocyclic ligand have been synthesized and characterised by

UV- visible, FTIR, H1NMR spectra, mass, elemental analysis as well as biological activity.

The coordination chemistry of macrocyclic ligand containing a heteroatom are important

complexing agents or molecule. The macrocyclic ligand depends upon a number of factors

such as number and type of donor atoms present in the ligand. The macrocyclic ligand of

various β- diketone with O- phenilinediamine in ethanol are prepared by the template

condensation method have been already reported.

Keywords: Macrocyclic ligand complexes, FTIR spectra,H1NMR spectra etc.

OH

Cl

O O

O

+NH2 NH2

Reflux( 80 0C)

NN

N NOH

OH

Cl

Cl

O

O

HCl (pH = 3 ) / Ethanol




Green Chemistry R. V . Laware, G. P. Kadu

Padmashri Dr. V. V. P. Polytechnic, Pravaranagar , At Post : Loni, Taluka : Rahata, Dist :

Ahmednagar : 9881921043/ 7588605620

In the year of 1970 ―First Earth Day‖ was celebrated when EPA was formed. Under

EPA hundreds of laws were announced but majority of them were made to control pollution

after it is formed.Nations of the world taking challenge. They aggressively seek to stimulate

their economies, to create new jobs, to increase the accessibility of products and technologies

that enhance the quality of life. At the same time they desparately pursue the reversal of a

perceived global environmental crisis. This can be done in a way that provides a path to

environmentally sustainable development for all citizens of the planet.

Green Chemistry is design of chemical products and their processing in such a way as

not to produce any toxic or hazards substances. And if formation of toxic substances is

unavoidable, then their percentage should be reduced. As risk is a function of hazards and

exposure, In order to avoid this kind of pollution due to toxic substance as either product or

bi-product, two alternatives can be suggested. One way of thinking is to stop processes

producing toxic substances, which if implemented risk will be zero. Another way which

would work on this will be limit our exposure to such chemicals. The either case is difficult

to implement in growing sciences and technologies.To think over these issues, lot of research

work in academics as well as industries is going on. Several meetings and conferences are

held every year in all parts of the world.

The Presidential Green Chemistry Challenge Awards was announced by Clinton

administration in 1995. These awards are a means of recognizing outstanding achievements

in applied green chemistry and are the only awards in chemistry given out on the presidential

level. Nominees for these awards must demonstrate how their work has met one or more of

the following criteria:

• Better and greener reaction conditions for an old synthesis

• Better product synthesis using old chemicals.

• The synthesis of a new compound that is less toxic but has the same desirable

properties as an existing compound.

Key Words:

EPA : Environmental Protection Agency

VOC: Volatile Organic Compounds, DCM: Dichloromethane.



Development of Spectroscopic method for the determination of organic pollutants by

Green Analytical Techniques.

Vijay Bhusal, Kailas Tambe , Vitthal Vikhe

Pravara Rural Education society’s Sir Visvesvaraya Institute of Technology, A/P –Chincholi, Applied

Science Department,Tal- Sinnar,Dist-Nashik,M.S. India Pin-422101.

Spectroscopic measurements should have a well known techniques for the environmental

analysis because they allow for operational sim- plicity and low cost. In addition, methods

based on luminescence signals have high sensitivity. It is well known that the most

commonly employed analytical techniques for the determination of organic pollutants are

chro-matographic ones, including from the more simple methods to tandem-liquid

chromatography– mass spectrometry (TLC-MS) or gas chromatography-mass spectrometry

(GC–MS/MS). For samples of complex composition, intensive efforts must be done for

clean-up and fully resolving the chromatographic bands. The aim of this study was to reduce

the organic pollutant in the environments by Green Analytical Techniques (GAT). It‘s a

sustainable techniques as a practice of chemical science and manufacturing in a manner that

is sustainable ,safe and non pouting. That consumes minimum quantity of materials and

energy while producing less quantity of waste materials in the environments.

Keywords:- Silver Nano- Particles (SNP); Green Analytical Techniques (GAT); Tandem-

liquid chromatography– mass spectrometry (TLC-MS); Gas chromatography-mass

spectrometry (GC–MS/MS)

Solar Photocatalytic Degradation Of Rhodamine B Using Co-Doped Sno2 Supported On

Activated Carbon

S.R. Kande a

, U.G. Ghoshir a

, S.S. Jadhava, S.B. Pawar

b, G.G. Muley

c, A.B. Gambhire

b,*

a Research Centre, Department of Chemistry, New Arts, Commerce and Science College,

Ahmednagar, Maharashtra,, India bDepartment of Chemistry, Shri Anand College, Pathardi, Dist. Ahmednagar, 414102, Maharashtra,

IndiacDepartment of Physics, Sant Gadge Baba Amravati Univeristy, Amravati, 444602, Maharashtr,

*Corresponding author. Tel. +91 02428 222736, fax: +91 02428 223033;

E–mail address: [email protected] (A.B. Gambhire).

A series of SnO2/AC nanomaterials were prepared by doping V(III), Cr(III), Mn(II),

Fe(III), Co(III), Ni(II), Cu(II), Zn(II) with nitrogen and Sulphur, separatelyby co-

precipitation method, combined with surfactant incorporation method. The prepared sample

were characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM),

X-ray photoelectron spectra (XPS),Brunauer-Teller method (BET), UV – Vis diffuses

reflectance spectroscopy (DRS). The results shows that phase composition, crystallite size,



BET surface area, and optical absorption of samples varied significantly with the nature

dopants. The photocatalytic activities of these codoped SnO2 catalysts were investigated by

degradation Rhodamine B (RhB) in aqueous solution under solar-light illumination. The

results showed an appreciable enhancement in the photoactivity of the Zn, Scodoped

SnO2/AC as compared to other codoped SnO2/AC because of smaller particle size, higher

surface area and typical amphoteric behavior Zn and Snoxides in Zn, S codoped SnO2. The

degradation rate of Rhodamine B (RhB) reached 98% in 30 min which is about 5 times

higher than that of the standard P25 photocatalyst.

Keywords: Active carbon; co-dopant; Rhodamine B; Solar photocatalysis

Utilization of honey as sweetener for the preparation Of yoghurt drink

R.V. Darade , A. A.Walunj and P. B. Abhang College of Agriculture, Nagpur and College of Agriculture, Loni Ahmednagar

[email protected]

The present investigation was conducted with an objective tostudy the sensory and

chemical quality of honey based yoghurt . The yoghurt was prepared with different levels of

honey6,8,10, and 12 percent in treatment T2,T3, T4andT5 respectively. On the basis of

sensory evaluation the yoghurt prepared with 12 per cent honey T5 was found superior and

accepted extremely by the panel of judges. In respect of chemical composition totalsolid and

titratable acidity of yoghurt drink were increased with increase in level of honey. While fat,

protein and ash were decreased with increase in level of honey.The cost of production of 1

kgyoghurt drink was increased with increase in the level of honey. The lowest cost of

production (Rs. 46.58) was recorded in case of yoghurt drink prepared with addition of sugar

at 10 per cent (T1). However, the highest cost of production (Rs.87.48) of yoghurt drink with

12 per cent honey (T5) was found is the best treatment selected by panel of judges for sensory

evaluation.

Removal Of Heavy Metals From Aqueous Solution By Low Cost Adsorbent

Dr. Sureshkumar Halnor

Department of Chemistry

Doctor Vithalrao Vikhe Patil College of Engineering Ahmednagar (M.S.) India

email:[email protected]

Syzygium Cumini Leaf Powder (SCLP) was treated with nitric acid and used as a low cost,

easily available, natural adsorbent for the removal of heavy metal like chromium ions from

aqueous solution and waste water. Batch experiments were carried out to study effect of

temperature, adsorbent dose, initial concentration of adsorbate and pH. Adsorption capacity



was found to be enhanced by increasing adsorbent dose. Maximum adsorption was found to

be at pH 2. Equilibrium data were well represented by the Freundlich isotherm model for all

tested adsorption systems. This study showed that the Chromium adsorption phenomenon

onto SCLP was favorable and spontaneous.

H-NMP Catalysed Efficient Green Protocol for the Synthesis of Pyrazolopyridines

Anil G. Gadhave and Bhagwat K. Uphade

P. G. Department of Chemistry and Research Centre, Padmashri Vikhe Patil College,

Pravaranagar, Dist-Ahmednagar-413713, India.

[email protected]

Ionic liquid catalysed efficient synthesis of pyrazolopyridines by multicomponent reaction of

ethylacetoacetate, aldehyde and ammonium acetate is presented. The reaction works very

clean without formation of any side products. The present protocol offers several advantages

over reported methods such as short reaction time, good yields, easy workup and useful for

differently substituted aldehydes. The formation of products was confirmed by spectral

techniques and matching them with literature reported compounds.

Keywords: pyrazolopyridines, aldehydes, ethylacetoacetate, HNMP.

