high value products from microalgae… with economic relevance...high value products 8th Edition of the Les Rendez-vous de Concarneau • September, 29 th and 30 th 2016" Where Industry

high value products

8th Edition of the Les Rendez-vous de Concarneau• September, 29th and 30th 2016

" Where Industry meets Science"

high value products from microalgae … with

economic relevance

Vítor Verdelho VieiraPresident - European Algae Biomass AssociationMember for the Board - A4F Algae for Future

What is ‘ high value ’ ?

What is ‘economic relevance’ ?

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What is the microalgae…

…value framework ?

1. Value depends on Composition

Polysacharides(starch, glucose,

sugars)

Essentialaminoacids pattern

similar to food

Pigments(chlorophyll,

carotenoids andastaxanthin )

Microalgae

Proteins Carbohydrates

50% 20-30%

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Essential vitamins (A, B1, B2, B6, B12, C, E, nicotinate, biotin, folic acid and pantothenic

acid)

PUFAs (ARA, EPA, DHA)

astaxanthin )

Microalgae

Lipids Othercompounds

20-30% < 5%

2. Value depends on Applications

• Food ingredients

• Healthfoods

Food

• Premix feeds

• Specialty feeds

Feed

• Nutraceuticals

• Pharmaceuticals

Health

• Cosmeceuticals

• Thalassotherapy

Cosmetics

Current applications

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• Biofuels

• CO2 mitigation

Fuels

• Biofertilizer

• Soil microalgae

Fertilizers

• N&P removal

• Bioremediation

Wastewater

• Biofibers

• Chemical industry

Chemicals

Emerging applications

3. Value depends on Purity Level

Increasingly Market driven

Current product demand

Aquaculture

Paste

Food & Feed

Aquaculture

Dried

Ceuticals

Extracts

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demand

New Products

BiorefineryConcept

Ceuticals

The products from microalgae have currently only three possible forms

4. Value depends on Formulation

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Only 3 possibilities:

PASTE DRIED EXTRACTS

Mostly for: Aquaculture Food & Feed Ceuticals

usually from 5 to 15% Dry Weight with different packaging

spray-dried,freeze-dried or just sun-dried

solvents, super-critically extracts or just with

mechanical processes

Value-chain analysis is a complex topic very often mentionedby non-experts that bring a highly confusing, misleading andunclear approach.

The 'value-chain' is defined in a business context as:" the interrelated operating activities businesses

5. Value depends on ‘value-chain’ Position

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" the interrelated operating activities businessesperform during the process of converting rawmaterials into finished products" .

The concept comes from business management and was first described and popularized by Michael Porterin his 1985 best-seller (Competitive Advantage: Creating and Sustaining Superior Performance).

Business value chain Product value chain

> Value-chain for microalgae biomass

PRODUCTION PROCESSING DISTRIBUTION

PRIMARY

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SUPPORT

PRODUCTS

APPLICATIONS

> Value-chain for microalgae based products

FORMULATION DISTRIBUTION

PRIMARY

The global microalgae value chainfor any microalgae or microalgaebased product can be quite simple

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SUPPORT

PRODUCTS

APPLICATIONS

based product can be quite simpleor complex. Different steps wherevalue is added along the processof transforming a biologicalmaterial in a product for a client oreven a consumer.

Different products can come-outin different points of the value-chain and they can be tradedforward to the next point of thechain or further to other point.

Packaging defines a product thatcan be traded.

Value chain and market values - examples

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Values for 2012 under updating

