HIGH-THROUGHPUT SCREENING - Fisher Scientific · precision, speed, accuracy high-throughput screening insert document.qxd 6/4/02 12:42 pm page 1

P R E C I S I O N , S P E E D , A C C U R A C YH I G H - T H R O U G H P U T S C R E E N I N G

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Design Features Benefits to the User

• Stabilized laser • Avoid data biasingintensity

• Individual PMT • Maximize sensitivitydetectors for each • Maximize scanning speedlaser channel

• Stable scanning • Accurately measure down tomechanism 3mm pixel resolution

• Data channels perfectly aligned;no need for software correction

• Optimized confocal • Maximum light collection optics efficiency

• Discards stray light and background noise

Table 1. ChipReader Features and Benefits.

Virtek ChipReader Microarray ScannerIntroductionA microarray scanner reads the fluorescentsignal of each spot arrayed on a slide. But not all microarrays are alike. Scientistsneed an instrument that can deliver high sen-sitivity, ease of use, and broad compatibilityacross a wide range of slide sizes and types.The Virtek ChipReader Microarray Scannerfeatures exceptional sensitivity, flexibility andspeed, and delivers wide, linear dynamicrange for applications including genomics,proteomics, gene expression analysis, SNPanalysis, diagnostics, pharmaceutical R&D,and microarray QC.

SensitivityGene expression analysis can involve measur-ing small differences in gene expression lev-els. Furthermore, according to Murphy's law,many important signals are the least intense,increasing the difficulty of observing a signifi-cant change in gene expression. The scannermust therefore be able to distinguish thesefaint signals from the background noise. The Virtek ChipReader Microarray Scanner is designed with sensitivity as a key feature, and every aspect of the instrument has beenoptimized.

Improving Image QualityExcellent signal-to-noise ratios are not suffi-cient to create a superior quality microarrayimage. An image that looks good to the eyemay possess poor dynamic range, which usually results in a lack of gene-expression-level discrimination ability, which can turn aseemingly good image into one that containslittle useful information. The reason: types ofglass on which most microarrays are madehave different background fluorescence levels.And to date, there is no single protocol formaking and using microarrays, which exacer-bates the problem. Therefore, the scannershould have the ability to vary the laser anddetector settings to accommodate different slide types.

Table 2. Common Causes of Poor Dynamic Range

SummaryThe Virtek ChipReader Microarray Scannerprovides the user with exceptional sensitivity,flexibility and speed, and delivers a wide, linear dynamic range. The dynamic range can be increased through the unique ability of the ChipReader to control laser intensity and detector sensitivity accurately.

The Virtek ChipReader Microarray Scannerhas built-in dynamic range maximization toolsthat are easy to use and help you generatebetter-quality data.

Virtek Vision Corp. is a precision laser prod-ucts company with over 16 years’ experiencein laser imaging, projection, measurementand manufacturing, over five major businessdivisions. Our expertise in controlling lasersresults in the finest microarray scanner on the market today.

The Virtek ChipReader Microarray Scannerhas built-in dynamic range maximization toolsthat are easy to use. The software interfaceallows the user to see the improvements indynamic range in real time while the laserand detector settings are changed.

How to Improve Data Quality with the

It may come as a surprise that using full laserpower and maximum detector sensitivity cancreate a poor image; but this approach usual-ly reduces the dynamic range, and thus theability to discriminate between small changesin DNA expression levels. Often, the bestdynamic range is attained by using low laserpower and medium detector sensitivity set-tings. To do this, the scanner must have veryaccurate and fine laser and PMT control.

The Virtek ChipReader Microarray Scannercan increase the dynamic range through itsunique ability to control the laser intensity andthe detector sensitivity with accuracy.

