ICoFAB2021 - techno2.msu.ac.th

ICoFAB2021 International Conference on Food, Agriculture and Biotechnology

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Preface

The Faculty of Technology, Mahasarakham University (MSU), Thailand initiated the 1st International Postgraduate Symposium on Food, Agriculture and Biotechnology in 2014 (IPSFAB 2014) with the main aims to provide a stage for Thai and international postgraduates to present their research at the international stage. In 2018, the symposium had been renamed as ‘International Conference on Food, Agriculture and Biotechnology (ICoFAB)’ to include postgraduates, researchers and lecturers as primary participants and this has continued so till this day.

Our conferences in the past seven years (2014 – 2020) have been

successful and continued to grow in terms of turn outs and partnerships with

international institutes. We have been honored by distinguished scientific

committees who kindly have contributed to our Proceedings continually,

audiences and renowed keynote/invited speakers from the fields of Food,

Agriculture and Biotechnology from around the world. Every year, the potential

academic networks or research collaborations amongst Thai and international researchers have been developed.

Due to the unprecedented coronavirus pandemic around the globe in 2020, our conference has been transformed to the virtual online format since

ICoFAB2020 to encourage continuous sharing of research knowledge and close

academic networking in spite of social physical distancing.

This project was financially supported by Mahasarakham University. We

hope that the 8th ICoFAB2021 virtual conference would bring you fruitful

discussions and future research collaborative networks amongst national and international researchers while you can stay safe at home or at workplace during this difficult time.

(Asst. Prof. Dr. Sumonwan Chumchuere)

Dean of the Faculty of Technology

Mahasarakham University


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Organizing committee

1. Asst. Prof. Dr. Sumonwan Chumchuere

2. Dr. Sunisa Roidoung

3. Assoc. Prof. Dr. Maratree Plainsirichai

4. Assoc. Prof. Prasit Chutichudech

5. Assoc. Prof. Dr. Anut Chantiratikul

6. Assoc. Prof. Dr. Luchai Butkhup

7. Asst. Prof. Dr. Wantana Sinsiri

8. Asst. Prof. Dr. Sirirat Deeseenthum

9. Asst. Prof. Dr. Pheeraya Chottanom

10. Asst. Prof. Dr. Kedsirin Sakwiwatkul

11. Asst. Prof. Dr. Eakapol Wangkahart

12. Asst. Prof. Dr. Ruchuon Wanna

13. Asst. Prof. Dr. Chanyut Thamwan

14. Asst. Prof. Dr. Vijitra Luang-In

15. Mrs. Busaba Tharasena

16. Dr. Wanida Chuenta

17. Dr. Apichaya Bunyatratchata

18. Dr. Thitiwut Vongkampang

Scientific Committee

1. Prof. Hanny Wijaya, Institute Pertanian Bogor University, Indonesia

2. Prof. Yongqi Shao, Zhejiang University, China

3. Prof. Wu Xin, Chinese Academy of Sciences, Tianjin, China

4. Honorary Prof. Colin Wrigley, University of Queensland, Australia

5. Prof. Yueqiu H.E., Yunnan Agricultural University, China

6. Prof. Antonio J. Barroga, Central Luzon State University, Philippines

7. Prof. Emer, Chiba University, Japan


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8. Assoc. Prof. Dr. Yuthana Phimolsiripol, Chiang Mai University,

Thailand

9. Assoc. Prof. Dr. Chalor Jarusutthirak, Kasetsart University, Thailand

10. Assoc. Prof. Dr. Nyuk Ling Ma, Universiti Malaysia Terengganu,

Malaysia

11. Assoc. Prof. Dr. Khamsah Suryati Mohd, Universiti Sultan Zainal

Abidin, Malaysia

12. Asst. Prof. Dr. Rossaporn Jiamjariyatam, Srinakharinwirot University,

Thailand

13. Asst. Prof. Dr. Ekapol Limpongsa, Rangsit University, Thailand

14. Asst. Prof. Dr. Teerasak Punvichai, Prince of Songkla University,

Thailand

15. Asst. Prof. Dr. Po-Tsang Lee, National Taiwan Ocean University,

Taiwan

16. Asst. Prof. Dr. Jareeya Yimrattanabovorn, Suranaree University of

Technology, Thailand

17. Asst. Prof. Dr. Pongsak Khunrae, King Mongkut's University of

Technology Thonburi, Thailand

18. Dr. S. Krishnakumari, Kongunadu Arts and Science College, India

19. Dr. K. Surekha, Kongunadu Arts and Science College, India

20. Dr. Supaporn Chunchom, Rajamangala University of Technology Isan,

Thailand

21. Dr. Siriwan Panprivech, Assumption University, Thailand

22. Dr. Chukwan Techakanon, Prince of Songkla University, Surat Thani

campus, Thailand

23. Dr. Cao Thi Thanh Loan, Nong Lam University, Vietnam

24. Dr. Song Xiaoming, Hangzhou Normal University, China

25. Dr. Manoch Kongchum, Louisiana State University Agricultural

Center, USA

26. Dr. Ahmed Attaya, University of Massachusetts, USA


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27. Dr. Sohye Yoon, University of Queensland, Australia

28. Dr. Ottavia Benedicenti, University of New Mexico, USA


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Program of ICoFAB2021

DAY 1: Thu 5th August 2021

MC: Dr. Apichaya Bunyatratchata

Venue: Webex Webinar, live from TA201 room, Faculty of Technology,

Mahasarakham University, Thailand.

Time Activities

08.45 – 09.00 Registration

09.00 – 09.15 Report remark by Dean, Faculty of Technology, MSU

Welcome speech by MSU President

09.15 – 9.55 Keynote talk 1 ‘Thai curcuma species as functional foods’

by Prof. Dr. Jaehong Han, Chung-Ang University, Korea

9.55 – 10.25 Invited talk 1 on ‘Parameter estimation for modeling of

dynamic food systems’

by Prof. Dr. Kirk Dolan, Michigan State University, USA

10.25 – 10.40 Break

10.40 – 11.25 Oral presentations session 1

Chair: Prof. Dr. Sirithon Siriamornpun

10.40 – 10.55 1_ FN-O-001 Pharmacognostic specification and quality

control of Cannabis sativa L. subsp. sativa

Lukman Sueree

10.55 – 11.10 2_ FN-O-002 The study of LPG drying process for

sliced pork using mathematical modeling

Sanchai Yotmanee

11.10 – 11.25 3_FN-O-003 Development of novel pH sensitive

oxygen indicator based on Gallic acid for food safety

and security

Konala Akhila


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11.25 – 13.00 Lunch

13.00 – 13.40 Keynote talk 2 on ‘Migration phenomenon in food

packaging: friend of foe?’

by Asst. Prof. Dr. Hayati Samsudin, School of Industrial

Technology, Universiti Sains Malaysia, Malaysia

13.40 – 14.10 Invited talk 2 on ‘The role of active packaging in clean

labels foods’

by Asst. Prof. Dr. Kirtiraj K. Gaikwad, Indian Institute of

Technology Roorkee, India

14.10 – 14.25 Break


Chair: Dr. Apichaya Bunyatratchata

14.25 – 14.40 4_ FN-O-004 Development of bioplastic films into

functional packaging for food products

Nathdanai Harnkarnsujarit

14.40 – 14.55 5_ FN-O-005 Functional packaging of meat products from

nisin and EDTA incorporated films

Nathdanai Harnkarnsujarit

14.55 – 16.00 Poster presentation session

Poster will be displayed on the screen and can be seen at

https://techno2.msu.ac.th/icofab2021/

END OF DAY 1


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DAY 2: Fri 6th August 2021

MC: Asst. Prof. Dr. Pariyaporn Itsaranuwat

Venue: Webex Webinar, live from TA201 room, Faculty of Technology,

Mahasarakham University, Thailand.

Time Activities

08.45 – 09.00 Registration

09.00 – 09.40 Keynote talk 3 on ‘Design of pollutants screening methods in

water using photosynthetic microbes’

by Prof. Dr. Ling Shing Wong, INTI International University,

Malaysia

09.40 – 10.10 Invited talk 3 on ‘Breeding novelty Phalaenopsis orchids to

deploy for future market’

by Prof. Dr. Fure Chyi Chen, National Pingtung University

of Science & Technology, Taiwan

10.10 – 10.25 Break


Chair: Asst. Prof. Dr. Pariyaporn Itsaranuwat

10.25 – 10.40 6_BT-O-001 Riceberry broken rice and soybean meal as

substrates for exopolysaccharide production by Bacillus

tequilensis PS21

Thipphiya Karirat

10.40 – 10.55 7_ BT-O-002 Optimization of Arthrospira platensis growth

using organic culture medium

Aporn Bualuang

10.55 – 11.10 8_PT-O-004 Bio-water hyacinth (Eichhornia crassipes)

Fermented extracts supplemented plantation pots promote

growth of water spinach (Ipomoea aquatica)

Bongkoj Boonburapong


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11.10 – 11.25 9_ PT-O-005 Effects of bio-extracts from cyanobacteria

Nostoc sp. on growth and quality of Brahmi (Bacopa

monniera)

Surasak Laloknam

11.25 – 13.00 Lunch

13.00 – 13.40 Keynote talk 4 on ‘Immune response and protective efficacy

of a polymeric adjuvant (Montanide™ Gel 02 PR),

administered with a Streptococcus agalactiae ghost vaccine

in Nile tilapia (Oreochromis niloticus)’

by Mr. Regis Vialle, SEPPIC, France

13.40 – 14.10 Invited talk 4 on ‘Smart rice duck pig farming system: key to

precision agriculture’

by Prof. Dr. Antonio Barroga, Central Luzon State

University, Philippines


Chair: Asst. Prof. Dr. Ekapol Wangkahart

14.10 – 14.25 10_ PT-O-001 Effects of probiotic on corn silage quality

and rumen digestibility

Chanya Plumejai

14.25 – 14.40 11_ PT-O-002 Identification and transcriptional analysis of

the metal tolerance protein (MTP) gene family in cassava

under zinc deficiency

Natlita Payap

14.40 – 14.55 12_ PT-O-003 Effect of electromagnetic field on root

cassava cv. Rayong 72

Taweesab Chaiyarak

14.55 – 15.05 Closing remarks

THE END


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Contents

Page

Preface: 1

Organizing committee 2

Scientific committee 2

Program of ICoFAB2021 5

Abstracts:

Keynote talks:

Keynote talk 1 Thai curcuma species as functional foods

Prof. Dr. Jaehong Han

16

Keynote talk 2 Migration phenomenon in food packaging:

friend of foe?

