Wetzel CV

Curriculum Vitae

Scott Allen WETZEL, Ph.D.

The University of MontanaDivision of Biological Sciences

Clapp Building, Room 21632 Campus Drive #4824

The University of MontanaMissoula, MT 59812

(406)243-2168scott.wetzel@umontana.edu

Employment History

6/17 – Present Scientific Director, University of Montana Electron Microscopy Core Facility, The University of Montana, Missoula, MT

05/13 – Present Associate Professor, Division of Biological Sciences, Integrative Microbiology and Biochemistry (IMB)/Cellular Molecular and Microbiology (CMMB) Graduate Program and Center For Environmental Health Sciences, Universityof Montana, Missoula, MT.

09-09 – Present Scientific Director, Molecular Histology and Fluorescence Imaging CoreFacility, University of Montana, Missoula, MT.

09/05 – 05/13 Assistant Professor, Division of Biological Sciences, Integrative Microbiology and Biochemistry (IMB)/Cellular Molecular and Microbiology (CMMB) Graduate Program and Center For Environmental Health Sciences, Universityof Montana, Missoula, MT.

12/01 – 08/05 Postdoctoral Fellow, Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR

9/94 – 12/01 Graduate Research Assistant, Department of Molecular Microbiology and Immunology, Oregon Health & ScienceUniversity, Portland, OR

10/91 - 9/94 Research Associate II, Division of Immunology, Beckman Research Institute ofCity of Hope National Medical Center, Duarte, CA

Educational History

2/02 – 8/05 Postdoctoral Fellow, Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, ORMentor: David C. Parker

9/94 – 2/02 Ph.D. in Immunology, Molecular Microbiology and Immunology Department, Oregon Health & Science University, Portland, OR

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Wetzel CV

Thesis: Imaging and analysis of the Immunological Synapse Formed Between CD4+ T lymphocytes and Antigen Presenting Cells. Mentor: David C. Parker

10/88 - 11/92 M.S. in Biology, Emphasis Microbiology and Immunology, California StatePolytechnic University, Pomona Thesis: Studies of the phagocytic capabilities of cultured epidermal Langerhans Cells and subsequent fungicidal activity after incubation with free and liposome encapsulated Amphotericin B. Mentors: Pamela Sperry and Jill Adler-Moore

9/83 - 5/87 B.A. in Biology, University of La Verne, La Verne, CA. Thesis: Mutagenic Potential of Common Chemicals using the Yeast Rho mutation as a readout. Mentor: Wendy Wooten

Teaching Experience

09/05 – Present Assistant/Associate Professor, Division of Biological Sciences and the Center for Environmental Health Sciences, The University of Montana, Missoula, MT.

Courses Taught: BIOB 410 – General Immunology Lecture – 3cr lecture-based course each fall semester. Covering all aspects of modern molecular Immunology. Developed curriculum and learning outcomes, delivered all lectures, designed exams and other grading metrics. Enrollment of 45 – 55 students.

BIOB 411 – Immunology Laboratory – 2 cr laboratory course taught each fall semester. Developed curriculum and learning outcomes, authored laboratory manual, directed Teaching Assistant. Enrollment 20 – 30

MICB 502 – Advanced Immunology. 3 cr Graduate course focusing on discussion of primary literature in cutting-edge immunology. Students wrote NRSA application and serve on a mock study section during the course. Taught Spring semester of even-numbered years.

BIOB 596 – Principles of Light Microscopy. 3 cr Graduate, lecture-based course discussing the fundamental concepts of current cutting-edge light microscopy techniques used in life science research. Also contains significant hands-on experience for the students using advanced imaging platforms available on the UM campus. Taught Spring semester of odd-numbered years.

BCH/BIOB/BIOM 594 Professional Seminar Series 1 cr. Graduate seminar course. Coordinated invitations for all seminar speakers, budgeting and making travel plans for outside speakers, coordinated visit schedules and assessed required graduate student speaker evaluations. Taught in Spring semester 2010 - 2014.

03/12, 10/13, 10/14 Guest Lecture, BIOB 191N: Human Genetics and Health,

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“Pesticide Exposure and Health” (Portion of the Global Learning Initiative) an Undergraduate Seminar Course on Genes and Human Health, University of Montana

9/17, 9/18 Guest Lecture, BIOB 291; Biology Research and Careers “Medical Microbiology Careers”

02/17-5/17 Co-Taught, PHAR 491 Basic Immunology (delivered 16 of 20 lectures)

02/12 Guest Lecture, Biomedical Sciences in the Headlines course at the Osher Life Long Learning Institute at UM (MOLLI), Vaccine Development

03/09 Guest Lecture, HS389 Recent Advances In Autoimmune Disorders And Women’s Health. Sjögren’s Syndrome. University of Montana College of Health Professions and Biomecial Sciences Women’s Health Continuing Medical Education Program

02/09 – Present Video Guest Lecture, Hubel Elementary School 3rd & 4th grade, Des Moines,

IA. Scientists as Expolorers and a research talk on the health effects of Atrazine exposure in drinking water.