The scanner’s settings can be adjusted from0 to 100% in increments of 1% or less. Otherscanners may give you a limited range ofchoices, usually 0%, 30%, and 100%, withnothing in between.

tech notesFrom Fisher Scientific Biotrack Summer 2001

MICROARRAY

Cause Effect

• Laser Intensity set • Signal Saturation

too high • Flattens high end of achievable

dynamic range

• Magnifies noise and reduces

signal-to-noise ratios

• Detector set at • Detector is “blinded” by exces-

maximum sive signal and increased back-

sensitivity ground noise levels

• Limits low end of achievable

dynamic range

Figure 1.The image onthe left wasscanned at relatively highlaser anddetector set-tings andexhibits poordynamicrange. Noticethe relativelypoor signal-to-noise ratiosand the satura-tion exhibitedby spots 1–3.In contrast,the image onthe right was

scanned at 50% laser intensity and reduceddetector sensitivity. The dynamic range hasimproved, saturation has been eliminated,and the signal-to-noise ratios for spots 1–3have increased. Whichever scan the userprefers, the ChipReader offers the tools toget the best image.


MICROARRAY

FAX: 1-800-926-1166 ONLINE: fishersci.com PHONE: 1-800-766-7000

Description Catalog No. PriceScanners (Resolution Range)Regular (10–80µm, 10µm Increments) B013-VIR6801 $52,800.00High (5–40µm, 5µm Increments) B013-VIR7001 $59,800.00Extreme (3–24µm, 3µm Increments) B013-VIR6901 $69,800.00Accessories and UpgradesBarcode Reader B013-VIR29700 $500.00Flat-Panel Display Upgrade † B013-VIR30700 $1,400.00

† When ordered as an upgrade, the flat-panel monitor with 15� (38cm) display areais included with the ChipReader system instead of the standard 17� (43cm) CRT monitor.

SpecificationsChip Size Standard 1x3� (25x76mm) microslideScan Area Up to 22x65mmRead Time 20x20mm in less than 1 min. at 20µm pixel

size; less than 2 min. at 10µm pixel size.Read Sequence All dyes, simultaneously or sequentiallyResolution Eight software-selectable options from

base of 3, 5 or 10µm, depending on systemDynamic Range 16 bits/pixel; linear detection over entire

rangeData Storage 16-bit TIFFLasers Two (standard) solid-state semiconductor;

with standard excitation wavelengths of 532nm and 635nm and power up to 10mW for each laser

Control Computer Windows NT-based PC compatible, Pentium processor, 1,400MHz, 1GB RAM,17� color CRT monitor, 40GB Hard Disk,CD/RW Drive

Software Functions Microarray grid generation, data collection, image display and storage, data evaluation and storage, look-up table editor, and com-plete user control of all system functions,SNR and DR tools.

LxWxH 11x9x12� (28x23x30cm)Power Requirements 90–260V 50/60Hz, 150W

Step out of the background—with the

Virtek ChipReader Microarray Scanner• Exceptional sensitivity

• Laser confocal system

• Unmatched throughput

• Smallest footprint

• Rapid, simultaneous dye scanning

Super-sensitive scanningDesigned with superior light-collection efficiency, ChipReader uses low laser power—and lets you control the lasers for each channel independently. Results: lower noise floor, improved weak-signal detection, longer dye life and virtually no photobleaching of slides.

Greater scanning speedScan time is reduced by using both red and green lasers to simultaneously excite two different fluorescent dyes. User-selected scanning speed—5 to 30 lines per second—accommodates individual experiments and sample evaluations.Reads 20x20mm area in less than one minute. Scanned imagesregister perfectly, with no image registration step required.

Broad slide compatibilityModular slide holder offers the flexibility to use a variety of slide sizes, with a scan area of up to 22x65mm. Scannerreads glass (top or bottom), nylon, plastic, and silicon sub-strates—slides can be read wet or dry, with or without a cover slip.

Flexible and easy to useChipReader lets you adjust and optimize your scanning parameters in real time, by controlling the laser power output, multiple detector sensitivity and focus. The system softwareallows you to set and save experimental performance parame-ters, to make changes quickly—without disturbing your sample.

Reliable support and serviceChipReader systems are supplied with control computer, software, one-year warranty (from invoice date) on all parts and labor, and free software upgrades throughout the warranty period.

Fisher Ordering Information


MICROARRAY

IntelliGene DNA Microarray Series†

New!TaKaRa’s IntelliGene Series allows quick and simple, high-throughput analysis of gene expression. These arrays contain denatured, double-stranded cDNAs arrayed on standard 1x3� glassslides. All clones have been tested to ensure good signal and are designed for reduced background.

