Laboratory Conference

Group II

Atienza, Abegail

Comia, Ralph Joseph

Castillon, Kristine

Expt. C : HMP on SMA

• Herbal Plant: Makahiya 30%

• Preparation: Decoction

• Procedure: SD 30% = 30gm/100ml = 300mg/1ml 30 grams of Makahiya 100 ml of water Boil up to 100C RD= 5mg/gm

• Animal: 5 albino mice Weight: 1-5 mice: 20 grams

20 gm 100mg x 1mL = 0.33mL

X 5mg/gm 300mg

100 mg

• Observation/ Parameters

• (F) FREQUENCY No. of movements/ minute Actual count/ min (ave.)

(A) AMPLITUDE Force of movement/ minute (+) slow (++) moderate (+++) strong

Procedure

• Place the 5 albino mice on the pool• Observe and acclimatize for 30 minutes before

the administration of drugs (Frequency and Amplitude)

• Administer the drug intraperitoneally• Observe every 15 minutes thereafter.

Mice Baseline 15 mins after 30 mins. After 45 mins. After 60 mins. After

Frequency

Amplitude

Frequency

Amplitude

Frequency

Amplitude

Frequency

Amplitude

Frequency

Amplitude

Ave 35 +++ 30 ++ 28 ++ 25 + 15 +

Result

Discussion

• Makahiya(scientific name: Mimosa pudica)

• creeping annual or perennial herb

• the compound leaves fold inward and droop when touched, re-opening within minutes.

• Common weed widely distributed in the Philippines, in open moist areas, open grasslands and open thickets

Constituents• Seed contains a toxic alkaloid, mimosine, a

non-protein alpha-amino acid, known to cause hair loss and depressed growth in mammals

• Roots yield flavonoids, phytosterol, alkaloids, amino acids, tannins, glycoside, fatty acids.

• Leaf extract have yielded an adrenaline-like substance.Seeds contain a mucilage composed of d-xylose and d-glucoronic acid, yielding 17% greenish yellow fatty oil.

Medicinal Properties

• Asthma; expectorant

• Antidepressant

• Root is aphrodisiac

• Analgesic

• Antispasmodic

• Leaves are used for diabetes

• In India, it is used as antifertility agent

• Mild sedative property

• Sedative drugs reduces anxiety (anxiolytic) and exert calming effect.

• Acts like Benzodiazepines, Barbiturates (Sedative- Hypnotic drugs)

NEUROPHARMACOLOGY

• GABA- major inhibitory neurotransmitter

• Potentiate GABAergic inhibition at all levels of the neuraxis, enhance GABA’s effects by increasing the frequency of channel-opening events ( Cl ion channels)

Effects of Sedative- Hypnotic Drugs

• Sedative drugs accompanied by depressant effects on cognitive and psychomotor functions

• inhibiting the development and spread of epileptiform electrical activity in the central nervous system (Anti- convulsant)

• Muscle relaxant- anti- spasmodic

• Conclusion

Makahiya is a delayed long-acting depressant effect

Causing mild sedation and anti- depressant.

Expt. E: HMP ON BLOOD SUGAR

• 10% Glucose- SD

• R.D 0.1mL

• Animal: albino mice

Procedure

• Starved and acclimatized the mice. Do not stress the mice

• Determine the base line blood glucose (Glucometer method)

• Inject 0.1ml 10% glucose intraperitonealy after 15min.

• Gather the blood glucose after 30min every 15min for 60min.

Result

Baseline 15 mins after

30 mins. After

45 mins. After

60 mins. after

0.3 mmol/L 0.6 mmol/L

2.5 mmol/L

2.0 mmol/L

2.5 mmol/L

Glucose

• It is a monosaccharide, and is the most important carbohydrate in biology as a source of energy and metabolic intermediate

• Glucose is an important metabolic intermediate of energy.

• It is the treatment for hypoglycemia, or low blood sugar level, by providing the glucose needed in the blood.

• Conclusion

Glucose has a sustained short- acting Hyperglycemic effects

10% Glucose- Hypertonic solution