Scheme:

O

O

O

O

O

O

++

CHO

R

+ NH4OACHNMP

Ethanol

reflux NH

NNH

NNH

R

Removal of crystal violet dye from aqueous solution by calcinized eggshells

B. K. Uphadea &A. V. Borhade

b*

a Research Center, Department of Chemistry, P.V.P College, Pravaranagar, 413713, India

Email:[email protected]

b Research Center, Department of Chemistry, HPT Arts and RYK Science College, Nashik, 422005,

India, Email:[email protected]

The calcinized eggshells were obtained by waste eggshells materials and characterized by

FT-IR, XRD, SEM, EDAX, TGA and TEM analysis technique. The calcinized eggshells

have average crystallite size about 30 nm. The calcinized eggshells nanomaterials were used

for the removal of crystal violet dye. The removal of crystal violet dye from aqueous solution



at different concentration of dye, amount of photocatalyst, pH and nature of photocatalyst has

been studied. The maximum removal of crystal violet dye was observed at pH-7.6.

Keywords: Calcinized eggshells, FT-IR, XRD, SEM, EDAX, TGA and crystal violet dye.

[(Diacetoxyiodo) benzene] (DIB) catalyzed three component one pot synthesis of 1, 8-

Acridinedione derivatives under solvent free conditions

Amit S.Waghmare, Kailash R. Kadam

Post Graduate and Research Centre, Department of Chemistry, Padmashri Vikhe Patil College of

Arts, Science and Commerce, Pravaranagar, Ahmednagar 413713 (MS), India

E-mail:[email protected]

An efficient and convenient protocol is developed for the synthesis of 1, 8-acridinedione

derivatives by a one pot, three component condensations of dimedone, aldehydes and

ammonium acetate in the presence of catalytic amount of DIB at solvent free condition. The

present method provides several advantages such as high yields, shorter reaction times, easy

work-up procedure and purification of products by non-chromatographic methods has been

developed.

Keywords: Dimedone, DIB, acridinediones, solvent free, one pot synthesis

O

O

NH4OAcAr H

OO OAr

NH

214

+ +

3

DIB (10 mol%)

100 0C2

Scheme Synthesis of 1, 8-acridinedione derivatives

References

1. Heald R. A., Stevens M. F. Org. Biomol. Chem. 2003, 1, 3377-3389.

2. Girault S., Grellier P., Berecibar A., Maes L., Mouray E., Lemiere P., Debreu M. A.,

Davioud-Charvet E., Sergheraert C., J. Med. Chem., 2000, 43, 14, 2646-2654.

3. Sondhi S. M., Bhattacharjee G., Jameel R. K., Shukla R., Raghubir R., Lozach O., Meijer

L. Cent. Eur. J Chem. 2004, 2, 1-15

4. Michon V., Du Penhoat C. H., Tombret F., Gillardian J. M., Lepagez F., Berthon L., Eur.

J. Med. Chem., 1995, 30, 147-1



Synthesis and Characterization of Mn (II) & Ni (II) complexes of 4-chloro-2-[(E)-[1-(4-

fluorophenyl)-1H-pyrazol-4-yl](hydroxyimino)methyl]phenol

Namdeo T. Dhokale1, Bausaheb K. Karale

2 and Arvind V. Nagwade

1*

1Department of Chemistry, Ahmednagar College, Ahmednagar (MS)

2Department of Chemistry, Radhabai Kale Mahaila Mahavidyalaya, Ahmednagar, (MS)

Corresponding Author- [email protected]

Transition metal complexes having many applications in biological, clinical, analytical,

catalytic, microbial, insecticidal, antibiotic, plant growth regulator, food additive, tumor

inhibitor etc. All these applications keep in mind we synthesized Mn (II) & Ni (II) complexes

of 4-chloro-2-[(E)-[1-(4-fluorophenyl)-1H-pyrazol-4-yl](hydroxyimino)methyl]phenol by

dropwise addition of 2 equivalent of ligand in alcohol to a hot solution of 1 equivalent of

metal sulphate (MnSO4, and NiSO4) (Acidified with concentrated HCl) solution prepared in

distilled water. A slight excess of solution was added to metal solution. Then digest the

content on water bath. Then add alcoholic ammonia in above digested content the colored

complex precipitate out. Filter on suction pump and wash with little hot water and then

alcohol to remove excess of Ligand.

All the synthesized final compounds were first analyzed by performing TLC and melting

point determination. The percentage of metal was determined in laboratory by titration

method. Then the structure of metal complexes was confirmed by IR spectra. Furthermore,

biological activities of synthesized compounds ware screened against bacillus subtilis,

Staphylococcus aureus, actinomycetes and pseudomonas. The antibacterial data reveled that

ligand and their metal complexes show considerable antimicrobial activity.

Keywords: Pyrazole ligand, Metal Complex, Characterization, Antimicrobial.

Synthesis and biological evaluation for anti-depressant activities of triazole substituted

phenothiazine derivatives.

Magar Sagar D*, Dighe Amol S, Dighe Nachiket S.

Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Loni, MS, India-

413736. In this study, a series of triazole substituted phenothiazine derivatives was

synthesized and evaluated for their antidepressant activity by forced swim test (FST).The

synthesized compounds were tested for purity which was confirmed by melting point and TLC.

A structure of final compounds was confirmed by CHN analysis, IR and 1H-NMR.

Antidepressant activity of all the synthesized compounds was evaluated by despair swim test




by using Sprague-Dawley Rats. Standard drug Imipramine was used as the control. In the

despair swim test, all the synthesized derivatives showed antidepressant activity. Among them

four Compounds (A3, A9, and A13) showed significant antidepressant activity comparing with

control drug imipramine. These results are useful for the further investigation in the future.

Keywords: Antidepressant activities, Despair swim test, Phenothiazine and Sprague Dawley

Rats.

Studies on Total Lipid Content of Some Wild Edible Fruits using Conventional and

Ultrasound Method

Vaishali J. Khilari†, Pramod P. Sharma

† and Somnath S. Gholap

††

†Research Centre in Botany, Shri Muktanand College, Gangapur, 431109(MS), India

††

Department of Chemistry and research Center, Padmashri Vikhe Patil College of Arts, Science and

commerce, Pravaranager (Loni kd), Tal: Rahata, Dist: Ahmednager, Maharashtra, Pin: 413713,

India.

E-mail:[email protected];[email protected]

The present communication deals with the investigation of lipid contents of some wild fruits

of Diospyros melanoxylon, Pithecellobium dulce, Carrisa congesta, Lantena camera, opuntia

and Aegle marmelis. The total crude lipid determination was conducted using conventional a

nd ultrasound assisted extraction technique using methanol, methanol-chloroform and petrole

um ether as a solvent. It has been observed that ultrasound assisted extraction is superior in te

rms of time (1-2 hr) and yield of the crude lipids. As there are no detailed work done on lipid

profile of nutritious wild fruits as such. Present study focused on the food value and explorati

on of underutilized edible fruits in Maharashtra, India.

References

1. Fayez Hamam Food and Nutrition Sciences, 2013; 4:63-70.

2. Hausman DB, Higbee DR, and Grossman BM, ―Dietary Fats and Obesity,‖ Akoh CC, an

d Min DB, Eds., Food Lipids, Marcel Dekker, Inc., New York, 2002:663-694.

3. Ma YQ, Chen JC, Liu DH, Ye XQ, Ultrasonics Sonochemistry, 2009; 16: 57-62.

4. Salar BD, Mortazavi SA, Rezaei K, Rajaei A, Karimkhani MM, Food Science and Biotec

hnology, 2012; 21(4):1005-1011.

5. Ahmed M, Akter MS, Eun JB, (2011). International Journal of Food Sciences and Nutriti

on, 2011; 62(1): 91-96.

6. Teng H, Jo IH, & Choi YH, Journal of the Korean Society for Applied Biological Chemist

ry, 2010; 53(5):618-625.

7. Zou TB, Jia Q, Li HW, Wang CX, & Wu HF, Marine Drugs, 2013; 11:1644-1655.



Synthesis And Biological Evaluation Of Some Novel 2, 5 –Disubstituted [1, 3, 4]-

Oxadiazole Bearing 2, 2-Dimethyl-2, 3-Dihydrobenzofuran Scaffold As Potential Anti-

Tubercular Agents

Macchindra S. Tambea, Laxman Nawale

b, Dhiman D. Sarkar

b, Somnath S. Gholap

a,*

aPost graduate Department and research centre, Padmashri Vikhe Patil College, Pravaranagar, A/P

Loni kd, Tal.- Rahata, Dist.- Ahmadnagar, Pin 413713 (MS), India. bOrganic Chemistry Division, Combichem-Bioresource Centre, National Chemical Laboratory, Pune

– 411008, India. *Corresponding author- E-mail : [email protected]; Tel : +912422273426

A series of novel 2,5-disubstitued 1,3,4-oxadiazole derivatives bearing 2,2-dimethyl-2,3-

dihydrobenzofuran scaffold has been synthesized and were evaluated for antitubercular

activity. The synthesized compounds were characterized by IR, 1H- NMR,

13C-NMR and

Mass spectral study. The antitubercular data for this series suggested that growth inhibition

MTB and BCG can be imparted by the introduction of a hydroxybut-1-ynyl group at 4-

position of 12j. Most compounds were more active against non-replicating than replicating

cultures of Mycobacterium tuberculosis H37Ra by ex vivo as well as by in vitro and

Mycobacterium bovis BCG an unusual pattern with respect to existing anti-TB agents. Three

of the compounds showed MIC in the range of 2.31–23.91 µg /mL proving their potential

activity. The active compounds were studied for cytotoxicity against three cell lines and were

found to be non-cytotoxic. Specificity of these compounds was checked by screening them

for their anti-bacterial activity against four bacterial strains (Gram-negative strains: E.coli,

S.aureus; Gram-positive strains: P.aeruginosa and B.subtilis).

