How much money we need for a research project ?

• Financial support is a must for undertaking any research work.

• Unjustified request for fund may lead to rejection of the project

• Too less of finance may lead to difficulty in completion of a proposed project.

• Budget is a rational approach towards requirement of fund for research.

What is budget?

• A budget ensures that sufficient resources (financial, personnel, and infrastructure /equipment) are available to complete the research work that is being proposed.

• It should be based on a full analysis of research project costs, and aim to get the sponsor to pay for as many of those costs as possible, while ensuring that both the sponsoring agency and Institutional guidelines are followed.

Justification

• The budget justification narrative, required by most agencies, is an important opportunity to describe exactly how budget items will be used to complete the study.

• A carefully constructed detailed budget increases the likelihood of securing funding because it documents the research team’s expertise to the funding agency. An accurate budget is essential to provide the necessary resources to conduct the study.

At what stage it should be done?• Preparing a research

proposal budget can seem like an overwhelming responsibility, especially for new investigators.

• Preparing a detailed budget during the proposal stage can minimize budget management difficulties once the project is funded.

Budget of a research project

Nonrecurring Expenditure

Refer to one time expenditure

Equipment for Sample processing

Equipment for Storage

Instruments for Quantification

Instruments for Quantification

• Spectro flurometer (8-10L): Measurement of fluroescence.

• Nephlometer (2-3L) : Immuno-turbidimetry• Chemiluminiscence (4-5L) :• Multimode reader (12-15L)

Instruments for Quantification

Costly and big instruments• Gas Chromatography (15-20L) • HPLC (30-50L) • Atomic absorption spectrophotometer (30-

40L)• Flowcytometer (50-60L)• Flowcytometer with cell sorter ( 75- 100L)

Instruments for Quantification

Equipments for Molecular Biology research

• Thermo cycler personal (2-3L) gradient ( 5-6L)• Gel Electrophoresis (2-3 L)• Gel documentation System (5-6L)• Real time PCR (20-25 L)• Gene analysis system (40L)

BUDGET FOR EQUIPMENT*

S.No. Generic name of equipment with make and model

Imported / Indigenous

Estimated cost (in foreign currency also)

Spare time for other users (in %)

1.Real Time PCR (2 plex with HRM) Imported Rs 16.3 lakhs

(US$32,000) 50%

2.Inverted microscope with

digital camera and computer

Imported Rs 5 lakhs (US$10,100) 50%

3. Deep freezer (minus 20º C) Indigenous Rs 75,000 20%

4. Liquid nitrogen containers (35 L x 2) Indigenous Rs 80,000 20%

Justification for the proposed equipment• The Department of Biochemistry, University College of Medical

Sciences, Delhi, where the proposed research project work will be executed, has all the necessary equipments as mentioned in “facilities/equipments being extended by parent institution” except the above mentioned equipment.

• A Real time PCR machine (Q-PCR), critical for quantifying gene expression patterns, is essential for achieving the objectives.

• The department is initiating cell / tissue culture facility and have all the other equipments such as Laminer flow, CO2 incubator except an inverted microscope with a digital camera attached to a computer, for detailed image analysis.

• We will also require liquid nitrogen containers that will be used for storing the cell lines.

• A dedicated deep freezer will be essential for storing tissue culture material, temperature sensitive reagents and samples for the project as the existing freezers are overloaded.

Recurring Expenditure

Repeating Expenditure Manpower Consumable Travel Contingency Overhead • Expenditure to be mentioned Year wise separately for all the

above mentioned items depending on the experimental protocol.

ManpowerResearch PersonnelHelp in planning, execution & data collection • Research associate ( 23-24 K*) • Medical officer ( 20K*)• Senior research fellow ( 18-20 K* )• Junior Research Fellow ( 16-18 K* )• Research Assistant ( 21K *)

* + HRA @ as admissible

Technical Personnel

• Technical Officer/ Computer programmer (29.5K*)

• Data entry operator/ Statistician (26.5 K*) • Technician/ Data entry operator/ Nurse / Field

worker ( 17.5*)• Lab. Assistant/ data entry operator Jr ( 15.5K*) • Office assistant/ Driver (11.9 K*)• Lab. Attendant/ Animal attendant ( 11K*) * Consolidated salary

BUDGET FOR SALARIES / WAGESBUDGET (In rupees)

1st year (m.m*)

2nd year (m.m*)

3rd year (m.m*)

Total (m.m.)

Designation and no. of persons

Monthly emoluments (in Rupees)

– – – –

JRF (one) 15,600 1,87,200 (12)

1,87,200 (12)

1,87,200 (12)

5,61,600 (36)

Lab. Attendant (one)

6520 78,240 (12) 78,240 (12) 78,240 (12) 2,34,720 (36)

Total – 2,65,440 2,65,440 2,65,440 7,96,320

Justification for the manpower requirement

(i) Junior Research Fellow (One) : The experiments and estimations planned in the proposed research project will be carried out by the investigators themselves / their post-graduate students. However, to assist in the basic work like isolation of cells / DNA, preparation of reagents, sample collection and processing and data computation, a skilled JRF is required. Therefore, we propose to recruit one JRF with M.Sc./M.Tech degree in Biochemistry/Life Sciences/Medical Laboratory Technology/ Biotechnology (NET qualified) with at least one year work experience in a Biochemistry/Molecular biology laboratory and in-hand experience in molecular biology techniques; pay as per DST rules.

