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http://www.humichealth.info/statements.html

Statements from ResearchersAbout the Anti-Viral Efficacy of Humic Acid*HumicAcid.info Editor's Note:The term (Humic Acid) or (Humic Acids) has been

substituted in the following statements where theoriginal wording was in scientific terms that detailed

chemical compositions of Humic Acid.

1. HEMORRHAGIC FEVER VIRUSES"As shown, the (Humic Acids) exhibited substantial efficacyagainst both hemorrhagic fever viruses.natural-product HA

(Humic Acid) particularly so against Punta Toro A virus."National Institutes of Allergy and Infectious Diseases(NIAID), under the auspices of the National Institutes ofHealth, August 2001 - January 2002

2. HERPES SIMPLEX VIRUS TYPES IAND II"It is therefore concluded that it is possible to test the anti-

viral effects of all four (Humic Acid) compounds up to 500ug/mL without any drug-related cytotoxicity." "Furthermore,three series of experiments were designed to explore thepotential mechanisms of action. The first series ofexperiments demonstrated that all four compounds (ofHumic Acid) can inactivate both HSV-1 and HSV-2 infections

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with different anti-viral activities. The second series of

experiments demonstrated that the compounds (of HumicAcid) can protect cells against HSV-1 and HSV-2 infection.The third series of experiments demonstrated that the

compounds (of Humic Acid) can interfere with theproliferation of HSV-1 and HSV-2 after the virus enters thecells."

Meeta Patnaik , M.D., Senior Research ScientistHun-Chi Lin, PhD, Director, Clinical TrialsSpecialty Laboratories, Santa Monica, CA, August 1998

3. HIV-1

the ability to inhibit the replication of wild-type strains (ofHIV-1) is an obvious (and, arguably, the most important)prerequisite in order that any HIV-1 drug be considereduseful. The results obtained to date have shown that (natural)Humic Acid as well as synthetic humic acid(s) do, in fact,inhibit the replication of HIV-1 wild-type strains, in addition toassaulting in a unique way the intracellular replicative eventsof the virus.

It was found in this work that both synthetic as well asnaturally-occurring humic substances extracted from soil arein fact capable of inhibiting HIV-1 replication.Research team at USC School of Medicine, January, 1998.

The Humates are potent inhibitors of acute HIV infection inantiviral evaluation models. The present studies point to anantiviral target occurring before proviral integration, possiblyinvolving virus attachment and entry. We were able to

confirm antiviral activity for the Humates and define theirpotential antiviral target to the pre-integration phase of HIVreplication, providing preliminary data that a portion of theantiviral activity is due to inhibition of virus attachment.

Jim A. Turpin Ph.D., Manager Retrovirus ResearchLaboratory

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Tracy Loftus, Supervisor Retrovirus Research LaboratorySouthern Research Institute, September, 2000

(Humic Acid) inhibited HIV-1 infection of MT-2 cells

with an IC(50) of 12.5 microg/ml. Treatment of freeand cell-attached HIV with (Humic Acid) irreversiblyreduced infectivity, while the susceptibility of targetcells to the virus was not impaired by treatment priorto infection. The infectivity of the HIV particles wasinhibited by interference with CD4 binding and the V3

loop-mediated step of virus entry. No viralresistance to (Humic Acid) developed over a 12-week period in vitro. (Humic Acid) therefore holdspromise for the treatment of HIV-infected patients.van Rensburg CE, Department of Pharmacology,University of Pretoria, Pretoria, South Africa; DekkerJ, Enerkom (Pty) Ltd, Pretoria, South Africa; Weis R,Smith TL, Janse van Rensburg E, Schneider J.

