How to Display and Download ENCODE Data

Mike Pazin

NHGRI, NIH

Goals of ENCODE

• Catalog all functional elements in the genome

• Freely available resource for all biologists

• Human as well as other species

• Project components:

– Data generation

– Data analysis

– Data repository

How Can ENCODE Data Be Displayed?

http://encodeproject.org Handout

How Can The Display Be Configured?

http://encodeproject.org

ENCODE Browser, Locus of Interest

http://encodeproject.org

ENCODE Data From HaploReg

1

2 3

http://www.broadinstitute.org/mammals/haploreg/haploreg.php Ward and Kellis, Nucleic Acids Research 40-D930, 2011

Handout

ENCODE Data From RegulomeDB

1

2

3

http://regulome.stanford.edu/ ; Boyle…Snyder, Genome Research 22-1790,2012

Handout

RegulomeDB Disease Database

http://regulome.stanford.edu/GWAS; Schaub…Snyder, Genome Research 22-1748,2012

How Can The Display Be Configured?

Handout

ENCODE Experiment Matrix

http://encodeproject.org

How Can ENCODE Data Be Downloaded?

http://encodeproject.org

Publications

http://encodeproject.org

Over 170 Publications Using ENCODE Data

Frazer lab, Nature 470-264,2011 Anderson lab, Nature Genetics 44-1137,2012

ENCODE Software Tools

http://encodeproject.org

ENCODE Citation

ENCODE Consortium Brad Bernstein (Eric Lander, Manolis Kellis/Luke Ward, Tony Kouzarides)

Ewan Birney (Jim Kent, Mark Gerstein, Bill Noble, Peter Bickel, Ross Hardison, Zhiping Weng)

Greg Crawford (Ewan Birney, Jason Lieb, Terry Furey, Vishy Iyer)

Jim Kent (David Haussler, Kate Rosenbloom)

John Stamatoyannopoulos (Evan Eichler, George Stamatoyannopoulos, Job Dekker, Maynard Olson, Michael Dorschner, Patrick Navas, Phil Green)

Mike Snyder (Kevin Struhl, Mark Gerstein, Peggy Farnham, Sherman Weissman)

Rick Myers (Barbara Wold)

Scott Tenenbaum (Luiz Penalva)

Tim Hubbard (Alexandre Reymond, Alfonso Valencia, David Haussler, Ewan Birney, Jim Kent, Manolis Kellis, Mark Gerstein, Michael Brent, Roderic Guigo)

Tom Gingeras (Alexandre Reymond, David Spector, Greg Hannon, Michael Brent, Roderic Guigo, Stylianos Antonarakis, Yijun Ruan, Yoshihide Hayashizaki)

Zhiping Weng (Nathan Trinklein, Rick Myers)

NHGRI: Elise Feingold, Peter Good, Laura Dillon, Rebecca Lowdon, Leslie Adams, Caroline Kelly, Shaila Chhibba, Sherry Zhou, Katya Vaydylevich

Additional ENCODE Participants: Elliott Marguiles, Eric Green, Job Dekker, Laura Elnitski, Len Pennachio, Jochen Wittbrodt

.. and many senior scientists, postdocs, students, technicians, computer scientists, statisticians and administrators in these groups

Questions? NHGRI booth (# 825) noon- 1 PM; pazinm@mail.nih.gov

What Do The Data Mean?

• Some standard interpretations-

– RNA

– Histone modifications

– DNase

– Transcription Factor ChIP

How Can ENCODE Data Be Used?

• A standard problem: many genetic findings for human disease map to non-protein coding regions of the human genome – What is the functional variant? – What is the target gene? – What is the target cell type? – What is the function of the variant?

• Standard Browser view for loci of interest • HaploReg and RegulomeDB searches for loci of interest • Search a cell type across all data types for loci of interest • Search a data type across all cell types for loci of interest

ENCODE Browser, Locus of Interest

http://encodeproject.org

ENCODE Browser, Cells of Interest

http://encodeproject.org

NIH Roadmap Epigenomic Mapping Consortium

Range of cells/tissues covered:

Currently 125 cell/tissue types represented including….

iPS and ES cells, some differentiated forms

Fetal tissues (heart, brain, kidney, lung, others)

Adult primary cells and tissues (hematopoietic, brain regions,

breast cell types, liver, kidney, colon, muscle, adipocytes, others)

Some samples will have:

Expanded panel of histone modifications (currently 20

additional)

A public community resource of epigenomic data in primary human cells and tissues.

Most samples will have:

DNA methylation data (RRBS, MRE-seq, MeDIP-seq, whole

genome bisulfite seq)

ChIP-seq data (currently H3K27me3, H3K36me3, H3K4me1,

H3K4me3, H3K9me3)

DNase I hypersensitivity data

Gene expression data (arrays or RNA-seq)

Can download:

.wig, .bed, some .bam, some SRA, working on peak calls

http://roadmapepigenomics.org – Find and view data,

protocols, links to other sites associated with the program.

View Roadmap data at http://genome.ucsc.edu via TRACK HUB

– click ‘track hubs’ to load Roadmap data and summary data

tracks on UCSC. Where is the data?

Find data, protocols, and analysis/viewing tools from the

Roadmap Epigenomic Mapping Consortium at these sites:

http://roadmapepigenomics.org

http://ncbi.nlm.nih.gov/epigenomics

http://ncbi.nlm.nih.gov/geo/roadmap/epigenomics

http://epigenomeatlas.org

http://vizhub.wustl.edu

http://genome.ucsc.edu (via track hub)

• More than 30 papers in Nature, Genome Research, Genome Biology, Science, Cell

• Publishing innovations • ENCODE increased our

understanding of non-coding DNA, and human disease

Recent ENCODE Publications

From www.nature.com/encode

ENCODE Structure

Gene Models

RNA TF

Binding

Data Coordination Center

Data Analysis Center

Element ID

Chromatin States

Histone Mods DNase DNAme

Modified from PLoS Biol 9-e1001046,2011

What Data Are Available?

Modified from PLoS Biol 9-e1001046,2011

ENCODE Dimensions

From Ewan Birney

Raw Genomic Coverage of Elements

Biochemical Mark Genomic Coverage

Any ENCODE mark 80 %

Any ENCODE RNA 62 %

Any Histone modification 56 %

DHS or TF ChIP 19.4 %

Open chromatin 15.2 %

TF ChIP 8.1 %

DHS footprint 5.7 %

Purifying selection ~3-8 %

Exons (GENCODE) 2.94 %

Protein-coding regions (GENCODE) 1.22 %

Nature 489-57,2012