IntelliGene Microarrays are appropriate for dual-color analysis on a single microarray using high-resolution fluorescent detection.Available for analysis of human cancer, human cytokines, endocrinedisruption, cyanobacterial ORF, Arabidopsis and E. coli gene analysis.

† TaKaRa's IntelliGene DNA Microarrays are manufactured and sold under a licensing agreement with Affymetrix, Inc.

‡ Product version and number of genes spotted may be subject to upgrade. Please visit the TaKaRa Web site at http://bio.takara.co.jp/BIO_EN/ for the latest product information or to download a complete list of the genes spotted on each IntelliGene microarray.

BIOMEDICALS

Figure 2. Comparison of Synechocystis sp. gene expression under lightand dark conditions using CyanoCHIP. Analysis indicates upregulated genes(red spots) or downregulated genes (green spots) under dark conditions.

Figure 1B. Gene expression after 6 hours in dark conditions.

from TaKaRa & PanVera

Scatter plot analysis of Synechocystis sp.gene expression using CyanoCHIP

Figure 1A. Gene expression under light conditions.

IntelliGene Number of Number Catalog PriceDescription Genes of No.

Spotted‡ SlidesHuman Cancer 5550 (V.3.0) 2 B013-TAK X102 $550.00CHIP

Human Cytokine 5220 (V.2.0) 2 B013-TAK X104 $350.00CHIP

DNA CHIP, 5220 (V.1.0) 2 B013-TAK X103 $350.00for EndocrineDisruption Study

CyanoCHIP 52,800 (V.1.2) 1 B013-TAK X001 $850.00E. coli CHIP 54,000 (V.1.0) 1 B013-TAK X003 $900.00Human 1K Set I 51,000 (V.1.0) 2 B013-TAK X1071 $850.00Arabidopsis 52,200 (V.1.0) 1 B013-TAK X002 $600.00CHIP I

TestARRAY 90 (V.3.0) 2 B013-TAK X000 $200.00Mouse CHIP Set I 5900 (V.1.0) 2 B013-TAK X2011 $850.00Human Apoptosis 5201 (V.1.1) 2 B013-TAK X101 $300.00CHIP



MICROARRAY


Bar Code Packaging Catalog No. Case of 25Yes In pouches of 5 B013-07-200-008 $312.00Yes Bulk B013-07-200-006 $300.00No In pouches of 5 B013-07-200-009 $297.00No Bulk B013-07-200-007 $285.00

Corning CMT-GAPS II Slides:ultraflat glass enhances accuracy & performance!Same uniform spot morphology, high signalstrength, and ultralow backgroundNew CMT-GAPS II coated slides provide the same innovative coatingprocess and attachment chemistry as the CMT-GAPS amino-silaneslides you’ve come to depend on, and can even be used with thesame protocols.

Now with even less variation in thickness!25x75mm CMT-GAPS II slides incorporate a new Corning propri-etary ultraflat glass that ensures more accurate reading by confocallaser scanners—and enhanced microarray performance.

Smaller bar codes for added convenienceDurable, ceramic-fused bar codes traceable to manufacturing lots arenow smaller to allow increased printing area. For printing over theentire surface, slides without bar codes are also available.

Zylux Luminometers

Sirius Luminometer Catalog No. PriceWith printer B013-12-200-17 $5,650.00With printer and one injector B013-12-200-18 $7,200.00With printer and two injectors B013-12-200-19 $8,170.00

Orion Micro Luminometer Catalog No. PriceWith one injector B013-12-200-15 $19,550.00With two injectors B013-12-200-16 $21,500.00

Sirius Single-TubeLuminometer

• High sensitivity

• Flash and glow

applications

• Single and dual

measurement mode

• Internal printer for

dataThis universal instrument for single samples has a state-of-the-art photon counter with a spectral range of 370–630nm. The measuringchamber has a retractable drawer with interchangeable reflectorsand sample adapters. Features sensitivity better than 1,000 moleculesof Luciferase in a reporter gene assay. ATP detection limits set only by reagent quality. Basic software is included.