O

NN

OF3CHN

O

OHO

NN

OF3CHN

O

R

12: MIC = 2.31-23.91 µg /mL GI50 <90; >55 µg/mL

M. tuberculosis H37Ra

(ATCC 25177) IC90 = 2.31 ±0.69 µg /mL (vivo)

M. tuberculosis H37Ra

(ATCC 25177) IC90 = 2.91 ±0.89 µg /mL (vitro)

12j :

M. Bovis BCG (ATCC 35743) IC90 = 2.77 ±0.92 µg /mL (vitro)

References

1. Russell D. G.; Barry C. E.; Flynn J.E. (2010) Tuberculosis: what we don't know can,

and does, hurt us. Science, 328, 852-856.

2. Dye C.; Williams B.G. (2010) The population dynamics and control of tuberculosis.

Science, 328, 856-861.




3. Ma Z.; Ginsberg A. M.; Spigelman M. (2007) Global alliance for TB Drug

Development, New York, NY, USA, 7, 699-730.

4. Young D. B.; Perkins M. D.; Duncan K.; Barry C.E. (2008) Confronting the scientific

obstacles to global control of tuberculosis. Clin. Invest. 118, 1255-1265.

Evaluation Isolation and Characterization of Chemical constituents from C. bonducella

L. seed

SunayanaVikhe*, Sunil Nirmal.

Department of Pharmacognosy, Pravara Rural College Of Pharmacy, Loni M.S. India-

413736.

The objective of the present work is to study the different Pharmacognostic parameters of the

seeds of C. bonducella and to isolate and characterize the chemical constituents from the

seeds that are responsible for the activity.In pharmacognostic study of seeds of C. bonducella,

macroscopy, microscopy, powder characteristic, and physical parameters were

studied.Column chromatography of active extract; Structure elucidation of active fraction

was done to isolate and characterize various chemical constituents.The alcohol soluble

extractive value was found to be greater than water soluble extractive value. Petroleum ether

extract showed the presence of steroids and terpenes. Ethanol extract showed positive test for

flavonoids, alkaloids, glycosides, and tannins. By GC-MS analysis of saponified matter of

petroleum ether extract contains fatty acid viz. hexadecanoic acid and 9-methyl-8-tridecen-2-

ol, acetate. The unsaponified matter contains colour pigments namely lycoxanthin and

carotene.

Keywords- Caesalpiniabonducella, seeds, extract, Thin layer Chromatography, GC-MS.

Effect of L-Ascorbic Acid Supplementation on Dicofol Induced Alteration in the

Ascorbic Acid Levels of an Experimental Model Parreysia cylindrica

R. S. Tambe Department of Zoology,

Arts, Commerce and Science College, Satral,Tal: Rahuri, Dist: Ahemadnagar (MS), India.

[email protected]

An investigation was undertaken to evaluate the effectiveness of ascorbic acid on Dicofol

induced alteration on the ascorbic acid levels in an experimental model, the fresh water

bivalve Parreysia cylindrica. The effect on bivalve was studied under five groups. Group A

was maintained as control, group B bivalves were exposed to chronic dose of ( LC 50/10

values of 96 hrs) of dicofol (0.04023 PPM) up to 21 days while group C bivalve where

exposed to respective chronic concentration of Dicofol with 50mg/L of L- ascorbic acid for

21 days. Were divided into D and E group. The D group bivalves were allowed to cure



naturally while E group bivalves were exposed to 50mg/L of L-ascorbic acid for recovery.

After every seven days bivalves from A, B, C, D & E were removed, and their tissue were

separated and dried at 80 0C. From the dried powders, total proteins were estimated by

Lowry‘s method (1951) . From each powder, ascorbic acid level was estimated and presented

as mg/gm of dry weight. Significant decrease was observed in ascorbic acid content on the

exposure to dicofol. However; depletion in the L-ascorbic acid level on the exposure to

dicofol with L-ascorbic acid was minimum. Pre-exposed bivalves to dicofol showed fast

recovery in the ascorbic acid exposed bivalves than those, which were allowed to recover

naturally; the probable role of L- Ascorbic acid is discussed in the paper.

Key words: Ascorbic acid, dicofol, Parreysia cylindrica.

Removal of crystal violet dye from aqueous solution by calcinized eggshells

Development and Validation of HPTLC Method for Estimation of Gymnemic Acid in

Microencapsulated AntidiabeticPolyherbal Formulations

Nirmal S.A, Vikhe D.N and Dukre T.P

Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni.

E-mail: [email protected]

Gymnemic acid (GA) is one of the phytoconstituents present in Gymnemasylvestre.

Estimation of GA was carried out first time from microencapsulated polyherbal formulation.

Microencapsulated polyherbal formulations (F1 and F2) contain various plant extracts; hence,

proper resolution of GA peak in high-performance thin-layer liquid chromatography

(HPTLC) analysis of F1 and F2 is the problem. Hence, HPTLC analysis method for F1 and

F2 is developed and validated for quantitative determination of GA. HPTLC analysis of F1

and F2 was carried out using TLC aluminium plates precoated with silica gel 60F254 eluted

with chloroform–methanol–water (6.5 mL + 4.5 mL + 1.0 mL), and densitometric analysis

was carried out at 580 nm. Complete validation was performed using standard methods. This

HPTLC method was found to be reproducible, accurate, and can detect GA at microgram

level. The new optimized mobile phase gave good resolution of GA peak for its proper

quantification in microencapsulated polyherbal formulation.Gymnemic acid extracted from

polyherbal formulation F1 and F2 capsuleshowed single spots at RF = 0.64 ± 0.01. The % of

gymnemicacid from capsule was found to be 98.16% (F1), being 109.44% (F2), beingwell

within the limits.




Exploitation of microbes to enhance crop productivity- a sustainable approach

Durgude R. A. and V. S. Supe

Department of Horticulture, MPKV., Rahuri-413722. Maharashtra.

Increasing population and high demand for food security putting a vast amount of pressure on

agriculture sector to enhance crop productivity. Attempts to increase productivity should be

accompanied with sustainable approach. In spite of using more and more chemicals,

exploitation of microbes for increasing productivity will surely fulfill both purposes. Here we

are focusing on microbes such as plant growth promoting rhizobacterias (PGPRs) that are

exploited for increasing productivity of various crops. The PGPRs increase crop productivity

by synthesizing phytohormones including auxins, cytokinins and gibberellins, producing

siderophores which can solublize and sequester iron, affect nutrient availability by secreting

organic acids to solubilize nutrients, atmospheric nitrogen fixation, modifying rhizospheric

soil environment by polysaccharide production, activating plant defense mechanism against

biotic stresses, colonizing roots etc. The intentional inclusion of such bacteria in soil

ecosystem is proved beneficial in increasing crop productivity thus ensuring food security

along with sustainability.

“Aqueous Medium One Pot Synthesis of 2-subsituted Benzimidazole”

Amruta K. Mhaske, Rani J. Gaikwad, Vinod R. Kadu

Department of Chemistry, ASC College, Kolhar

Benzimidazole structures are classified under several classes of drugs based on the

possible substitution at different position of the benzimidazole nucleus. Introduction of a

small substitution into the 2 &5 position is characteristic for benzimidazole anti-helmenties;

alternatively, bulky 2-substituent characterize drugs used in the peptic ulcer and are

sometimes revered as proton pump inhibition; bulky 1&2 subsituents are found in HI-anti-

helmentics. All these compounds contain the benzimidazole skeleton and hence it has been

assumed that this skeleton is necessary for the therapeutic effect.

One pot synthesis of benzimidazoles requires the heating of o-phynylendiamine

(ODP) and carboxylic acids. Acid and its derivatives as nitrile, chloride, ortho estere etc can

be used for above said synthesis. Reaction requires strong acid condition and sometimes high

temperature and even sometimes use of polyphosphoric acid or by microwave irradiation.

Other methods reported for the preparation of benzimidazole in the literature.these includes

of OPD with α β unsaturated carbonyl compounds, β-haloketones or ketones in the presence

of BF3-EtO, NaBH4 ,polyphosphoric acid and MgO and POCl3. Many of these methods suffer



from one or other limitations such as requiring harsh reaction conditions, low to moderate

yields long reaction times, tedious work of procedures co-occurance of several side products.

Keywords: drugs, anti-helmenties, microwave irradiation.

