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Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Introduction to the Introduction to the methodology of EEG methodology of EEG
recordingrecordingFriday October 20th
2006
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Summary Summary
• A. Generators and modulators of EEG signal• B. Understanding instrumentation• C. Main ethical issues • D. Physiological and electronical sources of noise• E. Constraints for experimental protocols in EEG• F. Configuration of EEG recording system and selection of montage• G. Management of connectics• H. Paste-up and problem solving during the recording session• I. Digitization• J. Cleaning/decontamination of sensitive equipment / equipment
maintenance
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
A. Generators and modulators of EEG A. Generators and modulators of EEG signalsignal
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Generators of EEG signal Generators of EEG signal
• When reached by an input, the presynaptic neuron:– Releases some
neurotransmitter in the synaptic cleft
• The dendritic process of the post-synaptic neuron:– shows a local change in its
membrane’s permeability– generates a primary
(intracellular) current from the locus of the synapse to the soma
– Generates a secondary/return extracellular current to close the loop
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Generators of EEG signal Generators of EEG signal
• Cortical pyramidal neurons, arranged in layers– The movement of the ions is
creating an open field (no cancellation)
• When a local community of tens of thousands of neurons are activated simultaneously by some input, a signal can be detected as far as at the surface of the scalp
• This signal is EXTREMELY tiny, and requires many precautions when measured
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Generators of EEG signal Generators of EEG signal
• And the miracle occurs
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Modulators Modulators
• Age– Children:
• Changes in frequency content due to the size of the loops in the anatomical networks
• Changes in the conduction time due to myelinization• Change in the amplitude of the signal due to myelinization
– Adult• Increased variability over 40
• Vigilance – Chronopsychology (more details next)– Drugs– Caffeine
• Body temperature• Hormonal cycles (women)• Laterality
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Modulators Modulators
• Circadian rhythms – Global power is maximal during
the afternoon– Theta power has two peaks at
4pm and midnight– Induced alpha is maximal in the
afternoon– Beta is maximal between 5pm
and 7pm
• The modulation is dependent on the location of the electrodes
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
B. Understanding instrumentationB. Understanding instrumentation
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Overview Overview
• Junction skin-electrode• Analog conduction• Differential amplifiers• ADC
• Integration of triggers
• Transfer to the CPU / storage
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Transduction Transduction
• The living tissues contain free ions
• The wire is conveying electrons
• The transfer of the signal from one material to the other requires a chemical transformation
• Oxidation or reduction (AC)
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Transduction Transduction
• Eg. Ag / AgCl electrode:– OXIDATION
• If an electron moves from the wirewire to the electrodeelectrode toward the conductive gelconductive gel:
– It reacts with AgCl » ee-- + AgClAgCl -> AgAg + ClCl--
– Cl- becomes hydrated and enters the conductive paste
– REDUCTION• If ion moves from the conductive gel to
the electrode:– It reacts with solid Ag
» AgAg + ClCl-- -> ee-- + AgCl AgCl – AgCl becomes insoluble– one electron is liberated to the wire
• REVERSIBLE
e-e-
e-e-
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Transduction Transduction
• Eg. Ag / AgCl electrode:
• The Ag/AgCl electrode is non-polarizable (or minimally polarizable)
• POLARIZATION– The anion (Cation) is unable to move
freely across the gel/electrode border– The concentration of ions at the border is
altered.– Ions concentrate over the border with the
electrode and create a steady potential (bi-layer, capacitance)
– This steady potential hampers the movement of the charges
• This is important since the biopotential we intend to measure is in the range of 1/1000 of the half-cell potential (local potential at the junction between the conductive paste and the electrode)
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Analog conduction Analog conduction
• As soon as the potentials are digital, they are immune to noise (not to deletion)
• Between the cap and the ADC, the minuscule currents are traveling through the cables and in the amplifier.– Contamination through movements of the cables – Contamination by cross-talk inside the amplifier
and at the multiplexer of the ADC
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Differential amplification Differential amplification
• We amplified to push the deflection of the pens (mechanical)
• We amplify to bring the signal in the range of the ADC (usually 0-1 to 0-5 V)
• But more importantly, the purpose is differential amplification…
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Differential amplification Differential amplification
• Principle of differential amplification: the CMR – (Signal + noise) – (noise)
• Take a scalp electrode (say C3) and a fixed point (GND)
• Measure one potential difference
• Take a reference electrode (say MA1) and a fixed point
• Measure a second potential difference
• (Signal + noise) – (noise) = “a very clean” signal
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Differential amplification Differential amplification
• The ability of the amplifier to reject the common mode noise is called the CMRR
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Differential amplification Differential amplification
• Amplifier Input impedance– Separate the differential
input with a high resistance
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Analog-to-Digital Analog-to-Digital ConversionConversion
• Sampling frequency: Nyquist and aliasing
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Analog-to-Digital Analog-to-Digital ConversionConversion
• Sampling frequency • ADC range• Quantization
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Acquisition and storageAcquisition and storage
• Data acquisition and storage– Reasonable sampling rate– Backup
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Understanding Understanding instrumentationinstrumentation
• Quikcap • Headbox• Power unit• System unit
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
C. Main ethical issues C. Main ethical issues
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Electrical safetyElectrical safety
• Security for the subject and security for the equipment– Faulty connections – Additional devices (response pads, sensors)– Ground loops– Static discharges– Chassis leakage– EMI in crossing wires
• Isolation amplifiers (Neuroscan system) are regulated by IEC 601-1 specifications.
