Copyright © 2004 John Wiley & Sons, Ltd. BIOMEDICAL CHROMATOGRAPHY Biomed. Chromatogr. 18: 475 (2004) Published online in Wiley InterScience (www.interscience.wiley.com). Book review HPLC of Peptides and Proteins: Methods and Protocols, edited by MARIE-ISABEL AGUILAR (Methods in Molecular Biology, Volume 251, Series Editor J. M. Walker). Humana Press, Totowa, NJ, 2003, US$99.50, 413 pp, ISBN: 0-86903-977-3. HPLC of Peptides and Proteins is a detailed collection of practical information covering basic theory, com- monly used advanced techniques in protein characterizations together with a series of particularly interesting case studies. The first few chapters indicate the basic principles and are very helpful for newcomers in this field. This is followed by more advanced analytical proteome applications with detailed methodologies for the separation and analyses of peptides, proteins, post-translationally modified proteins, glycopeptides, glycoproteins and membrane proteins. Methods range from simple capillary to large-scale preparative isola- tion. Overall, this book is well written and the early chapters are particularly suitable for beginners in HPLC. The preparation techniques would be very useful indeed. Advice on potential problems and pitfalls with each technique is well documented. In summary, HPLC of Peptides and Proteins is a very useful labor- atory companion or reference guide for researchers in pursuit of peptide and protein characterizations. Naveed Ahmed Khan School of Biological and Chemical Sciences, Birkbeck College University of London, London WC1E 7HX, UK [DOI: 10.1002 /bmc.422] BOOK REVIEW

HPLC of peptides and proteins: methods and protocols, edited by Marie-Isabel Aguilar (methods in molecular biology, volume 251, series editor J. M. Walker). Humana Press, Totowa, NJ,

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Page 1: HPLC of peptides and proteins: methods and protocols, edited by Marie-Isabel Aguilar (methods in molecular biology, volume 251, series editor J. M. Walker). Humana Press, Totowa, NJ,

Chromatographic determination of herbicide residues 475BOOK REVIEW

BIOMEDICAL CHROMATOGRAPHYBiomed. Chromatogr. 18: 475 (2004)Published online in Wiley InterScience (www.interscience.wiley.com).

Book review

HPLC of Peptides and Proteins: Methods andProtocols, edited by MARIE-ISABEL AGUILAR(Methods in Molecular Biology, Volume 251, SeriesEditor J. M. Walker). Humana Press, Totowa, NJ,2003, US$99.50, 413 pp, ISBN: 0-86903-977-3.

HPLC of Peptides and Proteins is a detailed collectionof practical information covering basic theory, com-monly used advanced techniques in protein character-izations together with a series of particularly interestingcase studies. The first few chapters indicate the basicprinciples and are very helpful for newcomers in thisfield. This is followed by more advanced analyticalproteome applications with detailed methodologiesfor the separation and analyses of peptides, proteins,post-translationally modified proteins, glycopeptides,

glycoproteins and membrane proteins. Methods rangefrom simple capillary to large-scale preparative isola-tion. Overall, this book is well written and the earlychapters are particularly suitable for beginners inHPLC. The preparation techniques would be very use-ful indeed. Advice on potential problems and pitfallswith each technique is well documented. In summary,HPLC of Peptides and Proteins is a very useful labor-atory companion or reference guide for researchers inpursuit of peptide and protein characterizations.

Naveed Ahmed KhanSchool of Biological and Chemical Sciences,

Birkbeck College University of London,London WC1E 7HX, UK

[DOI: 10.1002/bmc.422]

BOOK REVIEW