Biosynthesis, Characterization and antimicrobial activities of Nickel Nanoparticles

Using Ocimum sanctum (Tulsi) Leaf Extract

S. R. Kuchekar1, H. R. Aher

1 and P. M. Dighe

2

1Analytical Chemistry Laboratory, Department of Chemistry,

2 Department of Physics P. V. P. College, Pravaranagar, At/Po. Loni(Kd), Tal. Rahata, Dist.

Ahmednagar

Nickel nanoparticles (NPs) are gaining importance for their uses inVarious fields. In the

present investigation NiNPs were synthesized by green route using Ocimum sanctum (Tulsi)

leaf extract. NiNPs were generated by reduction of Nickel sulphate (NiSO4) solution with

plant extract. The reductants present in the plant extract acts as an reducing and stabilizing

agent. The resulting Nickel nanoparticles were characterized by UV-VIS spectrophotometry,

IR, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive

Spectroscopy (EDX). These nanoparticles shows antibacterial property towards pseudomonas

and E-coli.

DABCO: An efficient and green catalyst for one pot four-component synthesis of 11-

amino-12-(4-aryl)-8,9,10,12-tetrahydro-7H-chromeno[2,3-b]quinolin-3-ol derivatives.

Akash D. Gholap and Shivaji S. Pandit*.

Research Centre and Post Graduate Department of Chemistry, Padmashri Vikhe Patil College of

Arts, Science and Commerce Pravaranagar At./Po. Loni kd. Tal.Rahata. Dist. Ahmednagar. 413713.

(MS). India. (Affiliated to Savitribai Phule Pune University, Pune, India)

A series of 11-amino-12-(4-aryl)-8,9,10,12-tetrahydro-7H-chromeno[2,3-b]quinolinol

derivatives were synthesized through one-pot four-component reaction of resorcinol,

malononitrile, aromatic aldehydes and cyclohexanone in presence of DABCO as an

inexpensive catalyst under solvent-free condition using stone grinding techniques at room

temperature. The advantages of this method are the use of an inexpensive and green catalyst,

short reaction time, and easy workup with good to excellent product yields.



Studies on Carbohydrate and Protein Contents of Some Underutilized Wild Fruits

Vaishali J. Khilari†, Pramod P. Sharma

† and Somnath S. Gholap

††

†Research Centre in Botany, Shri Muktanand College, Gangapur, 431109(MS), India

††

Department of Chemistry and research Center, Padmashri Vikhe Patil College of Arts,

Science and commerce, Pravaranager (Loni kd), Tal: Rahata, Dist: Ahmednager,

Maharashtra, Pin: 413713, India.

E-mail:[email protected];[email protected]

Western Ghat (Maharashtra), India is well known for excellent plant and animal biodiversity.

Due to presence of abundant wild edible fruit species, native tribal peoples were regularly co

nsuming these fruits or fulfilling food necessity. Eleven wild and underutilized edible fruits of

Diospyros melanoxylon Roxb., Pithecellobium dulce Roxb., Carissa congesta Weight., Lanta

na camara L, opuntia stricta (Haw.) Haw., Aegle marmelos L, Terminalia catappa L, Zizyphu

s mauritiana Lam, Limonia acidessima L., Elaeagnus conferta Roxb. and Anacardium occide

ntale L. have high nutritional potential and medicinal properties. The present study deals with

the investigation of carbohydrate and protein content of these eleven wild edible fruits using

standard protocols. The carbohydrate and protein content was compared with some commonl

y consumable fruits. The exploitation of wild these fruit plants are the main intentions of the

present study fruits as nutritious food for wild as well as urban citizens and to deliver the info

rmation about commercial value.

References

1. Sharma P. P. and Savant R. J. 2012. Some less-known plants parts as supplementary food

s International Multidisciplinary Research Journal, 2(12), 12-13.

2. Sharma P. P., Mulay J. R. 2014. Some Underutilised Plant Resources as a source of food f

rom Ahmednagar District, Maharashtra, India. Discovery, 9(23), 58-64.

OH

+

O

CN

CN

CHO

+ +

HO

DABCO

(5 mol %)

Solvent free

Stone Grind

rt

HO O N

NH2

R

R

Scheme1: One pot four component synthesis of chromeno[2,3- b]quinolin-3-ol derivatives.

1 2 3 4 5



3. Sharma P. P., Vijigiri D., Bembrekar S. K. 2013. Wild Tribal Food Plants of Adilabad Di

strict, Andhra Pradesh, India, International Journal of Pharmaceutical and Biological Scie

nces Fundamentals, 3(1), 30-34.

4. Sharma P.P. and Khilari Vaishali J. 2016. Studies on Ascorbic acid content of some wild

edible fruits from Shendi and Ratanwadi, Maharashtra, International Journal of Advanced

Research, 4(5), 583-590.

5. Sharma P.P. and Khilari Vaishali J. 2016. Studies on Total Lipid Content of Some Wild E

dible Fruits using Ultrasound, Journal of Advanced Scientific Research 7(2), 20-24.

Synthesis and biological Screening of some Halogenated Chromones.

aVijay A. Kadnor,

aGajanan R. Pandhare, Sharad N. Shelke

b

aP.G. Department of Chemistry, Arts, Commerce and Science College, Satral Tal-Rahuri, Dist.

Ahmednagar 413711.(M.S) b Department of Chemistry, S.S.G.M. College, Kopargaon, Dist-Ahmednagar (MH) 423601, India.

Synthesis of flavones and their derivatives have considerably attention invited

everybody‘s due to their significant biocidal, pharmaceutical, antioxidant, anti-anxiolytic,

anti-cancer and anti-inflammatory effects. Chromones have broad spectrum of applications in

the field of synthetic chemistry, pharmacological and physiological processes.

In present investigation we had done the synthesis of some halogenated chromones.

In first step we got the Carbazole chalcones which have been synthesized by well known

Claisen-Schimidt condensation of aldehyde with various substituted O-hydroxy

acetophenone. These Chalcones which upon cyclilisation by using DMSO/I2.afford the

targeted compounds. Synthesized compounds were characterized by IR, mass and 1HNMR

spectral techniques and these compounds were tasted for biological activity.

“Green Catalyst for effective Organic Synthesis”

Vinod R. Kadu*, Amol K. Kharde

*, Somnath S. Gholap

1

*Assistant professor, Department of Chemistry, ASC College, Kolhar

1Assistant professor, Department of Chemistry, PVP College, Pravaranagar

Green Chemistry is indeed one of the innovative creativity and discovery of Chemistry. The

need was generated by fact that we had little understanding of adverse effects of chemicals on

human health and environment. This field will allow chemist to play their important role in

designing a safer, healthier and more sustainable world. Just less than two centuries ago

organic compounds were believed to be accessible through biological process. Greener path



for synthesis of organic compounds are now available. One such includes use of micelle

generating surfactants as catalyst. Aqueous phase of such surfactants can be used as catalyst.

Synthesis of diazole derivatives from aldehyde, hydrazine and terminal alkynes can be

effectively catalyzed by such catalyst.

R1

CHO+

R2

HN

NH2+

10% Aq. Solution of Suractant

Room Temperature Stirring, 4 Hrs

NNR2

R3

R1R3

Aldehyde Hydrazine Terminal Alkynes Diazole

Scheme

Keywords: human health, environment, sustainable, surfactants, catalyst

Antitubercular activity of Thiophene and Thiazole anchored flavones

B K Karalea & S J Takate*

b

aDepartment of Chemistry, Radhabai Kale Mahila Mahavidyalya, Ahmednagar, 414001, India

bDepartment of Chemistry, New Arts, Commerce and Science College, Ahmednagar, 414001, India

E-mail: [email protected]

Tuberculosis is one of the contagious diseases which is associated with high mortality

worldwide. In search of potential antitubercular agents various heterocycles have been

investigated. In continuation to previous antimicrobial studies of thiophene, thiazole

containing flavones, the compounds were studied against Mycobacterium tuberculosis H37Ra

and Mycobacterium bovis BCG using Rifampcin as reference compound. All the test

compounds exhibited weak antitubercular activity.

O

O

S

N

S

CH3CH3

R



Effect Of Aluminium On Human Health

Smt. Kanchan Samish Deshmukh Womens College of Home Science & Bca Loni (kd), Rahata, Dist-Ahmednagar

[email protected]

Aluminum is the most abundant metal in the earth‘s crust. However, it is not essential

for life. Concern about Al toxicity to humans, including from food sources, has persisted

since the demonstration that it has the potential to be a neurotoxicant (Wiley 1928, 1929;

Schaeffer et al. 1928; Döllken 1898; Gies 1911; Anon. 1913; Yokel and Golub 1997; WHO

1997; Krewski et al. 2007; ATSDR 2008). Most of the aluminium in human body get through

food, water, beverages and medicine, passes through the digestive system without being

absorbed by the body. High levels of aluminium in the body have been shown to have

neurotoxic effects, effects on bone and possibly reproduction. This occurs when the amount

of aluminium consumed exceeds the body‘s capacity to excrete it, because the aluminium is

then deposited in our bodies and can cause problems. Foods and beverages are the single

largest contributor of Al intake for the typical human, providing 3.5 to 10 mg/day. Food

additives provide a significant percentage of the daily intake. Among the food additives,

sodium aluminum phosphates (SALPs) are the main contributors. The wide use of aluminium

cookware and storage vessels, the intake of aluminium by Indian population is much higher

than what has been reported for the West. In this paper, we review the effects of aluminum on

human health. Considering its long half-life in the body, unnecessary exposure to aluminum

should be avoided for human health.