6/02 to 07/11 Faculty Member, Annual International Course on 3D Microscopy of Living Cells, University of British Columbia, Vancouver, BC, Canada. Co-coordinate student laborotories, serve as group advisor to a student working group, present lectures on tissue culture techniques and concepts of student set labs.

6/02 to 07/11 Faculty Member, 3D Image Processing Course, University of British Columbia, Vancouver, BC, Canada. Assist students in hands-on analysis of imaging data, teach use of ImageJ software, present lecture on movie generation and features of QuickTime Pro software.

4/02 –6/03 Instructor, Portland Community College, Portland, OR

Course Taught: Introduction to Biotechnology lecture and associated lab course

Developed course curriculum within the broad guidelines presented in the PCC catalog, prepared and delivered all lectures, prepared laboratory materials, prepared comprehensive handouts on various aspects of biotechnology, wrote and administered exams to evaluate the student’s understanding of materials covered, evaluated term papers and student oral presentations, and assigned final grades.

09/97 – 05/05 Adjunct Immunology Instructor, Oregon Health Sciences University Clinical Laboratory Scientist Training Program. Taught lectures on adaptive and innate imunity, lymphocyte development, lymphocyte activation, immunity to pathogens, immunological techniques, and PCR.

09/00 – 09/04 Guest Lecturer, University of Portland, Portland, OR. Presented lectures on T cell signaling and T cell development.

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Publicationshttps://orcid.org/0000-0001-6843-4583

1. Jim Reed and Scott Wetzel. (2018) “CD4+ T cell Differentiation and Activation” in Immunotoxicity Testing: Methods and Protocols. Rockwell, DeWitt and Bowman editors. Springer Publishing. Peer-Reviewed Book Chapter. 1083:335-351. PMID:29882148

2. Thueson, L.E.; Emmons, T.R.; Browning, D.L.; Kreitinger, J.; Shepherd, D.; Wetzel, S.A. (2015) In vitro Exposure To The Herbicide Atrazine Inhibits T Cell Activation, Proliferation, And Cytokine Production And Significantly Increases The Frequency Of Foxp3+ Regulatory T Cells. Toxicological Sciences. 143:418-29. PMID: 25433234

3. Osborne, D.O. and Wetzel, S.A. (2012) Trogocytosis leads to sustained signaling in CD4+ T cells. Journal of Immunology. 189:4728-39 PMID: 23066151

4. Doherty, M.D.; Osborne, D.G.; Browning, D.L.; Parker D.C.; Wetzel, S.A. (2010) Anergic CD4+ T cells form mature immunological synapses with enhanced accumulation of c-Cbl and Cbl-b. Journal of Immunology. 184: 3598-3608 PMCID: PMC174818

5. Thauland, T.J.; Koguchi, Y.; Varma, R.; Wetzel, S.A.; Dustin, M.L.; Parker, D.C. (2008) Immunological Synapse Formation by T Cell Subsets: TH2 Cells Form Multifocal Synapses. Journal of Immunology. 181:393 PMCID: PMC2441916

6. Blake, D.J.; Wetzel, S.A.; Pfau, J.C. (2008) Autoantibodies from mice exposed to Libby amphibole asbestos binds to SSA/Ro52-enrigched apoptotic blebs of murine macrophages. Toxicology. 246:172 PMCID: PMC2346587

7. Wetzel, S.A. and Parker D.C. (2006) MHC Transfer from APC to T Cells Following Antigen Recognition. Critical Reviews in Immunology. 26(1):1-21 PMID: 16472066

8. Dillon, T.J.; Carey, K.D.; Wetzel, S.A.; Parker, D.C.; Stork, P.J.S. (2005). Regulation of the small GTPase Rap1 and ERKs by the costimulatory molecule CTLA-4. Molecular Cell Biology. 25:4117 PMCID: PMC1087740

9. Wetzel, S.A.; McKeithan, T.W.; Parker D.C. (2005) Peptide-Specific Intercellular Transfer of MHC Class II to CD4+ T Cells Directly From the Immunological Synapse Upon Cellular Dissociation. Journal of Immunology 174(1):80-89 PMID: 15611230