Orion Microplate Luminometer

• High sensitivity

• Over 6 decades of

linear range

• Available with 0 to

3 injectors

• Optional tempera-

ture control

• DLReady—qualified by PromegaThe Orion is a modular, ultrasensitive, easy-to-use photon-countingluminometer. This compact unit incorporates all functions necessaryfor measurements of glow-type luminescence. Add one or more injection units, and you can perform virtually anyflash-type luminescence measurements. Windows-based Simplicity PC software provides completeinstrument control and data analysis.

Top Performers—and Easy to Use!





READERS/WASHERS/LIQUID HANDLING

Wellwash AC Microplate Washer

• Residual volumes <3µL

• Robotics compatible!

• Secure wash changes with

automated liquid purgingMultibuffer, two-strip wash head system isfast, reliable and needs minimal setup time.Fully integrated orbital shaker provides thorough washing, reduced soaking times.Flexible programming lets you store up to 99wash programs with up to 4 linked protocolsper program.

Model (Volume Range) Packaging Catalog No. Price10 (0.2–10µL) 10x96/rack B013-21-377-208 $54.0020 (0.2–20µL) 10x384/rack B013-21-377-802 $289.00250 Universal (5-250µL) 1,000/bag B013-21-377-45 $35.00250 Universal (5-250µL) 96/rack B013-21-377-2 $47.00300 (5-300µL) 1,000/bag B013-21-377-49 $39.00300 (5-300µL) 10x96/rack B013-21-377-128 $52.001000 (100–1,000µL) 10x60 rack B013-21-377-189 $32.00Filter Tip 10µL Micro 10x96/rack B013-21-377-354 $129.00Filter Tip 50µL 10x384/rack B013-21-377-355 $75.00Filter Tip 100µL 10x96/rack B013-21-377-170 $99.00Filter Tip 200µL 10x96/rack B013-21-377-171 $99.00Filter Tip 300µL 10x96/rack B013-21-377-307 $99.005mL (1–5mL) 75/box B013-21-377-50 $12.005mL (1–5mL) 500/bag B013-21-377-304 $65.005mL (1–5mL) 5x24/rack B013-21-377-165 $25.0010mL (2–10mL) 40/box B013-21-377-146 $7.0010mL (2–10mL) 100/bag B013-21-377-52 $16.0010mL (2–10mL) 5x24/rack B013-21-377-167 $30.00

Finnpipette Digital Single-Chan-nel and Multichannel PipettersSuper blowout performance for microvolumeprecision in a new, streamlined design.

• Soft-touch tip ejection

• Recalibrate in the lab

• Fully autoclavable

• 11 single-channel and 7 multi-

channel models to choose from

• Accept both Finntips and generic

brand tips

• Three-year warranty

Description Catalog No. PriceWellwash AC B013-14-386-76 $6,850.00

Description Tip Sizes Catalog No. PriceSingle Channel0.2–2µL 10, 20, 50 B013-21-377-190 $249.000.5–10µL, Micro 10, 20, 50 B013-21-377-191 $249.000.5–10µL 250 Univ. B013-21-377-192 $249.002–20µL 250 Univ. B013-21-377-287 $249.005–40µL 250 Univ., 300, 200 Extended B013-21-377-193 $249.0010–100µL 250 Univ., 300, 200 Extended B013-21-377-288 $249.002–200µL 250 Univ., 300, 200 Extended B013-21-377-289 $249.00200–1,000µL 1,000, 1,000 Extended B013-21-377-195 $249.00100–1,000µL 1,000, 1,000 Extended B013-21-377-290 $249.001–5mL 5mL B013-21-377-196 $249.002–10mL 10mL B013-21-377-197 $259.008-Channel0.5–10µL 10, 20, 50 B013-21-377-198 $569.005–50µL 250 Univ., 300, 200 Extended B013-21-377-199 $569.0050–300µL 250 Univ., 300, 200 Extended B013-21-377-200 $569.0012-Channel0.5–10µL 10, 20, 50 B013-21-377-365 $699.005–50µL 250 Univ., 300, 200 Extended B013-21-377-201 $699.0050–300µL 250 Univ., 300, 200 Extended B013-21-377-202 $699.0016-Channel5–50µL 50 B013-21-377-807 $749.00

Finntips

• Chemical resistant

• High-quality

polypropylene

• AutoclavableKnown for quality throughoutthe world, high-quality Finntipswork with Finnpipette modelsand with most other pipetbrands!

high-tech, precision liquid handling!