Humic acids are natural constituents of soil and ground

waterand mainly consist of mixtures of polycyclic phenoliccompounds. (Humic Acid) inhibited HIV-1 infection of MT-2cells with an IC50 of 50-300 ng/ml. Inhibition of HIV-inducedsyncytium formation was observed at 10-50 micrograms/ml.Treatment of free and cell-attached HIV with (Humic Acid)irreversibly reduced its infectivity. (Humic Acid) interferedwith the CD4-induced proteolytic cleavage of the V3 loop ofvirion gp120SU. Furthermore, binding of V3 loop-specificantibodies was irreversibly inhibited, whereas binding ofsoluble CD4 to gp120SU on virus and infected cells was notaffected. In conclusion, our data suggest, that the (HumicAcid) inhibits the infectivity of HIV particles by interferencewith a V3 loop-mediated step of virus entry.Schneider J, Weis R, Manner C, Kary B, Werner A, SeubertBJ, Riede UN.

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Abteilung Virologie, Institut fur Medizinische Mikrobiologieund Hygiene der Universitat, Freiburg, Germany.

The anti-HIV-1 activity of the new humic acid derivative hasbeen determined in vitro, and the serial passages at lowconcentrations have been performed to investigate theemergence of resistance to this drug candidate.Our investigations wereperformed to find perspectivecompounds and their combinations to employ them intopical microbicides which possess the ability to block theHIV transmission on different stages of virus cell interaction.We have studied original compounds from different groups:

sulfated chitosanes, humic acids and plant polyphenolderivatives.The ED50 values of the humic acid derivatives against HIV-1laboratory strain and HIV-1 M-tropic AZT-resistant wild-typestrain were 0.85 and 3.5 mg/ml respectively, withoutappearance of any detectible resistance.The new humic acid derivative is an effective inhibitor ofvarious HIV strains. According to our data, this compounddisorders HIV replication and yields defective un-infectiousvirions.

Edward Karamov PhD, Director of Immunochemistry Group,Ivanovsky Inst. O f Virology, Moscow, Russia

4. INFLUENZA VIRUSES"natural-product humate(s) (Humic Acid) were found to bequite effective against all three influenza viruses."

National Institutes of Allergy and Infectious Diseases(NIAID), under the auspices of the National Institutes ofHealth, August 2001 - January 2002

Humic Acid inhibits the in-vitro replication of influenza virusA/WSN/33 (H1N1) in Madin-Darby canine kidney (MDCK) cellsat concentrations of no cytotoxicity. The IC50 for Humic Acid

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was 48.61 +/- 7.32 microg/ml and 55.27 +/- 5.46 microg/mlrespectively when the compound was added at the stage ofviral adsorption or post-adsorption. Humic acid inhibits theendonuclease activity of viral RNA polymerase.

Lu FJ, Tseng SN, Li ML, Shih SR.Department of Biochemistry, College of Medicine, NationalTaiwan University, Taipei.

5. TOXICITY"For each sample, three concentrations of test article (HumicAcid) were prepared and each injected into the tail veins of10 Swiss Webster mice at dose levels of 50 mg/kg of bodyweight, 25 mg/kg, and 12.5 mg/kg the mice were examinedfor symptoms of toxicity for 14 days. All animals survived thetest, and in my opinion, the clinical observations made werenot indicative of toxicity."Richard Schlesinger, Ph.D., D.A.B.F.T.BioScreen Testing Services, Inc., Torrance, CA, August 1998

"All (Humic Acids) evaluated were not cytotoxic at levels atleast as high as 100 mg/mL"National Institutes of Allergy and Infectious Diseases

(NIAID), under the auspices of the National Institutes ofHealth, August 2001 - January 2002

DISCLAIMER: This website presents a collection of statements from around the worldabout the benefits of Humic Acid. This information is provided for informational purposesonly. These statements were variously made over several decades of time. There are many

sources of Humic Acid around the globe, and they differ significantly in their physical andchemical properties. This website does not intend to provide medical advice, nor does itintend to suggest that all Humic Acid preparations will be of equal benefit. Nothing herein isintended to be an endorsement of or a solicitation to purchase any particular Humic Acid

preparation. The FDA has not evaluated any statement made on this website. Theinformation herein is not intended to diagnose any disease, nor is it intended to prescribeany preparation that claims to diagnose, treat, cure or prevent any disease.

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