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CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science
Enterprise (ESE)
C.C. Trees and J.L. Mueller
Center for Hydro-Optics and Remote Sensing (CHORS)San Diego State University([email protected])
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
MODIS Data Products Program - 4,825 samples/yr
Method Comparison
Co-elution of mono- and divinyl Chl a
1. Wright et al. (1991) C18 Method (SIMBIOS)
Channels ratio (436 & 450nm)
Physical separation of mono- and divinyl chl a
1. Goericke and Repeta (1993) C8 Method
2. **Van Heukelem and Thomas (2001) C8 with
column heater Method
HPLC Phytoplankton Pigment Protocols(Bidigare et al., 2003; NASA/TM-2003-211621/Rev 4 - Vol. V)
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Minutes
2 4 6 8 10 12 14 16 18 20 22 24 26
mAU
0
25
50
75
100
125
150
mAU
0
25
50
75
100
125
150
PDA-284nm20040702007
Vitamin E Internal Standard (284 nm)
Vitamin E Internal Standard (284 nm)
Zeax
anth
in
Div
inyl
Chl
b
Div
inyl
Chl
aM
onov
inyl
Chl
a
Vita
min
E
β,β
Car
oten
e
Internal Standard
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Minutes
2 4 6 8 10 12 14 16 18 20 22 24 26
mA
U
-10
0
10
20
30
mA
U
-10
0
10
20
30
PDA-664nm20040702007
Divinyl & Monovinyl Chl a(664 nm)
Divinyl & Monovinyl Chl a(664 nm)
Div
inyl
Chl
b
Mon
ovin
yl C
hl a
Div
inyl
Chl
a
Min ut es20 .6 20. 8 21. 0 2 1. 2 21. 4 21 .6 2 1. 8 22. 0 22 . 2 22. 4 22 .6 2 2. 8 23. 0 23. 2 2 3. 4
mA
U
0
10
20
30
mA
U
0
10
20
30
P D A-664n m2 0040 7020 07
Divinyl and Monovinyl Chl a Separation
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Minutes
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28
mA
U
0
10
20
30
40
50
60
mA
U
0
10
20
30
40
50
60PDA-450nm20040701002
Culture Mixture (450 nm)
Culture Mixture (450 nm)
Chl
c3
Inje
ctio
n S
pike
Per
idin
in
Allo
xant
hin
Fuco
xant
hin
β.ε
caro
tene
Mon
ovin
yl C
hl a
Dia
toxa
nthi
n
19’-H
exan
oylo
xyfu
coxa
nthi
n
Zeax
anth
in
Mon
ovin
yl C
hl b
Mon
ovin
yl C
hls
a’, a
”
Chl
c2
Dia
dino
xant
hin
Pra
sino
xant
hin
β.β
caro
tene
Mixture of Pigments from Cultures
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
HPLC Samples HPLC Samples HPLC SamplesInvestigators Agency/Program Cruise Name FY04-05 FY05-06 FY06-07
Campbell NASA MODIS Intercal/Dowell's Grant 50 50 50Carder NASA MODIS HAB's 50 50 50Clark NASA MODIS MOBY 400 0 0Cota NASA MODIS Polar Work 300 300 300
Dierssen NASA MODIS Ackleson/ Rhode Island 50 50 50Gould NASA MODIS Coastal Optical Cruises 200 200 200Hooker NASA MODIS SeaHARRE-3 (1st yr) & Cr. 90 125 125Letelier NASA MODIS GLOBEC (previous cruises) 200 0 0Marra NASA MODIS Ship of Opportunity 50 50 50
Muller-Karger NASA MODIS Cariaco Basin 230 230 230Nelson NASA MODIS BBOP 50 50 50Siegel NASA MODIS Plumes & Blooms 450 450 450
Stramski NASA MODIS Carbon/High Latitude 200 200 50Stumpf NASA MODIS Estuarine and Coastal 100 100 100
MODIS EOS Total 2,420 1,855 1,705Chavez NASA (3 grants) 250 250 250
Lorenzen NASA Ecohab Gulf of Mexico 0 0 0Mannino NASA IDS 120 120 120Mannino NASA NIP 0 135 135Mitchell NASA Ecohab Gulf of Mexico/California 200 200 400Moline NASA NIP 100 200 200
Siegel and Nelson NASA Core Repeat Survey CDOM 250 250 0Sosik NASA IDS North Atlantic Bight 100 100 100
Subramaniam NASA Core 125 0 0Other NASA Total 1,145 1,255 1,205
NASA TOTAL 3,565 3,110 2,910
Investigators Agency/Program Cruise Name FY04-05 FY05-06 FY06-07Mitchell NOAA AMLR 204 0 0Mitchell NSF Blue Water Zone Cr 256 0 0Moline NSF Monterey Bay 200 300 300
Siegel and McGillicuddy NSF EDDIES 600 1,200 0TOAL REQ UESTED 4,825 4,610 3,210
HPLC Samples Requested
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Filtration
1. GF/F glass fiber filters (25 mm)
2. Positive pressure filtration recommended
3. Filtration Volumes (green as possible)
a. Oligotrophic waters - 3-4 liters
b. Mesotrophic waters - 1-2 liters
c. Eutrophic waters - 0.5-1 liter
Sample Handling, Storage and Shipping
1. Fold filters in half with filtered sides facing in.
HPLC Phytoplankton Pigment Protocols
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Sample Handling, Storage and Shipping (cont)
2. Place in aluminum foil envelops, cryogenic vials or
HistoPrep tissue capsules.
3. Label samples with a sequential number.
4. Store and ship (dry shipper) in liquid nitrogen (LN2).
5. PI’s pay for all shipping costs (includes costs
for returning LN2 shipping container to PI).
Recordkeeping (electronically transmitted to CHORS)
1. For each sample the volume filtered, station number,
date, time, depth, lat and long will be sent.
HPLC Phytoplankton Pigment Protocols
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Positive Pressure Filtration System
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Bottle Cap Valves/Hardware
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Nalgene LDPE Carboys with Tubulation, Fisher (2L #02-962B & 4L #02-962C)
Nalgene Polypropylene Stopcocks with PTFE Plug, Fisher (2mm #14-630-1A)
Tee Barbed Fitting, Cole Palmer (1/4" #U06462-20)
Elbow Barbed Fitting, Cole Palmer (1/4" #U06461-10)
In-Line Delrin Filter Holder, VWR (25mm, Pall Gelman #28144-255)
PVC Labcock Valve, RyanHerco (1/4" MT X H #5309.102)
PVC Bushing N870, RyanHerco (1/2x1/4" MTXT Spear #3839S072)
Gast Oil less Diaphragm-Type Pressure/Vacuum Pump, Fisher (#01-092-29)
Positive Pressure Filtration System
CHORS, SDSU MODIS Team Meeting (Washington, D.C., 13-15 Jul 2004)
Liquid Nitrogen Refrigerator (Dry Shipper)
Taylor-Wharton Cryogenics
Model Number 3DSLN2 Capacity - 3 liters*Static Hold Time - 20 days*Working Time - 14 days
Hard-shell Shipping Case
*Nominal values
Midwest Scientific (www.midsci.com)Thermolyne Arctic Express (Fisher)Taylor-Wharton CX Series (Cole-
Parmer)Taylor-Wharton LD Model CP30 (VWR)