‟ Save Me From Global Warming”

C. S. Karle Department of Chemistry, Arts, Commerce and Science , Satral,Tal: Rahuri,

Dist: Ahemadnagar (MS), India. [email protected]

The chemical industry releases many ingredients that in the wrong place are harmful

to the environment, human health or both. Six types of environmental pollutions land

pollution, water pollution, air pollution, thermal pollution, light pollution and noise pollution.

Earth absorbs some of the radiant energy received from the sun reflects some of it as light

and reflects or radiates the rest back to space as heat. Earth's surface temperature depends on

this balance between incoming and outgoing energy. If this energy balance is shifted, Earth's

surface could become warmer or cooler, leading to a variety of changes in global climate.

Global warming causes by greenhouse effect. Greenhouse gases in the atmosphere act like a

mirror and reflect back to the Earth a part of the heat radiation, which would otherwise be lost




to space. Three main greenhouse gases are carbon dioxide, methane and nitrous oxide.

Greenhouse gases rise up into the atmosphere and envelop the Earth; the gases that play a

role in global warming The higher the concentration of green house gases like carbon dioxide

in the atmosphere causes warming of the earth and the sea. A number of natural and man-

made mechanisms can affect the global energy balance and force changes in Earth's climate.

Global warming is due to emission of carbon dioxide. . 72% of the totally emitted greenhouse

gases is carbon dioxide (CO2), 18% Methane and 9% Nitrous oxide (NOx). Carbon dioxide

emissions therefore are the most important cause of global warming. The reflecting back of

heat energy by the atmosphere is called the "greenhouse effect". Burning diesel fuel.

Burning fuel is called an oxidation process. Consider C12H26 is formula for diesel fuel. The

chemical reaction during burning 2 C12H22 + 37 O2 → 26 H2O + 24 CO2. Each kg of diesel

fuel burnt, 1.38 kg water vapor and 3.106 kg CO2 is produced. Man made activity increasing

the amount of non-water vapor greenhouse gases in the atmosphere by burning fissile fuels,

cattle breeding, etc. This causes the temperature to rise. An increased temperature causes

more water to water to evaporate into the atmosphere, which does in turn increase the

warming effect. Chlorofluorocarbon is also green house gas. It is due ozone-depleting. The

ozone layer is located in the stratosphere, a region of the atmosphere that is about 10 to 50

kilometers above the Earth. Individuals can prevent ozone depletion by avoiding products

that contain chlorofluorocarbons, or CFCs, hydro fluorocarbons, or HCFCs. The function of

the ozone layer is to shield the Earth from the harmful ultraviolet rays of the sun. Without the

ozone layer, the sun's ultraviolet radiation would negatively affect life on land and in the

water, leading to mass extinction. Effects of global warming are increase of temperature on

the earth, rise of sea levels due to thermal expansion of the ocean, in addition to melting of

land ice. Greenhouse gases can be removed from the atmosphere by various processes, the

CO2, is reduced by photosynthesis of plants, and dissolving in the oceans, reacts to form

carbonic acid and bicarbonate and carbonations. Use green solvents, less toxic chemicals, use

catalysts etc. Also avoid products containing chlorofluorocarbon. Use renewable energy

sources reduce percentage of carbon dioxide and save the earth from global warming.

Keywords: Green, global, green gases, carbon dioxide.



Soil Health Card for Sustainable Crop Production

Dr. A. G. Durgude Dept. of Soil Science and Agril. Chemistry, MPKV., Rahuri.

[email protected]

The Government of India has launched a massive flagship scheme of generating Soil

Health Cards (SHC) by testing soil samples using GPS based one grid sample per 2.5 ha. of

irrigated land and 10 ha. of rainfed land across India for soil type, pH, EC, CaCO3, available

N, P, K, organic C, secondary nutrients (S, Mg and Ca) and micronutrients (Zn, Mn, Fe and

Cu) and issuing them to 140 million farmers within a period of 3 years. The SHC will also

contain crop-wise fertilizer recommendations on the basis of soil analysis. As far as

Maharashtra is concerned, a target of 1,39,32,049 SHCs has been fixed to be issued to the

farmers.

Challenges of soil health

Maintenance of soil health is the key to sustainable high productivity, good water and

air quality. The term soil quality and soil health are often used interchangeable in the

scientific literature. Soil health is defined as continued capacity of soil to function as a vital

living system, within ecosystem and land use boundaries to sustain biological productivity,

maintain the quality of air and water environment. Soil health encompasses not only crop

productivity and environmental protection but also food safety, and animal/human health.

India needs to produce at least 350 million tons of food grains to feed the projected

population of 1.41 billion by 2025. India produced about 218.7 million tons of food grains

during 2009-10. The yields of the crops grown in dryland areas remained very low and the

overall partial factor productivity of fertilizers to added fertilizers is declining year after year.

The partial factor productivity of fertilizers decreased from 42 kg grain/kg NPK applied in

1975 to 18 in 1985 to 13 in 1995 and 8 in 2010. The decline the rate of response of crops to

added fertilizers under intensive cropping systems has possibly resulted from deterioration in

physical, chemical and biological health of soils. Depletion of soil health in terms of soil

organic carbon and available plant nutrients from dryland areas are some of the major threats

to the soil productivity and land degradation. To meet the requirement of growing demand of

food grains it is imperative to increase the production potential through improvement of soil

health in rainfed and dryland regions besides the irrigated regions. Important issues related

soil health degradation are listed below

Physical degradation such as compaction, crusting etc. by excessive cultivation

through mechanization.

Chemical degradation of soils: the major reasons are



Wide nutrient gap between demand and supply.

High nutrient turnover in soil-plant system coupled with low and imbalanced

fertilizer use.

Emerging deficiencies of secondary and micronutrients

Poor nutrient use efficiency

Insufficient inputs of organic sources

Salinity and alkalinity in soils

Irrigation induced water logging

Biological degradation by organic matter depletion and loss of soil fauna and flora

Soil pollution from industrial waste, excessive use of pesticides and heavy metal

contamination.

What is soil health Card ?

The soil health card studies and reviews the health of soil or rather we can say a

complete evaluation of the quality of soil right from its functional characteristics, to water

and nutrient‘s content and other biological properties. It will also contain corrective measures

that a farmer should adopt to obtain a better yield.

How does it help the farmers?

With the issue of the card, the farmer will get a well- monitored report of the soil

which is chosen for cultivation of crops

The monitoring will be done on a regular basis

The farmers will be guided by experts to come up with solutions to improve the

quality of the soil

Regular monitoring will help the farmers to get a long-term soil health record which

will helpful for improving of problem soils by adopting different organic or inorganic

amendments.

This card can become most helpful and effective when filled out regularly by the

same person over a period of time.

The idea is not to compare the varied soil types but to find out methods to improve

soil fertility, to access the different types of soil and their ability to support crop

production in spite of their limitations and as per their abilities.

The soil card will help the farmers to get an idea on the crop wise recommendation of

nutrients and fertilizers required in each type of soil. Therefore, soil health card can

help farmers for getting sustainable crop yield and useful for maintaining soil health.



Thus, the SHC can be the visa or mantra for transforming Indian agriculture in

general and that of Maharashtra in particular into truly an agribusiness model by

integrating the Central Governments schemes like the Make in India, the Skill India,

Pradhan Mantri Sinchai Yojana etc This will insulate our farmers from distress and

promote robust livelihood option in a sustainable manner.

A review on Applications of Nanotechnology

A.P. Londhe*, B.R. Mhaske

Department of Engineering Science, Pravara Rural engineering College Loni

A/P- Loni Dist-Ahmednagar 413736

Nanotechnology is one of the most promising scientific field today since it combines

knowledge from the fields of Physics, Chemistry, Biology, Medicine, Informatics, and

Engineering. It is an advanced technology with large applications in various fields with great

potential which can be applied in real life. The advanced nano materials, nano devices and

biomaterials are fabricated and controlled by different nanotechnologies and tools, which

investigate and tune the properties, responses, and functions of living and non-living matter,

at sizes below 100 nm. The application and use of nanomaterial in electronic and mechanical

devices, in optical and magnetic components, quantum computing, tissue engineering, and

other biotechnologies, with smallest features, widths well below 100 nm, are the

economically most important parts of the nanotechnology nowadays andin future also.

The number of nanoproducts is rapidly growing since more and more nanoengineered

materials are reaching the global market The continuous revolution in nanotechnology will

result in the fabrication of nanomaterials with properties and functionalities which are going

to have positive changes in the lives of our citizens, be it in health, environment, electronics

or any other field. In the energy generation challenge where the conventional fuel resources

cannot remain the dominant energy source, taking into account the increasing consumption

demand and the CO2 emissions alternative renewable energy sources based on new

technologies have to be promoted. Innovative solar cell technologies that utilize

nanostructured materials and composite systems such as organic photovoltaic offer great

technological potential due to their attractive properties such as the potential of large-scale

and low-cost roll-to-roll manufacturing processes. The advances in nanomaterial necessitate

parallel progress of the nanometrology tools and techniques to characterize and manipulate

nanostructures. Revolutionary new approaches in nanometrology will be required in the near

future and the existing ones will have to be improved in terms of better resolution and



sensitivity for elements and molecular species. Finally, the development of specific guidance

for the safety evaluation of nanotechnology products is strongly recommended.

