How do morphogen concentration gradients achieve in making the cerebral cortex as we know it?

What cellular responses are solely responsible by morphogen gradients?(Eliminate other possibilities such as cell-cell contact)

Holoprosencephly is a disorder characterized by the failure in development of the forebrain. In the patient’s case, the choroid plexus (secretes cerebral spinal fluid) expands and the hippocampus (consolidate long term memory) becomes smaller.

Cell Type and Shape BMP4 Gradient

Dead Cells Live Cells

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Histone-GFP mNSCs show chromatin condensation, followed a few hours later by cell membrane breakdown and PI colabeling of chromatin.

Control Chamber - Gradient Profile

Gradient Chambers - Gradient Profile

This project is supported by IM-SURE.

The PIs for this project are Dr. Edwin S. Monuki and Professor Noo Li Jeon.

Extra thanks to Jia Sheng Hu for designing the experiment and mentoring my work.

Microfluidic design accredits to Bonggeun Chung.

Thanks for the support from everyone in Jeon’s Lab.

Much appreciated Said’s effort.