10. Dillon, T.J., Karpitski, V., Wetzel, S.A., Parker, D.C., Shaw, A.S, Stork, P.J.S. (2003). Ectopic B-Raf expression enhances extracellular signal-regulated kinase (ERK) Signaling in T cells and prevents antigen presenting cell-induced anergy. J. Biol. Chem., 278:35940. 4117 PMID: 12855697

11. Wetzel, S.A.; McKeithan, T.W.; Parker D.C. (2002) Live Cell Dynamics and the Role of Costimulation in Immunological Synapse Formation. Journal of Immunology. 169(11): 6092 PMID: 16472066

12. Wetzel, S.A. (2001) Imaging and analysis of the immunological synapse formed between CD4+ T Lymphocytes and antigen presenting cells. Ph.D. Thesis, Oregon Health Sciences University

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Wetzel CV

13. Sperry P.J.; Cua D.J.; Wetzel S.A.; Adler-Moore, J.A. (1998) Antimicrobial activity of AmBisome and non-liposomal Amphotericin B following uptake of Candida glabrata by murine epidermal Langerhans cells. Medical Mycology 36(3):135 – 141 PMID: 9776826

14. Primus, F.J.; Finch, M.D.; Wetzel, S.A.; Masci, A.M.; Schlom, J.; Kashmiri, S.V.S. (1994) Monoclonal Antibody Gene Transfer: Implications for Tumor - Specific Cell - Mediated Cytotoxicity. Annals of the New York Academy of Science. 716: 154 – 166 PMID: 8024192

Invited Presentations

5/16/16 Trogocytosis-mediated signaling drives GATA-3 and IL-4 Expression in CD4+ T cells. Annual Meeting of the American Association of Immunologists in Seattle, WA.

3/24/14 “Post Trogocytosis: Why Do Hungry T Cells Take A Bite Out of Antigen Presenting Cells?” Johns Hopkins University Immunology Training Program, Baltimore, MD.

12/06/12 “T Cell Autonomous Signaling As A Result Of Trogocytosis” Rocky Mountain Laboratories Immunology Seminar, NIAID, NIH, Hamilton, MT. (

5/18/12 “Trogocytosis: Hungry T Cells Take A Bite Out Of APC”Biological Sciences Department, California State Polytechnic University, Pomona, CA.

4/4/12 “Trogocytosis: Why Hungry T Cells Take A Bite Out Of APC” Biology Department, Wellesley College, Wellesley, MA.

10/20/10 “Image Colocalization Analysis”. University of Montana MHFI Core Facility Technology Seminar

10/12/09 “Immunosuppression Associated With Atrazine Exposure.”Integrative Microbiology and Biochemistry Seminar, University of Montana

5/1/06 “Trogocytosis: Why Do Hungry T Cells Capture APC Membrane Components From The Immunological Synapse?” Integrative Microbiology and Biochemistry Seminar, University of Montana

3/3/06 “Immunological Synapses Formed By Anergic T Cells”. University of Montana Immunology Research Core Group Seminar

7/22/04 “Characterization of Immunological Synapses formed by Anergic T cells. 12th International Congress of Immunology, Montreal, Canada

3/8/03 Peptide-Specific Transfer of MHC:peptide Complexes From Antigen Presenting Cells to T Cells Directly Through the Immunological Synapse. Keystone Symposium on Cell Biology of Antigen Processing and Presentation. Keystone, CO

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Wetzel CV

11/5/02 Formation and Dynamics of the Immunological Synapse, Molecular Microbiology and Immunology Departmental Retreat, Oregon Health & Science University, Portland, OR

7/26/01 “The Role of Costimulation in the formation of the Immunological Synapse.” 11th International Congress of Immunology, Stockholm, Sweden

5/16/01 Image Acquisition and Analysis of the Immunological Synapse. Image Analysis Workshop, Oregon Health & Science University

Meeting Abstracts (* denotes undergrad researcher, ** graduate student researchers)

1. Dawit Mengistu* and Scott Wetzel. 2018. Studying the effects of Atrazine-induced estrogen on the development of regulatory T cells. University of Montana Undergraduate Research Conference.

2. Jim Reed** and Scott Wetzel. 2016. Trogocytosis-mediated signaling drives GATA-3 and IL-4 Expression in CD4+ T cells. Annual Meeting of the American Association of Immunologists in Seattle, WA.

