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Houston Methodist Cores at a Glance Brochure
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H o u s t o n M e t h o d i s t R e s e a r c h I n s t i t u t eCoRes at a Glance
At the houston Methodist ReseARch
institute, fosteRing innovAtions with
the potentiAl foR clinicAl ApplicAtion
is At the veRy heARt of whAt we do.
integRAted into one of the nAtion’s
finest hospitAls, the ReseARch institute is
coMMitted to stReAMlining the pRocess
of tRAnslAting lAboRAtoRy ReseARch into
tReAtMents And cuRes foR ouR pAtients.
Translating laboratory discoveries into novel treatments for
patients requires specialized research and development
infrastructure, along with regulatory knowledge, technical
expertise, and specialized facilities. Houston Methodist
supports translational research with on-site capabilities
for Federal Drug Administration (FDA) approved current
good manufacturing practice (cGMP) and good laboratory
practice (GLP) studies, and early phase (I and II) clinical
trials. Our commitment to the full translational cycle from
discovery to cure is how we deliver on our mission of
leading medicine.
To support the unique technological needs inherent to conducting translational research, the
Houston Methodist Research Institute has built over 20 core facilities that provide our investigators and
external researchers access to exceptional scientific technology. Whether an investigator is in the initial
phase of discovery or is developing, testing, or validating a new drug or device, the Research Institute
has a core facility ready to assist in every step with technical expertise and state-of-the-art equipment.
ReseaRCH coresH o u s t o n M e t h o d i s t R e s e a r c h I n s t i t u t e
Translational Research Cores & ServicesTranslational research cores facilitate moving preclinical discoveries into the clinical phase of development. At the Houston Methodist Institute for Technology, Innovation & Education or MITIESM, researchers are accelerating the discovery and delivery of better care by fostering new medical technologies and techniques using advanced imaging systems, minimal access technologies, and robotics. Translational research cores also include the FDA compliant cGLP facilities to perform safety and risk assessments in preclinical models, and cGMP facilities designed to produce clinical grade radiopharmaceuticals, nanoparticles or biologicals for preclinical and clinical studies.
Research Cores & ServicesThe research cores & services provide platform technologies comprising of instruments and techniques, and services that are foundational to basic laboratory research.
Clinical Research Services & Support The clinical research cores offer clinical research infrastructure to support investigators in the design, initiation, management, and reporting of clinical trials.
Central Support Services The central support service cores provide scientific and technical expertise related to a broad range of areas such as bioinformatics, library services, scientific writing and editing, and grant application services through the Office of Strategic Research Initiatives.
Research Cores & Services
Translational Research Cores & Services
Clinical Research Services & Support
Central Support Services
010203
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2 | Houston Methodist Research Institute
Con
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translational Research cores & services
cGMP Manufacturing Core .............................................................................4
Cyclotron and Radiopharmaceutical Core ...................................................6
Translational Imaging Core ...............................................................................8
Magnetic Resonance Imaging Facility
Preclinical Imaging Facility
Positron Emission Tomography (PET) Facility
Houston Methodist Institute for Technology,
Innovation & Education (MITIESM) ................................................................ 10
Machine Shop Core ........................................................................................ 14
Comparative Medicine ................................................................................... 15
Preclinical Catheterization Laboratory Core ............................................. 16
Biorepository Core .......................................................................................... 18
Research Pathology Core ............................................................................. 19
Research cores & services
Cellular and Molecular Services
Biomicrofluidics Core ........................................................................ 20
Flow Cytometry Core ........................................................................ 22
Molecular Diagnostics Core ............................................................ 23
Nanoengineering Core ...................................................................... 24
Peptidomics Nanoengineering Core.............................................. 25
RNAcore ............................................................................................... 26
Microscopy Services
Advanced Cellular and Tissue Microscopy Core ....................... 28
Electron Microscopy Core ............................................................... 29
clinical Research services & support
Clinical Research Services ........................................................................... 30
Academic Office of Clinical Trials
Clinical Research Support ............................................................................ 32
Cockrell Centers for Advanced Therapeutics
central support services
Biomedical Informatics Services .................................................................. 34
Center Grant Proposals Support ................................................................ 35
Scientific Writing & Editing Support ........................................................... 35
Library Services ............................................................................................... 36
ReseaRCH cores
Research Cores & Services
Translational Research Cores & Services
cGMP ManufaCtuRInG core
4 | Houston Methodist Research Institute
The current Good Manufacturing Practices (cGMP) Manufacturing Core is an
FDA-approved facility that provides a controlled cleanroom and aseptic processing
environment for manufacturing molecular, nanotech, and cellular therapies.
Houston Methodist has two
cGMP cores on-site offering
investigators unparalleled
opportunities for generating
novel, clinical grade
therapeutics.
special features
•Exposedsurfacesandfloorsdesignedforrigorouscleaningandhighsanitationstandards that ensure product purity and patient safety
• HEPAfilterandairhandlingunitstoprovideparticulate-freeairatapositiveairpressure
• Continuousmonitoringofcriticalparametersliketemperature,pressure,andhumidity
• Highcapacity,ultra-purewatersystemwithenvironmentalmonitoringsystemtoassure microbiological control
• In-housewastetreatmentsystemtoneutralizewetbenchwastestreams
• Manufacturingequipmentandutilitiesvalidatedtomeetbiopharmaceuticalstandards(IQ/OQ/PQ)
• Controlledaccessfacilitywithdesignatedgowningareas,preparatorylabs,shippingand receiving stations, storage areas, and clean rooms
• Robustsampleidentification,traceabilitysystems,andelectronicdocumentcontrolsystems arepartofthecentralizedqualityinfrastructure
houstonmethodist.org/cgmpmanufacturing
Core Director: Glenn Winnier, PhDEmail: [email protected]: 713.441.2049
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•Wetbench
•RIE-1700plasmaetcher
•Waferspinrinsedryers
•Biologicalsafetycabinets(class1,000)
•CO2 incubators
•Tissueculturehoods
•Roomtemperatureandcoldstorage (4 °C, -20 °C, and -80 °C)
•Centrifugesandmicroscopes
•Stainlesssteelworksurfaces
equipment
CyCLotRon and RadIoPHaRMaCeutICaL core
6 | Houston Methodist Research Institute
The Cyclotron and Radiopharmaceutical Core is a cGMP-compliant facility that produces
clinical grade and rare custom radiopharmaceuticals for research and medical purposes.
