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How many base pairs (BP) long is each of the fragments (bands) in “your” gel? • Pick an actual gel • Develop, justify, and use a method • Save the data “LAB REPORT”: Write a paragraph or two about this.

How many base pairs (BP) long is each of the fragments (bands) in “your” gel? Pick an actual gel Develop, justify, and use a method Save the data “LAB

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Page 1: How many base pairs (BP) long is each of the fragments (bands) in “your” gel? Pick an actual gel Develop, justify, and use a method Save the data “LAB

How many base pairs (BP) long is each of the fragments (bands) in “your”

gel?• Pick an actual gel• Develop, justify, and use a method• Save the data

“LAB REPORT”: Write a paragraph or two about this.

Page 2: How many base pairs (BP) long is each of the fragments (bands) in “your” gel? Pick an actual gel Develop, justify, and use a method Save the data “LAB

How many base pairs (BP) long is each of the fragments (bands) in “your”

gel?• Use the same actual gel• Use Loggerpro “Gel Analysis”• How do the values compare with your first set?• Which is more accurate and why?• Save the data

“LAB REPORT”: Write several paragraphs about this.

Page 3: How many base pairs (BP) long is each of the fragments (bands) in “your” gel? Pick an actual gel Develop, justify, and use a method Save the data “LAB

How do “your” results, for fragment length, compare to results from an

ideal gel.• Use the ideal gel and Loggerpro• How do the numbers compare?• Which is more accurate (what’s wrong with

ours?) and why?• Save the data

“LAB REPORT”: Write several paragraphs about this.

Page 4: How many base pairs (BP) long is each of the fragments (bands) in “your” gel? Pick an actual gel Develop, justify, and use a method Save the data “LAB

“LAB REPORT”• Introduction (hook, brief synopsis of procedures, appropriate

embellishments)• 1-2 paragraphs about the method you developed and results

(numbers!) obtained. SLIDE 1• Several paragraphs about using Loggerpro and the results

(numbers!). SLIDE 2• Several paragraphs about comparing “your” gel to the ideal gel

(numbers!). SLIDE 3• Conclusion: tie it all together (include relevance/application of gel

electrophoresis).• BONUS (the difference between an A and an A+): Explore and

explain the mathematical relationship between # of BP and migration distance (why did L-p use such a weird y axis scale?)