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HOW I DO IT Prostatic Aspiration Cytology Using Lumbar Puncture Needle PAWANINDRA LAL, MS, FRCS(ED), FRCS(GLASG),* ARUN GUPTA, MS, AND V.R. MINOCHA, MS Department of Surgery, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, India Fine-needle aspiration cytology (FNAC) is well estab- lished in the diagnosis of carcinoma of prostate. We de- scribe a simple, inexpensive method of FNAC, using a lumbar puncture (LP) needle and a disposable insulin syringe as a sheath, rather than a Franzen’s needle, which is not available at most surgical centers in developing countries. METHOD The nozzle of a disposable 1-ml insulin syringe is cut obliquely, creating a bevel (Fig. 1A). Usually the needle protrudes 10 mm from the nozzle when inserted fully inside the barrel (Fig. 1B). If the syringe is longer, the barrel has to be cut at the other end, to shorten it (Fig. 2). The plunger is reinserted into the shortened syringe bar- rel. The patient lies in the left lateral position. No anes- thesia is required. The gloved index finger of the right hand is well lubricated with jelly, and the insulin syringe is held as shown in Figure 3, with the bevel facing up and the nozzle point just short of the fingertip. The plunger may be withdrawn halfway through the syringe to in- crease the length for easy grasp. The finger holding the syringe is introduced into the rectum, and rotated ante- riorly to feel the abnormal prostatic nodule. The plunger is now completely withdrawn, while the bevel of the syringe is kept firmly pressed against the abnormal nod- ule, with the index finger. A 21-gauge metal or dispos- able LP needle with stilette is then introduced through the syringe and penetrated 5 mm into the prostatic nod- ule, feeling the grittiness of the puncture. Once the needle has entered the suspicious nodule, the finger in the rec- tum is withdrawn, allowing the syringe to hang around the needle while the needle is grasped carefully with both hands. The stilette is withdrawn and a 10-ml disposable syringe is attached to the needle. Suction is then applied to the needle while it is moved to and fro inside the *Correspondence to: Dr. Pawanindra Lal, B-90, Swasthya Vihar, Delhi-110 092, India. Fax No.: (91)-11-2432275. Accepted 30 November 1997 Fig. 1. A: The nozzle of the insulin syringe is cut along the dotted lines obliquely, as shown, creating a bevel. B: The tip of the LP needle normally protrudes 10 mm beyond the nozzle when inserted fully inside. Fig. 2. If the syringe is longer than normal, the barrel should be shortened by cutting it at the other end (dotted lines), to the required size. Fig. 3. Schematic diagram showing the grasp of the syringe in the gloved hand. Note that the nozzle is placed short of the fingertip, to permit palpation of the prostatic nodule. Journal of Surgical Oncology 1998;67:192–193 © 1998 Wiley-Liss, Inc.

How I Do It: Prostatic aspiration cytology using lumbar puncture needle

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Page 1: How I Do It: Prostatic aspiration cytology using lumbar puncture needle

HOW I DO IT

Prostatic Aspiration Cytology Using LumbarPuncture Needle

PAWANINDRA LAL, MS, FRCS(ED), FRCS(GLASG),* ARUN GUPTA, MS, AND V.R. MINOCHA, MS

Department of Surgery, University College of Medical Sciences and Guru Teg BahadurHospital, Delhi, India

Fine-needle aspiration cytology (FNAC) is well estab-lished in the diagnosis of carcinoma of prostate. We de-scribe a simple, inexpensive method of FNAC, using alumbar puncture (LP) needle and a disposable insulinsyringe as a sheath, rather than a Franzen’s needle, whichis not available at most surgical centers in developingcountries.

METHOD

The nozzle of a disposable 1-ml insulin syringe is cutobliquely, creating a bevel (Fig. 1A). Usually the needleprotrudes 10 mm from the nozzle when inserted fullyinside the barrel (Fig. 1B). If the syringe is longer, thebarrel has to be cut at the other end, to shorten it (Fig. 2).The plunger is reinserted into the shortened syringe bar-rel. The patient lies in the left lateral position. No anes-thesia is required. The gloved index finger of the righthand is well lubricated with jelly, and the insulin syringeis held as shown in Figure 3, with the bevel facing up andthe nozzle point just short of the fingertip. The plungermay be withdrawn halfway through the syringe to in-crease the length for easy grasp. The finger holding thesyringe is introduced into the rectum, and rotated ante-riorly to feel the abnormal prostatic nodule. The plungeris now completely withdrawn, while the bevel of the

syringe is kept firmly pressed against the abnormal nod-ule, with the index finger. A 21-gauge metal or dispos-able LP needle with stilette is then introduced throughthe syringe and penetrated 5 mm into the prostatic nod-ule, feeling the grittiness of the puncture. Once the needlehas entered the suspicious nodule, the finger in the rec-tum is withdrawn, allowing the syringe to hang aroundthe needle while the needle is grasped carefully with bothhands. The stilette is withdrawn and a 10-ml disposablesyringe is attached to the needle. Suction is then appliedto the needle while it is moved to and fro inside the

*Correspondence to: Dr. Pawanindra Lal, B-90, Swasthya Vihar,Delhi-110 092, India. Fax No.: (91)-11-2432275.Accepted 30 November 1997

Fig. 1. A: The nozzle of the insulin syringe is cut along the dottedlines obliquely, as shown, creating a bevel.B: The tip of the LP needlenormally protrudes 10 mm beyond the nozzle when inserted fullyinside.

Fig. 2. If the syringe is longer than normal, the barrel should beshortened by cutting it at the other end (dotted lines), to the requiredsize.

Fig. 3. Schematic diagram showing the grasp of the syringe in thegloved hand. Note that the nozzle is placed short of the fingertip, topermit palpation of the prostatic nodule.

Journal of Surgical Oncology 1998;67:192–193

© 1998 Wiley-Liss, Inc.

Page 2: How I Do It: Prostatic aspiration cytology using lumbar puncture needle

nodule. Two or three smears are made. The method offixing and staining is the same as for routine FNAC.

The insulin syringe barrel acts like a sheath protectingthe surgeon’s finger from any chance of accidental prickfrom the needle and also prevents pricking of the pa-tient’s skin or mucosa of the anorectum while it is intro-duced through the rectum. It also guides the needle tip tothe required area fairly accurately. Also, the stillette of

the LP needle prevents contamination of the needle lu-men with faeces during puncture, hence resulting in aslide free from foreign matter.

ACKNOWLEDGMENT

We thank Mrs. Sunita Samvedi, artist of our college,for her immaculate drawings.

Fine-Needle Aspiration Cytology 193