17

Asst. Prof. Dr. Hayati Samsudin

Keynote talk 3 Design of pollutants screening methods in

water using photosynthetic microbes

18

Prof. Dr. Ling Shing Wong

Keynote talk 4 Immune response and protective efficacy of a

polymeric adjuvant (Montanide™ Gel 02 PR),

administered with a Streptococcus agalactiae

ghost vaccine in Nile tilapia (Oreochromis

niloticus)

19

Mr. Regis Vialle

Invited talks:

Invited talk 1

Parameter estimation for modeling of dynamic

food systems

Prof. Dr. Kirk Dolan

22


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Contents

Abstracts: Page

Invited talks:

Invited talk 2 The role of active packaging in clean labels

foods

Asst. Prof. Dr. Kirtiraj K. Gaikwad

23

Invited talk 3 Breeding novelty Phalaenopsis orchids to

deploy for future market

Prof. Dr. Fure Chyi Chen

24

Invited talk 4 Smart rice duck pig farming system: key to

precision agriculture

25

Prof. Dr. Antonio Barroga

Oral presentations:

1_FN-O-001

Pharmacognostic specification and quality control of

Cannabis sativa L. subsp. sativa

Lukman Sueree

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2_FN-O-002 The study of LPG drying process for sliced pork using

mathematical modeling

Sanchai Yotmanee

28

3_FN-O-003

Development of novel polyphenolic compound

coated pH based as oxygen indicators for food safety

and security

29

Konala Akhila

4_FN-O-004

Development of bioplastic films into functional

packaging for food products

30

Assoc. Prof. Dr. Nathdanai Harnkarnsujarit


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Contents

Abstracts: Page

Oral presentations:

5_FN-O-005 Functional packaging of meat products from nisin and

EDTA incorporated films

Assoc. Prof. Dr. Nathdanai Harnkarnsujarit

31

6_BT-O-001

Riceberry broken rice and soybean meal as substrates

for exopolysaccharide production by Bacillus

tequilensis PS21

32

Thipphiya Karirat

7_ BT-O-002

Optimization of Arthrospira platensis growth using

organic culture medium

33

Dr. Aporn Bualuang

8_ PT-O-004 Bio-water hyacinth (Eichhornia crassipes)

Fermented extracts supplemented plantation pots

promote growth of water spinach (Ipomoea aquatica)

34

Bongkoj Boonburapong

9_ PT-O-005

Effects of bio-extracts from cyanobacteria Nostoc sp.

on growth and quality of Brahmi (Bacopa monniera)

Surasak Laloknam

35

10_ PT-O-001

Effects of probiotic on corn silage quality and rumen

digestibility

36

Chanya Plumejai

11_ PT-O-002

Identification and transcriptional analysis of the

metal tolerance protein (MTP) gene family in cassava

under zinc deficiency

Natlita Payap

37


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Contents

Abstracts: Page

Oral presentations:

12_ PT-O-003

Effect of electromagnetic field on root cassava cv.

Rayong 72

Taweesab Chaiyarak

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Poster presentations:

FN-P-001 Physical alteration of the peeled durian stored in

“Ozone Box” odor lock packaging

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Dr. Siriwan Tungsangprateep

FN-P-002 Phytochemical, antioxidant activity and color

parameter from seed extract of Thai black rice in

different seasons

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Asst. Prof. Dr. Muntana Nakornriab

FN-P-003 Development of active film using agricultural by-

products: pomelo peel and karanda pomace

42

Dr. Sunisa Roidoung

PT-P-001 The pretreatment condition for chromosome count

and karyotype analysis of Dimocarpus longan from

Thailand

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Panurat Pipatchananan

PT-P-002 Insecticidal fumigant and repellent activities of seeds

essential oils from carrot (Daucus carota L.) against

maize weevil (Sitophilus zeamais Motschulsky)

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Asst. Prof. Dr. Ruchuon Wanna


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Contents

Abstracts: Page

Poster presentations: PT-P-003 The effect of nutrients deficiencies on Cannabis

sativa L. leaves

Natphatsorn Phiwatkunwarut

46

PT-P-004 Effect of rates and application methods of zinc on

growth, yield and economic return of cassava variety

Kasetsart50 cultivated in a loamy sand soil

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Asst. Prof. Dr. Benjapon Kunlanit

PT-P-005 Effects of cannabis lines on nutritional value and

bioactive ingredient compounds cannabis leaves

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Yolrawee Tongsak

PT-P-006 Chemical composition and bioactivity of essential oil

from Piper nigrum L. and Piper retrofractum Vahl.

against Callosobruchus maculatus (F.)

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Asst. Prof. Dr. Ruchuon Wanna

PT-P-007 Effect of 1-methycyclopropene (1-MCP) on

physiology and quality of fresh roselle (Hibiscus

sabdariffa Linn.) calyxs stored at low temperature

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Assoc. Prof. Dr. Maratree Plainsirichai

FS-P-001 Some factors affected on betta fish (Betta splendens)

breeding in winter season

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Asst. Prof. Dr. Panarat Phadee

BT-P-001 Antioxidant potential of rice grain processed by solid

state cultivation with Cordycep militaris

Sudarat Tasoon

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Contents

Abstracts: Page

Poster presentations:

BT-P-002 Optimization of microwave-assisted extraction of

mulberry twigs (Morus alba Linn.) on antityrosinase

and antioxidant potential using response surface

methodology

53

Waranya Kanyaprasit

BT-P-003 Cold plasma effect on mustard green microgreens in

different growth periods

54

Asst. Prof. Dr. Vijitra Luang-In


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Keynote talks


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Keynote talk 1

Thai curcuma species as functional foods

Jaehong Han *, Mi Huynh Thi Ngoc and Chaiyasarn Santipap

Metalloenzyme Research Group and Department of Plant Science and

Technology, Chung-Ang University, Anseong 17546, Korea

*Corresponding author’s email: [email protected]

Abstract: Thailand has rich plant resources applicable for the functional foods

and herbal drugs. In this talk, antioxidant and antibacterial properties, as well as

the distribution of bioactive compounds, of the 23 Curcuma species cultivated in

Thailand were presented. In details, total phenolic content and DPPH (2,2-

diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity were

determined, along with eight bioactive compounds, including curcumin (1),

demethoxycurcumin (2), bisdemethoxycurcumin (3), 1,7-diphenyl-(4E,6E)-4,6-

heptadien-3-ol (4), germacrone (5), furanodienone (6), zederone (7), and ar-

turmerone (8). While the total phenolic content of C. longa was highest (22.3 ±

2.4 mg GAE/g, mg of gallic acid equivalents), C. Wan Na-Natong exhibited the

highest DPPH radical scavenging activity. Characteristic distributions of the

bioactive compounds also guaranteed the identification and authentication of the

23 Curcuma species. C. longa contained the highest content of curcumin (1)

(304.9 ± 0.1 mg/g) and C. angustifolia contained the highest content of

germacrone (5) (373.9 ± 1.1 mg/g). It was noteworthy that 1,7-diphenyl-(4E,6E)-

4,6-heptadien-3-ol (4) was found only from C. comosa at a very high

concentration (300.7 ± 1.4 mg/g). In conclusion, it was evident that Thai

Curcuma species have a great potential as natural products.

Keywords: analysis, bioactive, Curcuma, curcuminoids, natural products


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Keynote talk 2

Migration phenomenon in food packaging: friend of foe?

Hayati Samsudin *

Food Technology Division, School of Industrial Technology, Universiti Sains

Malaysia, 11800 USM, Pulau Pinang, Malaysia


Abstract: Interaction between food and packaging materials is inevitable.

Migration is a type of interaction between food and packaging materials that

refers to the mass transfer of chemical substances from packaging materials into

the food. Migration is favorable when the migrating substances are intentionally

added as a packaging preservation technique for the food, known as active

packaging. On the other hand, migration is unfavorable when unwanted

substances such as monomer, degradation, by-products and/or contaminants as a

result of packaging manufacturing migrate into the contacting food; in this case,

this phenomenon becomes a food safety concern. A few insights and recent

updates on both aspects of the migration phenomenon will be addressed.

Keywords: migration, packaging, preservation, food safety


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Keynote talk 3

Design of pollutants screening methods in water using

photosynthetic microbes

Ling Shing Wong *

Faculty of Health and Life Sciences, INTI International University,

Persiaran Perdana BBN, Putra Nilai, 71800 Nilai,

Negeri Sembilan, Malaysia


Abstract: Water pollution is a global issue. While the global demand of

freshwater is increasing, the presence of pollutants, such as heavy metals and

pesticides are threatening the well-being of millions of human population on

earth. Conventional pollutants detection methods using high technology

equipment require high operation cost and technical skill. Thus, the development

rapid, low cost and effective screening method for pollutants detection is needed.

Single cell photosynthetic microbes, such as microalgae and cyanobacteria are

widely available in environment. These microbes are sensitive to the changes in

environment, which will react to the presence of pollutants. Utilizing the

reactions, such as mortality of cells and changes in photosynthetic behaviours,

the presence of pollutants in water can be detected. Establishing a screening

method for pollutants in water might include the identification of certain

photosynthetic microbes and reaction to be measured, immobilization of cells,

coupling the reaction to the electronic transducer, and analysis of the results to

optimize the screening method. In this session, a few designs of pollutants

screening method using photosynthetic microbes will be highlighted, with the

combination of different types of cells and transducers. The advantages and

disadvantages of each method will be discussed as well, including portability,

sensitivity, operation cost, and technical requirement for the operation.

Keywords: water pollutants, screening of pollutants, bioindicator,

photosynthetic microbes


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Keynote talk 4

Immune response and protective efficacy of a polymeric adjuvant

(Montanide™ Gel 02 PR), administered with a Streptococcus

agalactiae ghost vaccine in Nile tilapia (Oreochromis niloticus)

Regis Vialle 1*, Eakapol Wangkaghart 2, Sebastien Deville 1,

Bei Wang 3, Prapansak Srisapoome 4, Tiehui Wang 5 and Christopher J.

Secombes 5

1 SEPPIC, Paris La Defense, 50 Boulevard National, CS 90020, 92257, La

Garenne Colombes Cedex, France 2 Research Unit of Excellence for Tropical Fisheries and Technology, Division

of Fisheries, Department of Agricultural Technology, Faculty of Technology,

Mahasarakham University, Khamriang Sub-District, Kantarawichai,

Mahasarakham, Thailand 3 Guangdong Provincial Key Laboratory of Pathogenic Biology and

Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for

Disease of Aquatic Animals of Guangdong Higher Education Institutes,

College of Fishery, Guangdong Ocean University, Zhanjiang, PR China 4 Department of Aquaculture, Faculty of Fisheries, Kasetsart University,

Jatujak, Bangkok, 10900, Thailand 5 Scottish Fish Immunology Research Centre, School of Biological Sciences,

University of Aberdeen, Aberdeen, United Kingdom


Abstract: Streptococcus agalactiae is one of the most important pathogens

infecting tilapia worldwide and causes meningoencephalitis, septicemia and high

mortalities with considerable losses. Various types of vaccines have been

developed against S. agalactiae infection, such as inactivated vaccines, live

attenuated vaccines and subunit vaccines. Bacterial ghosts (BGs) are nonliving,

empty cell envelopes and have been reported as novel vaccine candidates.

Therefore, the main aims of this study were to develop an S. agalactiae ghost

vaccine (SAGV) and to evaluate the immune response and protective effect of

SAGV against S. agalactiae with one novel Polymeric adjuvant Montanide™ Gel

02 PR. Nile tilapia, mean weight 50 g, were divided into four groups as follows;

1) fish injected with PBS as control, 2) fish injected with the SAGV alone; 3)

fish injected with the SAGV+W/O adjuvant; and 4) fish injected with

SAGV+Montanide™ Gel 02 PR. Following vaccination, innate immunity

(bactericidal activity) was significantly enhanced. Moreover, specific serum IgM

antibodies were induced and reached their highest level 2-8 weeks post

vaccination. Importantly, the relative percent survival of tilapia vaccinated


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against the SAGV formulated with a polymer adjuvant was 88.5% at 8 weeks

post vaccination. In summary, Montanide™ Gel 02 PR can enhance

immunoprotection induced by the SAGV vaccine against streptococcosis,

demonstrating that it has value as a potential adjuvant of fish vaccines.

Keywords: Streptococcus agalactiae, immune response, new adjuvants,

Montanide™ GEL02, ghost vaccine, Nile tilapia


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Invited talks


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Invited talk 1

Parameter estimation for modeling of dynamic food systems

Kirk Dolan*

Department of Food Science & Human Nutrition, Michigan State University,

469 Wilson Road, East Lansing, MI 48824


Abstract: In most commercial food processes, the temperature of the product

changes continuously. However, research simulations typically use constant-

temperature experimental conditions because the data analysis methods are

simpler. This isothermal experimental method will not work for some important

processes: 1) Products heated rapidly above 100 oC (e.g. aseptic foods, waffles);

2) Low-moisture foods (e.g. flour, nuts, extruded snacks, spray-dried foods); 3)

Larger products where slow heating near the center increases resistance of the

pathogen (e.g oven-cooked meats). U.S. Federal Regulations require evidence

of a 4-log reduction of Salmonella in low-moisture foods. To meet this

requirement, the food industry can either do costly large-scale validation studies

that, or use less expensive modeling methods. Therefore, there is a need for a

modeling method that will allow researchers to estimate food-safety and -quality

modeling parameters for dynamic processes.

An innovative generic approach is given to estimate parameters from

realistic commercial dynamic data. Plotting of scaled sensitivity coefficients

(SSCs) and engineering diagnostic tools to determine which parameters can be

estimated. Once the SSCs have been interpreted, an inverse approach is used to

estimate the identified parameters in the model. Finally, statistics are reported

and a residual analysis is used determine how well the model fits the data.

Applications of this theory and method are illustrated using examples of 1)

Microbial inactivation; 2) Nutrient degradation; 3) Pathogen inactivation on

almonds; and 4) Aseptic processing of carrot particulates. Use of a novel

instrument, the TPCell, to rapidly determine temperature-dependent thermal

properties of foods at temperatures up to 140 oC is shown.

Advantages of this method include handling realistic, rapid industrial

processes, and requiring less laborious experimental setups. Although this

method requires more advanced mathematics and statistics, recent advances in

computer languages (e.g. MATLAB) and computational power make this

method more accessible.

Keywords: food safety, parameter estimation, modeling


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Invited talk 2

The role of active packaging in clean labels foods

Kirtiraj K. Gaikwad *

Department of Paper Technology, Indian Institute of Technology Roorkee,

Haridwar Highway, Roorkee, Uttarakhand 247667, India


Abstract: Buyers from developed nations are very much concerned in

particulars about the manufacturing techniques and ingredients of the food

products they eat. Some manufacturing techniques are apparent as less “natural,”

while some food ingredients/additives are considered as “unhealthy.” Such

terms, referred to as the “clean label” trend, have driven the food processing

industries to connect whether some ingredients/additives are not existing or if the

food has been produced using a “natural” production method. With customers

motivating the modernization, the food processing industries have moved toward

no or fewer ingredients using active packaging solutions. The food manufacturer

is responding by integrating active packaging. Here we review active packaging

techniques that support clean label trends focusing on driving the clean labels

market. We present active packaging technologies antimicrobial packaging;

antioxidant releaser; ethylene absorber, oxygen scavenger, carbon dioxide

emitters. We discuss the various challenges in implementing clean label foods

technology and its future in the modern society

Keywords: active packaging, clean Labels, food safety, quality, ingredients


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Invited talk 3

Breeding novelty Phalaenopsis orchids to deploy for future market

Fure Chyi Chen 1,2* and Jian Zhi Huang 2

1 General Research Service Center, National Pingtung University of Science

and Technology, Pingtung, Taiwan 2 Department of Plant Industry, National Pingtung University of Science and

Technology, Pingtung, Taiwan


Abstract: Phalaenopsis orchids are widely produced year round in a large

quantity due to their easy spiking response under proper cooling temperature.

Since diverse flower colors and pot size of desirable horticultural traits are

already available in the market, it is an urgent need to breed novel hybrids to

meet the demands of diverse markets. Our breeding program was aimed at goals

for double or multiple spikes with novel traits. Phalaenopsis species and

interspecific hybrids having multiple spikes and/or compact leaf type have been

adopted to create intermediate interspecific hybrids, including P. micholitzii and

P. tetraspis, and P. venosa, which are then used to cross hybridize with

commercial varieties to breed for novel cultivars. Other species could also be

added in the breeding program to create multiple miniature flowers, fragrance,

in addition to the indigo colored hybrids. The species such as P. equestris, P.

lindenii and P. violacea of different varieties, will contribute the fragrance and

indigo colors. Many other hobby hybrids with novelty traits are being actively

hybridized so they can be used to widen the spectrum of traits not exist in

commercial cultivars. To promote high efficiency breeding genome and

transcriptome analyses were used and the cause of the big lip was found due in

part to the alternative splicing in the MADS box gene PhAGL6b. The expression

and regulation mode of the gene may contribute clues to elucidate the lip

formation and lead to develop suitable molecular markers for increasing breeding

efficiency of the big lip hybrids in the future.

