12
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004) HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science Enterprise (ESE) C.C. Trees and J.L. Mueller Center for Hydro-Optics and Remote Sensing (CHORS) San Diego State University ([email protected])

HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

  • Upload
    others

  • View
    5

  • Download
    0

Embed Size (px)

Citation preview

Page 1: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science

Enterprise (ESE)

C.C. Trees and J.L. Mueller

Center for Hydro-Optics and Remote Sensing (CHORS)San Diego State University([email protected])

Page 2: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

MODIS Data Products Program - 4,825 samples/yr

Method Comparison

Co-elution of mono- and divinyl Chl a

1. Wright et al. (1991) C18 Method (SIMBIOS)

Channels ratio (436 & 450nm)

Physical separation of mono- and divinyl chl a

1. Goericke and Repeta (1993) C8 Method

2. **Van Heukelem and Thomas (2001) C8 with

column heater Method

HPLC Phytoplankton Pigment Protocols(Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V)

Page 3: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Minutes

2 4 6 8 10 12 14 16 18 20 22 24 26

mAU

0

25

50

75

100

125

150

mAU

0

25

50

75

100

125

150

PDA-284nm20040702007

Vitamin E Internal Standard (284 nm)

Vitamin E Internal Standard (284 nm)

Zeax

anth

in

Div

inyl

Chl

b

Div

inyl

Chl

aM

onov

inyl

Chl

a

Vita

min

E

β,β

Car

oten

e

Internal Standard

Page 4: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Minutes

2 4 6 8 10 12 14 16 18 20 22 24 26

mA

U

-10

0

10

20

30

mA

U

-10

0

10

20

30

PDA-664nm20040702007

Divinyl & Monovinyl Chl a(664 nm)

Divinyl & Monovinyl Chl a(664 nm)

Div

inyl

Chl

b

Mon

ovin

yl C

hl a

Div

inyl

Chl

a

Min ut es20 .6 20. 8 21. 0 2 1. 2 21. 4 21 .6 2 1. 8 22. 0 22 . 2 22. 4 22 .6 2 2. 8 23. 0 23. 2 2 3. 4

mA

U

0

10

20

30

mA

U

0

10

20

30

P D A-664n m2 0040 7020 07

Divinyl and Monovinyl Chl a Separation

Page 5: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Minutes

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

mA

U

0

10

20

30

40

50

60

mA

U

0

10

20

30

40

50

60PDA-450nm20040701002

Culture Mixture (450 nm)

Culture Mixture (450 nm)

Chl

c3

Inje

ctio

n S

pike

Per

idin

in

Allo

xant

hin

Fuco

xant

hin

β.ε

caro

tene

Mon

ovin

yl C

hl a

Dia

toxa

nthi

n

19’-H

exan

oylo

xyfu

coxa

nthi

n

Zeax

anth

in

Mon

ovin

yl C

hl b

Mon

ovin

yl C

hls

a’, a

Chl

c2

Dia

dino

xant

hin

Pra

sino

xant

hin

β.β

caro

tene

Mixture of Pigments from Cultures

Page 6: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

HPLC Samples HPLC Samples HPLC SamplesInvestigators Agency/Program Cruise Name FY04-05 FY05-06 FY06-07

Campbell NASA MODIS Intercal/Dowell's Grant 50 50 50Carder NASA MODIS HAB's 50 50 50Clark NASA MODIS MOBY 400 0 0Cota NASA MODIS Polar Work 300 300 300

Dierssen NASA MODIS Ackleson/ Rhode Island 50 50 50Gould NASA MODIS Coastal Optical Cruises 200 200 200Hooker NASA MODIS SeaHARRE-3 (1st yr) & Cr. 90 125 125Letelier NASA MODIS GLOBEC (previous cruises) 200 0 0Marra NASA MODIS Ship of Opportunity 50 50 50