Keywords: Nanotechnology, nanostructures, nano devices, applications.

Green Chemistry in Textile Industry

Ms. Anuradha Gajanan Wandhekar

Womens College of Home Science & Bca Loni (kd), Rahata, Dist-Ahmednagar

[email protected]

Conventional chemical processes based on fewer fuels are unsustainable. Green reactions are

sustainable, more efficient (fewer steps, fewer resources, less waste), easier to use (stable

under ambient conditions), eco-friendly (non-hazardous solvents and less hazardous waste).

They are assessed by twelve principles, the most important being the amount of waste

generated. The textile industry is considered as the most ecologically harmful industry in the

world. Recently a number of steps have been taken to make textile processing greener. These

include use of greener fibre, greener dyes and auxiliaries, greener solvents, eco-friendly,

optimised and efficient processing, bio-processing, recycling of textile, water and chemicals

and elimination of hazardous chemicals.

Synthesis and study on optical properties of Nd doped calcium lanthanum borate

glasses

Gajanan B. Hardea, Gajanan G. Muley

b,*

aDepartment of Physics, Shri R. R. Lahoti Science college, Morshi, Maharashtra, India-444905

bDepartment of Physics, Sant Gadge Baba Amravati University, Amravati, Maharashtra, India-444602

*Corresponding author. Tel.: +91 721 2662279, ext-269; fax: +91 721 2660949, 2662135.

E-mail address: [email protected] (G.G. Muley)

Nd doped calcium lanthanum borate (Nd:CLB) glasses have been prepared by melt

quenching method. Glass phase of the material has been confirmed by powder X-ray

diffraction technique. Optical properties have been studied by recording ultraviolet-visible-

near infra red transmission spectra. Optical band gap of the glass has been evaluated. The

absorption peaks corresponding to the Nd transitions levels have been evaluated. The single

beam z-scan method has also been used for measurement of nonlinear (NL) refractive index

(n2) and NL absorption coefficient (β).

Keywords: Nd: CLB glass; melt quenching method, NL refractive index, absorption

coefficients.




Study on effect of Nickel Doping on Optical Properties of L-Arginine Phosphate

Crystals

Gajanan G. Muley1,*

, Anil B. Naik2, Anil B. Gambhire

3, Deepak T. Tayade

4

1Department of Physics, Sant Gadge Baba Amravati University, Amravati, Maharashtra, India-

2Department of Chemical Technology, Sant Gadge Baba Amravati University, Amravati,

Maharashtra, India-444602 3Department of Chemistry, Shri Anand College, Pathardi, Dist. Ahamdnagar

4Department of Chemistry, Government Vidarbh Institute of Science and Humanities, Amravati


E-mail address: [email protected] (Dr. G.G. Muley)

In present investigation crystals L-arginine phosphate (LAP) crystals doped with nickel

chloride (NiCl2) material have been grown from the aqueous solution at room temperature by

slow evaporation technique. The crystalline phases of grown crystals have been confirmed by

powder X-ray diffraction technique. Optical properties have been studied by recording

ultraviolet-visible-near infrared transmission spectra. In case of NiCl2 doping, the

transparency and the band gaps of the pure and doped crystals has been calculated and found

to be decrease with doping concentration. Photoluminescence (PL) study confirm the

presence of dopants levels as it show absorption in the range 300-500nm.

Keywords LAP crystals; slow evaporation technique; powder X-ray diffraction technique;

ultraviolet-visible-near infrared spectroscopy.

Development and Progress in Fiber Optic Urea Biosensor

Sunil N. Botewad, Vikas G. Pahurkar, Gajanan G. Muley*

Department of Physics, Sant Gadge Baba Amravati University, Amravati-444602, Maharashtra,

India

*Corresponding author. Ph.D.; Tel.: +91 9850325379. E-mail address: [email protected]

(Dr. G.G. Muley).

Technical improvement and progress in biosensor field has been increased due to its

application in various fields. The impact of biosensor is more over conventional sensing

techniques because of their specificity, small size, ease of handling, high precision and ability

for real-time as well as on-spot analysis. The different types of bio-species such as glucose,

urea, cholesterol etc. are precisely measured by biosensor in earlier literature. The present

work has been reported one of the bio-species i.e. urea. The different types of transducers,

immobilization techniques and immobilization matrix found for fabrication of urea biosensor

elsewhere. Besides of this idea the immobilization techniques and immobilization matrix



used for development of urea biosensor using optical fiber transducer has been discussed

briefly.

Keywords: biosensor; optical fiber; urea; urease; immobilization

Single Crystal Growth of Mg2Na2ZnB4O10 (MNZB) and Nonlinear optical study

S. R. Dagdale and G. G. Muley

Department of Physics, Sant Gadge Baba Amravati University, Amravati, Maharashtra, India-444602


E-mail address: [email protected] (Dr. G.G. Muley)

The synthesis of Mg2Na2ZnB4O10 (MNZB) crystal was synthesized system via solid-state

reaction method. The MNZB crystal grown was slow cooling method. The obtained

polycrystalline borate materials MNZB were investigated by energy dispersive X-ray analysis

(EDXA), scanning electron microscope (SEM). EDXA analysis is carried out to find the

existence of basic elements of MNZB. SEM analysis was carried out to analyse the surface

morphology. The results are presented and discussed. Second harmonic generation has been

witnessed.

Keywords: MNZB crystal; slow cooling method; EDXA; SEM;

Optical Fiber based Sensor for Ammonia detection: A Review

Vishal D. Wankhade1, Gajanan B. Harde

2, Vikas G. Pahurkar

1, Gajanan G. Muley

1*

1Department of Physics, Sant Gadge Baba Amravati University, Amravati-444602, Maharashtra, 2Department of Physics, Shri R. R. Lahoti Science college, Morshi-444905, Maharashtra,India

*Corresponding author. Ph.D.; Tel.: +91 9850325379. E-mail address: [email protected]

Many research articles have been published in last few years regarding toward the gas sensors

to sense various hazards gases such as ammonia, carbon dioxide, ethylene, diethyl ether,

methane etc. Present review has been reported the sensor system and basic concepts as well

as mechanism for ammonia gas sensing. Moreover it highlights the recent research

development in ammonia gas sensor using U-bend and surface plasmon resonance (SPR)

fiber optic sensor. As well as, the application of ammonia sensor in environmental

monitoring, medical gas analysis and agriculture gas analysis have been reviewed shortly.

The advantages over other traditional sensor also has been discussed in detail.

Keywords: fiber optics sensor; ammonia sensor; optical sensor




Biochemical Changes Due To Application Of Bioagent’s Innaculants Used For Biological

Management Of Root-Knot Nematode, Meloidogyne Incognita Infesting Pomegranate

Mr. S. N.Varpe*, Dr. A. R. Walunj, Dr. N. L. Mhase

*College of Agriculture, Loni Tal. Rahata Department of Entomology, Mahatama Phule Krishi

Vidypeeth,Rahuri-413 722( Maharashtra state), India

The investigations on effectiveness of different bioagents against root-knot nematode

infesting pomegranate saplings were carried out with biochemical changes in the

pomegranate sapling roots due to application of bioagents were studied in Biochemistry

laboratory under Forage Research Project, M.P.K.V., Rahuri, during 2014-15.The

effectiveness of different bioagents against root-knot nematode infesting pomegranate

saplings was studied with eight treatments including untreated control.

The biochemical changes in roots of pomegranate saplings treated with different

bioagents were studied and it was observed that the activity of peroxidase and polyphenol

oxidase enzymes and per cent total polyphenols were more up to 30 days in saplings treated

with nematicide, carbofuran 3 G. Whereas, the activity of peroxidase and polyphenol oxidase

enzymes and total polyphenols was found to be more at 30, 60, 90 and 120 days after

treatments in the roots of saplings treated with bioagent, Phule Trichoderma plus than the

other treatments. More the activity of peroxidase and polyphenol oxidase enzymes and total

polyphenols in the roots of pomegranate saplings showed the lower number of root-knot

nematode population, root galls and egg masses in pomegranate saplings.

Evaluation of promising genotypes of ber (Zizyphus mauritiana Lamark) against its pest

Complex.

Miss. R.E. Papade and Dr. A. R. Walunj

Department of Entomology, Mahatama Phule Krishi Vidypeeth, Rahuri-413 722( Maharashtra state),

The investigation on evaluation of promising genotypes of ber against its pest‘s complex

were carried out at AICRP, Arid Fruit Zone Project, Department of Horticulture, MPKV,

Rahuri, Dist. Ahmednagar, Maharashtra during 2015-2016.

Among the twenty genotypes Chandegaon Sel., and Chalisgaon were found

significantly superior in registering least damage due to leaf eating caterpillar, leaf webber

and fruit damage due to fruit borer and stone weevil, which could be used as best source of

resistance in future. For the management of fruit borer and stone weevil on ber, two sprays

spinosad 2.5 SC, fipronil 5 EC @ 1.0 ml and Neem oil + Pongomia oil @ 2.0 ml / lit at 50



percent flowering and fruiting stage can be considered better for the control of the pests and

gave better marketable yield of ber.