The applications for these tracers include Positron Emission Tomography (PET) imaging
ofprostate,renal,andbraintumors,diagnosisofAlzheimer’sdiseaseandotherdementias,
locatingepilepsyseizurefocipriortosurgery,detectingcoronaryarterydisease,and
measuring the in vivo biodistribution and pharmacokinetic profiles of experimental drugs.
The on-site facility enables production
of multiple doses of PET tracers for a
patient in a single day. The half-lives
of these tracers range from 2 to110
minutes.
services
houstonmethodist.org/cyclotron
Core Director: Meixiang (Max) Yu, PhDEmail: [email protected]: 713.441.2921
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•[13N] Ammonia (Cardiac perfusion)
•[11C] Acetate (Cardiac oxygen metabolism)
•[18F]F-AV-1451(Alzheimer’sTauimaging)
•[18F]FHBG(Genetherapyimaging)
•[18F]Fluoro-L-DOPA(Braintumorimaging, over 96% sensitivity)
•[11C] Choline (Prostate cancer imaging)
•[11C]PIB(Beta-amyloidplaqueimagingfor Alzheimer’sdisease)
•[11C] Raclopride (Dopamine D2 receptor-imaging for motor and cognitive function)
•[11C]PBR28(Neuroinflammationimaging)
•[18F]GE180(Neuroinflammationimaging)
•[18F] Fluorothymidine (Cancer proliferation imaging)
•GEPETrace16.5MeVnegativeion isochronous cyclotron
•Nineventilatedhotcells
•Asepticpreparationlaminarairflowhood
•Sevenautomatedandcustomizedsynthesis modules, including a solid target system for 64Cu
•TwoClass100telemanipulatordispensing hot cells
•Nuclearpharmacyforradiopharmaceuticalspreparation and dispensing
•GMPproductionofvarious18F, 15O, 13N, 11C, and 64Cu compounds
•Integratedrapidtransportdeliverysystemto research imaging suite for short half-life isotopes
•Consultationforresearchstudiesinvolving radioisotopes
Available pet Radiopharmaceuticals Thefacilityproduces[18F] Fludeoxyglucose on a daily basis and other compounds given below on request:
equipment
tRansLatIonaL IMaGInG core
MRi facility •Housesastate-of-the-art70cmwide-bore3.0Teslawhole-bodyhumanMRIscanner
•Offerssupportforimagingresearchstudies(includingfunctionalMRI)byateamof
imagingexpertsutilizingadvancedMRIpulsesequencesandimageanalysissoftware
•Providesdedicatedareasforhumanandpreclinicalresearchwithseparateaccesstothe
scanner room
pet imaging facility •IncludesadedicatednuclearandPETimagingclinic,acyclotronfacility,anda
cGMP-compliant laboratory for the production of clinical grade radiopharmaceuticals
•Operateslatestgenerationscannersforadvancedscintigraphicimagingofshort-
and long-lived radioisotopes
•ProvidesfullsupportforInvestigationalNewDrug(IND)filing,imagingprotocol
development,andoptimizationforclinicaltrials
8 | Houston Methodist Research Institute
The Translational Imaging Core provides access to advanced high-performance Magnetic
Resonance Imaging (MRI) and Positron Emission Tomography (PET) for use with preclinical
and clinical research studies.
preclinical imaging facility •Enablesimagingofpreclinicalstudymodels
•Imagingmodalitiesavailable:PET,SPECT,CT,MRI/MRS,ultrasound,andopticalimaging
•End-usertraining(dependingontheapplication)
•Assistancewithexperimentaldesign,imageacquisition,andimageanalysis
•Troubleshootingandoptimizationofexperiments
•Ongoingconsultationandsupport
Key equipment
• 70cmwide-bore,3.0Tesla,whole-bodyhumanMRIscanner
• InveondedicatedPETsystem
• InveonmultimodalitySPECT/CTsystem
• CaliperIVIS-200system
• Maestroin vivofluorescenceimagingsystem
MRI Director: Christof Karmonik, PhDEmail: [email protected]/mri-corePhone: 713.441.7979
MRI Medical Director: Steve H. Fung, MDEmail: [email protected]
PET Director: Daniel Y. Lee, MD, PhDEmail: [email protected]/pet_facilitiesPhone: 713.441.0806
Preclinical Imaging Director: Zheng-Zheng Shi, MD, PhDEmail: [email protected] houstonmethodist.org/preclinicalimagingPhone: 713.441.9601
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Houston MetHodIst InstItute foR teCHnoLoGy, InnoVatIon & eduCatIon
The pace of technological change in medicine is unprecedented -- making it a challenge
for surgeons and proceduralists to stay abreast of the latest innovations in their fields.
Medical care and research has also become a team effort, requiring interdisciplinary
skills, rehearsal of complex interventions, and coordinated communication among multiple
teammembers.In2007,HoustonMethodistaddressedthesechallengesbybuildinga
world-leading institute for medical education and research called the Houston Methodist
Institute for Technology, Innovation & Education or MITIESM.
One of the largest and most comprehensive education and research
facilities in the world, MITIE is defining a revolutionary platform for
the ongoing training of physicians and health care providers.10|HoustonMethodistResearchInstitute
About Mitie MITIE is a comprehensive, state-of-the-art education and research institute at
Houston Methodist. It was developed to foster novel training programs in procedure-
based medicine and as a platform for research and development. In addition to a
sophisticated physical space, MITIE provides a world-class team of curriculum design
experts, event managers, and laboratory specialists to assist in the development and
implementation of any training or research program.
CoresAtAGlance|15
MITIE encompasses more than 40,000 square feet of education and research space and serves thousands of health care providers each year from all around the world.
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Four core components oF MItIesM
12|HoustonMethodistResearchInstitute
procedural skills lab for hands-on procedural training
•Fifteenoperatingroomsandendoscopysuites
•State-of-the-artmodelsforteachingproceduresinanyspecialty
•Fullcomplementofsurgicaltoolsequivalenttoclinicaloperatingrooms
Medical presence suite •Seats24;customizableviewingexperience
•PanoramicvideowallwithHDstreaming
•Real-timetwo-waycommunicationwithanyoperatingroomorprocedurearea
virtual hospital for team training in simulated patient care environments
•Simulationtechnologytorecreateanypatientcareenvironment
•Sophisticatedfull-bodyhumanpatientsimulatorsthatbreathe,blink,talk,andrespondtomedications
•Audiovisualrecordingcapabilitiesthatfacilitateteam-baseddebriefings
•Sixdebriefconferencerooms
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for an immersive viewing environment in the operating room or procedure suite
mitietexas.com
Houston Methodist Institute for Technology, Innovation & Education (MITIESM)Medical Director: Brian J. Dunkin, MD, FACSEmail: [email protected]: 713.441.7912
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experimental operating Rooms for research and development
•Twofullyequippedoperatingroomsmodifiableforanyspecialtyset-up
•Computertomography(CT)equippedoperatingroom
•MRIequippedoperatingroom
•Roboticallyguidedfluoroscopicimagingoperatingroom(HybridSuite)
4
Operations Manager: Angela MitchellEmail: [email protected]: 713.441.3998
Siemens MAGNETOM Aera 1.5 Tesla Wide Bore MRI
•For intraoperative MRI (iMRI)
Siemens Artis Zeego
Providesroboticallyguidedfluoroscopyfor:
•Traditional2DX-rayimages
•Advancedfluoroscopic(“liveX-ray”)images/video, including360-degreeX-rayview
•DynaCTforCT-likescans
•3Danatomicalreconstructions
Hansen Medical Magellan Robotic System
•Roboticallyguidedendovascularcathetersystem
Intuitive da Vinci Si HD Surgical Systems
•Forroboticallyguidedminimallyinvasivesurgery
•Equippedwithvirtualrealitysimulators(“backpacks”)forskillsrehearsal
Volcano Intravascular Ultrasound (IVUS) System
•Catheter-basedsystemthatallowsforacquisitionofultrasoundimages from within the vessel itself
Key equipment within the operating suites
procedural skills lab for hands-on procedural training
•Fifteenoperatingroomsandendoscopysuites
•State-of-the-artmodelsforteachingproceduresinanyspecialty
•Fullcomplementofsurgicaltoolsequivalenttoclinicaloperatingrooms
Medical presence suite •Seats24;customizableviewingexperience
•PanoramicvideowallwithHDstreaming
•Real-timetwo-waycommunicationwithanyoperatingroomorprocedurearea
virtual hospital for team training in simulated patient care environments
•Simulationtechnologytorecreateanypatientcareenvironment
•Sophisticatedfull-bodyhumanpatientsimulatorsthatbreathe,blink,talk,andrespondtomedications
•Audiovisualrecordingcapabilitiesthatfacilitateteam-baseddebriefings
•Sixdebriefconferencerooms
The Machine Shop develops prototypes of medical devices and other instruments
by providing concept creation, product design, and precision machining services.
A broad range of materials are available for device fabrication, including polymer,
carbon-steel, stainless steel, brass, copper, titanium, polycarbonate, peek, and delrin.
PrototypedevicesdevelopedintheMachineShopincludetheDeBakeyrotaryblood
pump, preclinical model holder, Nedo pump, and infant tongue depressor.
MaCHIne sHoP core
services •Designconsultation
•Developingprototypesformedicaldevices
•Fabricatingcustomlaboratoryequipment
•Implementingimprovementstodevicedesigns
•Devicerepair
equipment •13”Colchesterlathe •PrecisionHardingetoolroomlathe
•Kneemills •CNCBenchmanmill
•Verticalandhorizontalsaws •Shearandbrakemetalfabricator
•Tablesaw •Silversolderingcapabilities
houstonmethodist.org/machineshop
Core Director: Juan A. FernandezEmail: [email protected]: 713.441.6399
14|HoustonMethodistResearchInstitute
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The Houston Methodist Comparative Medicine program conducts studies in both
large and small preclinical models. The program has:
•65,000squarefeetofdedicatedstate-of-the-artfacilities
•Housingspaceforavarietyofpreclinicalmodels,individualventilatedcaging forrodents,andflexiblehousingconfigurationsforlargerpreclinicalmodels
•Rodentspacemanagedasastrictbarrier,specializedspaceforimaging andanABSL2isolationsuite
•TwofullyequippedoperatingroomsandanABSL3suiteforinfectiousagents
The program also offers good laboratory practices or GLP services for conducting risk,
safety, and efficacy assessment studies in compliance with current FDA guidelines.
Veterinary pathologist services and expertise for interpretation of preclinical study
data is also available.
CoMPaRatIVe MedICIne
houstonmethodist.org/comparativemedicine
Core Director: Tanya Burkholder, DVM, DACLAMEmail: [email protected]: 713.441.8685
Veterinary Pathologist: Judit Markovits, DVM, PhDEmail: [email protected]: 713.441.8404
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glp services
•WritingIACUCprotocols
•ConsultationforGLPprotocols
•Assistancefordevice-related procedures
veterinary pathology services
•Consultationonstudydesignand tissue collection for studies with pathology end points
•Reportingpathologyfindings
• Performing preclinical model procedures including injections, blood draws, catheter placements, clinical observations, equipment calibrations, monitoring, and necropsy
•Routineprocessingandslidegeneration
•Stainingwithroutineandspecialstains, including immunohistochemistry
•Necropsy
houstonmethodist.org/machineshop
PReCLInICaL CatHeteRIzatIon LaboRatoRy core
16 | Houston Methodist Research Institute
ThePreclinicalCatheterizationLaboratoryCoreoffersinvestigatorsandindustrywitha
state-of-the-art environment to train physicians, nurses, and technologists in the use of
medical devices in preclinical or inanimate models. The core is supported by the Houston
MethodistResearchInstitute’sComparativeMedicineProgram.ThecoreoffersaSeimens
AxiomArtisfluoroscopicC-armforusewithpreclinicalstudiesandphysiciantrainingfor
approved and preapproved devices.
The core personnel perform many
cardiovascular surgical procedures such
as vessel access and cath lab surgical
procedures, and have extensive
experience operating simulators
for cardiovascular surgery.
ThefluoroscopicC-armiscapableofproducingimagesknownasDyna-CT®. Transferring
these images to the Seimens Syngo®workstationallowsfortheproductionof3Dimages.
The core also has a conference room for didactic training sessions, and a large control
room for viewing procedures out of the field of radiation.
houstonmethodist.org/pccl
Core Director: Daryl SchulzEmail: [email protected]: 713.441.3318
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services
•Cardiacandperipheralinterventions •Electrophysiologystudies
•Radiofrequencyandlaserablations •Endovascularinterventions
•Roboticcatheterstudies •Pacemakerimplants
training
•Hansenroboticcatheter •Transseptalpuncture
•Cardiacandperipheralintervention •Chronictotalocclusionintervention
•Carotidstentimplantationwithdistal •Epicardialpuncture filter protection
TheBiorepositoryCoreisalarge,general-purposesamplebankofsolidtissuesamples,
blood,serum,plasmaand,otherbodyfluidsforusebyHoustonMethodistresearchersand
their collaborators. All sample collection activities are conducted in a manner that ensures
patient confidentiality, accurate pathologic annotation, and high ethical standards before,
during, and after sample collection.
bIoRePosItoRy core
houstonmethodist.org/biorepository
18|HoustonMethodistResearchInstitute
services
•Collection,processingandstorageina timely, quality driven manner of fresh, snap-frozen,solidtissuesfromsurgicalpathology specimens, and blood and otherbodyfluidsfrompatients
•Provisionofrelevantcontrolsincludingnormal solid tissues from same or other patients,and/orbloodfromhealthy patients/volunteers
•Paraffinembedding,Immunohistochemical and H&E staining, and pathology review services provided in conjunction with Research Pathology service
•Protocol-specificcollection,processing, and storage for biospecimens
•Maintenanceofaclinicaldatabasewithbasic clinical data elements for all samples in the repository
•Round-the-clockmonitoringoffreezerswith adequate backup and disaster recovery protocols in place
•Currentglobalbio-bankingstandardsfollowed to ensure research relevance of stored specimens
Core Director: Rajeev Singh, MD, MBAEmail: [email protected]
Core Manager: Pam McShaneEmail: [email protected]
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The Research Pathology Core assists with routine histology techniques such as
embedding tissue, sectioning tissue blocks, and staining sections. Custom tissue blocks
can be prepared by the core staff, including multi-tissue arrays. Immunohistochemistry
servicesofferedincludeantibodycharacterizationandoptimization,andimmunohistochemical
andimmunofluorescencestaining.Thecorehandlesbothpreclinicaltissuesamplesand
patient samples with required approvals.
ReseaRCH PatHoLoGy core
houstonmethodist.org/researchpathology
Core Director: Andreana Rivera, MDEmail: [email protected]: 713.441.2662
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services
•Specimenprocessingandembedding
•Microtomy
•Designandcreationoftissuemicroarrays
•Cryostattrainingandtechnicalsupport
•Tissuestaining(H&E,Gram,GMS,Iron, VonKossa)
•Immunohistochemistry(antibodies provided by core or investigator)
•Titration/optimizationofantibodies
•Immunofluorescence(antibodiesprovided by investigator)
•Consultationwithapathologistuponrequest
•Providesuppliese.g.cassettes,formalin, slides, molds, and slide boxes
•Rushserviceavailableforanadditionalfee
houstonmethodist.org/biorepository
TheBiomicrofluidicsCorecreatescustomdesignedmicrofluidicmoldsandchips,
fabricatespre-madebiomicrofluidicchips,andprovidesconsultationservicesinthe
biomicrofluidicsfield.
bIoMICRofLuIdICs core
services • Consultationforbuildingyourownbiomicrofluidicchips • Fabricationofthefollowing: − Replica molds used for soft lithography − Microfluidic PDMS/Glass chips − Pre-made microfluidic chips:
v-chip is a microfluidic device that quickly measures the concentration of a biomarker in a biological sample and allows for the analysis of up to 50 samples in parallel. The quantitative results are displayed directly on the device in the form of ink bar graphs. The V-chip is a cheap and easy-to-use device that does not rely on optical or electronic components.
cell separation chip has a micropost array structure and is able to separate cells
by size and deformability in a high-throughput manner. This chip is being used for
probing cellular heterogeneity and identifying more invasive
phenotypes in cancer cells.
20 | Houston Methodist Research Institute
M-chip isahigh-throughputmicrofluidicdevicewith3120ultra-miniaturizedchamberstomonitor mesenchymal-mode migration and screen for anti-metastatic drugs that specifically inhibit mesenchymal migration.ThesmallsizeofsamplesusedwiththeM-chipallowsdetectionandanalysisofthemetastatic potential of primary or rare cells.
single-cell analysis chipusesalive-cellprintingtechnique(Block-Cell-Printing)todepositsinglecellsonaPS/glasssubstrateinordertocreatefunctionalsingle-cellarrays.Thesesingle-cellarrays may be used to screen cell heterogeneity and function, including cell protrusion, cell-cell communication, and patterning.
single-cell pipette tip can transfer and deposit live single cells directly from a cell suspension to any container for single cells. The tip is compatible with the commonly used air displacement pipette (ADP). It provides for low cost, rapid, controllable, single-cell manipulation with high cell viability, high efficiency, and operational simplicity.
equipment •OAImodel200maskaligner
•Self-builtUVexposuresystem
•NikonEclipseLV100NDmicroscope
•PlasmaEtchPE50andHarrickplasmacleaners
•ThermoscientificSuper-Nuovasingle-positiondigitalstirringhotplates
•Dongdaengravingmachine
•SchmidtTechholespuncher
houstonmethodist.org/biomicrofluidics
Core Director: Lidong Qin, PhD Email: [email protected] Phone: 713.441.7328
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Core Manager: Erkuan Wang, MSEmail: [email protected]: 713.441.9633
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The Flow Cytometry Core provides investigators with state-of-the-art equipment and
supportforflowcytometricanalysisandhigh-speedcellsorting.Analyticalapplications
can be performed directly by the end user or by facility staff members. End-user training
in various types of applications, as well as software support, is also provided.
fLow CytoMetRy core
services •Experimentaldesign •Samplepreparation •Propercontrols
•Excitationanddetection •Datainterpretation •Manuscriptpreparation
equipment
•BDFACSLSRII–Fivelaserswith355,405,488,561,and630nmexcitation;14coloranalysis
•BDFACSFortessa–Fourlaserswith405,488,561,and630nmexcitation;13coloranalysis;forwardscatterPMT;FSCphotodiode
•NIRBDFACSAriall–Sixlaserswith355,405,488,550,635,and785nmexcitation; 18coloranalysis;forwardscatterPMT;FSCphotodiode;high-speedsamplesorting; temperaturecontrolledsampleandcollectionchambers;four-wayandsingle-celldeposition
•SORPBDFACSAriall–Fourlaserswith405,488,550,and635nmexcitation; 13colordetection;high-speedsamplesorting;temperaturecontrolledsampleandcollectionchambers;four-wayandsingle-celldeposition
•AMilliporeGuava8-HT–Twolaserswith488and635nmexcitation;6colordetection; 96-well handling capability
houstonmethodist.org/flowcytometry
Core Director: David L. Haviland, PhD, C. Cy.Email: [email protected]: 713.441.9233
22 | Houston Methodist Research Institute
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The Molecular Diagnostics Core provides access to advanced instrumentation
for gene expression analysis, SNP genotyping, capillary electrophoresis, and
other molecular assays. Core instrumentation is available for self-use by trained
investigators.
MoLeCuLaR dIaGnostICs core
services
•End-usertrainingfortheuseofinstrumentation
•Experimentaldesign
•Consultationandsupport
equipment
•AffymetrixGeneChip® Instrument System
− Microarray platform for gene expression analysis
− Dell Precision 490 workstation with Affymetrix software
•Aligent2100
−Microfluidics-basedplatformforcapillaryelectrophoresisofDNA,RNA,proteins,andcells
•NanoDrop®ND-1000UV-VisSpectrophotometer
− Highly accurate analysis of nucleic acid and protein concentration
houstonmethodist.org/molecular-diagnostics
Core Director: Randall J. Olsen, MD, PhDEmail: [email protected]: 713.441.6802
Core Staff: Dilzi ModyEmail: [email protected]: 713.441.1771
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houstonmethodist.org/flowcytometry
The Nanoengineering core develops and produces state-of-the-art silicon-based
bio-nanotechnology using microfabrication techniques. Scalability, precision, and
reproducibility are characteristics of the silicon microfabrication processes which
are extremely valuable for clinical applications. The core has developed a series of
silicon-based nanomedicine platforms including Porous Silicon Particles (PSPs) and
other3dimensionalnanostructures.Owingtotheirbiodegradabilityandreadilymodified
surface properties, PSPs are emerging as promising injectable drug delivery carriers.
nanoenGIneeRInG core
•Developandproducesilicon-based nanoparticles
-Routine:1μmx400nmdiscswith 30nmor60nmpores,2.5μmx700nmdiscs with 60 nm pores, and 600 nm x 400nmdiscswith30nmpores.
-Availablebyrequest:7μmx2μmdiscs, 3.2μmhemisphere,1μmx200nmdiscs,600nmx200nmdiscs,1μmx400nmdiscswithlargerpores,and2.5μmx 700nmdiscswithlargerpores
•DesignandfabricatePSPsfeaturing preciselytailoredsize,shape,andporous structure
•Developmultistagedeliverysystemcomprisingseveral nested nanocomponents, each of which is designed to negotiate one or more biological barriers
•Fabricatestage1PSPsinanon-spherical geometry to enable superior blood margination and cell surface adhesion
•Designandfabricate3dimensional nanostructures for biomedical application
services
houstonmethodist.org/nanoengineering
Core Director: Xuewu Liu, PhDEmail: [email protected]: 713.441.7322
24 | Houston Methodist Research Institute
Cores At A Glance | 25
The Peptidomics NanoEngineering Core is a facility that designs and fabricates
customizedNanoTrapsforprotein/peptidebiomarkerdiscoveryandspecificbiomarker
detection. As an active research laboratory, the core is continuously developing new
methods to improve results.
PePtIdoMICs nanoenGIneeRInG core
•NanoTrapdesignandfabrication
•On-chipsamplefractionationand data analysis
•Nanoparticleinsertion
•Peptidesandproteinsidentification
•Unknownlowmolecularweightprotein/ peptide biomarker discovery
•Targetedpeptidebiomarkerextraction and quantification (e.g. Hepcidin-25)
•BrukerMicroflexLRFMALDI-TOF mass spectrometer
•ThermoScientificVelosProiontrap mass spectrometer
•ThermoScientificDionexUltimate 3000system
•J.A.WoollamCo.M-2000VI spectroscopic ellipsometers
•BrewerScienceCee200Xautomatic spin coater
•ThermoScientificprogrammableoven
•NordsonAP-600Marchasher
•LindbergBLUEMvacuumoven
•ThermoSpeedvacSPD111Vwith RVT4104trapandOFP400pump
•FisherScientificsonicdismembrator
services
equipment
houstonmethodist.org/peptidomicsnanoengineering
Core Director: Tony Hu, PhDEmail: [email protected]: 713.441.5530
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houstonmethodist.org/nanoengineering
ThecoreservesastheRNAcorefortheProgenitorCellBiologyConsortiumofthe
NationalHeart,LungandBloodInstitute.Thecoreproducesresearchandclinicalgrade
RNA, including modified mRNA (mmRNA) of any gene of interest, microRNA cassettes
andnoncodingRNA.ThemRNA/mmRNAconstructsproducedbythiscorehavehigher
fidelity because of the use of error-free plasmid templates for in vitro transcription rather
than polymerase chain reaction (PCR)-generated templates.
Rnacore
services • Expertassistancewithplasmiddesign
• Synthesisofhighfidelityresearchandclinicalgrade:
−mRNA –modifiedmRNA(mmRNA)
− mRNA cassettes − noncoding RNA
− piRNA clusters − non-coding RNA including single-guided RNA (sgRNA)
−customizedpolycistronicmRNA/mmRNAconstructswithareportergene
26 | Houston Methodist Research Institute
houstonmethodist.org/rnacore
R&D Director: Eduard Yakubov, PhDEmail: [email protected]: 713.441.5053
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Clinical Director: Ivone Bruno, PhDEmail: [email protected]: 713.441.7292
CoresAtAGlance|27
All RNAs may be produced for human
and mouse species and are synthesized
as modified mRNAs with 5-methyl-CTP,
pseudo-UTP, bearing an anti-reverse cap
analog (ARCA) and long 150 nt poly-A tail.
RNA available from RNAcore
Reporter Proteins
eGFP Flus
nGFP b-Gal
Reprogramming Factors
OCT4 cMYC
SOX2 LIN28
KLF4 NANOG
Endothelium Lineage
FLI1 ETV2
GATA2 RUNX1c
OthersH1FOO IL12
Signaling Pathways
IL-6 STAT3
MAPK/JNK MAPK/JNK
NF-kB AP1
IFNA2 STAT1
TGF-b STAT2
PKR SMAD3
PKC SMAD4
Others
PRKACA/PRKACB CREB
RHOA NFAT
PAX7 SRF
YAP1 P53
MECP2 HSF1
SERPINA1 (AAT) HIF1A
F9 (Coagulation Factor) MBD3
DNA damage TCF3 (E2A)
Heat shock Hypoxia
Genome Editing
Recombinase Cas9
Zinc-Finger Nuclease
hCas9 D10A Nickase
TALEN dCas9-CatInact (dead)
TCR and CAR constructs
RNA IMMEDIATELY AVAILABLE RNA ON DEMAND
Lineage Factors
Beta-Cell NGN3 MAF1
PDX1 UL48 (VP16)
Blood Progenitors GATA1 GATA4
GATA3
Cardiomyocyte TBX5 MESP1
MYEF2
Hepatocytes HNF1F FOXA2
HNF4A FOXA3
FOXA1
Neuron BRN2 NEUROD1
ASCL1 NEUROD2
NGN2 LHX3
MNX1 FOXA2
ISL1 ZIC1
ASCL1 -
Osteoclast FOS TFE3
MITF
Myocytes MYOD1
RNA ON DEMAND
The core provides access to high performance microscopy for cell biology and tissue
pathology studies. The core staff will assist with experimental design, sample preparation,
advanced microscope image acquisition, and software for quantitative data analysis.
adVanCed CeLLuLaR and tIssue MICRosCoPy core
houstonmethodist.org/tissue-cellular-imaging
Core Manager: Kemi Cui, MD, PhDEmail: [email protected]: 713.441.7270
28 | Houston Methodist Research Institute
services •Brightfield,phasecontrast,differential
interference contrast (DIC), and fluorescenceimaging
•Widefieldandconfocallive-cellimaging
•Darkfieldandreflectedimaging
•Laserscanningandspinningdisk confocal
equipment •ImagestreamXimagingcytometer
high content screening system
•ImageXpressmicrohighcontent screening system
•Livecellspinningdiskconfocal imaging system
•FluoView™FV1000confocal microscope
•Multispectralimaging(Cri)
•Totalinternalreflectionfluorescence (TIRF) imaging
•Lasercapturemicrodissection
•Highcontentscreening
•2D/3Ddeconvolution
•IncuCyte™live-cellimaging– a microscope within an incubator
•Totalinternalreflectionfluorescencemicroscopy
•NikonA1confocalimagingsystem
•Lasercapturemicrodissection(LCM,Arcturus)
•Inverted/uprightfluorescentmicroscopes
•CRInuancemultispectralimagingsystem
Cores At A Glance | 29
The Electron Microscopy Core is equipped to perform scanning electron microscopy,
transmissionelectronmicroscopy,fluorescentmicroscopy,andatomicforcemicroscopy.
eLeCtRon MICRosCoPy core
equipment
•BiocatalystAtomicForceMicroscope
− High-resolution optical imaging, life science atomic force microscope
−MIROsoftwareintegratesfluorescentandatomicforcemicroscopy
− Compatible with coverslips, petri dishes, and perfusing stage incubator for live cell imaging
• MultimodeAtomicForceMicroscope
−Ultrahigh-resolutionmaterialsciencemicroscopeforimaginginairorfluidmedium
−EquippedwithScanAsyst,PeakForceQuantitativeNanomechanicalimagingmodes, and Peak Force Tapping technology
•NovaNanoSEM230
−Ultrahigh-resolutionscanningelectronmicroscopeforgeneratingnanoscale images of material and biological specimens
− High and low vacuum may be used for imaging in the presence of non-conducting and contaminating materials
− Secondary and backscattered electron in-lens detection and beam deceleration
•TransmissionElectronMicroscopes(TEM)
JeolJEM-1400Electronmicroscope
−UltraHighResolutionTEMforclinicalwork(tissueandcellularimaging)
NovaNano230SEMwithSTEMdetector
− High resolution TEM imaging function for research work (function materials, tissues and cellular imaging)
houstonmethodist.org/SEM
Core Director: Jianhua (James) Gu, PhDEmail: [email protected]: 713.441.9955
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houstonmethodist.org/tissue-cellular-imaging
CLInICaL ReseaRCH services
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Clinical Research Services
provide leadership for clinical
trials operations by providing
investigators with experienced
and certified staff to promote
innovative clinical research.
The Academic Office of Clinical Trials supports programs and physicians by providing
investigators with experienced and certified research coordinators, regulatory personnel,
and financial infrastructure to support internally and externally sponsored clinical
research.Byservingasaliaisonbetweeninvestigatorsandindustrysponsors,
the Academic Office of Clinical Trials facilitates the integration of clinical research
operations throughout Houston Methodist. The Academic Office of Clinical Trials is
also responsible for all components of the Clinical Trials Management System (CTMS)
at Houston Methodist.
30|HoustonMethodistResearchInstitute
CoresAtAGlance|31
Resources
Regulatory Support
•IRBsubmissionsandamendments
•Informedconsentpreparation
•Regulatorydocumentpreparation and management
Research Coordinator Support
•Studyfeasibilityandinitiation
•Investigatormeetings
•Subjectscreening,enrollment,andfollow-up
•Specimencollection
•IPaccountability
Financial Support
•Costassessment
•Studybudgetpreparationandnegotiation
•Invoicing
•InvestigationalNewDevices(IND)and Investigational Device Exemptions (IDE) applications
•Datacollection
•CaseRecordForm(CRF)completion
•CTMSentry
•Studycloseout
•Financialreporting
•Reconciliationofthe study cost center
houstonmethodist.org/clinical-research-services
Academic office of clinical trials Director: Pauline Todd, MBA, BSN, RNEmail: [email protected] Phone: 713.441.3242Clinical Research Services Mainline: 713.441.3250
Manager: Lenis Sosa, MSN, BSN, RN, OCNEmail: [email protected]: 713.441.3245
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Resources •Examroomsforoutpatientstudies
•Privateroomsforinfusionservices
•Semiprivateroomforinfusionservices and/orserialbloodsampling
•Fullyequippedspecimenprocessing laboratory
•Adjacentstate-of-the-artinpatientservices,including critical care monitoring
•Securestorage,preparation,anddispensingservices for investigational products with 24/7temperaturemonitoring
cockrell centers for Advanced therapeutics
The Cockrell Centers for Advanced Therapeutics promote and support clinical research operations
atHoustonMethodist.Theyoffermorethan7000sq.ft.ofclinicalresearchspacethroughout
the greater Houston area to support clinical trials closer to study participant homes or workplaces.
The Centers are staffed by research nurses, coordinators, and regulatory personnel to support
internally and externally sponsored clinical research.
CLInICaL ReseaRCH supportClinical research support at Houston Methodist enables rapid translation of
a potential treatment or technology from the preclinical stage into early
phase clinical trials. The Cockrell Centers for Advanced Therapeutics offer
sixsitesthroughouttheHoustonmetroareaandaPhase1Unitintheheart
of the Texas Medical Center (TMC) for clinical research support. The Centers
provide a full range of outpatient clinical care and study management services,
including regulatory submissions and budget management support for all phases
of clinical trials.
CoresAtAGlance|33
houstonmethodist.org/ccatcockrell centers for Advanced therapeutics clinical Research phase 1 unit Director: Eileen Dickman, PhD, MBA, CCRCEmail: [email protected]: 713.441.9777
HOUSTON METHODIST RESEARCH INSTITUTE
COCkREll CENTERS fOR ADvANCED THERApEUTICS ClINICAl RESEARCH pHASE 1 UNIT - TMC HOUSTON METHODIST HOSpITAl
SUGAR lAND HOUSTON METHODIST SUGAR lAND HOSpITAl
WEST HOUSTON HOUSTON METHODIST WEST HOSpITAl
pEARlAND HOUSTON METHODIST pRIMARy CARE GROUp
THE WOODlANDS (COMING SOON) HOUSTON METHODIST WOODlANDS HOSpITAl
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cockrell center for Advanced therapeutics
clinical Research phase 1 unit
ThePhase1UnitislocatedintheHoustonMethodistHospital’sMainSixSoutheastwing.
It is involved with early-phase and proof-of-concept clinical trials, and conducting advanced
pharmacokinetic or pharmacodynamic evaluations. The unit is conveniently located adjacent
to inpatient units for inpatient studies and to a radiology facility dedicated to clinical trials.
services •Early-phaseclinicaltrials
•Researchoutpatientservices
•Regulatoryandbudgetmanagement
•Pharmacokineticandpharmacodynamicsampling (extended hours)
•Infusionservicesforinvestigationaldrugs,biologics, and cell products
cockrell centers for Advanced therapeuticsDirector: Pauline Todd, MBA, BSN, RNEmail: [email protected] Phone: 713.441.3242
TheBiomedicalInformaticsServiceCoreofferscomputationalsupportforenhancinga
variety of scientific disciplines, including molecular biology, genetics, protein chemistry,
biochemistry, and clinical research. The core facilitates translational research by providing
expertise in bioinformatics, bioimaging, database development, image analysis, and
clinical informatics. The core services range from DNA, RNA, and protein sequence
analysis to database searching, genomic prediction, and molecular modeling.
houstonmethodist.org/biomedical-informatics
Core Director: Jon Xue, PhDEmail: [email protected]: 713.441.2577
Core Manager: Tian Ding, PhDEmail: [email protected]: 713.441.7266
34|HoustonMethodistResearchInstitute
services
•Bioinformatics–Microarray,RNAseq, Fluidigm, next-generation sequencing, and other platforms
•Dataanalysis–Genomic,transcriptomic, epigenomic, proteomic, metabolomic, clinical, and image data analysis
•Clustercomputingsolutions
•Experimentaldesignandevaluation
•Gene/target/diseaseanalysis
•Imageanddataacquisition,processing, analysis, retrieval, and management
•Machinelearning,datamining,and outcome studies
•Proposalplanning
•In vitro and in vivo quantitative imaging
•Statisticalanalysis–Samplesizeandpowerestimation, basic and advanced analyses
•Webportalanddatabaseprogramming
bIoMedICaL InfoRMatICs seRVICe core
houstonmethodist.org/biomedical-informatics
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center grant proposals support
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services •Developandsubmitcentergrant
proposals
•Budgetdevelopmentforcenter grant proposals
•Guideinvestigatorsthroughtheadministrativeprocesses and requirements for grants and contracts, technology transfer and intellectual property, regulatory compliance and research protections, and clinical trial services
The Office of Strategic Research Initiatives (OSRI) increases institutional competitiveness
for large grant opportunities. The office staff work directly with investigators throughout
Houston Methodist to develop multi-institutional extramural funding proposals.
otHer suPPoRt seRVICes
Manager:HanhH.Hoang,PhD|Email:[email protected]|Phone:713.441.8227
Houston Methodist researchers, clinicians, and staff have on-site access to a fee-for-service
scientificediting,writing,andconsultingsupportthroughBioScienceWriters,LLC.
Toassistwiththegrowingpressurestopublishandsecurefunding,BioScienceWriters
offers a variety of concierge services to meet the needs of native and non-native English
speaking scientists.
services •Consultingonlargegrants
•Academic/medicalwritingandediting
•Overlappingtextdetection
•Languagetranslation
•Journalsubmission
•Writingworkshops
Email: [email protected]
scientific writing & editing support
otHer suPPoRt seRVICes
The Houston Methodist Library serves as a rich resource for meeting the requested and
anticipated information needs of faculty, researchers, residents, fellows, students and other
affiliated health care professionals. In addition to the subscription to more than 9000 books
and journals and access to over 40 publication databases, the library offers access to:
36|HoustonMethodistResearchInstitute
clinical trial and Methodology databases
funding databases
trainings & workshops
library services
•CochraneCentralRegisterof Controlled Trials
•SciValFunding
•IntroductiontoElectronicMaterials
•AssemblingtheRelevantLiterature
•CochraneMethodologyRegister
•RefWorks
Hospital Librarian: Linné Girouard, MLIS, AHIP
Email:[email protected]|Phone:713.441.2229
scientific literature databases & Reference Management software
•Cochrane-Databaseof Abstracts of Reviews of Effects
•CochraneDatabaseof Systematic Reviews
•Cochrane-Health Technology Assessments
•CochraneNHSEconomic Evaluation Database
•IEEEXplore
•WebofScience
•HealthBusinessElite
•Scopus
•Medline
•PubMed
•RefWorks
•Browzine
Houston Methodist Research Institute
6670 Bertner Street
Houston, TX 77030
houstonmethodist.org/research
HMRI Office of Communications & External Relations | HMRI-101 | MM | DH | 05.2015