Keywords: interspecific hybrids, spike number, miniature, genome and

transcriptome, big lip


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Invited talk 4

Smart rice duck pig farming system: key to precision agriculture

Andrea N. Adriano 1, Princes Nozil T. Fabro 1, Lizel Jane M. Godoy 1

Yazmin Roey M. Nicolas 1, Antonio J. Barroga 2*, Roger J. Barroga 3

Nehemiah Caballong 3, Pamela V. Carbungco 3, Edwin M. Dicen III 3

and Austian A. Alday 3

1 College of Agriculture, Central Luzon State University

2 College of Art and Science, Central Luzon State University 3 Future Rice Farm, PHILRICE, Maligaya, Science City of Munoz, Nueva Ecija


Abstract: SMART and Precision Agriculture was demonstrated in a rice- duck

farming system raised adjacent to a 30 head capacity pig house made of rice hull

– rice straw litter bed flooring. The slurry from the wallowing pool inside the 30

m2 pig pen was continuously drained to a septic tank with 6 plastic drum biogas

digesters. A periodic application of the sludge, which is combination of rice hull,

feeds and hog manure was done in a 453 m2 paddy field herded with 54 ducks

and enclosed with meshed net fence. Moreover, the sludge and slurry from the

septic with 6 plastic drum biogas digester also served as the main nutrient source

for robust growing cash crop vegetables namely: eggplant, tomatoes, chili,

squash and pole sitao. The ducklings were 14 days old when they were

transferred to the paddy field with the rice plants already 14 days after

transplanting. An air quality sensor using AMOY software was also installed in

the pig pen to measure real time ammonia and temperature, which are vital air

quality parameters to ensure pig health, particularly in the prevention of life

threatening respiratory diseases. The pig pen was also monitored real time via

CCTV installed in android compatible phone together with the air quality sensor.

Consequently, the ducks remained in the paddy field for 56 days only or prior to

panicle initiation of the rice plant. From the ecological integration, palay yield

was demonstrated as a result of the combined and sole nutrient supplying effect

from pig based slurry and sludge and duck manure. The effective feasting nature

of ducks for insects, weeds and the golden apple snail was documented, and a

healthier environment for pigs reared from a 90 cm deep litterbed made of rice

hull and fly ash that trapped ammonia and maintained an ideal ambient

temperature for pigs in the barn. Therefore, this can be adopted as a future trend

for livestock production system with emphasis to environmental protection and

its wide scale application is highly recommended.

Keywords: ecological livestock based farming system, internet of things,

precision agriculture, rice duck farming system

mailto:[email protected]


26

Oral presentations


27

1_FN-O-001

Pharmacognostic specification and quality control of

Cannabis sativa L. subsp. sativa

Lukman Sueree 1,4,*, Fameera Madaka 1, Suchada Jongrungruangchok 2,

Niran Vipunngeun 3 and Thanapat Songsak 3

1 Drug and Herbal Product Research and Development Center, College of

Pharmacy, Rangsit University, Pathum thani 12000, Thailand 2 Department of Pharmaceutical Chemistry, College of Pharmacy, Rangsit

University, Pathum thani 12000, Thailand 3 Department of Pharmacognosy, College of Pharmacy, Rangsit University,

Pathum thani 12000, Thailand 4 Sun Herb Thai-Chinese Manufacturing, College of Pharmacy, Rangsit

University, Pathum thani 12000, Thailand


Abstract: This study provided pharmacognostic specification and quality

control of Cannabis sativa L. subsp. sativa. Macroscopic and microscopic

characteristics of crude drug were demonstrated. The loss on drying, total ash,

acid-insoluble ash, ethanol soluble extractives and water soluble extractives from

leaf which were found to be 5.12±0.13, 14.41±0.43, 1.21±0.08, 3.24±0.06 and

21.75±0.38 % by weight, respectively and from flower as 5.25±0.01, 12.61±0.14,

1.08±0.08, 4.06±0.02 and 19.51±0.27 % by weight, respectively. The ethanol

extract from leaf and flower was analysed by thin layer chromatography (TLC)

using silica gel 60 GF254 as stationary phase. In addition, hexane and ethyl acetate

were used as mobile phase. Preliminary phytochemical screening of both the leaf

and flower extract revealed the presence of various classes of compounds such

as anthraquinone, saponins, cardiac glycoside, tannin, flavonoids with major

presence of alkaloid. The crude drug showed contamination by total bacteria and

yeast/mold, whereas no presence of Escherichia coli, Clostridium spp.,

Salmonella spp. Staphylococcus aureus and Pseudomonas aeruginosa. From

analysis of heavy contamination, was found that crude drug contained heavy

metal at safe levels.

Keywords: Cannabis sativa L. subsp. sativa, TLC, pharmacognostic

specification, quality control, phytochemical screening


28

2_FN-O-002

The study of LPG drying process for sliced pork

using mathematical modeling

Penpark Sirimark 1, Sanchai Yotmanee 2

and Praphanpong Somsila 3*

1Department of Science and Mathematics, Rajamangala University of

Technology Isan Surin Campus, Surin, Thailand 2Faculty of Science and Technology, Phuket Rajabhat University, Phuket,

Thailand 3Department of Mechanical Engineering, Rajamangala University of

Technology Isan Surin Campus, Surin, Thailand


Abstract: The sliced pork drying process using LPG hot air dryer was

investigated with 3 different drying temperatures (50, 55, and 60°C) and 2

different air velocities (1 and 2 m/s). The data was fitted to determine the best

mathematical model. Drying models including the Lewis model, Page model,

Henderson and Pabis model, and Wong and Singh model were evaluated to

describe the drying kinetics under the different conditions. The results showed

that the moisture of sliced pork decreased with an increase in drying temperature.

The drying characteristics were best described by Henderson and Pabis model

due to a high coefficient of determination (R2=0.9995), less sum of squares due

to error (SSE=0.0007), and root mean square error (RMSE=0.0063). The drying

rate curve showed that sliced pork drying occurred during a period which

significantly influenced moisture diffusion during the drying. Effective moisture

diffusivity (Deff) values were in the range of 6.48 × 10-9 - 1.91×10-8 m2/s. The

minimum activation energy for LPG hot air dryer was 84.89 kJ/mol.

Keywords: mathematical modelling, LPG hot air dryer, drying process


29

3_FN-O-003

Development of novel pH sensitive oxygen indicator based on Gallic

acid for food safety and security

Akhila Konala 1*, Dakuri Ramakanth 2, Vidhi Gupta 1 and

Kirtiraj K. Gaikwad 1

1 Department of Paper Technology, Indian Institute of Technology Roorkee,

India 2 Department of Polymer and Process Engineering, Indian Institute of

Technology Roorkee, India


Abstract: The increased consumer demand on food quality assurance has

evoked a great interest to develop techniques that aid in visualizing the quality

changes in food with time when exposed to gases, moisture and light. Intelligent

packaging can play a vital role in communicating this information to the ultimate

consumer. Oxidative reactions such as lipid oxidation, protein degradation is

responsible for the deterioration of food. Hence to address these issues, this work

proposes the development of gallic acid (GA) based pH sensitive oxygen

indicators. The coating solution was formulated using cellulose acetate (binder),

ethyl acetate/ethanol (solvent) and gallic acid (active agent) as 5, 10, 20 and 30

wt%. The solution was bar coated onto paper substrate (110 g/m2), dried at

ambient conditions and characterized using FT-IR, UV-Visible, FE-SEM, AFM

and L*, a*, b*. Upon exposure to varying pH at atmospheric oxygen, the coated

paper exhibited a color change from green to yellow for a pH range 8-12 as

evident from UV-Visible spectra at 400-800 nm. AFM was performed for surface

roughness of the coating. Rheological studies showed GA20% has better

viscosity for coating application, FE-SEM images confirmed GA 20% has

smoother surface. ΔE values has confirmed that GA30% has highest color

difference. Therefore, gallic acid has a potential to be used as a pH sensitive

oxygen indicator in food packaging applications.

Keywords: oxygen indicators, gallic acid, intelligent packaging, pH sensitive,

coated paper, food packaging


30

4_FN-O-004

Development of bioplastic films into functional packaging for food

products

Nathdanai Harnkarnsujarit *, Pattarin Leelaphiwat and

Nattinee Bumbudsanpharoke

Advanced Functional Packaging for Agriculture and Foods (AFPA),

Department of Packaging and Materials Technology, Faculty of Agro-Industry,

Kasetsart University, Bangkok, Thailand


Abstract: Global policy on reduction of food loss and packaging waste are

primary aims to achieve sustainability in food and packaging industry. Bioplastic

materials including poly(lactic acid) (PLA), thermoplastic starch (TPS) and

poly(butylene adipate terephthalate) (PBAT) have high potential to produce food

packaging which reduce the environmental impact of packaging waste. However,

poor physical and barrier properties of these materials are major drawbacks

which limit utilization as food packaging. Therefore, incorporation of

antimicrobial and antioxidant function into bioplastic possibly compensate for

poor barrier and create high value to bioplastic polymers. The present study

developed the bioplastic incorporating functional ingredients from TPS and

PBAT based materials. The films were produced by conventional extrusion

process and determined for the effects of incorporated functional ingredients on

packaging properties including tensile properties, water vapor and oxygen

barrier. Films were also used as packaging for food products and several product

qualities were determined during storage. Findings indicated that incorporation

of functional ingredients modified morphology of the film matrices which

subsequently influenced properties of bioplastic film materials. Moreover,

incorporation of functional ingredients through polymer compounding via

extrusion produced functional active packaging which also limited microbial

growth which prolonged shelf-life of packaged foods.

Keywords: food packaging, active packaging, bioplastic, film, shelf-life


31

5_FN-O-005

Functional packaging of meat products from nisin and EDTA

incorporated films

Nathdanai Harnkarnsujarit *, Pattarin Leelaphiwat and

Nattinee Bumbudsanpharoke

Advanced Functional Packaging for Agriculture and Foods (AFPA),

Department of Packaging and Materials Technology, Faculty of Agro-

Industry, Kasetsart University, Bangkok, Thailand


Abstract: Functional packaging for food products gave extra benefits beyond

conventional packaging. Incorporated antimicrobial agents into polymers

possibly enhances antimicrobial capacity and produces functional packaging.

