Hydrotherm  Fat  Determination  Study  1 HYDROTHERM  (WEIBULL  –  STOLDT  METHOD)  VS.  TRADITIONAL  AOAC  

922.06  

• Hydrotherm  meets/exceeds  the  requirements  of  AOAC  method  922.06  • Hydrotherm  advantages:  

Improved  operational  efficiency  -­‐  time  savings  of  nearly  7  minutes  per  sample  tested   Cost  Reduction  of  22%  per  sample  tested   Safer  work  environment  –  reduced  risk  of  lost  time  incident  

2 UNDERSTANDING  FAT  • What  is  Fat?  

Triglycerides  –  organic  compounds  free  of  nitrogen   Metabolic  products  found  in  plants  and  animals   Like  proteins  and  carbohydrates,  it  is  a  nutrient  used  as  an  energy  source  by  the  body   Has  a  caloric  value…1g  of  fat  =  9  calories  or  approximately  38  kilojoules   Often  referred  to  as  a  lipid,  it  is  insoluble  in  water,  but  soluble  in  organic  solvents  

• Fat  is  a  key  component  of  the  foods  we  eat   Nutrition   Appearance   Texture   Flavor  

• Fat  extraction  process   Extractable  fat  is  called,  “free  fat”   Fat  that  is  bound  or  enclosed  by  other  organic  components  cannot  be  directly  extracted   To  determine  total  fat  content  the  sample  must  be  digested  prior  to  extraction  

The  US  Nutrition  labeling  and  Education  Act  of  1990  (NLEA)  has  defined  total  fat  as  the  sum  of  all  fatty  acids  obtained  from  a  total  lipid  extract  expressed  at  triglycerides    

 

 

• Need  for  fat  extraction   FDA  mandate  for  food  nutritional  value  label   Mandatory  label  information  

Total  Fat,  Saturated  Fat,  Trans  Fat,  Cholesterol   Sodium,  Calcium,  Iron   Vitamin  A,  Vitamin  C   Total  Carbohydrates   Dietary  Fiber     Sugars   Protein  Total  Calories,  Calories  from  Fats  

Determination  of  “Good”  and  “Bad”  fat    

• Calculating  the  correct  total  fat  content   Sum  parameter  defined  by  the  selected  test  method   Determined  using  the  sample  matrix   Can  be  further  characterized  based  on  fatty  acid  spectrum  

3 STUDY  BACKGROUND  • Cooperative  evaluation  between  Lab  Synergy  and  Tyson  Foods  

Evaluation  of  acid  hydrolysis   Test  Methods  used  

Traditional  AOAC  922.06  method   Hydrotherm  Weibull-­‐Stoldt  method  

Food  and  Feed  products  with  various  mixed  matrices  tested   Over  1000  data  points  collected  

• Standard  AOAC  922.06  Acid  Hydrolysis  Test  Method  1.) 2  g.  samples  to  50  mL  beaker…add  10  mL  HCI…mix  well,  place  in  hot  water  bath  for  35  

minutes…add  10  mL  alcohol  and  cool.  2.) Transfer  aforementioned  mixture  to  Mojonnier  fat-­‐extraction  apparatus.    Add  in  3  portions  

to  flask  with  stopper,  shake  vigorously...add  25  mL  redistilled  petroleum  ether  and  again  shake  vigorously…let  stand  until  upper  liquid  is  practically  clear,  or  centrifuge  20  minutes  at  ca  600  ppm.  

3.) Draw  off  ether-­‐fat  solution  through  filter  into  125  mL  beaker-­‐flask  containing  porcelain  chips  or  broken  glass  

4.) Re-­‐extract  liquid  remaining  in  tube  twice,  each  time  with  only  15  mL  of  each  ether...shake  well  on  addition  of  each  ether…draw  off  clear  ether  solutions  through  filter  into  same  flask  as  before…evaporate  either  slowly  on  steam  bath…dry  fat  in  oven  at  100°C  to  constant  weight…remove  flask  and  counterpoise  from  oven,  let  stand  in  air  to  contain  weight,  and  weigh…correct  this  weight  by  blank  determination  on  reagents  used…report  as  %  fat  by  acid  hydrolysis.  

 

 

 

 

• Weibull-­‐Stoldt  Method  

1.)    Hydrolysis  of  sample  with  HCI  using  the  Hydrotherm  closed  loop  automated  system  

Filtration   Drying  of  filter              

2.) Extraction  of  fats  from  filtration  residue  by  use  of  a  single  organic  solvent  –  performed  using  the  Soxtherm  closed  loop  automated  extraction  system    

Evaporation  of  the  solvent   Drying  of  the  fat  

3.) Weighing  the  fat  4.) Summary/Benefits  

  Repeatable   Precise  results   Universal  use   High  through-­‐put   Improved  operating  efficiency   Lower  cost   No  fume  hood  required  

 • Hydrotherm  (Weibull-­‐Stold)  vs.  AOAC  922.06  Comparative  Data  

Stats   Hydrotherm  Observations    

Overall  Testing   Cost  Savings   Time  Savings   Safety  Advantages  

Conclusion