6. Value depends also on production Scale

SMALL SCALESMALL SCALE MEDIUM SCALEMEDIUM SCALE

< 5 haproduction: 10-100 ton /year

more than 500production facilities all over the world

5-50 haproduction: 100-500 ton/year

less than 300 production facilities mostly in China and other Asian countries

LARGE SCALELARGE SCALE

50-100 haproduction: 1000-5000 ton/y

less than 20 Spirulina production facilities with this scale

VERY LARGE SCALEVERY LARGE SCALE

> 100 ha > 5.000 ton /year

still no production facility with this sizeO

PE

N S

YS

TE

M

SU

RFA

CE

11/43LARGE SCALE DEPENDS ON HOW WE COMPARE WITH REFERENC ES

countries

CLO

SE

D S

YS

TE

M < 10 m3 -

production: < 1 ton/year

more than 100production facilities all over the world

SMALL SCALESMALL SCALE MEDIUM SCALEMEDIUM SCALE LARGE SCALELARGE SCALE VERY LARGE SCALEVERY LARGE SCALE

10-500 m3 production: 1-50 ton/year

less than 50production facilities all over the world

500-2.000 m3 production: 25-150 ton/year –

less than 10only 4 with more than 1.000 m3 (BGG, Algatech, Buggypower, Allmicroalgae)

> 2.000 m3

> 50-70 ton /year

still no production facility with this size

VO

LUM

E

there is also a very small scale > the microfarming with less than 0.5 ha

7. Value depends also on comparative reference

Soy: oil & meal Fish: oil & meal Algae: oil & meal

> 200 million ton/year of

production

> 7 million ton/year of

production

> 20 thousand ton/year of

production

Feed applications are the

most relevant

Feed applications are the

most relevant

Food applications are the

most relevant

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most relevant most relevant most relevant

Soy based feeds improved

with fish meal

Fish meal feeds are improved

with algae

Algae emerge as pre-mix feed

ingredient

Current value < 0.5 €

typically 0.35 €/Kg

Current value < 2 €

typically 1.5 €/Kg

Current value < 10 €

typically 10-20 €/kg

10 €/Kg is a high value

… when compared with 0.35€/Kg related oil products

Value chain and market values

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Values for 2012 – under updating



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Same examples

Aquaculture hatchery feeds

Aquarium feeds Premix feeds for farm animal

Specialty feeds for racing animal

Food ingredients for pet foods

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Agriculture extracts

Food ingredients Food supplements Nutraceuticals and health food

Cosmetics and cosmeceutical

Aquaculture hatchery feeds

www.tomalgae.comNannochloropsisoculata is the main cultured organism contained in PhytoBloom .

Nannochloropsis .

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www.phytobloom.com

www.brineshrimpdirect.com

http://reedmariculture.com/application_finfish.php

Nannochloropsis . Isochrysis

$47,25$75,00

AQUARIUM FEEDS

www.coralculture.co.uk/www.phytobloom.com

www.algaenergy.es/

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$29.99

www.petworldshop.com

www.phytobloom.com

www.dobbies.com/

www.bassleer.com

Chlorella

Spirulina

Farm animals premix feeds

Spirulina platensis, Haematococcus pluvialis, Chlorella sp, Chlorella protothecoides, Nannochloropsis sp, Chlorella sorokiniana,

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www.algaeinstitute.comwww.dhagold.com

DHA omega-3

www.tractorsupply.com/content_landing-page_dumor

www.actsinc.biz/algavit.html

Pet foods

www.watershed.net

Chlorella

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www.sunchlorellausa.com

www.livelypets.com

www.watershed.net

DHA omega-3

Racing animal speciality feeds

www.nouvelleresearch.com

Spirulina

DHA omega-3

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www.springtimeinc.com/product/longevity/All-Natural-Dog-Supplements

www.myshopping.com.au

www.dhagold.com

ww.algosud.com

Food ingredients

SPIRULINA

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http://elkenenstek.blogspot.pt/2013/10/elken-everyday.html

www.leebiscuits.com/pro03_filled_crackers.html

www.alibaba.com/product-detail/Spirulina-Filled-Crackers_160267840.htmlhttp://elkenenstek.blogspot.pt/2013/10/elken-everyday.html

http://m.shopclues.com/enerva-spirulina-cookies-2.html

Food ingredients

CHLORELLA AND SPIRULINA INGREDIENTS

€ 20,95

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www.emmaandtom.com www.jugojuice.com

http://www.bluebio.cn/

www.organo.dxneurope.eu/products

www.nl.nutress.eu

€42,40

http://www.mattisson.nl/shop/superfoods/absolute_supersmoothie_slimming_mix_bio_raw/47