This research produced films by compounding nisin and nisin-

ethylenediaminetetraacetic acid (EDTA) mixtures using extrusion. Nisin and

EDTA plasticized the films and modified morphology, while improved barrier

properties due to alteration of hydrophilic-hydrophobic properties and

morphology. The functional films also restricted growth of microorganisms,

particularly in the presence of EDTA, giving stabilized redness and delayed meat

discoloration. Qualities of pork was better preserved. Accordingly, interaction

between nisin, EDTA and polymers altered the morphology and subsequent

properties, producing functionalized biodegradable food packaging.

Keywords: food packaging, active packaging, meat, nisin, EDTA


32

6_BT-O-001

Riceberry broken rice and soybean meal as substrates for

exopolysaccharide production by Bacillus tequilensis PS21

Thipphiya Karirat, Worachot Saengha, Sirirat Deeseenthum and

Vijitra Luang-In *

Natural Antioxidant Innovation Research Unit, Department of Biotechnology,

Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai,

Maha Sarakham 44150, Thailand.


Abstract: This work aimed to study the effect of riceberry broken rice and

soybean meal on exopolysaccharide (EPS) production by Bacillus tequilensis

PS21. Various riceberry broken rice (RBR) as carbon source at 4, 5, and 6%

soybean meal (SBM) as nitrogen source at 1, 2, and 3% were used whilst the

fermentation conditions at 37 °C and media pH 7 were constant. The results

showed that as fermentation time increased from 24, 48 to 72 h, the EPS content

increased significantly along with bacteria growth. RBR (4, 5, and 6%) at 72 h

resulted in no significant differences in EPS production. However, SBM at 1, 2,

and 3% resulted in a statistically significant difference in EPS content at 72 h.

Therefore, the optimized conditions for EPS production were RBR at 5% and

SBM at 3% for an EPS content of 28.47 g/L. This work demonstrated a new way

to add value to RBR and SBM to produce an EPS bioproduct for future

applications.

Keywords: Bacillus tequilensis, exopolysaccharide, riceberry broken rice,

soybean meal


33

7_BT-O-002

Optimization of Arthrospira platensis growth using organic culture

medium

Aporn Bualuang 1, Bongkoj Boonburapong 2 and Surasak Laloknam 2*

1 Chulabhorn International College of Medicine, Thammasat University,

Pathumthani 12120, Thailand 2 Department of General Science, Faculty of Sciences, Srinakharinwirot

University, Wattana District, Bangkok 10110, Thailand


Abstract: In this study, the organic cultivation medium for Arthrospira platensis

(Spirulina) was produced using sugarcane molasses (SGCM), soybean meal

(SBM), and small fish bone meal (SFBM) as substitution of macronutrients and

micronutrients in Zarrouk’s medium. Total reducing sugar and total protein

content were determined in SGCM, SBM, and SFBM. SGCM showed the

highest total reducing sugar, followed by SBM and SFBM, respectively. SBM

showed the highest total protein, followed by SFBM, while protein was not

present in SGCM. The single material was designed to substitute macronutrients

and micronutrients in Zarrouk’s medium. In the SGCM medium, the total

reducing sugar was varied concentration range 0 to 3.0% (w/v), which was

similar with the total protein in the SBM medium. The percentages of dried

SFBM in the SFBM medium were also concentration range 0 to 1.0 % (w/v).

Cell growth of A. platensis was monitored every three days for 30 days by using

spectrophotometer at absorbance 750 nm. The results showed that the optimum

concentrations of the SGCM, SBM and SFBM media were 0.2% (w/v), 0.5%

(w/v) and 0.1% (w/v), respectively. The combination medium was designed

using the optimal concentration of SGCM, SBM, and SFBM. Cell growth of A.

platensis was investigated and compared with Zarrouk's medium for 30 days.

The yield of A. platensis in combination medium and Zarrouk's medium were

31.25 g/L and 33.46 g/L, respectively. This finding indicated that the

combination medium composed of organic substance medium could be used as

cultural media of A. platensis for the organic system.

Keywords: Arthrospira platensis, organic cultivation medium, small fish bone

meal, soybean meal, sugarcane molasses


34

8_PT-O-004

Bio-water hyacinth (Eichhornia crassipes) fermented extracts

supplemented plantation pots promote growth

of water spinach (Ipomoea aquatica)

Bongkoj Boonburapong 1, Jittiwat Kaewdee 1, Sirapatsorn Wanriko 1,

Kun Silprasit 2, and Surasak Laloknam 1*

1 Department of General Science, Faculty of Sciences, Srinakharinwirot

University, Wattana District, Bangkok 10110, Thailand 2 Faculty of Environmental Culture and Ecotourism, Srinakharinwirot

University, Ongkharak District, Nakhon Nayok 26120, Thailand


Abstract: This research aimed to apply water hyacinth (WH; Eichhornia

crassipes) as a resource in agricultural application, WH pots supplemented with

WH bio-fermented extract, and study the effect of WH pots supplemented with

WH bio-fermented extract on the growth of water spinach (Ipomoea aquatica).

WH leaves were used to prepare bio-fermented extracts and WH stems were used

to prepare cellulose fiber for cellulose pot production. Firstly, the optimization

of WH contents (0, 10, 20, 30, 40, and 50 g) for bio-fermented extracts

production was determined based on NPK contents. The result revealed that bio-

fermented extracts containing 50 g of WH leaves had the highest tendency of

NPK contents. The WH cellulose fiber was prepared using 3 conditions: 1) boiled

in tap water 2) boiled in 10%(w/v) NaOH, and 3) boiled in 10%(w/v) NaOH and

treated with 1%(v/v) NaOCl. All cellulose fibers were subjected to determine the

NPK absorption and releasing activities. The results showed the highest NPK

absorption and releasing activities is cellulose fiber prepared with boiling in tap

water which was subjected to WH pots preparations. Next, the effect of WH pots

supplemented with bio-fermented extracts on the growth of water spinach was

investigated comparing with WH pots without bio-fermented extracts and plastic

pots. The results showed that the WH pots supplemented with bio-fermented

extracts promoting the growth of water spinach better than control. This finding

showed that water hyacinth biomass could be used as a source of plant nutrients

and eco-friendly planting pots.

Keywords: bio-fermented extract, cellulose fiber, water hyacinth


35

9_PT-O-005

Effects of bio-extracts from cyanobacteria Nostoc sp. on growth and

quality of Brahmi (Bacopa monniera)

Surasak Laloknam 1*, Salinee Tengnoy 1, Waranya Kaewkham 1,

Bongkoj Boonburapong 1 and Aporn Bualuang 2

1 Department of General Science, Faculty of Sciences, Srinakharinwirot

University, Wattana District, Bangkok 10110, Thailand 2 Chulabhorn International College of Medicine, Thammasat University,

Pathumthani 12120, Thailand


Abstract: This research aimed to investigate the effects of bio-extracts on the

growth and quality of Brahmi (Bacopa monniera) growing in a hydroponic

system. The experimental design was RCBD (Randomized Complete Block

Design) with 5 treatments and 3 replications including control solution (Bristal

medium), bio-extract of 0.001%(w/v) Nostoc sp. (NT), bio-extract of

0.01%(w/v) NT, bio-extract of 0.1%(w/v) NT, and bio-extract of 1.0%(w/v) NT,

preparing the EC of 1.4 mS/cm and pH between 5.8 to 6.0. This experiment was

conducted at the Department of General Science, Faculty of Science,

Srinakharinwirot University. The growth survival rate, shoot height, root length,

bud numbers, and chlorophyll contents were investigated. The cyanobacteria

Indole acetic acid (IAA) contents were determined. The result showed that the

vegetative yield of Brahmi treated with bio-extract of 0.01% (w/v) NT was the

highest survival rate, shoot height, root length, bud numbers, and chlorophyll

content. We found that IAA concentration in bio-extract of 0.01% (w/v) Nostoc

sp. is 4.15 µg/ml extract. This finding suggests that cyanobacteria extract could

be used as a source of plant growth regulators that promote the Brahmi growth

in the hydroponic system.

Keywords: bio-extract, Brahmi, growth, IAA, Nostoc


36

10_PT-O-001

Effects of probiotic on corn silage quality and rumen digestibility

Chanya Plumejai, K.Teepalak Rangubhet * and Phongthorn Kongmun

Department of Animal Science, Faculty of Agriculture, Kasetsart University,

Bangkok, 10900


Abstract: The current study aims to evaluate the silage quality by somatic

senses, nutrient composition by proximate analysis, and digestibility by in vitro

true digestibility of Hybrid-Suwan 4452, 5731 and 5819 corns ensiled with

Bacillus subtilis (BS) 1 x 105 cfu/g for 0, 21, 30, 45, and 60 days. The experiment

data were designed in to 2 x 2 factorials in RCBD consisting of two factors which

were inoculation (untreated and B. subtilis) and ensiling periods (0, 21, 30, 45,

and 60 days). The result showed that Hybrid-Suwan 4452, 5731 and 5819 corns

ensiled with Bacillus subtilis 1 x 105 cfu/g could maintain silage quality up to

day 60th which could be the alternative method for farmers in the insufficient of

roughage condition. Meanwhile, the nutrient compositions were appropriate for

ruminant utilization as dry matter contents were significantly increased in

Hybrid-Suwan 4452 corn that ensiled with BS for 30 days (P < 0.05). Cellulose

contents were significantly increased by time of fermentation in Hybrid-Suwan

5819 corn ensiled with BS. Lactic acid contents were significantly increased in

Hybrid-Suwan 5819 corn ensiled with BS at day 0, 21st, and 30th. The

digestibility of nutrient in terms of dry matter and organic matter were increased

in Hybrid-Suwan 4452 and 5731 corns ensiled with BS at day 21st and 30th of

ensiling (P < 0.05). In conclusion, ensiling corn silage with BS was valuable to

maintain the silage quality for enlargement storage time and effective to enhance

the nutrient compositions and nutrients digestibility in rumen.