Muller-Karger NASA MODIS Cariaco Basin 230 230 230Nelson NASA MODIS BBOP 50 50 50Siegel NASA MODIS Plumes & Blooms 450 450 450

Stramski NASA MODIS Carbon/High Latitude 200 200 50Stumpf NASA MODIS Estuarine and Coastal 100 100 100

MODIS EOS Total 2,420 1,855 1,705Chavez NASA (3 grants) 250 250 250

Lorenzen NASA Ecohab Gulf of Mexico 0 0 0Mannino NASA IDS 120 120 120Mannino NASA NIP 0 135 135Mitchell NASA Ecohab Gulf of Mexico/California 200 200 400Moline NASA NIP 100 200 200

Siegel and Nelson NASA Core Repeat Survey CDOM 250 250 0Sosik NASA IDS North Atlantic Bight 100 100 100

Subramaniam NASA Core 125 0 0Other NASA Total 1,145 1,255 1,205

NASA TOTAL 3,565 3,110 2,910

Investigators Agency/Program Cruise Name FY04-05 FY05-06 FY06-07Mitchell NOAA AMLR 204 0 0Mitchell NSF Blue Water Zone Cr 256 0 0Moline NSF Monterey Bay 200 300 300

Siegel and McGillicuddy NSF EDDIES 600 1,200 0TOAL REQ UESTED 4,825 4,610 3,210

HPLC Samples Requested

Page 7: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Filtration

1. GF/F glass fiber filters (25 mm)

2. Positive pressure filtration recommended

3. Filtration Volumes (green as possible)

a. Oligotrophic waters - 3-4 liters

b. Mesotrophic waters - 1-2 liters

c. Eutrophic waters - 0.5-1 liter

Sample Handling, Storage and Shipping

1. Fold filters in half with filtered sides facing in.

HPLC Phytoplankton Pigment Protocols

Page 8: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Sample Handling, Storage and Shipping (cont)

2. Place in aluminum foil envelops, cryogenic vials or

HistoPrep tissue capsules.

3. Label samples with a sequential number.

4. Store and ship (dry shipper) in liquid nitrogen (LN2).

5. PI’s pay for all shipping costs (includes costs

for returning LN2 shipping container to PI).

Recordkeeping (electronically transmitted to CHORS)

1. For each sample the volume filtered, station number,

date, time, depth, lat and long will be sent.

HPLC Phytoplankton Pigment Protocols

Page 9: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Positive Pressure Filtration System

Page 10: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Bottle Cap Valves/Hardware

Page 11: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Nalgene LDPE Carboys with Tubulation, Fisher (2L #02-962B & 4L #02-962C)

Nalgene Polypropylene Stopcocks with PTFE Plug, Fisher (2mm #14-630-1A)

Tee Barbed Fitting, Cole Palmer (1/4" #U06462-20)

Elbow Barbed Fitting, Cole Palmer (1/4" #U06461-10)

In-Line Delrin Filter Holder, VWR (25mm, Pall Gelman #28144-255)

PVC Labcock Valve, RyanHerco (1/4" MT X H #5309.102)

PVC Bushing N870, RyanHerco (1/2x1/4" MTXT Spear #3839S072)

Gast Oil less Diaphragm-Type Pressure/Vacuum Pump, Fisher (#01-092-29)

Positive Pressure Filtration System

Page 12: HPLC Phytoplankton Pigment Measurements: Continuation of … · 2016. 7. 29. · HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V) CHORS,

CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)

Liquid Nitrogen Refrigerator (Dry Shipper)

Taylor-Wharton Cryogenics

Model Number 3DSLN2 Capacity - 3 liters*Static Hold Time - 20 days*Working Time - 14 days

Hard-shell Shipping Case

*Nominal values

Midwest Scientific (www.midsci.com)Thermolyne Arctic Express (Fisher)Taylor-Wharton CX Series (Cole-

Parmer)Taylor-Wharton LD Model CP30 (VWR)