• Additional devices connected to Neuroscan have to be detailed in the application to the EEG committee
• Order to plug or unplug the components
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Infection riskInfection risk
• Most of the supplies, especially those in contact with the subject (eg. needles), are disposable
• Any supply in contact with the subject does not return to the main. – eg. the gel is sampled in a cup. Do NEVERNEVER refill a syringe in the main container.
• Moderate skin preparation: a subject should never be bleeding as a result of skin preparation.
– Inspection for the presence of blood after experiment (to choose the decontamination procedure)
• Decontamination of non-disposable equipment
• Is regulated by [American Electroencephalographic Society. Report of the Committee on Infectious Diseases. J Clin Neurophysiol 1994;11:128-132.].
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Infection riskInfection risk
Object & Classification Use of Item Decontamination required
after cleaning
Critical Enters vascular system or sterile
body tissues
Sterilization and holding in sterilized state. High level disinfection is not sufficient
Semi-Critical Comes in contact with non-intact skin or intact mucous membranes
High level disinfection (by heat or chemicals)
Non-Critical Comes in contact with
intact skin
Intermediate or low level disinfection
Spaulding's classification of devices/medical instruments
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
D. Physiological and electronical sources D. Physiological and electronical sources of noiseof noise
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• Physiological artifact– Ocular domain– Muscular domain– EKG– Respiratory– Movement – EDR/sweating
• Subjects’ instruction and online monitoring• Instrumental noise
– EMI : wireless or line noise (60 Hz)– Sway of the cable– Electrodes poorly attached (pop)– Electrode noise– Amplifier noise – Flicker noise (DC recordings!)– Amplifier blocking
• Shielding and guarding
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• Artifacts from the ocular domain
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• With proper alignment of EOG electrodes, horizontal EOG do not pick up the signal from vertical eye movements
1 s
GOOD BAD
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• Saccade / eye movements
1 s
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• Muscles
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• How life could be easy without muscles
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• (and with enough time to average thousands)
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
– EKG
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
– Respiratory
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
– Movement
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
– EDR/sweating
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• Physiological artifact– Ocular domain– Muscular domain– EKG– Respiratory– Movement – EDR/sweating
• Subjects’ instruction and online monitoring• Instrumental noise
– Flicker noise (DC recordings!)– EMI : wireless or line noise– electrode noise– amplifier noise – Sway of the cable– Electrodes poorly attached (pop)– Amplifier blocking
• Shielding and guarding
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• A cell phone
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• Poor contact / Electrode pop
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Interferences Interferences
• 60 Hz
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
E. Constraints for experimental protocols E. Constraints for experimental protocols in EEGin EEG
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Protocols Protocols
• Paradigms– Evoked response– Steady-state paradigms
• A single source of variation between conditions “All other things being equal”
• A good Stimulation/recording coupling “time accuracy in analog and digital stimuli/triggers”
• Subject screening– Day-before instruction– Accepting or rejecting a volunteer – artifacts instruction, task instructions, – Online monitoring of data quality and management of breaks
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
F. Configuration of EEG recording F. Configuration of EEG recording system and selection of montagesystem and selection of montage
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Configuration Configuration
• Configuration of data recorder (scan-acquire mode)– Sampling frequency– DC/AC recording (DC and EDR resident on the skin; DC
and choice of electrodes)– Triggers
• Selection of montage– Only referential recording– Reference electrodes– Ground electrode
• Ancillary recording (EOG, surface EMG, EKG)
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Montage 10 percentMontage 10 percent
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Montage equidistant (eg. Montage equidistant (eg. EGI)EGI)
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Montage 128 NSLMontage 128 NSL
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Montage referenceMontage reference
• Choice of the reference electrode– Cephalic/non cephalic– Well-attached – Single electrode or pair of electrode– Pair physically or digitally linked
• Position of the ground– In midline for ERL
• Remontage
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Next session:Next session:practical sessionpractical session
G. Management of connecticsG. Management of connectics H. Paste-up and problem solving during the recording H. Paste-up and problem solving during the recording
session session I. Digitization I. Digitization
J. Cleaning/decontamination of sensitive equipment / J. Cleaning/decontamination of sensitive equipment / equipment maintenanceequipment maintenance
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
G. Management of connecticsG. Management of connectics
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Cables everywhereCables everywhere
• Main cables are present in continuous
• Cables being specific to an experiment are removed after each experiment
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
H. Paste-up and problem solving during H. Paste-up and problem solving during the recording sessionthe recording session
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
Transduction Transduction
Practical recommendation:– Do not mix electrodes’ metal– Do not mix gels– homogenous contact skin-gel-electrode
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
• Electrodes impedance– Poor impedance
• Is the electrode’s surface clean (eg did I perforate a crust into the electrode’s hole while injecting conductive gel?). Did I rub enough the scalp? Did the subject come with inadequate hair condition? Is the electrode filled enough?
– Infinite impedance
• Am I sure that there’s no damage to the wires?• Amplifier’s gain error• Poor signal on some electrodes
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
I. DigitizationI. Digitization
Human Brain and Behavior Laboratory
Center for Complex Systems and Brain Sciences
J. Cleaning/decontamination of J. Cleaning/decontamination of sensitive equipment / equipment sensitive equipment / equipment
maintenancemaintenance