Compatibility Of Insecticides With Fungicide And Fertilizers On Pomegrnate

Dr. Ashok R. Walunj* and Rahul Lad

Scientist *(Entomology) ,Arid Zone Fruit Department of Horticulture, Mahatma Phule Krishi

Vidyapeeth, Rahuri-413 722( Maharashtra state), India

Pesticides and fertilizers are important input in pomegranate fruit production. To save the

costs, time and expenditure farmers are sometimes apply both of them in combination for

gaining the more benefit. During the preparation of mixture if compatibility is not considered

there may be adverse affect on plant which may cause damage to plants.This could be

achieved by knowing the nature of each chemical compound ,mode of action, efficacy,

compatibility and judicious application. Some times pesticides sprayed with fertilizer

enhance the efficiency of pesticide. Therefore, present investigation was carried out to study

the compatibility of cyantraniliprole is a second-generation anthranilic diamide insecticide

insecticide mixed with fungicide and soluble fertilizers at AICRP, AZF, Department of

Horticulture. MPKV. Rahuri. Present findings indicated that the the combination of

cyantraniliprole 10.26 %OD @ 0.2 ml + Carbendazim 50WP @ 1.0 g + Soluble fertilizer

0:52:34 @5 g per litre water found most compatible without affecting any physiochemical

properties, phytotoxicity and biological efficiency against pests (viz.aphids, thrips and fruit

borer) on pomegranate .

Rapid Determination of tellurium(IV) by Ultraviolet Spectrophotometry using o-

methylphenyl thiourea as a new chromogenic ligand

Shashikant R. Kuchekara, Shivaji D. Pulate

b

aDepartment of Chemistry and research Center, Padmashri Vikhe Patil College of Arts, Science and

commerce, Pravaranager (Loni kd), Tal: Rahata, Dist: Ahmednager, Maharashtra, Pin: 413713, bAnalytical Chemistry Laboratory, Department of Chemistry, Arts, Commerce and Science College,

Satral, At/Po. Satral, Tal. Rahuri, Dist. Ahmednagar, MS, India, 413711

The objective of this research work was to develop a simple, highly sensitive and

precise method for spectrophotometric determination of tellurium(IV). O-Methylphenyl

thiourea (OMPT) coordinates with tellurium(IV) as a 1:1 (tellurium(IV)-OMPT) complex in

hydrochloric acid media (7.0 mol l-1

). The novelty of investigated method is instant complex

formation at room temperature with no need of heating or standing. Method is applicable

over wide Beer‘s range (up to 70 µg ml-1

). A low reagent concentration is required (2 ml,

0.01 mol l-1

in ethanol). The complex exhibits maximum absorption in the wavelength 280



nm and was selected for further study. The molar absorptivity was 1.98 × 104

L mol-1

cm-1

,

Sandell‘s sensitivity was 0.00641 µg of tellurium(IV) cm-2

. Proposed method was

successfully applied for analysis of real sample.

Keywords: Tellurium(IV); UV-spectrophotometry; Analysis, real sample.

Knot Nematode, Meloidogyne Incognita Infesting Pomegranate

Mr. S.N.Varpe*, Dr. A. R. Walunj and Dr. N. L. Mhase

College of Agriculture,* Loni Tal. Rahata

Department of Entomology, Mahatama Phule Krishi Vidypeeth,

Rahuri-413 722( Maharashtra state), India

The investigations on effectiveness of different bioagents against root-knot nematode

infesting pomegranate saplings were carried out with biochemical changes in the

pomegranate sapling roots due to application of bioagents were studied in Biochemistry

laboratory under Forage Research Project, M.P.K.V., Rahuri, during 2014-15.The

effectiveness of different bioagents against root-knot nematode infesting pomegranate

saplings was studied with eight treatments including untreated control.

The biochemical changes in roots of pomegranate saplings treated with different

bioagents were studied and it was observed that the activity of peroxidase and polyphenol

oxidase enzymes and per cent total polyphenols were more up to 30 days in saplings treated

with nematicide, carbofuran 3 G. Whereas, the activity of peroxidase and polyphenol oxidase

enzymes and total polyphenols was found to be more at 30, 60, 90 and 120 days after

treatments in the roots of saplings treated with bioagent, Phule Trichoderma plus than the

other treatments. More the activity of peroxidase and polyphenol oxidase enzymes and total

polyphenols in the roots of pomegranate saplings showed the lower number of root-knot

nematode population, root galls and egg masses in pomegranate saplings.

Future perspective for formaldehyde pathways for reductive synthesis and energy

storage.

Tambe Digambar C, Kothule Revannath A, Wabale Jaydip D.

PG Department of Chemistry, Department of Chemistry, Arts, Commerce and Science College,

Satral, At/Po. Satral, Tal. Rahuri, Dist. Ahmednagar, MS, India, 413711

Formaldehyde is a key platform reagents in the chemical industry for many decades in a large

number of bulk scale industrial processes. Thus the annual global demand reached 30

megatonnes. Per years and currently it is solely produced under oxidative, energy intensive

conditions, using high temperature approaches for the methanol oxidation in a recent



years,new field of application beyond the use of formaldehyde and its derivatives as i.e,

synthetic reagent or disinfectant have been suggested. For eg. dialkoxy metane could be

envisioned as direct fuel for combustion engines or aq. Formaldehyde and Para

formaldehydes may acts as liquid organic hydrogen generation to be used for hydrogen fuel

cells.To turn these new perspective in feasible approaches,it requires also new less energy

intensive technologies for the synthesis of formaldehyde. This perspective spreads light on

the recent direction towards the low temperature reductive synthesis of formaldehyde and its

derivative and low temperature formaldehydes reforming for hydrogen generation. This

aspects are important for the future demands on modern societies renewable energy

management inform of a methanol and hydrogen economy and the required formaldehyde

feedstock for the manufacture of many formaldehyde base dairy product.

Keywords-Formaldehyde, hydrogen fuel cells,hydrogen economy.

Green Chemistry A Natural Evaluation Initiative For Prevention Of Environment

Pollutions

Prashant L. Harale, Dilip S. Aute, Ms.D.D.Agarkar Department Of Chemistry, Arts, Commerce and Science College Satral, Tal.Rahuri,

Dist.Ahmednagar, MS

Green Chemistry is the new way of reduction environment pollutions by designs safer

Chemicals and processes. Need of green chemistry in day to day life is to prevent man made

pollutions. Green Chemistry helpful for reduction and generation of environmental hazardous

chemicals at primary stage by using environment friendly chemicals and processes.

Implementation of Green Chemistry by adapting its principals in today‘s life is important

initiative for healthy environment. There are number of eco-friendly chemicals and processes

which helpful for creating sustainable environment.

Key word: Green Chemistry, Environment, Sustainable

“Green Alternatives for Organic Synthesis”

Kavita K. Raut, Amol K. Kharde, Vinod R. Kadu

Department of Chemistry, Arts Commerce and Science College, Kolhar

Adverse effect on environment such as toxicity or damage to the stratosphere ozone

layer is the result of industrial chemical reactions which include organic solvents such as

chlorofluorocarbons, benzene, and carbon tetrachloride.

The use of Supercritical carbon dioxide (SC-CO2), as a reaction medium for

heterogeneous selective oxidation using conventional and "green catalysts" and for



hydrogenation of organic compounds that have pharmaceutical applications. It is an energy

conserving, selective and waste reducing alternative to organic solvents. Selectivity and

efficiency, easy of manipulation, and more importantly, because toxic and often volatile

solvents are avoided in solvent-free conditions remarks the advantageous of such reactions.

Solvent-free approaches involve grinding and mixing, microwave (MW) irradiation of neat

reactants, or catalysis by the surfaces of inexpensive and recyclable mineral supports, such as

alumina, silica, clay, or ‗doped‘ surfaces. Development and deployment of products

containing nanomaterials is now a worldwide phenomenon. The unique physical and

chemical properties of nanomaterials, such as different conductivity, optical sensitivity, and

reactivity, originate mainly from factors such as small size, surface structure, chemical

composition, shape, solubility, or aggregation. The design and synthesis of green oxidants for

use as alternatives to traditional oxidants can offer an environmental solution to the area of

oxidation catalysis. Selective oxidation catalysis has been identified as one of the most

environmentally hazardous industrial processes.

Keywords: toxicity, ozone layer, green catalysts, microwave, nanomaterials.

“Synthesis of Bio-diesel from Vegetable oil”

Rani J. Gaikwad, Amruta K. Mhaske

Assistant professor, Department of Chemistry, ASC College, Kolhar

Biodiesel is an alternative diesel fuel that is produced from vegetable oils & animal fats. It

consists of the mono alkyl esters formed by a catalyzed reaction of the triglycerides in the oil

or fat with a simple monohydric alcohol. The reaction conditions generally involve a trade-

off between reaction time and temperature as reaction completeness is the most critical fuel

quality parameter. Much of the process complexity originates from contaminants in the

feedstock, such as water and free fatty acids, or impurities in the final product, such as

methanol, free glycerol, .Processes have been developed to produce biodiesel from high free

fatty acid feedstock‘s, such as vegetable oil ,animal fats.