3. Tiffany Emmons** and Scott Wetzel. 2014. The role of estrogen in atrazine-mediated inhibition of CD4+ T cell function and induction of CD4+CD25+Foxp3+ regulatory T cells.Annual Meeting of the American Association of Immunologists in Pittsburgh, PA.

4. Naomi Crane*, Joanna Kreitinger*, Timmon Hayes*, Scott Wetzel. 2014. Trogocytosis efficiency is dependent upon TCR affinity for MHC: Peptide ligands.” Annual Meeting of the American Association of Immunologists in Pittsburgh, PA.

5. L.E. Thueson**, and S.A. Wetzel. 2011. In vitro exposure to the common water contaminant Atrazine inhibits CD4+ T cell proliferation and increases the frequency of FoxP3+ cells. Immunology 2011: Annual Meeting of the American Association of Immunologists. San Francisco, CA.

6. T.L. Hayes* and S.A. Wetzel. 2010. Trogocytosis Efficiency is Dependent Upon TCR Affinity for MHC:peptide Ligands. National Conference on Undergraduate Research, Missoula, MT.

7. D.G. Osborne**, Thueson, L.E. ** and S.A. Wetzel. 2010. Trogocytosis leads to sustained signaling within CD4+ T cells. August 2010. Intermountain West Immunology Working Group Meeting, Rocky Mountain Laboratories, Hamilton, MT.

8. D.L. Browning*, S.A. Wetzel. 2009 In Vitro Exposure to Atrazine Does Not Alter Activation, But Prevents Proliferation of Primary T Cells. Western Region COBRE-INBRE Scientific Conference. Big Sky, MT.

9. D.G. Osborne**, Thueson, L.E. ** and S.A. Wetzel. 2009. Trogocytosis leads to sustained signaling and selective survival of CD4+ T cells. Western Region COBRE-INBRE Scientific Conference. Big Sky, MT.

10. S.A. Wetzel and D.L. Browning*. In vitro exposure to Atrazine does not alter activation, but prevents proliferation of primary murine B cells, T cells, or macrophages. Immunology 2009: Annual Meeting of the American Association of Immunologists. Seattle, WA..

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11. D.G. Osborne**, Thueson, L.E. ** and S.A. Wetzel. 2009. Trogocytosis leads to sustained signaling and selective survival of CD4+ T cells. Immunology 2009: Annual Meeting of the American Association of Immunologists. Seattle, WA.

12. S.A. Wetzel. 2006. Analysis of intracellular signaling in T cells induced by material captured from APC via Trogocytosis. Autumn Immunology Conference, Chicago, IL.

13. Thauland, T.J.; Koguchi, Y.; Wetzel, S.A.; Varma, R.; Dustin, M.L.; Parker, D.C. 2006. Immunological Synapse Formation By T cell Subsets: TH1 vs. TH2. Keystone Symposia on Lymphocyte Activation and Signaling.

14. Koguchi, Y.; Thauland, T.J.; Wetzel, S.A.; Parker, D.C. 2006. Delivery of Help Through the Immunological Synapse. Keystone Symposia on Lymphocyte Activation and Signaling.

15. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2004) Characterization of Immunological Synapses formed by Anergic T cells. 12th International Congress of Immunology, Montreal, Canada

16. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2003) Peptide-Specific Transfer of MHC:peptide Complexes From Antigen Presenting Cells to T Cells Directly Through the Immunological Synapse . Keystone Symposia: Cell Biology of Antigen Processing and Presentation. Keystone, Colorado

17. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2003) Transfer of MHC:peptide Complexes From Antigen Presenting Cells to T Cells Via the Immunological Synapse . Midwinter Conference of Immunologists Asilomar, CA.

18. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2002) Examining Immunological Synapse Formation By Anergic T cells. Midwinter Conference of Immunologists Asilomar, CA.

19. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2002) Examining Immunological Synapse Formation By Anergic T cells. Keystone Symposia: T Lymphocyte Activation, Differentiation and Death. Keystone, Colorado

20. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2001) Examining the role of Costimulation in the formation of the Immunological Synapse. Gordon Conference on Immunochemistry and Immunobiology.

21. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (2001) The Role of Costimulation in the formation of the Immunological Synapse. 11th International Congress of Immunology, Stockholm, Sweden

22. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (1999) The Effect of MHC Pre-aggregation on T Cell Activation. National Eye Institute Training Grant Meeting.

23. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (1999) Examination of the Kinetic Proofreading Model Using Novel Murine Class II Chimeras. Midwinter Conference of Immunologists. Asilomar, CA

24. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (1998) Examining the Kinetic Proofreading Model Using Novel Murine Class II Chimeras II Capable of Drug Induced Oligomerization. National Eye Institute Training Grant Meeting

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25. Wetzel, S.A.; McKeithan, T.W.; Parker, D.C. (1997) Preparation of Novel Peptide-Loaded Murine MHC Class II Capable of Drug Induced Oligomerization. Western Conference of Immunology, Berkeley, CA.

26. Primus, F.J.; Wetzel, S.A. (1992) Monoclonal Antibody (MAb) Gene Transfer Into Human Colon Carcinoma Cells Elicits Cell Mediated Cytotoxicity. Human Gene Therapy 5:611

27. Cua, D.J.; Wetzel, S.A.; Adler-Moore. J.P.; Sperry, P.J. (1992) Response of Fungal-Infected Epidermal Langerhans Cells to Liposome-Encapsulated and Unencapsulated Amphotericin B. The Journal of Investigative Dermatology 98:598

28. Wetzel, S.A.; Cua, D.J.; Adler-Moore. J.P.; Sperry, P.J. (1991) Uptake of Fungal Cells by Epidermal Langerhans Cells and Subsequent Fungicidal Activity When Incubated with Liposome Encapsulated Amphotericin B. The Journal of Investigative Dermatology 96:626

Mentoring Activities: Graduate Student Major Professor:

• Blair Debuysscher, Ph.D. September 2014 Co-mentored with Dr. Heinz Feldman at the NIAID Rocky Mountain Laboratories. Project: “Characterization Of Nipah Virus Pathogenicity In Vitro And In Vivo And Protection From Disease Using A Single Dose Recombinant Vaccine”

• Tiffany Emmons, M.S. July 2014 Thesis Title: “The Effects of Estrogen in Atrazine-Mediated Foxp3 Induction and Inhibition of CD4+ T Effector T Cells”

• Grant Osborne Ph.D. January 2013 Thesis Title: “Sustained Signaling And Selective Survival Of CD4+ T Cells As A Consequence Of Trogocytosis.”

• Lindsay Thueson, M.S. December 2011 Thesis Title: “Immune Suppression By The Herbicide Atrazine Involves Inhibition Of CD4+ T Cells And Expansion Of Foxp3+ Regulatory T Cells” • Jim Reed, Ph.D. Entered lab in Fall 2014. Expected graduation 2019. His project is examining the role of trogocytosis-mediated signaling on CD4+ T cell cytokine production and subset differentiation.

Graduate Student Thesis Committees

Total of 29 Ph.D. committees and 6 M.S. committees

Completed Committees (year indicates graduation year):

Flavia Pereira Ph.D. – (Biomedical and Pharmaceutical Sciences, 2007)Francisco Leyva Ph.D. – (Toxicology, 2007)David Blake Ph.D. (IMB/Cell and Molecular Biology, 2007)

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Wetzel CV

Jaishree Bankoti Ph.D. – (Toxicology with Immunology Emphasis 2009)Teri Girtsman Ph.D. – (Toxicology with Immunology Emphasis 2009)Marvin Bentley Ph.D. – (IMB/Cell and Molecular Biology, 2010) (Committee Chair)Jenna Benson Ph.D. – (Toxicology with Immunology Emphasis, 2011)Christy Strong Ph.D. – (Biochemistry, 2011)Shawna Graves Ph.D. – (Biochemistry and NIH Graduate Partnership Program, 2011)Tom Simones Ph.D. – (Toxicology with Immunology Emphasis, 2012)Jessica Gilbert M.S. – (IMB/Biochemistry 2009) (Committee Chair)Nandhakumar Thyanidhi Ph.D. – (IMB/Cell and Molecular Biology, 2012 conferred posthumously)Sarah Lacher Ph.D. – (Toxicology Program, 2013)Leah Christensen Ph.D. – (CMMB/Microbiology and Immunology and NIH Graduate Partnership

Program, 2013) (Committee Chair)Nicole Byrnes, M.S. – (BMED/Neuroscience, 2014)Emily Messina Ph.D. – (CMMB/Microbiology and Immunology, 2014) (Committee Chair)Bryan Simmons Ph.D. - (Toxicology, 2015)Moses Leavens M.S. – (Biochemistry and Biophysics, 2015)