Keywords: Bacillus subtilis, corn (Zea mays), hybrid-Suwan 4452 corn, Hybrid-

Suwan 5731 corn, hybrid-Suwan 5819 corn, in vitro true digestibility, silage


37

11_PT-O-002

Identification and transcriptional analysis of the metal tolerance

protein (MTP) gene family in cassava under zinc deficiency

Natlita Payap, Triwarat Rujikiadtichok and

Nimnara Yookongkaew *

Department of Biology, Faculty of Science, Silpakorn University, Nakhon

Pathom, Thailand


Abstract: Available of zinc in cassava plants is essential for plant developmental

stages as well as crop production. Here, the molecular mechanism of zinc

transport in cassava plants was explored. We found zinc deficient symptom in

upper cassava leaves under zinc deficiency and identified the metal tolerance

proteins (MTPs) family in cassava genome. MTPs are a metal cation efflux

transporter that participated in zinc homeostasis. Computational analysis showed

12 MeMTP members in cassava genome with exons ranging from 1-12. Most of

the MTP proteins were predicted to localize in plasma membrane and tonoplast

except MeMTP4 and MeMTP8, which localized in ER membrane. Functional

annotation verified that MeMTPs were cation efflux proteins/zinc transporters

belonging to cation diffusion facilitator superfamily (CDF). Most of the cis-

acting elements in MeMTP promoters were phytohormone responsive. We

identified TGA-element in MeMTP2, MeMTP5-7 and MeMTP10 promoters,

indicating its role in auxin regulation. MeMTP proteins were divided into three

groups according to phylogenetic relationship. Moreover, RNA expression of 6

candidate MeMTP genes including MeMTP1-5 and MeMTP12 was evaluated

under zinc deficiency. MeMTP1 was up-regulated in roots and leaves under zinc

deficiency whereas the expression of MeMTP2, MeMTP4 and MeMTP12 were

tissue-specific. These findings will provide an important foundation of the MTPs

in zinc homeostasis mechanism of cassava plants.

Keywords: zinc, zinc deficiency, cassava, metal tolerance proteins, gene

expression


38

12_PT-O-003

Effect of electromagnetic field on root cassava cv. Rayong 72

Taweesab Chaiyarak 1*, Naris Sinsiri 1, Teerawong Laosuwan 2 and

Phirayot Khaengkhan 1

1Department of Agricultural Technology, Faculty of Technology,

Mahasarakham University,Maha Sarakham, 44150, Thailand 2Department of Physics, Faculty of Science, Mahasarakham University,

Maha Sarakham,44150, Thailand


Abstract: The Electromagnetic field is electrical energy that occurs in nature.

There are always free charges that influence the evolutionary process of living.

It is a safe and new alternative way to develop crop production. This investigation

aimed to study optimized conditions using time and electromagnetic field for

root development of cassava cv. Rayong 72. The 4x3 factorial arrangement in

the completely randomized design with 4 replications. There were 2 factors

consisting of 1) 4 times of stimulations: 0, 15, 30, and 45 minutes, and 2) 3 levels

of the electromagnetic field: 30, 40, and 50 mT. The root physiology (total root

length, total root surface area, root length/volume, root volume, root tips, root

average diameter, root width, and root surface area) were measured to test the

effect of the electromagnetic field at 30 days after plant under greenhouse

condition. The results showed that the interaction between using time and

electromagnetic field intensity at 45 min with 30, 40 and 50 mT of

electromagnetic intensity resulted in the highest of total root length, total root

surface area, root length/volume, root volume, number of root tips, root diameter,

root width and surface area of cassava. In addition, changes in total root length

were correlated with total root surface area (r = 0.7295 **), root length per

volume (r = 0.6509 **), root volume (r = 0.6539 **), root tip number. (r = 0.6919

**), root diameter (0.3149 *), root width (r = 0.1197), and root surface area (r =

0.7130 **), while there was negative correlation between root length and the

increased volume. This resulted in a decrease in root width (r = -0.0360) and an

increase in diameter that had an effect on root width (r = -0.1000).

Keywords: electromagnetic field, cassava, growth, root physiology


39

Poster presentations


40

FN-P-001

Physical alteration of the peeled durian stored in “OZONE BOX”

odor lock packaging

Siriwan Tungsangprateep 1*, Waree Jaruwattanayon 1, Kasinee

Saowakon 2, Papitchaya Kongchinda 2 and Rujira Deewatthanawong 2

1 Thailand Institute of Scientific and Technological Research 196 Phahonyothin

Rd., Ladyao, Chatuchak, Bangkok 10900 2 Thailand Institute of Scientific and Technological Research 35 Mu 3, Khlong

Luang, Pathum Thani 12120, Thailand


Abstract: This study investigated the physical changes including oxygen (O2)

and carbon dioxide (CO2) gas content, weight loss, color change and firmness of

peeled durian stored at 4 °C for 15 days in “Ozone Box” odor locking package

compared to commercial package. Testing plan was a Complete Randomized

Design (CRD) experiment. Statistical analysis was conducted by comparison of

the mean t-test at 95% confidence level. It showed that the oxygen gas in Ozone

Box decreased significantly when compared to commercial package. On the

other hand, carbon dioxide increased dramatically in the first 5 days of storage.

It has been indicated that Ozone Box has complete barrier properties against

ingress of oxygen and carbon dioxide through the development of the air tight

seal structure. The durian in Ozone Box has less weight loss than commercial

package in 5-7 days of storage. This was because Ozone Box absolutely retained

moisture inside the air tight seal structure, while the commercial package had a

seal opening, so moisture could not be contained. For color change and firmness,

there was no significant difference in the durian in both packages throughout the

storage period.

Keywords: odor lock packaging, durian, OZONE BOX


41

FN-P-002

Phytochemical, antioxidant activity and color parameter from seed

extract of Thai black rice in different seasons

Muntana Nakornriab 1*, Phanchaloen Nuengruethi 1, Suwanan

Yodphikul 1, Chalermchai Trakhamhan 1 and Jeraporn Krasaetep 2

1Department of Chemistry, Faculty of Science, Mahasarakham University,

Mahasarakham, 44150, Thailand 2Department of Agricultural Technology, Faculty of Technology,

Mahasarakham University, Mahasarakham, 44150, Thailand


Abstract: The objectives of this work were to study phytochemical compounds

(total phenolic, flavonoid, anthocyanin contents), antioxidant activities and

parameters affecting on Thai black rice seed colours cultivars in the different

seasons. Total phenolic and anthocyanin contents were measured by using Folin-

Ciocalteu and pH differential methods respectively. The antioxidant activities

were measured by using DPPH and FRAP assays. The colours of rice cultivars

were classified using color parameters; lightness (L*), redness (a*), yellowness

(b*), chroma (C), and hue angle (Ho). The results showed that the rice-berry

extract from wet season 2019 had the highest total phenolic and anthocyanin

contents. The rice-berry extract in wet season 2018 showed the highest activity

by DPPH (IC50 = 10.17 ± 0.32 mg/mL), while the Nong Khai black jasmine rice

in dry season 2019 and rice-berry extract in dry season 2019 had the highest

FRAP values. All black rice samples decreased in lightness (L*), while the

positive a* values or redness were found in all rice samples. The negative b*

values or blue were found in rice-berry, while positive b* values were found in

Nong Khai black jasmine rice. In conclusion, the Thai black rice should be

proposed as potential sources of safe natural antioxidants and preservatives for

rice industry.

Keywords: Thai black rice, phytochemical, antioxidant, colour parameter,

seasons



42

FN-P-003

Development of active film using agricultural by-products: pomelo

peel and karanda pomace

Sunisa Roidoung *, Phawadee Phannarungsee and Sarocha Sanudchai

Department of Food Technology & Nutrition, Faculty of Technology,

Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham,

Thailand 44150


Abstract: Application of agricultural by-products in active packaging is

currently being of interest among researchers. Utilizing by-products is not only

reducing waste, but polyphenols in agricultural by-products also enhance

properties of biopolymer, and hence benefits in shelf-life extension of packaged

products. The purpose of this study was to incorporate pomelo peel (mesocarp,

the white layer) and karanda pomace for active film development. The results

presented that film thickness of all samples was not different (p > 0.05). Adding

karanda pomace rendered darker film with high opacity, increased water vapor

permeability, and decreased water solubility (p < 0.05). Meanwhile, film with

pomelo peel showed the lowest in water vapor permeability (0.0022

g·mm/m2·h·Pa). The solubility, color and opacity were similar to control films.

Antioxidant activities of the film significantly increased (57.89-83.65%) with

addition of pomelo peel and karanda pomace. Active film with pomelo peel

provided similar physical characteristics to control film, whereas dark active film

with karanda pomace would benefit in light transmission. According to

biodegradability observation, the active films with pomelo peel and karanda

pomace showed no significant different from control films.