Keywords- Bio- Diesel, Trans-esterification ,methanolysis.



Green Chemistry: Tool for Healthy Environment

Dubey Anuradha* .Wandhekar Meenakshi**

Women’s College of Home Science And BCA,Loni

[email protected]

Environmental issues such as air; water and land pollution, climatic changes, and

renewable energy have become important issues in our daily life. Many people think that

chemistry as well as chemical industries are harmful to the environment. However, nowadays

many new advances and scientific researches in the field of chemistry developed to event

more environment friendly applications and objects, while they held keep up with the

lifestyle we expect. An example of environmental friendly chemistry is green chemistry.

Green chemistry is the use of chemistry for pollution prevention and control..The term Green

chemistry, coined in 1991is ―the design of chemical products and processes that reduce or

eliminate the use and generation of hazardous substances‖ This approach to the protection of

human health and the environment represents a significant departure from traditional methods

previously used. In this paper applications of green chemistry principals to prevent

Environmental pollution are highlighted

Key Words: Green chemistry, pollution, environment.

Applications of Green Chemistry Principles in every Day life Rohamare S.S.Dighe S.G.,Palghadmal V.S.

Department of Chemistry, Arts, Commerce And Science College , Satral

Green chemistry is the new and rapid emerging branch of chemistry. The beginning of green

chemistry is considered as a response to the need to reduce the damage of the environment

by man-made. Materials and the processes used to produce them. Green chemistry could

include some basic principle. It is to prevent waste than to treat waste after it has been

created. Synthetic methods should be designed to maximize the incorporation of all materials

used in the process into the final product. Synthetic methods should be use and generate

substances that possess little or no toxicity to human health and the environment. Chemical

products should be designed to affect their desired function while minimizing toxicity. The

use of auxiliary substances should be made unnecessary wherever possible. Energy

requirements of chemical processes should be recognized for their environmental condition.

A raw material should be renewable rather than depleting whenever technically and

practicable. Unnecessary derivatives should be avoided. then Catalytic reagents are used.

Chemical products should be designed at the end of their function they break down into

innocuous degradation products and do not persist in the environment. Analytical



methodologies need to be further developed to allow for real-time, in process monitoring and

control prior to the formation of hazardous substances. Substances used in a chemical

process should be chosen to minimize the potential for chemical accidents,including releases,

explosions and fires. All the principle are shows the basic idea of green chemistry in

protecting the environment from pollution and atom economy,toxicity, solvent , application

of raw materials from renewable sources ,degradation of chemical products to

simple,nontoxic substances. Principles of green chemistry can contribute to sustainable

development.

Keywords: Green chemistry, Environment, Sustainability.

A study on phyto-chemical Screening and antibacterial activity of Gymnema sylvestre

against pathogenic strains

Ranjit R. Raut1, Dnyaneshwar M. Shimbre

2 Ashok M. Bhosale*

3 &

Anil R. Kurhe*

4

1Department of Zoology, New Arts, Commerce and Science College, Ahmednagar.

2Department of Zoology, Rao Bahadur Narayanrao Borawake College, Shrirampur Department of

Botany and Department of Zoology Arts, Commerce and Science College, Satral, (Savitribai Phule

Pune University, Pune) M.S. India.

Corresponding Email: [email protected]

Abstract Gymnema sylvestre is medicinal plant belongs to the family ―Asclepladaceae‖. Gymnema

sylvestre contains an organic acid called ―Gymnemic acid‖. The study shows it is useful in

controlling blood sugar to treat diabetes. Present study involves the antibacterial activity and

phytochemical screening of the hexane, chloroform and methanol extracts of leaves of Gymnema

sylvestre. The antibacterial activity was evaluated by agar well diffusion method against four

Gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus

vulgaris) and five Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Micrococcus

luteus, Staphylococcus aureus, Streptococcus pneumonia).Methanol extract showed good

antibacterial activity with the high inhibition zones, while chloroform extract exhibited mild to

moderate activity and hexane extract was found to be less active. Phytochemical screening

revealed the presence of various secondary metabolites like steroids, alkaloids, phenols,

flavonoids, coumarins, saponins, tannins and triterpenoids. The results of the present study

suggest that leaves of Gymnema sylvestre can be used to treating infectious diseases caused by E.

coli and S. aureus. Gymnemagenin was obtained after acidic hydrolysis followed by basic

hydrolysis of the sample and extraction into ethyl acetate.

Keywords: Gymnema sylvestre, Antimicrobial Activity, Phytochemical Analysis, Methanolic

Extracts, Gymnemagenin.




obtained after acidic hydrolysis followed by basic hydrolysis of the sample and extraction

into ethyl acetate.

Keywords: Gymnema sylvestre, Antimicrobial Activity, Phytochemical Analysis, Methanolic

Extracts, Gymnemagenin.

Know and Practice Nature’s Protocols for Sustaining Future of Humanity: Biocatalysis

Dr. R. A. Mane Department of Chemistry

Dr. Babasheb Ambedkar Marathwada

University, Aurangabad - 431004

Email: [email protected]

Mother nature is masterful chemist and performs various biotransformations instantly

at ambient temperature. World‘s population is increasing and hence there is huge demand of

varied valuable materials for compliance of daily needs for retaining healthy life and

sustaining humanity. Various manmade activities, carried for achieving these goals are found

to be burdensome to environment, as most of the processescontribute topollute air and water

quality andexploite non-renewable natural resources. To overcome these lacunas and keeping

society healthy with sustainable humanity, there is an urgent need to understand nature‘s

protocols ofbiotransformations and practice them with responsibility while performing

chemical transformations, required for generating the necessaryvalue added materials

namely; drugs, healthcare products, smart materials, polymers etc.

Keeping this in view and considering the current recommended thrust areas here in

the plenary lecture attention will be paid on biocatalysisfor organic transformations and also

sharing our contribution to the field, used for generating rapidly library of various

heterocycles of therapeutic/clinical significance. The optimization details of various

cyclocondensations/ condensations along with the role exhibited by various enzymes in their

rate acceleration will be debated.




Silver Nanoparticles Sytnhesized under Clinorotation and Assesment of their Cyto-

Genotoxicity

Avinash J. Aher@†

and Pandit B. Vidyasagar†

†Department of Physics, University of Pune, Pune-411007, India.

@ Presently at University Hospital Erlangen, Friedrich Alexander University,

Erlangen, Germany.

From past several years microgravity has been used as a tool in field of biological and

physical processes. Microgravity environment provieds a unique window to gain a better

understanding of how gravity driven phenomena like sedimentation, bouyancy driven

convection, solidification and crystal growth get affected. Microgravity allows researchers to

study underlying events free from these effects. Materials science research in microgravity

can lead to a better understanding of how materials are formed and how the properties of

material are influenced by their formation in microgravity. In this regard, present study deals

with studying the effects of clinorotation (simulated microgravity) on biosynthesis of silver

nanoparticles using bacterium Escherichia coli (DH5α). To simulate microgravity condition,

1-dimensional horizontal axis clinostat has been used. This 1-d clinostat was designed and

developed in our laboratory to simulate microgravity condition, where rpm can be selected by

adjusting voltage of the DC motor. Rotation speed of clinostat was kept at 2 rpm which

resulted in an acceleration force of ~7x10-5

g. Silver nanoparticles synthesized under

simulated microgravity condition were characterized by means of UV-Vis spectroscopy,

Electron diffraction spectroscopy (EDX), Transmission Electron Microscopy (TEM) and X-

ray Diffraction (XRD). These AgNPs were further screened for their cyto-genotoxic activity

against cervical cancer cell lines and human skin keratinocytes (HaCaT) using alkaline comet

assay. Results indicated that AgNPs caused dose dependent reactive oxygen species

production that leads to DNA damage and decreases cell viability.



Production of enzymes by Bradyrhizobiumjaponicum strains

Kalpana Palghadmal, A. M. Bhosale

Department of Botany Arts, Commerce, and Science College Satral,

Tal .Rahuri, Dis. Ahmednagar, M. S.

Email: [email protected]

Ten strains of Bradyrhizobiumjaponicum where isolated from ten localities from

Pravara area and labeled as B1, B2, B3, B4, B5 ………. B10 these isolates were tested for

enzyme production. It was interesting to note that all the isolates were capable of producing

polygalacturase enzyme. Polygalacturonase activity was higher in B4 strain ( 925.92 RVU)

followed by B3 strain (649.35 RVU).

The C1 cellulase activity was higher in B8 (155.03 RVU) followed by B3

(95.23RVU),Cxcellulase activity was higher in B1 (0.326 Moles/ml/min.) followed by B2 (

0.319 Moles/ml/min.), amylase activity was the highest in B1 (0.731 Moles/ml/min.)

followed by B2 (0.708 Moles/ml/min.).These results indicate that the production of different

enzymes varies with different strains but all the strains were capable to produce these

enzymes. This supports that with the help of these enzymesB. japonicum penetrate in to root

hair.

Keywords – Bradyrhizobiumjaponicum ,Polygalacturase, C1cellulase, Cxcellulose,Amylase

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