From Thermo Labsystems:




ThermoLabsystems




Volume Packaging Catalog No. Pack ofFor Beckman Multimek 96Natural Polypropylene/Genomic Nonfilter50µL 96 tip rack B013-02-707-210 960/$39.9550µL 96 tip rack, sterile B013-02-707-211 960/$41.95Blue Polypropylene/Genomic Nonfilter250µL 96 tip rack B013-02-707-216 960/$39.95250µL 96 tip rack, sterile B013-02-707-217 960/$41.95Blue Polypropylene/Genomic Aerosol-Barrier Filter250µL 96 tip rack B013-02-707-212 960/$79.95250µL 96 tip rack, sterile B013-02-707-213 960/$81.95For Beckman Biomek 1000/2000Natural Polypropylene/Genomic Nonfilter1–20µL Nonsterile Bulk Bag B013-02-707-235 1,000/$25.951–20µL 96 tip rack B013-02-707-236 960/$37.951–20µL 96 tip rack, sterile B013-02-707-237 960/$41.955–250µL Nonsterile Bulk Bag B013-02-707-238 1,000/$25.955–250µL 96 tip rack B013-02-707-239 960/$37.955–250µL 96 tip rack, sterile B013-02-707-240 960/$41.95Natural Polypropylene/Genomic Aerosol-Barrier Filter20µL 96 tip rack, sterile B013-02-707-241 960/$89.95180µL 96 tip rack, sterile B013-02-707-242 960/$89.95

Fisherbrand Lab Automation Pipet TipsMaximum performance, accuracy, reproducibility!For the most demanding HTS and laboratoryautomation proceduresEnhancing an already comprehensive Fisherbrand tip line, these new lab automation pipet tips have been designed specifically forcompatibility, accuracy, and reproducibility.

Engineered to fit the most popular robotic systems used today!

• Beckman Biomek 1000/2000

• Beckman Multimek 96

• Zymark Rapid Plate 96/384

• Qiagen BioRobot 9600, 9604, and Rosys

• Packard MultiPROBE I

• Packard MultiPROBE II

• Tecan GENESIS and Cavro

• Molecular Devices FLIPR 2

Specially designed racks with individual pipet tip wells Effectively reduce the risk of cross-contamination and provide optimalfit, function, and workstation productivity.


Choose nonfilter or aerosol-barrier filter tipsIn a wide assortment of volume sizes and styles.

Available in clear, blue-tint, or carbon black conductive polypropyleneCarbon black, liquid-level-sensing tips are now required by manyautomated systems. They are constructed of a highly conductive material that is necessary to detect the presence or absence of sufficient liquid, in order to avoid short sampling and excessive submersion of the tip during aspiration.

(Many additional Fisherbrand Lab Automation Tips are availablebesides those listed here! For ordering information, contact yourFisher Representative, or check it out at www.fishersci.com.)

Tips for Beckman Biomek 1000 and 2000

Fisherbrand



† The Polymerase Chain Reaction (PCR) process is covered by U.S. Patents 4,683,202; 4,683,195; and 4,965,188 or their foreign counterparts owned by Roche Molecular Systems, Inc. and F. Hoffman-La Roche Ltd. No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of these various products.