Food ingredients

CHLORELLA AND SPIRULINA INGREDIENTS

www.algaeindustrymagazine.com Spirulina honey

€ 2,89

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www.spirulina.sg/Spirulina_Green_Tea.html

Spirulina contains 10-20% phycocyanin making it the best natural blue color for foods.

www.spirulina.sg/Spirulina_Honey.html

Food ingredients

SPIRULINA COLOR INGREDIENT

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Net weight: 500 g.Origin: Spain

3,15€www.spirulina.sg/Spirulina_Beer.html

www.microlife.bio

48,00€

Food supplements

SPIRULINA + CHLORELLA + DUNALIELLA

150g Powder - $82.95 / 71.51€

60 Count Bottle - $39.95 / 34.44€

$49,95 / 37,80€.

www.dr-peterhartig.de/power-22 www.e21usa.com

www.agmfoods.com

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49,99€150g Powder - $82.95 / 71.51€ $49,95 / 37,80€.

Powder: $49,95 / 37,80€Caps (each 596 mg): $61,55 / 46,60

29.99 / 25.85€360 capsules: $ 44,95 / 31,18€267g: $34,95 / 24,23€

240g: $25,95 / 17,99€

www.nowfoods.com

www.nourishinghub.com.au

www.greensplus.com

Food supplements

498.56€

DUNALIELLA

$26,42 / 18,64€

www.nikken-miho.com/index_ecat.php?cPath=2www.ethicalnutrients.com.au

www.earthalchemi.com.au/product/algotene-red-marine-phytoplankton-60-cap/

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303.98 €

$20,01 / 15,11€

60 caps: $28,90 / 21,84€; 180 caps: $69,30 / 52,37€

$26,42 / 18,64€

www.thompsonsnutrition.com.au

Food supplements

SPIRULINA

$ 22.55

£21.59/ 907g

33/43$45,30 / 31,96€

CHLORELLA + DUNALIELLA

5 oz (142g): 12 - $39,95 / 28,20€2.5 oz (354g): 30 - $68,95 / 48,68

www.sunchlorella.com

www.hollandandbarrett.com/pages/product_detail.asp?pid=822&MCatID=5&prodid=882&cid=47

ASTAXANTHIN

www.astasupreme.co.nz

Price range: $49.90 -$59.90

Nutraceuticals and health food

product with 30% = 300 US$ =>

1000 US$/ Kg 100% equivalent

PHYCOCYANINwww.euglena.jp/en/

www.earthrise.com

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50% BETAGLUCAN = 25-35 US$/Kg

1000 US$/ Kg 100% equivalent

15 € the 100 g pack

SPIRULINA

www.algalscientific.com

www.algaenergy.es

Nutraceuticals and health food

www.dsm.com

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Cosmetics and cosmeceuticals

Cellular Swiss Ice Crystal Advanced Marine Biology Repairwear Uplifting Firming Creamwww.laprairieswitzerland.com www.clinique.com

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www.ahava.com

€24.95 - 250 ml

Spirulina

17,95 €

www.naturespirit.de

£7.69

www.aubreyorganicsuk.co.uk

Blue Green Algae

www.algaenergy.es

Chlorella vulgarisIsochrysis galbana (T-ISO)


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www.mibellebiochemistry.com


It was not until 2010 that the Salins group began to study the microorganisms on the Salins. The expert scientific team in valuation of microalgae will examine the case of Dunaliella salina , a species of salt-tolerant green algae (iewho copes well with high salt concentration environments)

www.salins.com www.spiruline-des-iles-dor.com

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www.eclae.com

Price range: 14€ - 65€

Agriculture growth promoters and fertilizers

SPIRULINA

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www.algaenergy.es/

www.biorizon.es/Bioalgal_Marine/Algafert_Abonos_es.html

A4F – ALGAE FOR FUTURE

40

A4F – ALGAE FOR FUTURE September 2016

A4F AlgaFuel SA was

established in 2008 and is

dedicated to Design, Build,

Operate and Transfer

(DBOT) of large-scale

microalgae plants.

