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HydrocortisoneCat. No.: B1951

CAS No.: 50-23-7

Formula: C21H30O5

M.Wt: 362.46

Synonyms:

Target: GPCR/G protein

Pathway: Glucocorticoid Receptor

Storage: Store at -20°C

In Vitro

13.3mg/mL in DMSO

Preparing

Stock Solutions

Mass

1mg 5mg 10mgSolvent

Concentration

1 mM 2.7589 mL 13.7946 mL 27.5893 mL

5 mM 0.5518 mL 2.7589 mL 5.5179 mL

10 mM 0.2759 mL 1.3795 mL 2.7589 mL

Please refer to the solubility information to select the appropriate solvent.

Shortsummary steroid hormone or glucocorticoid

IC₅₀ & Target

In Vitro

Cell Viability Assay

Cell Line: human lung microvascular endothelial cells

Preparation method The solubility of this compound in DMSO is >13.3mg/mL. General tips for

obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes

and/or shake it in the ultrasonic bath for a while. Stock solution can be stored

below -20°C for several months.

Reacting conditions: 4 or 6 μM; 16 h

Applications: In human lung microvascular endothelial cells, Hydrocortisone (HC) induced a

Product Name: HydrocortisoneRevision Date: 01/10/2020

Product Data Sheet

Solvent & Solubility

Biological Activity

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HydrocortisoneeeeeeeeeeeeCat. No.: B195555555555111111111

CAS No.: 505000500005050505055050505050-2323223232323232323233--------777777777777

Formula: C2CC2C2C2C2CCC2C2C2C2C2CC21H30O5

M.Wt: 362.46

Synonyms:

Target: GPCR/G protein

Pathway: Glucocorticoid Receptor

Storage: Store at -20°C

In Vitro

13.3mg/mL in DMSO

Preparing

Stock Solutions

Mass

1mg 5mg 10mgSolvent

Concentration

1 mM 2.7589 mL 13.7946 mL 27.5893 mL

5 mM 0.5518 mL 2.7589 mL 5.5179 mL

10 mM 0.2759 mL 1.37777777777959595959595955959595959 mmmmmmmmmmmLLLLLLLLLL 2.7589 mL

Pleaaaaaaaaasesesseseseseseseeeseeeeese rrrrrrrrrrefefefefefefefeffeeeeefeefe erererererererererer ttttttttttto oooooooooo ttthttt e solubility information to select the appropriate solventntnttnttttntt.

Shortsummary steroid hormone or glucocorticoid

IC₅₀ & Target

In Vitro

Cell Viability Assay

Cell Line:e:e:e:e:e:e:eee human lung microvascular endothelial cellslssslslslslslssss

PrPrPrPrrPrPrPrPrPrPP epepepepepepeepepepepepararaarararararara atataatattttatattttataatttatatiiiiiioioioiiioioioioioioioooooionnn n nnn nnnnnn method The solubility of this compound innnnnnnnnnnnnn DDDDDDDDDDDDDDMSMSMSMSMSMSMSMSMSMSMSSMMMMMMMMM O O O O O OO OO OO isisisisisisisisisisisis >13.3mg/mL. General tips for

obtaining a higher concentration: PlPlPlPlPlPlPlPlPlPPlPlPPleaeaeaeaeaeaeaeaeaaeaeaeaeaasesesessesesesesesssss warm the tube at 37°C for 10 minutes

and/or shake it in the ultrasonic bath for a while. Stock solution can be stored

below -20°C for several months.

Reacting conditions: 4 or 6 μM; 16 h

Applications: In human lung microvascular endothelial cells, Hydrocortisone (HC) induced a

Product Name: HydrocortisoneyRevision Date: 01/10/ 02020

etProduct Data Shee

Solvent & Soluuuuuubbbbbbiiiiiillllllllliiiittttttttyyyyyyyyyy

Biologicaaaallllll AAAAAAActivity

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concentration-dependent but biologically minor barrier enhancing and

protective effect. The combination of HC (4 μM) and ascorbic acid (VitC) (1000

μM) pre- or post-treatment significantly reversed the LPS-induced barrier

dysfunction.

In Vivo

Animal experiment

Animal models: 6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD) mouse

models

Dosage form: 0.4 mg/kg; intraperitoneal injection, 7 consecutive days

Applications: In Parkinson’s disease (PD) mouse models, Hydrocortisone caused

approximate two-fold increase of parkin expression in selected brain

subregions (striatum, ventral midbrain, and cerebellum), which was

responsible for cell survival against oxidative stress.. Hydrocortisone also

increased CREB expression. Hydrocortisone pretreatment markedly enhanced

dopaminergic neuronal survival against 6-OHDA intoxication.

Other notes: Please test the solubility of all compounds indoor, and the actual solubility may

slightly differ with the theoretical value. This is caused by an experimental

system error and it is normal.

See more customer validations on www.apexbt.com.

[1]. Barabutis N1, Khangoora V2, Marik PE2, et al. Hydrocortisone and Ascorbic Acid synergistically prevent and repair LPS- induced

pulmonary endothelial barrier dysfunction. Chest. 2017 Jul 21. pii: S0012-3692(17)31276-X.

[2]. Ham S1, Lee YI2,3, Jo M1, et al. Hydrocortisone-induced parkin prevents dopaminergic cell death via CREB pathway in

Parkinson's disease model. Sci Rep. 2017 Apr 3;7(1):525.