Josh Marceau - BMED/GPP Ph.D. , 2016

Juan Palacios-Moreno - BMED/Neuroscience Ph.D. 2017

Joanna Kreitinger – CMMB/Microbiology and Immunology, 2018

Current Sgraduate Student Committee Memberships

Rachel Burmeister - BMED/Toxicology Ph.D. Candidate

Maria Ferrini - BMED/Toxicology Ph.D. Candidate

Miyuki Hayasi – Biochemistry and Biophysics Ph.D. Candidate

Moses Leavens – Biochemistry and Biophysics Ph.D. Candidate

Matthew Sydor - BMED/Toxicology Ph.D. Candidate

Jessica Ray - BMED/Toxicology Ph.D. Candidate

Valery Roman-Cruz - CMMB, Immunology Emphasis, Ph.D. Candidate

Camilla deMattos – CMMB Ph.D Candidate

Shelby Cole - CMMB M.S. Student

Completed Graduate Student Committee Memberships (no degree granted)

Ellen Lark – IMB/IGERT (replaced due to change in research emphasis)

Michalee Moen – IMB/Biochemistry (Withdrew from Program)

Kristina Hurley - BMED/Toxicology (Withdrew from Program)

Jaydene McDaniel – BMED/Neuroscience (Withdrew from Program)

Undergraduate Researchers (19 total)

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Wetzel CV

• Sean Wolf B.S. (2007 – 2009) – Senior Health Analyst, Denver, CO.

• Timmon Hayes B.S. (2008-2010) – Presented her work at the National Conference on Undergraduate Research in 2010. Co-first author on manuscript in preparation. Currently a hospital sanitarian in Helena, MT.

• Lindsay Thueson B.S., M.S. (2009) Entered UM IMB M.S. program in 2009 and completed her thesis in 2011. Currently a 3rd year Physical Therapy Student, University of Montana.

• Sadie Frey B.S. (2009) – currently working as a technician at a startup biotech firm in Seattle, WA.

• Ben Barbee B.S. (2011) - Current status unknown.

• Joanna Kreitlinger B.S. (2011-2012) – Admitted to University of Montana CMMB Ph.D. Program Fall 2013.

• Sarah Sowerwine B.S. (2011) Entered the WWAMI Program of the University of Washington Medical School in 2012.

• Katherine Monsor B.S. (2009 – 2011) Clinical Laboratory Scientist, Great Falls, MT.

• Andrew Lester (2012-2013) – Entered Medical School, University of Arizona, Fall 2014.

• Rana Araholy (2012) – Returned to Saudi Arabia to pursue Medical Technologist Training.

• Rob Lahren (2012) – Optometrist.

• Naomi Crane (2012-14) – Currently a Medical Technologist, Missoula, MT. Presented her work at 2014 American Association of Immunologists meeting, Pittsburgh, PA.

• Kelsy Peprock (2014) – Current status unknown.

• Donna Twedt (2014-15) – Clinical Laboratory Scientist, Missoula, MT.

• Sarah Huntting (2014, 2016) – Left Science.

• Yu Xi (2015-2016) –Currently M.D/Ph.D. Immunology Student at Washington University in St. Louis

• Stephanie Back (2015-2016) – Research Technician for National Forest Service.

• Hannah Fay (2015 - 2017) – Currently a Ph.D. Student in Immunology at New York University

• Austen Grooms (2016 - 2017) – Research Technician at Wright State University, applying for Immunology M.D./Ph.D. admission.

• Coral Strom (2017) – Applying to Dental School

• Mitchell Klomp (2017 – 2018) – Ph.D. Student in virology at University of North Dakota Medical School

• Dawit Mengistu – (2017 – Present) Currently in Lab

• Morgan Stark (2017 – 2019) Completing degree and applying for Medical School

• Madison Reichelt (2018 – Present) Currently in Lab• Connor Bowman (2019 – Present) Currently in Lab

Student Awards

Jim Reed: UM Center for Biochemical Structure and Dynamics summer Fellowship. 2016.

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Douglas Osborne: Student Travel Award for INBRE/COBRE Meeting at Big Sky, MT. Sept 18-20, 2009

Lindsay Thueson: University of Montana Seim Fellowship for The Outstanding Microbiology M.S. Student. 2010.

Timmon Hayes: Grant to cover registration costs of attending National Conference on Undergraduate Research. 2010.

Joanna Kreitinger: Montana Integrative Learning Experiences for Students (MILES) Research Fellowship Fall 2011 and Spring 2012.