Keywords: active film, agricultural by-products, antioxidant, biodegradability,

karanda pomace, pomelo peel


43

PT-P-001

The pretreatment condition for chromosome count and karyotype

analysis of Dimocarpus longan from Thailand

Panurat Pipatchananan 1,2, Pathrapol Lithanatudom 2,3, Isara Patawang 2

and Suparat Kunkeaw Lithanatudom 4*

1Graduate Master’s Degree Program in Biology, Department of Biology,

Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand 2Department of Biology, Faculty of Science, Chiang Mai University,

Chiang Mai, 50200, Thailand 3Research Centre in Bioresources for Agriculture, Industry and Medicine,

Chiang Mai University, Chiang Mai, 50200, Thailand. 4Program in Genetics, Faculty of Science, Maejo University, Chiang Mai,

50290, Thailand


Abstract: Chromosome number and karyotype analysis reveal an important

information for plant evolutionary study. However, very few studies conducted

on cytogenetics of Dimocarpus longan (Longan), an economic important

subtropical fruit in Thailand. Therefore, this research aimed to develop the

practical procedure for longan chromosome preparation to investigate the

chromosome number and karyotype analysis in 8 longan cultivars from Thailand.

The chromosome preparation was optimized using longan shoot tip and root tip

cells. Two pre-treatment chemicals which are p-dichlorobenzene and 8-

hydroxyquinoline were selected and treated at 4°C in different time points

consisted of 1, 3, 6 and 24 hours. All treated samples were then hydrolysed in

1N HCl (7 minutes at 60°C) and stained with carbol fuchsin (15 minutes at room

temperature). The result showed that root sample pre-treated with p-

dichlorobenzene at 4°C for 1 hour was the best condition for longan chromosome

analysis. This condition was further used for chromosome investigation in 8

Thai’s longan cultivars which are Baiyoke, Plueakkhao, Phetsakorn, Krob-Ka-

Ti, Haewkrae, E-daw, BiewKhiew Chiangmai and Pingpong. The chromosome

number of all studied cultivars was determined as 2n=30. The variation of the

total chromosome length (varied from 0.499 to 1.293 µm) while the varied

relative length (RL) and size of chromosomes (group L, M and S) were observed

among 8 longan cultivars. According to this study, the pre-treatment method can

be used to investigate chromosomes of all longan cultivars in this research.

However, further chromosome investigation using approaches such as FISH

would be more informative for our future studies.


44

Keywords: chromosome preparation, chromosome number, karyotype,

Dimocarpus longan


45

PT-P-002

Insecticidal fumigant and repellent activities of seeds essential oils

from carrot (Daucus carota L.) against maize weevil (Sitophilus

zeamais Motschulsky)

Ruchuon Wanna *, Mongkol Wongsawas and Darika Bunphan

Department of Agricultural Technology, Faculty of Technology,

Mahasarakham University, Maha Sarakham 44150, Thailand


Abstract: The maize weevil, Sitophilus zeamais Motschulsky, is the most

destructive insect pest of whole grains, both for breeder seed and preserved

consumption. Fumigation by essential oil from carrot Daucus carota L seeds can

protect grains and products from insect pests. Insecticidal fumigation toxicity

and repellent effect of essential oil from D. carota seeds against adult S. zeamais

were evaluated. All bioassays were vapor phase tested. Results showed that

essential oil from D. carota seeds had LC50 of fumigation toxicity to adult S.

zeamais at 24, 48 and 72 hours with 26.27, 19.60 and 13.27 µL/L air,

respectively. Efficiency of 100% mortality of adult S. zeamais with 64 µL/L air

after treatment at 72 hours was significantly different (p < 0.01). Essential oil of

D. carota seeds had no repellent effect on adult S. zeamais, with an attractive

effect of less than 50% repellent efficiency. Essential oil from D. carota seeds

was highly toxic to adult S. zeamais by fumigation and showed possible use as

an alternative treatment for stored grain protection against insect pests.

Keywords: insecticides, toxicity, Daucus carota, Sitophilus zeamais, essential oil


46

PT-P-003

The effect of nutrients deficiencies on Cannabis sativa L. leaves

Natphatsorn Phiwatkunwarut, Tarit Chewsuwan and Pranot Maniin *

Department of Agricultural Innovation and Food, Faculty of Agricultural

Innovation University of Rangsit, Pathumthani, 12000, Thailand

*Corresponding author’s email: [email protected],

Abstract: A plant does not acquire enough nutrients, its must become nutrients

deficiency state, signs of deficiency are variants depending on the nutrients.

Common sign would be stunted growth, yellowish leaves (chlorosis), living

organism's cells or tissues death or degenerate (necrosis). Understanding nutrient

deficiencies and how to correct them is a skill that allows growers to improve

plant health. This research studied the effect of nutrients deficiency in cannabis

sativa L. which was grown in an indoor greenhouse with hydroponics. The

studies were in the same environment with Completely Randomized Design

(CRD) experiments with 8 treatments, including non-N, non-P, non-K, non-Ca,

non-Fe, non-Mg, non-S and complete, 2 replications in total of 16 experimental

units. The result of nutrients deficiency state differed amonst the nutrients.

Chlorosis occurred in the nitrogen, iron, potassium, calcium, magnesium, and

sulphur deficiencies. Necrosis manifested in phosphorus deficiency. It is also

divided into groups that have symptoms that occur in young leaves first,

including deficient in calcium, iron, and sulphur, and the last group with

symptoms of older leaf are plants lacking nitrogen, potassium, phosphorus,

magnesium.

Keywords: nutrient deficiency, cannabis sativa L., Hydroponics


47

PT-P-004

Effect of rates and application methods of zinc on growth, yield and

economic return of cassava variety Kasetsart50 cultivated in a

loamy sand soil

Benjapon Kunlanit 1,2*

1 Department of Agricultural Technology, Faculty of Technology,

Mahasarakham University, Maha Sarakham 44150, Thailand 2 Soil Organic Matter Management Research Group, Khon Kaen University,

Khon Kaen 40002, Thailand


Abstract: The objective of this study was to investigate effect of management

and rates of zinc (Zn) on growth, yield, quality and economic return of cassava

variety Kasetsart50. An experimental design was a randomized complete block.

There were 3 replications with 8 treatments, including 1) no fertilizer (T1), 2)

chemical fertilizer formula 15-15-15 at the rate of 313 kg ha-1 (CF) (T2), 3)

soaking stake in 2% ZnSO4.7H2O solution + CF (T3), 4) soaking stake in 4%

ZnSO4.7H2O solution + CF (T4), 5) soaking stake in 6% ZnSO4.7H2O solution

+ CF (T5), 6) incorporating ZnSO4.7H2O rate of 6.25 kg ha-1 + CF (T6), 7)

incorporating ZnSO4.7H2O rate of 12.5 kg ha-1 + CF (T7), and 8) incorporating

ZnSO4.7H2O rate of 18.75 kg ha-1 + CF (T8). The cassava was grown in a loamy

sand soil of a farmer field at Phochai district, Roi-Et province in Northeast

Thailand. The results showed that the T8 had higher plant height (196.7 cm),

tuber fresh yield (45.8 t ha-1), starch content (28.6%), and economic return over

fertilizer cost (57,707 THB ha-1) than the other treatments. It indicated that Zn

application at the rate of 18.75 kg ZnSO4.7H2O ha-1 together with chemical

fertilizer can be an alternative way to enhance yield, quality, and economic return

of cassava variety Kasetsart50 production in a loamy sand soil.

Keywords: cassava, zinc, sandy soil, chemical fertilizer, economic return


48

PT-P-005

Effects of cannabis lines on nutritional value and bioactive

ingredient compounds cannabis leaves (Cannabis sativa L.)

Yolrawee Tongsak, Kasideth Onsri and Pranot Maniin *

Faculty of Agricultural Innovation, Rangsit University, Thailand


Abstract: The objective of the study was to know the nutritional benefits and

bioactive compounds of cannabis as evidence to support the proper consumption

of cannabis. Five cannabis lines, which were RSU01, RSU08, RSU09, and

RSU12, were used in this nutrient analysis viz moisture, ash, fat, protein, fiber,

carbohydrates and energy, and bioactive compounds viz chlorophyll,

antioxidants, phenolic compounds, and anthocyanin, with the results were

analyzed by statistical methods, by giving a statistically significant difference

(P<0.05). The results showed that each strain of cannabis (100 g) with nutritional

benefits and bioactive substances as follows: moisture content 67.36-73.04 g, ash

content 5.37-9.39 g, fat content 7.30-11.40 g, protein content 9.12-11.46 g,

nitrogen content 1.46-1.83 g, fiber content 2.45-6.14 g, carbohydrate content

3.32-6.13 g, energy 122.86-163.21 kcal, chlorophyll A content 34.34-49.22

mg/cm2, chlorophyll B content 11.47-12.58 mg/cm2, total chlorophyll content

45.80-61.79 mg/cm2, antioxidant content 9.48-24.61 EC50.gL-1, phenolic

compounds content 2.38-2.57 mgGAE.100gFW-1, and anthocyanin content 7.04-

13.02 mgGAE.100gFW-1. Content of Cannabigerolic acid 0.07% /w/w), Cannabinol

0.03% (w/w), Δ9- Tetrahydrocannabinol 0.13% (w/w), and Δ9-

Tetrahydrocannabinolic acid 1.23% (w/w).

Keywords: cannabis, nutritional benefits, bioactive compounds



49

PT-P-006

Chemical composition and bioactivity of essential oil from Piper

nigrum L. and Piper retrofractum Vahl. against Callosobruchus

maculatus (F.)

Ruchuon Wanna *, Mongkol Wongsawas and Darika Bunphan




Abstract: Plants with insecticidal properties can be used as a source of these

substances for use in insect pest control. Chemical composition and bioactivity

of essential oils from dried seeds and fresh leaves of Piper nigrum L. and Piper

retrofractum Vahl. on adult of Callosobruchus maculatus (F.) were conducted.