A4F has a very close relationship with the leading scientists and researchers in the field of

microalgae biotechnologies, as well as with the largest microalgae producers, worldwide.

Therefore, A4F is a first choice for any large-scale contract as the best

specialist company in almost every specific aspects of microalgae

production with the most extended knowledge network.

41

As a global company, A4F collaborate

in R&D European projects and offer

our consultancy services in Europe

(Portugal, Spain, France, UK, the

Netherlands, Germany), in the Middle

East (Saudi Arabia), South America,

and wherever our clients are.

While being a company involved in many activities within microalgae cultivation,

both in R&D and in implementation of industrial production units, A4F also helps

the clients in the development of the final products, including the biorefinery

concept.

42

Program Acronym Topic Start date Duration

GIAVAPGenetic modification of marine or freshwater

algae to better suit industrial applications2010Nov 36 months

BIOFAT Biofuels from microalgae 2011Apr 48 months

ALGAPLEX Mixotrophic microalgae growth 2009Oct 24 months

DEMA Direct bioethanol from microalgae 2012Dez 54 months

R&D PROJECTS

PHOTO.COMMPhotosynthetic communities in industrial

cultivation2012Oct 48 months

ProEcoWine Plant protection products from microalgae 2012Nov 24 months

D-Factory Cultivation and biorefinery for Dunaliella 2013Dec 48 months

PUFAChainDeveloping a pipeline of PUFA-derived products

from several species of microalgae2013Nov 48 months

ALFF The Algal Microbiome Friends and Foes 2014Dec 48 months

PHOTOFUELStimulating the innovation potential of SMEs for

a low carbon energy system. 2015Jul 48 months

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LISBON EXPERIMENTAL UNIT: SERVICES

• Production conditions optimization of freshwater and marine microalgae

• Chlorella, Haematococcus, Nannochloropsis, Dunaliella, Phaeodactylum,

Spirulina, Synechocystis, Thalasiossira, Chlamydomonas ….

• GMO and non-GMO

• Water and nutritive medium management/optimization

• Culture medium treatment and recycling optimization

• Cultivation systems custom engineering

44

• Cultivation systems custom engineering

• Biomass harvesting and processing

• Training in PBR, GW and CRW operation

Inputs + Management �� Production �� Outputs

S01

Water treatment

and distribution

S11

Offices S04

Laboratory S08

Harvesting

S02

Nutritive media

distribution

S12

Data acquisition and

control

S05

Inoculum S09

Processing

S03

Carbon delivery S06

Production PBR

S10

Effluents and

water recycling

S07

Thermoregulation

Wastewater management

LISBON EXPERIMENTAL UNIT: SYSTEMS & LAYOUT

45

S06. PBR

S12

Utilies

Unit implantation layout and systems description.

S01S03 S10

S08&

S09

S07

S11

Water recycling

LISBON EXPERIMENTAL UNIT: GMO

• Greenhouse to confine the PBR production area;

• GH and processing area ground was made impervious;

• Wastewater piping from GH and processing areas is collected in gutters connected

to a closed system into a 10 m3 decontamination tank with an exterior pipe

container allowing detection of leakages;

• Closed system communication between GH and processing area.

46Unit implantation layout and systems description.

LISBON

INNOVATION INNOVATION

LABORATORY

LIL

47

Mission

• A4F’s LIL is fully equipped to support R&D

activities. It houses A4F’s microalgae culture

collection, culture collection bank (backups

and inocula improvement programs for

production units) and hosts R&D experimental

projects and analysis in supporting pilot scale

and industrial production units.

Research activities

LISBON INNOVATION LABORATORY

48

Research activities

• Cultivation and Scale-up (GMO certification)

• Analytical Biochemistry

• Molecular Biology

• Bright field and Fluorescence Microscopy

LISBON INNOVATION LABORATORY

Culture collection

• A4F´s Culture Collection includes close to 100 strains

of microalgae and cyanobacteria on site and privileged

access to more than 1200 isolates through

partnerships and contracts.

• Microalgae are isolated from sites of interest or

acquired for clients, R&D projects, biotechnology

development.development.