Honors and Awards

2016 Recipient, AAI Laboratory Travel Grant Award to attend Immunology 2016.

2011 Recipient, AAI Junior Faculty Travel Award to attend Immunology 2011

2009 Recipient, AAI Junior Faculty Travel Award to attend Immunology 2009

1998 – 2001 Fellow, National Eye Institute Immunology and Molecular Biology Training Grant

1997 Recipient, Tartar Foundation Research Award

Service

University of Montana Division of Biological Sciences / Center for Environmental Health Sciences

2014 Faculty Mentor, Division of Biological Sciences Student Evaluation Committee (committee evaluates faculty performance annually)

2012 – 2013 Member, University of Montana Division of Biological Sciences Graduate Fellowship Awards Committee

2010 – 2014 Organizer and Director, Spring Semester Integrative Microbiology/Cellular and Biochemistry Professional Seminar Series

2007 - Present Chair, Graduate Education Committee, Integrative Microbiology and Biochemistry/ Cellular Molecular and Microbiology Biology Graduate Program, Division of Biological Sciences, The University of Montana, Missoula, MT.

2006 – 2011 Member Division of Biological Sciences Undergratuate Student Awards Committee, Division of Biological Sciences, The University of Montana, Missoula, MT.

2006 – 2011 Member, Division of Biological Sciences Undergraduate Scholarship Committee, The University of Montana, Missoula, MT.

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2006 – 2015 Division of Biological Sciences Website Committee – IMB/CMMBRepresentative

2006 – 2007 Member, Integrative Microbiology and Biochemistry Graduate Program Graduate Evaluations Committee, University of Montana, Missoula, MT.

University of Montana Service

2018 – Present Chair, University of Montana Institutional Animal Care and Use Committee (IACUC)

2016 Chair, Internal Advisory Committee, for Montana Center for Biotechnology Phosphorimager

2015 – Present Member, Internal Advisory Committee, Biospectroscopy Core Research Laboratory

2012 – 2018 Scientific Member, University of Montana Institutional Animal Care and Use Committee (IACUC)

2009 – Present Scientific Director, University of Montana Molecular Histology and Fluorescence Imaging Core Facility, University of Montana, Missoula, MT

2009 – Present Member, University of Montana Center for Environmental Sciences Internal Advisory Committee.

2009 Member, Respiratory Toxicologist Faculty Search Committee, College of Health Professions and Biomedical Sciences, University of Montana, Missoula, MT.

2009-2012 Ad Hoc Member, Toxicology Graduate Program Curriculum Committee Department of Biomedical Sciences, College of Health Professions and Biomedical Scicnes, The University of Montana, Missoula, MT.

2007 Co-chair and Joint Petitioner of an interdepartmental initiative that submitted a proposal to the Montana University System Board of Regents to creating an immunology graduate degree emphasis between DBS and CHPBS that was formally approved in AY07-08.

2006 – Present Director, University of Montana Interdepartmental Immunology Graduate Degree Emphasis. A joint graduate degree track/emphasis in Immunology between The Division of Biological Sciences, the College of Arts and Sciences and the College of Health Professions and Biomedical Sciences.

2006-2011, 2018 Organizer Immunology Work In progress/Journal Club 2018-Present Initiated and directed bi-weekly Immunology work in progress/ journal club

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meetings drawing researchers and students from across UM campus interested in Immunology research and education.

2006 – Present UM Immunology Mail List Established and maintain the UM Immunology mail list

2006 - 2008 Member, University of Montana Interdepartmental Fluorescence Instrumentation Working Group

Community Sevice

2014 - Present Chair of Microbiology Section Judging and Grand Awards Judge Montana State Science Fair

2014 - Present Science Outreach Presenter – Fluorescence Microscopy in Biology. Missoula, Montana Big Sky High School AP Biology Class

2012 Guest Lecture,Osher Life Long Learning Institute at UM (MOLLI) “Vaccine Development”

2009 Participant Habitat for Humanity Missoula Science Day

2009 – Present Guest Lecture, Hubel Elementary School 3rd and 4th grade, Des Moines, IA. “Scientists as Expolorers” and a research talk on the health effects of Atrazine exposure in drinking water.

2002-2005 Participant, School Science Outreach Program (Program to assist Oregon Teachers with Science Education), Oregon Health & Science University, Portland, OR

Professional Service

2016 External Reviewer, North Carolina Biotechnology Center Grant program

2016 External Reviewer, University of Nebraska-Lincoln “Biomedical Research Seed Grants” for the UN-L Office of Research and Economic Development

2014 Member, NIH Study Section: ZES1-JAB-J (AU): The Role of Environmental Exposures in the Development of Autoimmune Disease.