(ii) Laboratory attendant : The proposed project involves collection of large number of human samples. Transportation of samples from O.P.D. to lab, processing, storage, analysis, and disposal of samples, assisting tissue culture procedures like washing of plasticware and glassware, autoclaving and cleaning of lab accessories demands a full time helping hand. Therefore, we felt the need of a laboratory attendant with some work experience in laboratory work; consolidated pay as per DST rules.

Consumables

ConsumablesTo be calculated year wise as per sample size and other

variables.

• Drugs /Medicines• Chemicals• Plastic wares/ glass wares• Disposables

Justification for consumables The budget for biological reagents and chemicals constitute a major

expenditure for this project. Many of the reagents are not manufactured in India and have to be imported either directly or through their distributors. We will require reagents for

(i) Gas chromatography (GC) based analysis, that include HPLC grade solvents, high quality nitrogen gas and standerds and GC vials. ECD column used for GC can cater to approximately 300 – 400 samples. As we will process more than seven hundred samples, we will require two such ECD columns

(ii) Molecular biology work with special requirements for Real time PCR (Taqman master mix and real time tubes), reagents / kits for DNA and RNA isolation and PCR based reagents such as dNTPs, restriction enzymes, Taq polymerase for gene polymorphism studies

(iii) Cell culture. For in vitro work, two cell lines will be commercially procured.

(iv) As Real time PCR and cell culture are sensitive areas of research, we will require at least two sets of dedicated autopipettes exclusively for each area.

Plastic wares

• Autopipette : Routine use (3-5K)For small volume (7-10K)Multichannel (20K)Electronic (50K)

• Other commonly required plastic wares – ELISA plates, Microcentifuge tubes (2/1.5/0.5,0.2mL

@ 1K/1000) , Microtips (1000/100/10uL @ 1k/1000), Tip boxes (1000/100/10uL @3K/12), centrifuge tubes (15/50mL @ 1K/100 ), sample storage boxes (2/1.5/0.5/0.2mL @ 3k/12), etc.

BUDGET FOR CONSUMABLE MATERIALS BUDGET (In Rupees)

S. No. ITEM 1st year 2nd year 3rd year Total

1. Taqman master mix for Real time PCR 40,000 40,000 30,000 1,10,000

2. Taq polymerase, dNTPs, Primers, etc for PCR 35,000 35,000 20,000 90,000

3. Renal Proximal tubular epithelial cell lines 60,000 40,000 – 1,00,000

4.Cell culture reagents like FBS, RPMI, antibiotics mixtures, etc

30,000 30,000 20,000 80,000

5. DNA / RNA isolation kits / reagents 30,000 30,000 20,000 80,000

6. Glass / Plastic ware 20,000 20,000 10,000 50,000

7.Automatic pipette sets (P1000, P200, P5-50, P2-20, P2.5)

40,000 40,000 – 80,000

8. GC reagents (GC standard, Hexane, Florisil) 70,000 70,000 40,000 1,80,000

9. GC vials, Nitrogen gas 20,000 20,000 10,000 50,00010. ECD columns 50,000 90,000 – 1,40,000

TOTAL B** F***

3,95,000(US$5000)

4,15,000(US$5000)

1,50,000(US$1500)

9,60,000(US$11,500)

Travel

• Expenditure for travel include :

i. Sample collection ( particularly for survey/ epidemiologic/ multi centric sampling).

ii. Travel expenditure for presenting research work in scientific meeting ( inland only).

iii.Attending meeting for presenting annual reports to funding agency.

BUDGET FOR TRAVEL

BUDGET (In rupees)

1st year 2nd year 3rd year Total

Travel (Only inland travel) 10,000 20,000 20,000 50,000

Justification for travelThe budget for travel will be utilized for presentation of research findings in different scientific meetings and DST grant monitoring meetings.

Contingency

• Expenditure under contingency includei. Maintenance of equipmentii. Stationaryiii.Printing materialiv.Petty expenditurev. Postage

BUDGET FOR OTHER COSTS / CONTINGENCIES

BUDGET (In rupees)

1st year 2nd year 3rd year Total For equipment maintenance and contingency

10,000 20,000 20,000 50,000

Overhead• A budgetary provision by the funding authority for

infrastructural expenses to the Investigator & Project executing Institute.

• Electrical fixture, AC, refrigerator, furniture, telephone/ internet bill, common minor equipment, contingent salary for manpower assistance, gas etc.

• Calculated in terms of percentage of the total budget excluding equipments.

BUDGET ESTIMATES: SUMMARYItem BUDGET (In Rupees)

1st year 2nd year 3rd year TotalA Recurring

1. Salaries/Wages

2,65,440 2,65,440 2,65,440 7,96,320

2. Consumables 3,95,000(US$5000)

4,15,000(US$5000)

1,50,000(US$1500)

9,60,000(US$11,500)

3. Travel 10,000 20,000 20,000 50,0004. Other costs (Maintenance of equipment and contingency)

10,000 20,000 20,000 50,000

B. Equipment 22,85,000(US$42,100)

– – 22,85,000(US$42,100)

Total (A+B) FEC*

29,65,440(US$47,100)

7,20,440(US$5000)

4,55,440(US$1500)

41,41,320(US$53,600)

C. Overhead (20%) 5,93,088 1,44,088 91,088 8,28,264Grand total 35,58,528 8,64,528 5,46,528 49,69,584

Some funded projects by various funding agency

• The effect of low glycemic composite flour on diabetic and normal subjects

• Pharmacokinetic Profile of Nasal Corticosteroid and Oral antiHistaminic used in combination for treatment of Allergic Rhinitis in rural India.

• Genotype phenotype correlation in Wilson's Disease within families

• Study of New Gemostatic Gene Polymorphisms in Young Patients from North India with acute Myocardial Infraction

• Genetic, Metabolic and Hormonal Profile in Obstructive Sleep Apnea Syndrome

• Invitro inhibition of T cell Cytokines production by methotrexate: Can it predict response to methotrexate in patients with rheumatoid arthritis

• Gene-Environment interactions and susceptibility to breast cancer.

Some ICMR funded Project• MOLECULAR BASIS OF AGE-RELATED DECLINE IN LEARNING AND

MEMORY ;M.S.KANUNGO BANARAS HINDU UNIVERSITY, VARANASI

• SELENOPROTEINS AND ANTIOXIDANTS IN PATHOGENESIS OF ALZHEIMER'S DISEASE : P.RANI, P.S.G.COLLEGE OF TECHNOLOGY, COIMBATORE

• PATTERNS OF FOOD ALLERGEN- SPECIFIC CYTOKINE PRODUCTION BY T LYMPHOCYTE SUBSETS OF ATOPIC CHILDREN : B.P.CHATTERJEE INDIAN ASSOCIATION FOR CULTIVATION OF SCIENCE ,KOLKATA

• A STUDY ON THE NOVEL ANALGESIC EFFECT OF INTRASPINAL LOPERAMIDE IN A MURINE MODEL OF POSTSURGICAL PAIN : S.K.B.RAY, ALL INDIA INSTITUTE OF MEDICAL SCIENCES ,NEW DELHI

• IS OXIDATIVE STRESS AND LIPID PEROXIDATION AN VIABLE MECHANISM OF PERPETUATION OF VASCULAR PATHOLOGY DUE TO HYPERHOMOCYSTEINEMIA? SEEMA BHARGAVA, SIR GANGARAM HOSPITAL , NEW DELHI

ICMR funded project• EVALUATION OF PLATELET RECEPTOR POLYMORPHISM IN PATIENTS WITH

CORONARY ARTERY DISEASE, ENU SAXENA, ALL INDIA INSTITUTE OF MEDICAL SCIENCES , NEW DELHI

• ESTABLISHMENT OF MOLECULAR CHARACTERIZATION OF HEMOLOGLO- BINOPATHIES AND PRENATAL DIAGNOSIS OF THALASSEMIA AND SICKLE CELL DISEASE AT REGIONAL CENTRES ,M.K.GHOSH,N.R.S.MEDICAL COLLEGE AND HOSPITAL,KOLKATA

• EVALUATION OF THE BENEFICIAL EFFECTS OF TAURINE SUPPLEMENTATION IN PATIENTS WITH DIABETES MELLITUS ,S.SETHUPATHY,RAJAH MUTHIAH MEDICAL COLLEGE AND HOSPITAL, ANNAMALAI NAGAR

•

Title of the Project : Association of Common Variants of Inflammatory Cytokine Genes with Risk of Nephropathy in Type 2 Diabetes among Asian Indians

Objective : Eight single nucleotide polymorphisms (SNPs) of

pro-inflammatory cytokine genes (CCL2, TGFB1, IL8, CCR5, and MMP9) were to be genotyped.

Sample : Two independently ascertained type 2 diabetic

cohorts with nephropathy (n=200), without nephropathy (n=200) and control ( n=200).

Methodology:• DNA will be isolated by DNA isolation kit.• Genotyping will be carried out by PCR- RFLP

using a thermocycler.• hsCRP will be estimated by ELISA using

commercially available kit.( Note: Collaborative dept. have ELISA reader &

do not have a thermocycler. 50 sample DNA isolation kit costs = Rs 5 K, 96 well hsCRP kit costs Rs 12K)