The essential oils from dried seeds and fresh leaves of P. nigrum and P.

retrofractum showed the chemical compounds between 22-27 components

(90.34-93.27%). The major compounds in dried seeds and fresh leaves of P.

nigrum and P. retrofractum were alpha-bergamotene, caryophyllene, beta-

selinene, germacrene D, naphthalene, undecane, 5-octadecene, cyclohexene,

limonene, caryophyllene, 2-beta-pinene. Both essential oils had the same four

compositions, including limonene, linalool, caryophyllene and naphthalene.

These components had insecticide properties. In addition, the essential oils from

dried seeds of P. nigrum and P. retrofractum were more effective in the

inhibition oviposition and adult emergence of C. maculatus than the essential oils

from their fresh leaves. P. nigrum essential oil from dried seeds at concentration

of 5 µL/L air was the highest efficiency of oviposition inhibition with 55.17%

and adult emergence inhibition with 71.09% on C. maculatus. However, the

results suggested that the essential oils of P. nigrum might be used as an

alternative for grain protection against C. maculatus in the storage product.

Keywords: essential oil, Piper sp., oviposition, stored product insect


50

PT-P-007

Effect of 1-methycyclopropene (1-MCP) on physiology and quality

of fresh roselle (Hibiscus sabdariffa Linn.) calyxs stored

at low temperature

Maratree Plainsirichai *, Sonchai Homgsri, Adison Chansom


Mahasarakham University, Mahasarakham, 44150, Thailand


Abstract: Rosell (Hibiscus sabdariffa Linn.) is an annual crop providing good

natural colour and flavor mostly from anthocyanin, malic acid, and other

bioactive compounds. However, after harvesting the quality of roselle calyx

decreased rapidly, possibly from the production of ethylene. This research, was,

therefore, studied on the fumigation time of 1-MCP, ethylene inhibitor, on fresh

roselle calyx. 1-MCP at concentration of 1,500 nl/l was fumigated to roselle

calyx for 8, 12, 16, and 20 hrs before wrapping with Polypropylene (PP) in a

comparison with control (no 1-MCP and no wrapping) and they were stored in a

refrigerator at 15 °C, 80 % RH. It demonstrated that roselle calyx fumigated with

1,500 nl/l 1-MCP for 8 hrs had weight lost only 8.75 % at day 14 significantly

less than other fumigation times at 12 hrs (12.64 %), 16 hrs (14.63 %), 20 hrs

(16.45%), and without 1-MCP and without wrapping (control) (70.31 %).

Furthermore, the rosell calyxs treated with 1-MCP at every fumigation time had

rather constant of titratable acidity and total soluble solid from day 0 to day 14.

The roselle calyxs obtained 1,500 nl/l 1-MCP for 8 hrs and 12 hrs had decay

incidence not significantly different from each other but significantly lower than

other treatments at every storage time (day 4, 6, 8, 10 and 12). At day 14, the

roselle calyxs obtained 1,500 1-MCP for 8 hrs contained anthocyanin (0.49

mg/gFW) significantly higher than other treatments obtained 1,500 1-MCP at

other fumigation times but not significant difference with the roselle calyxs in

control (0.52 mg/gFW). The application of 1,500 nl/l 1-MCP at 8, 12, 16, 20 hrs

resulted in the significantly higher storage life than control (13.75, 12.50, 12.00,

10.25 days respectively) (3.75 days in control). In conclusion, the application of

1,500 nl/l 1-MCP for 8 hrs reduced weight loss and decay incidence, maintained

titratable acidity, total soluble solid, anthocyanin, and increased storage life of

fresh roselle calyxs.

Keywords: Roselle, Hibiscus sabdariffa Linn., 1-MCP, anthocyanin, weight loss



51

FS-P-001

Some factors affected on betta fish (Betta splendens) breeding in

winter season

Panarat Phadee *




Abstract: Betta fish is a popular ornamental fish culture in Thailand. However,

Betta production is normally decreased in winter season because farmers could

not breed it according to some unsuitable environments for reproductive cell

development such as temperature, humidity and light. This study investigated 3

environmental factors on Betta breeding including outdoor situation (T1), the

automatic temperature control room at 30ºC (T2) and automatic temperature and

humidity control room 30ºC and 70% (T3), respectively. The 20 pairs of

broodstocks were raised in 3 conditions, and each condition was divided into 10

pairs for aeration and other for non-aeration. Experimental fish were reared for

24 days, then paring the couple for 5 days for equilibration. All pairs were bred

to determine the feed intake, mating rate, hatching period and number of hatching

fry. The results found that water temperature during raising and breeding Betta

in 3 conditions were 29, 30 and 30ºC, respectively, and relative humidity were

53, 57 and 67%, respectively. Dissolved oxygen of water during experiment in

aeration and non-aeration were 4.53 and 7.58 ppm, respectively. Upon mating

rate, the broodstocks raised in T2 without aeration had the highest mating rate of

90%, followed by T2 with aeration, T3 without and with aeration at 80, 70 and

50%, respectively. Upon hatching period, the broodstocks raised in T2 without

aeration, T3 with and without aeration showed shorter period than T1 in average

of 66, 62.4, 42.67, 43.43, 38.57 and 36 h, respectively. Upon number of hatching

fry of 3 conditions, the average numbers of 213.33, 254, 340.37, 311.43, 236 and

296 fish were recorded respectively. Moreover, the broodstocks raised in T3

showed higher feed intake than T1. The results indicate that increasing of

temperature and humidity could develop reproductive capacity and feed intake

of Betta in winter season.

Keywords: Betta fish, automatic temperature control system, automatic

temperature and humidity control system, environmental factors, winter breeding



52

BT-P-001

Antioxidant potential of rice grain processed by solid state

cultivation with Cordycep militaris

Sudarat Tasoon 1 and Luchai Butkhup 2*

1 Master student, Department of Biotechnology, Faculty of Technology,

Mahasarakham University, Mahasarakham 44150, Thailand 2 Natural Antioxidant Innovation Research Unit (NAIRU), Department of

Biotechnology, Faculty of Technology, Mahasarakham University,

Mahasarakham 44150, Thailand


Abstract: Rice grain (Oryza sativa L) was used as the raw material for Cordycep

militaris cultivation to investigate the effects of extract condition on phenolic

content and antioxidant activities. The 15% of ethanol concentration exhibited

most effective extraction of phenolic compounds from rice grain processed by

solid state cultivation with C. militaris. The ethanolic extracted showed the

highest phenolic content (1636.53±67.98 mg GAE/100g DW) and reducing

ability (FRAP, 1553.41±33.24 mg Fe2+/100g DW) and also high scavenging

activity more than 75%. The ethanolic extracts obtained from the rice grain

processed by solid state cultivation with C. militaris might be a potential

antioxidant supplement for application in food products.

Keywords: Cordycep militaris, antioxidant activity, ethanolic extracted, rice grain



53

BT-P-002

Optimization of microwave-assisted extraction of mulberry twigs

(Morus alba Linn.) on antityrosinase and antioxidant potential using

response surface methodology

Waranya Kanyaprasit 1 and Luchai Butkhup 2*

1 Master student, Department of Biotechnology, Faculty of Technology,

Mahasarakham University, Mahasarakham 44150, Thailand 2 Natural Antioxidant Innovation Research Unit (NAIRU), Department of

Biotechnology, Faculty of Technology, Mahasarakham University,

Mahasarakham 44150, Thailand


Abstract: Response surface methodology (RSM) has been used to optimize the

extraction conditions of bioactive components with relatively high antityrosinase

and antioxidant activity from mulberry twigs by using microwave-assisted

extraction (MAE). The results showed that the highest antityrosinase (2.51 mg

VE/g dw) and antioxidant activity (79.03 %scavenging and 250.03 mg

VEAC/100 g dw for DPPH assay, and 1342.75 mg Fe(II)/100g dw for FRAP

assay) were obtained with an extraction time of 5 min, 45% ethanol, and 70 ml/g

liquid to solid ratio. In this study, MAE can be used as an alternative to

conventional immersion extraction with respect to the recovery of bioactive

compounds from mulberry twigs, with the advantages of shorter extraction time

and reduced solvent consumption.

Keywords: mulberry twigs, microwave-assisted extraction, antityrosinase

activity, antioxidant activity


54

BT-P-003

Cold plasma effect on mustard green microgreens in different

growth periods

Vijitra Luang-In 1*, Worachot Saengha 1, Theerayut Bubpamala 1,

Piyatida Kitkayun 1 and Khanit Matra 2

1 Natural Antioxidant Innovation Research Unit, Department of Biotechnology,

Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai,

Mahasarakham 44150, Thailand 2 Department of Electrical Engineering, Faculty of Engineering,

Srinakharinwirot University, Nakhon Nayok 26120, Thailand


Abstract: Mustard green (Brassica juncea L. Czern and Coss) microgreens at 7

days were reported to contain higher bioactive compounds when seeds were

treated with cold plasma at 19 kV for 5 min in comparison with those from

untreated seeds. The aim of this work was to study cold plasma effect on mustard

green microgreens in different growth periods (14, 21 and 28 days) after seed

treated with cold plasma at 19 kV for 5 min. The results showed that seeds from

cold plasma treatment seemed to absorb water better than control. Consequently,

cold plasma significantly increased fresh weights at 14 and 28 days when

compared to the control; however, cold plasma effect led to lower stem lengths.

No difference in dry weights was observed. Thus, cold plasma can help farmers

gain higher fresh weight of mustard green microgreens.

Keywords: cold plasma, mustard green, microgreen, weight, seed


55

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