2014 Preliminary Grant Reviewer/Consultant for Junior Faculty at Mississippi State University College of Veterinary Medicine

2012 Reviewer of text book Writing Science in Plain English by Anne Greene (University of Chigaco Press)

2011 Reviewer of Immunology Textbook Kuby Immunology: Chapter 4: Receptors and Signaling: Cytokines and Chemokines (W.H. Freeman and Company Publishers)

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2010 Session Moderator (Biology and Psychology Sessions) National Conference on Undergraduate Research, Missoula, MT.

2005 - 2009 Ad hoc Grant Reviewer for Arthritis Research Council, U.K.

2005 – Present Ad hoc reviewer for the following Journals:Immunity ScienceThe Journal of Immunology Cytometry Part AEuropean Journal of Immunology Journal of Biological ChemistryJournal of Immunological Methods PLOS OneNature Protocols Nature MethodsAnalytical Biochemistry Histochemistry and Cell BiologyJournal of Clinical Investigation DifferentiationToxicological Sciences HistochemieImmunological InvestigationsClinical and Experimental Pharmacology and PhysiologyProceedings of the National Academy of Science, USA

Professional Association Memberships2005 – Present Member - American Association of Immunologists2009 – 2014 Member - American Association of Blood Banking

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Grant History

Active Grants:

• R03AI122167 2/28/16 – 1/31/18 NIH/NIAID $50,000/year direct costs

Determining the role of trogocytosis-mediated signaling on CD4 T cell phenotype and effector functionsRole: PI

Completed Grants

• 1531520 (Ross) 08/01/15 – 7/31/18 National Science Foundation$323,285 direct costs

MRI: Acquisition of a Time-Resolved Confocal Microscope for Research and Education, Role: Co-PI

• S10 OD021806 (Ross) 2/01/16 – 1/31/17 NIH $554,886 direct costs

• Shared Instrument Grant (S10) Acquisition of a Nikon A1 confocal laser-scanning microscope with PicoQuant FCS & FLIM upgradeRole: Co-PI

Murdock Charitable Trust Grant – (Ross) May 2016 $213,800Cost Match for NSF Time-resolved Confocal Microscope Role: Co-PI

• P30 RR033379-A101 University of Montana CEHS COBRE III NIH/NIGMSMolecular Histology and Fluorescence Imaging Core. This is one of three core components of the UM CEHS COBRE III application. MHFI grant component written by Scott Wetzel. Role: MHFI Scientific Director

• R03ES022463-01 12/1/12 – 11/30/14 NIH/NIEHS $50,000/year direct costs

Determining the Role of Atrazine and Atrazine-Induced Estrogen in Increasing CD4+ Regulatory T CellsRole: PI

• MRA 801University of Montana University Grant Program 2012 $2,631Examining sustained singaling after trogocytosis.Role: PI

• MRA 404 University of Montana University Grant Program 2011 $4,570Immune Modulation by Sustained Signaling after TrogocytosisRole: PI

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Wetzel CV

• MRA 380 University of Montana University Grant Program 2010 $2,500Microscopic analysis of immunological synapses formed between CD4+ T cells as a result of trogocytosis. Role: PI

• RR024535 (Ross) 2009 NIH/NCRR $437,000

Shared Instrumentation Grant (S10) to purchase Olympus FV1000 Laser Scanning Confocal Workstation for University of Montana Molecular histology and Fluorescence Imaging Core Facility.Role: Co-PI

• P20RR017670-06 (Holian) 07/01/2007 – 05/31/2010 NIH/NCRR $105,000/year

We will examine the kinetics of T cell – APC interactions leading to mature Immunological Synapse (IS) formation by live-cell, 3D microscopy after exposure of T cells and/or APC to the Herbicide Atrazine. We will characterize the molecular constituents along with their spatial and temporal segregation in these ISs. We will also examine the potential biasing of the primary T cells activated in vitro in the presence or absence Atrazine to TH1, TH2, TH17, or TReg effector phenotypes.Role: Subproject PI

• RO1 ES015294 (Holian) 12/01/2006 – 11/30/2011 NIH/NIEHS 10% Effort $8400/year

Defining the roles of macrophage subsets and NK lymphocytes in silicosisRole: Key Collaborator

• P20 RR17670 (Holian) 07/01/2006 – 06/30/2007CEHS Faculty Development $150,000The overall goals of this COBRE are to develop a highly interactive critical mass of investigators and support cores that will lead to an increase in the number of R01-funded investigators, multi-investigator grants and improved research environment for undergraduates, graduate students and postdoctoral fellows.Role: New Faculty hire

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