49

SERVICES PROVIDED BY A4F

50

Consultancy services

• Business case study for microalgae production

• Assessment of sites for microalgae production plants

• Design of microalgae production plants

• Technology procurement and selection – infrastructure, production equipment,


51

• Technology procurement and selection – infrastructure, production equipment,

downstream processing and utilities for microalgae production plants

• Start-up, diagnosis and optimization of microalgae production plants

• Production supervision, team training and technology transfer at Clients’

microalgae production plants

• Funding opportunities for microalgae projects

• Support in sales, development and licensing of novel microalgal products

Technology supply

• Photobioreactors – mainly tubular and flat-panel, other options possible

according to suitability for different projects

• Open ponds - cascade and conventional raceways, other options possible

according to suitability for different projects

Fermentation reactors for microalgae production – his is in partnership with a


52

• Fermentation reactors for microalgae production – his is in partnership with a

specialist company in yeast and bacteria fermentors.

• Implementation of complete microalgae production plants: from cell to tons

• Development of a complete product application platform: from tons to market

• Integration of microalgae production with existing industrial facilities (cement,

power, effluent treatment, fertilizer, etc.)


Technical services

• Microalgae strain isolation, selection and characterization

• Inoculum production

• Specialized analytical services: pigments, fatty acids, elemental analysis, etc

• Growth medium design, supply (for lab and industrial scale) and optimization

53

• Growth medium design, supply (for lab and industrial scale) and optimization

for photo, mixo and heterotrophic growth

• Small group training in microalgae cultivation at A4F premises

• Contract research and development – at lab scale and pilot scale – strain

optimization, contamination control tools, biomass enrichment, etc

• Supply of pilot scale (kilogram) amounts of microalgae biomass

At the end of this stage it was possible to:

• External market study of microalgae for food and feed applications

• Develop a detailed and final Business Case for the industrial production unit

• Industrial production unit engineering design and construction

ALGAFARM PILOT

54

ALGAFARM

ALGAFARM is the world’s largest unit of microalgae production with closed tubular photobioreactors – 1300m3 of Culture

55

18th-22nd November 2013

LiMBAC

2nd Edition will give a larger emphasis to pilot pl ant cultivation techniques

13th – 18th November 2016

SAVE DATESAVE DATE

Venue: A4F, Algae For Future, SACampus do Lumiar - Edifício E, r/c Estrada do Paço do Lumiar 1049-038 Lisboa, PortugalPhone: +351 218072499Website: www.a4f.ptMore information: [email protected] and RegistrationThe course fee is 800 € (for students) and 1600€ (for non-students)It includes the course and all supporting material, daily commutes between A4F and hotel, coffeebreaks, lunches and one conference dinnerAccommodationDiscount rates for hotel accommodation will be announced shortly.

1st edition LIMBAC - Lisbon, in 2013:• Attracted 50 participants from 23 countries, with an average age of 32;• A very interactive programme, participants from 18 companies and 17 Universities;• Focused largely on lectures about case studies of microalgae businesses;• Practical Lab sessions about isolation, cultivation;• Biochemical analysis of microalgae cultures.

2nd edition LiMBAC

Lisbon, 13 th to 18 th November 2016

The 2nd edition of the LIMBAC aims tofamiliarize participants with advancedconcepts of microalgae cultivation with aspecial attention to scale-up processes, largescale cultivation and downstream processing.The course will take an interdisciplinaryapproach, presenting lectures covering thewhole microalgae value chain as well as laband pilot plant practical sections whereparticipants will have hands-on experience inculturing microalgae. The course is targetedto participants with some background andexperience in the microalgae field.

18th-22nd November 2013

[email protected]

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Vítor Verdelho with special thanks to Joana Lapa This presentation is the confidential work product of A4F AlgaFuel SA and no portion of this

presentation may be copied, published, performed, or redistributed without the express

written authority of a A4F Officer. © Copyright 2016, A4F AlgaFuel SA

Documents

high value products from microalgae… with economic relevance...high value products 8th Edition of the Les Rendez-vous de Concarneau • September, 29 th and 30 th 2016" Where Industry