FOR RESEARCH PURPOSES ONLY.NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.Specific storage and handling information for each product is indicated on the product datasheet. Most APExBIO products are stable

under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage

temperature. Shortterm storage of many products are stable in the short-term at temperatures that differ from that required for

long-term storage. We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt

of the product, follow the storage recommendations on the product data sheet.

Product Citations

References

Caution

2 | www.apeexxxxxxxxxxxxxxxxbtbtbtbbtbbbbbbbbbbbbtt.c.c.comomomomomm

concentration-dependent but biologically minor barrier enhancing and

protective effect. The combination of HC (4 μM) and ascorbic acid (VitC) (1000

μM) pre- or post-treatment significantly reversed the LPS-induced barrier

dysfunction.

In Vivo

Animal experimememememememeemement

Animal modododddddododddeleleleleeeeeele s:s:s:s:s:s:ss:s:ss: 6-hydroxydopamine (6-OHDA)-induced Parkinsososososososososoossos n’nn’n’n’n’n’n’n’n’n’sssssssssss dididididididididididisesesesesesesesesessssss ase (PD) mouse

models

DoDoDooDooDoooDDDDDDDDDDDD sasasasasasasasasasaagegegegegegeggegegegg form: 0.4 mg/kg; intraperitoneal injection,,,,,,,,,,,,, 7 7 7 7 7777 7 7 777777 cocococococooococooooococococococc nsnsnsnsnsnsnsnsnsnsececececececececececececce uttive days

AAAAAAApAAAAA plications: In Parkinson’s disease (PD) momomomomomomomomomoomoomouusuuuuuuu e models, Hydrocortisone caused

approximate two-fold increase of parkin expression in selected brain

subregions (striatum, ventral midbrain, and cerebellum), which was

responsible for cell survival against oxidative stress.. Hydrocortisone also

increased CREB expression. Hydrocortisone pretreatment markedly enhanced

dopaminergic neuronal survival against 6-OHDA intoxication.

Other notes: Please test the solubility of all compounds indoor, and thheeeeeeeeee acacacacacacacacaaacactual solubility may

slightly differ with the theoretical value. This issss caaaaaaaaaaaaaaaausususususususususussededededededededededee bbbbbbbbbbbbbyyy yyyy an experimental

system error and it is normal.

See more customer validations on www.apexbt.com.

[1]. Barabutis N1, Khaaaaaaaaangngngngngngngngngggoooooooooooooooooooorararararararaarararaa VVVVVVVVVVVVVV2,22222 Marik PE2, et al. Hydrocortisone and Ascorbic Acid synnnnnnnnnnnnerererererererererererererererere gigigigigigigigiggigiggiggigistststststtttststsstttststststts icicicicicicicicicicccalalalalaalalalalaalallylllllylylll prevent and repair LPS- induced

pulmonary endoththththththththththththhhheleleleleeleleleleleleee iaiaiaiaiaaiaiaiaiaiaaiaiaallllllllllll babababababababababababaab rrier dysfunction. Chest. 2017 Jul 21. pii: S0012-3692(17)31276666666666------XXXXXXXXXXXXXX.X

[2]. Ham S1, Lee YI2,3, Jo M1, et al. Hydrocortisone-induced parkin prevents dopaminergic cell death via CREB pathway in

Parkinson's disease model. Sci Rep. 2017 Apr 3;7(1):525.

FOR RESEARCH PURPOSES S S S S ONONONONONONONONOONONONNLYLYLYLYLYLYLYLYLYLYLLY...NOT FOR HUMAN, VETERERERERERERERERERERERRININININNINININININNNNNNNI ARARARARARARARARARARARRARARRARRRY Y YY Y Y Y Y YY YYY DDDIDDDDDDID AGNOSTIC OR THERAPEUTTIC USE.Specific storage and hhhhhhhhhhhananananananananannaa dldldldldldldlddldldd inininininininining g g g gg g g gg ggggg ininininininininnii formation for each product is indicatted on the product datatatatatatatatatatataatatasasaasasasasasaasassasasassheheheheheheheheehehhehheeeeheeeh etetetetetetetetetetettetet. Most APExBEE IO ble products are stab

under the recommmmmmmmmmmmmmmmenenenenenenenenenenenenennddedededddedeeeeedeeeeddeededdded d dd d d ddddddddddd conditions. Products are sometimes shipped at a temperature that differs from the recommended storageed at a temperature that differs from the recommended storag

temperature. Shorttttttttttttett rm storage of many products are stable in the sshort-term at temperatures that differ from that required for

long-term storage. We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt e

of the product, follow the storage recommendations on the product ddata sheet.

Product Citations

References

Caution

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APExBIO Technologywww.apexbt.com

7505 Fannin street, Suite 410, Houston, TX 77054. Tel: +1-832-696-8203 | Fax: +1-832-641-3177 | Email: info@apexbt.com

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APExBIO Technologywww.apexbt.com

7505 Fannin street, Suite 410, Houston, TX 7705454545454545445454544454545454544.........Tel: +1-832-696-8203 | Fax: +1-832-641-3177 | Email: infoooooooooooooooo@a@a@a@a@a@a@a@a@a@a@a@a@a@a@a@a@a@@a@a@@@ pepepepepepepepepepeeeeepepeppepepppepepexbxbxbxbxbxbxbxbxxxbxxbx t.com