HPLC Basic Course 2.Principle and System Configuration of HPLC(2):Hitachi High-Technologies Corporation

http://www.hitachi-hitec.com/global/science/lc/lc_basic_2.html[1/17/2015 10:53:18 PM]

News & Events Sitemap

| Home | About Us | Investor Relations | Global Network | Products & Services | Customer Service |

Science & Medical Systems > Products > HPLC/Amino Acid Analyzer

HPLC Basic Course2. Principle and System Configuration of HPLC(2)

HPLC Basic Course

Philosophy of Qualitative analysis and Quantitative analysis by chromatography

Qualitative analysis A standard sample of aspartame was measured, yielding a peak at 12.5 minutes. This

peak corresponds to aspartame, itself.Next, a sample prepared from a beverage was measured under the same conditions, yielding a number of peaks. The peak appearing at 12.5 minutes can be regarded as that of aspartame (Figure 1).The following conditions were the same: the type of fillers, column sizes, column temperature, composition of the mobile phase, and flow rate.

Figure 1. Example of aspartame measurement

Quantitative analysis The height and area of a peak are proportional to the concentration of the corresponding

component. A calibration curve is created using the standard sample. The concentration of aspartame in the beverage can be determined from the peak area of the detected aspartame.

Search by Google

> Advanced Search

Science & Medical Systems

Topics

Global Network

Products

UV-Visible/NIR Spectrophotometer

Fluorescence Spectrophotometer

Atomic Absorption Spectrophotometer

ICP-OES/ICP-MS

HPLC/Amino Acid Analyzer

ChromasterUltra Rs

Chromaster

Chromaster Driver for Waters Empower2 Software

Chromaster system Manager

Primaide

L-8900

HPLC Columns LaChromUltra II C18

HPLC Columns LaChrom Series

Clinical Analyzer

XRF Analysis

XRF Coating Thickness Gauge

Thermal Analysis

Potentiometric Titrator

Karl Fischer Titrator

Mercury Analyzer

Applications

Contact Us

Sitemap

HPLC Basic Course 2.Principle and System Configuration of HPLC(2):Hitachi High-Technologies Corporation

http://www.hitachi-hitec.com/global/science/lc/lc_basic_2.html[1/17/2015 10:53:18 PM]

pagetop

Attention should be given to the fact that a qualitative analysis includes many uncertainties. Other components may have been eluted together with aspartame.LC and GC systems are good at determining the content of a certain component in a sample, rather than the types of the components of a sample. Performance of a quantitative analysis requires the preparation of a calibration curve. It is very difficult to perform the qualitative or quantitative analysis of a component for which a standard sample is not available.

The next chapter is "Configuration of an HPLC system".

Ultra High Performance Liquid Chromatograph ChromasterUltra Rs

High Performance Liquid Chromatograph Chromaster

High Performance Liquid Chromatograph Primaide

HPLC Columns LaChrom Series

hitachi-hitec.comHPLC Basic Course 2.Principle and System Configuration of HPLC(2):Hitachi High-Technologies Corporation

5jZS9sYy9sY19iYXNpY18yLmh0bWwA: Q: input: