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food microbiology

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  • 9/10/2015 Thedetectionandidentificationofbacteria

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    Page1

    Moulds

    Page2

    Mouldshavealotof

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    Mostproblemsarecaused:

    Penicillum

    Aspergillus

    Fusarium

    mycotoxinsproducers

    Page3

    Moulds

    Reproductiondependsprimarilyonthewater

    availability

    Bacteria>0.91

    Mouldsalsofrom0.85to0.9

    SomeEurotium0.75to0.8,andAspergillus

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    Thefoodismicrobiologicallystablewhenthewater

    activityis

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    arenotPROTEIN

    arenotenzymes

    arelowmolecularweight

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    Allstrainsofthesamespeciesdonotproduce

    Differentspeciescanproducethesamemycotoxin

    Page9

    Wheremycotoxins

    produced

    Worldgrainproductionby2530%hasbeen

    abreedinggroundformoldsandseen

    Mycotoxins

    Page10

    Tuohoutuukomycotoxins

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    easily?

    Not

    Mycotoxinsusuallydonotdisappearor

    inactivatedinthemanufactureoffood

    Page11Page12

    Cerealalreadyinfectedinthecorn

    Mouldsgrowparasites,epiphytes

    orsaprophytes

    Homeplantassociationveryspecific

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    Page13

    Thecontextofpostharvestgrain

    maybedamaged

    Mushroomscanpenetrate

    thedamagedsectionjyvn

    Page14

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    Inorderforfungalgrowthwouldbeimpeded

    cerealsafterpuimisen

    dried

    Dryinglessthan14%watercontent

    Whenthegrainwatercontentislessthan5.13%it

    themicrobiologicalshelflife

    Oilbearingcropseedsmustbedriedbelow

    12.5%

    Page15

    Ifthegrainisstoredtoo

    humid

    Mouldsandfungigrow

    Metabolismproducesheatandtheharvestheatsup

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    Mushroomskilltheembryoandseednolongergerminate

    Page16

    Ifthegrainisstoredtoo

    inahumidatmosphereof6590%

    itgrowsvarastohomeita

    Eurotium,Aspergillus,Penicillum

    Later,Mucor,Rhizopus,and

    Nigraspora

    Cropqualityispooriftheconcentrationofsporesoffungal

    morethan10 6 /g

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    Page17

    Grainstorage

    Relativehumidity

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    Cerealiswettedandlesskserofyyttisetsienetkin

    cangrow

    Aspergilluscandidus,A.flavus,A.parasiticus,A.

    fumigatus,Emerciellanidulans,Penicillum

    Piceum,P.capsulatum

    Page19

    Thesefungiform

    mycelium

    Canbindgrainklimpeiksi

    orsolidlumps

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    Page20Growthandtemperature

    Page21Thegrowthandactivityofthewater

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    Page22Page23Page24Page25Page26

    FAOandtheWHOrecommendation

    max30gaflatoxin/kgfood

    timehigh,butmalnutritionversus

    livercancer

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    Page27Page28Page29

    AflatoxinB1

    LD50

    dogs0.5mg/kgbodyweight

    LD50

    rat9mg/kg

    ForratsandrainbowtroutaflatoxinB1inone

    themostcarcinogeniccompounds

    Page30

    AflatoxinB1

    India19741000peoplebecameill

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    aftereatingmoldyrice

    100died

    Page31Page32Page33Page34

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    Page35

    OchratoxinA

    WasfirstisolatedA.ochraceusSouth

    Africa

    Themoststudiedbarley,wherePverrucosum

    toproduceit

    Page36

    OchratoxinA

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    Inpigs,causedbykidneyproblems

    Movestoaccompanymeat

    Peoplewhoeatcannedalongtime

    homekypsennettyjhamsmaybeexposed

    (Balkanendemicnephropathy)

    nephrotoxicandmaycauserenalcellcarcinoma

    Page37Page38

    Sterigmatokystiini

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    Page39

    Sterigamtokystiini

    Aflatoksiinisynteesinprecursor

    ManyofAspergillusmoldsproduceit

    especiallyA.versicolor

    Notastoxicandcarcinogenicthan

    Aflatoxin

    Itisfoundinmanyfood

    ForalongtimestoredinEdamandGoudacheese

    Thepoisononlyinthesurfacelayers

    Page40

    Patulin

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    Page41

    Patulin

    PenicilliumandAspergillusproducers,including

    Byssochlamys

    Apples,blueberries,strawberries,currants,

    gooseberries

    Theeffectsofpatulinisnotknownexactly

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    Nordiccountries,themaximumallowableamountis

    50g/kg

    Page42

    Patulin

    Discoveredin1942,itwasthoughtthatitwouldbe

    goodantibioticbecauseofitslarge

    Theantimicrobialactivityof

    In1959itwasassociatedwithcattlepoisoning

    moldygerminatedbarleygrain

    Page43

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    Patulin

    Notverystable,exceptinacidic

    conditionssuchasapplejuice

    Page44Page45Page46

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    Page47

    T2

    DermonecrosiscattleintheUSA

    MaybeinRussia194247disease

    "AlimentaryToxicAleukia"

    Page48

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    T2

    immunosuppressiveagentssuchasochratoxin

    andaflatoxin

    allthreealsoinhibit

    proteinsynthesis,butatdifferentpoints

    Mixturesmaybesynergistic

    Page49Page50

    DON

    Deoksynivalenol(=vomitoxin)

    Japan"redmouthdisease",diarrheaand

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    vomit

    F.graminearum

    FeedfactorrequiredforrefusalinpigsinNorthCarolina

    LesstoxicthanT2

    Page51

    Verrukariinit

    Stachybotrysatra

    Inhorses,aftereatingmoldyhay

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    Page52Page53Page54

    Zearalenone

    vulvovaginitispigswhoatethemold

    maize

    infertility

    fewerchicks

    insomecountriesareusedasanabolics

    increaseingrowthfactorsandinothersitisforbidden

    monitoringtheproblem,becauseifthefeedisitso

    Itisalsodetectableintheflesh

    Page55Page56Page57Page58

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    Ergotamine

    Page59Page60Page61

    Ergotism

    alsoreferredtoasStAnthonyfire

    Thereisageneral

    toxiceffectspoorlyunderstood

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    Page62Page63