Description Catalog No. PriceFLx800 Fluorescence Readers: all come with 2 excitation and 2 emission filtersWith bottom 5mm probe only B013-BT-FLX800B $11,500.00With top 3mm probe only B013-BT-FLX800T $11,500.00With top/bottom (3/5mm) probes B013-BT-FLX800TB $13,000.00With top/bottom probes, extended range PMT B013-BT-FLX800TBE $13,500.00With top/bottom probes, extended range; reads 6- to 384-well plates and PCR tubes B013-BT-FLX800TBP $14,000.00µQuant Universal Microplate Spectrophotometer B013-BTMQX200 $11,500.00KC4 v3.0 SoftwareCD-ROM B013-BT5290500 $1,875.00Network Package: network key, operating manual B013-BT5260505 $500.00KCjunior SoftwareCD-ROM B013-BT5270501 $975.00LabRack B013-BT48397 $390.00

µQuant Universal MicroplateSpectrophotometer

• Continuous wavelength selection;200–999nm range

• Reads 6-, 12-, 24-, 48-, 96- &384-well plates plus Terasakitrays

• Automatic pathlength correction• 1cm measurements with optional

Bio-Cell• Over 25 preprogrammed assays• Automated nucleic acid

quantitation

FLx800 Fluorescence Readers• Read multiwell plates, Terasaki

trays & PCR† tubes • Select models read both fluores-

cence & luminescence• Choose onboard data analysis;

or, use with KCjunior software onyour own PC!

• Feature automated top/bottomprobe switching

• Complete with onboard diagnostic testing

KC4 v3.0 Software

• Exclusive PowerReports customreporting tool

• Endpoint, kinetic, multiwave-length & spectral scanning

• Data collection from 6- to 384-well plates

• Well area scanning• Expansive kinetic analysis,

including real time kinetic display

• Multi-user password access• Data & protocol audit trails

KCjunior Software• Endpoint, kinetic, multiwave-

length & spectral scanning• Data collection from 6- to

384-well plates• Multiple curve fit choices• Intuitive protocol creation• Automatic export

LabRack• Sturdy, compact design• Can hold readers, washers,

laptop PC, or monitor• Adjustable shelves for flexibility

Synergize! Automated Instrumentation andMicroplate spectrophotometers, fluorescence readers & software!


KC4 Software

Lab Rack with: optionallaptop & KCjuniorSoftware; µQuantSpectrophotometer; FLx800 FluorescenceReader




Precision 2000 AutomatedPipetting System

• Configurable six-station platform &unique X/Y transport for effortless96- to 384-well plate transfers

• Efficiently executes routine liquiddeliveries—reagent addition, platereplication & serial dilutions

• Proprietary pipet technology provides reliable tip pickup

• Open design guarantees compatibility with robotic plate handlers

• Optional rapid 8-channel manifold simplifies continuousreagent dispensing

• Precision Power software forWindows: simple, complete program creation & control

PowerWaveX Select MicroplateSpectrophotometers

• Fast 96- & 384-well reading• Continuous wavelength selection

from 200 to 999nm• 4-Zone temperature control to 50°C

Description Catalog No. PricePrecision 2000Automated Pipetting System B013-BT-PRC384 $14,000.00Automated Pipetting System B013-BT-PRC384M $16,000.00

with eight-channel manifoldPrecision Power PC Software B013-BT-PRCPWR $1,075.00Pipet Tips (nonsterile)

50µL; 96/rack B013-BT-98183 960/$43.50250µL; 96/rack B013-BT-7112099 960/$43.50

ELx405 Microplate Washer ‡96-well only Automated Washer B013-BT-ELX405 $6,000.0096-/384-well Select Automated Washer B013-BT-ELX405U $11,500.0096-/384-well Magnetic Bead Washer B013-BT-ELX405M $10,000.00PowerWaveX Select Models with KC4 SoftwareMicroplate Spectrophotometer B013-BTPRWSE $20,000.00Microplate Spectrophotometer with 4-Zone temp. control B013-BTPRWSIE $21,000.00Microplate Spectrophotometer with robotic interface B013-BTPRWSRE $21,500.00Microplate Spectrophotometer with 4-Zone temperature B013-BTPRWSIRE $22,500.00

control and robotic interfaceFastrack TD Robotic Microplate HandlerWith tip/deep-well plate delivering option B013-BTFASTRACKT $18,000.00

Software from Bio-Tek Instruments!Automated pipetting, microplate handling and spectrophotometry


• Optional 1cm measurementswith patented Bio-Cell

• Endpoint, kinetic, & spectralscanning read modes

• Powerful KC4 software included• Automated pathlength correction• Robot compatibilty

Fastrack AutomatedMicroplate Handler

• Automates routine procedures• Links multiple instruments to

create a complete system• 345° rotation axis for maximum

capacity & rotational gripper forultimate configuration flexibility

• Works with standard & deepplates & tip boxes

ELx405 Select 96-/384-WellMicroplate Washer for HTS

• Integrates into robotic systems

• Patented Dual-Action manifold:

precise aspiration & dispensing

• Multiple customizable programs

• 96-well & magnetic bead models

available, too

Precision 2000 Automated Pipetting System

PowerWaveX Select Spectrophotometers

Fastrack Automated Microplate Handler

ELx405 Select Microplate Washer

‡ Some required accessories not included. Contact your Fisher Sales Representative for details.


PLATES

Streptavidin and Poly-D-Lysine-CoatedMultiwell Plates

Corning’s 96- and 384-well polystyrene plates are prepared with Streptavidin purified protein or synthetic Poly-D-Lysine, to give you high coating homogeneity!Streptavidin-Coated Plates are highly resistant to common detergents and featurelow leaching; Poly-D-Lysine-Coated plates foster increased survival in serum-free andlow-serum conditions for certain cell types and exhibit a uniform positive net charge that enhances cell attachment, growth and differentiation.

Streptavidin-CoatedMicroplates

• High binding capacity of biotin

• Superior temperature stability

• Preblocked for immediate use

Ideal for binding assay applications, includ-ing: immunoassay, kinase assay, virus detec-tion, immunoPCR and DNA hybridizationassay. Plates’ specificity improves signal-to-noise ratio and they exhibit typical bindingcapacity of )25 pmoles of d-biotin per well.

Plates have flat-bottom wells, and areavailable clear or opaque for minimal well-to-well crosstalk, low background.Supplied without lids. Storage at 2° to 30°Cfor up to 2 years. For research only.

Poly-D-Lysine-Coated Multiwells

• Superior cell binding

• Lot tested for cell attachment

performance, endotoxins,

bacteria and fungi

For culturing primary neurons, glial cells, neuroblastomas and transfected cell lines; suitable for applications including high-throughput cell-based assays and immunofluo-rescence/chemiluminescence microscopy.

Plates have optically clear bottoms, andare available with clear or opaque walls forminimal well-to-well crosstalk and low back-ground of fluorescence and/or luminescence.Supplied with clear polystyrene lids. Storage at 4° to 30°C for up to 2 years. For research only.

With Corning’s proprietary coating technology: stable, uniformsurfaces for reproducible results!

96-Well Multiwell Plates 384-Well Multiwell PlatesColor Catalog No. Case of 100 Catalog No. Case of 100Streptavidin CoatedClear B013-07-200-766 $1,610.00 B013-07-201-146 $2,267.00Black B013-07-200-767 $1,687.00 B013-07-201-147 $2,557.00White B013-07-200-768 $1,687.00 B013-07-201-148 $2,557.00Poly-D-Lysine CoatedClear B013-07-201-136 $680.00 B013-07-201-133 $1,200.00White/Clear Bottom B013-07-201-137 $1,198.00 B013-07-201-134 $1,786.00Black/Clear Bottom B013-07-201-138 $1,198.00 B013-07-201-135 $1,786.00



PLATES & TUBES


Other sizes are available. Contact yourFisher Sales Representative for details.

Description Color Volume Catalog No. Pack ofNonsterile; Bulk Packed Natural 1.5mL B013-02-681-320 250/$18.90

Red 1.5mL B013-02-681-312 250/$18.90Blue 1.5mL B013-02-681-313 250/$18.90Green 1.5mL B013-02-681-314 250/$18.90Yellow 1.5mL B013-02-681-315 250/$18.90Orange 1.5mL B013-02-681-316 250/$18.90Violet 1.5mL B013-02-681-317 250/$18.90Amber 1.5mL B013-02-681-318 250/$18.90Mixed 1.5mL B013-02-681-319 250/$18.90

Sterile; Individually Wrapped Natural 1.5mL B013-02-681-331 250/$61.95Certified; Bulk Packed Natural 1.5mL B013-02-681-461 250/$21.50

Fisherbrand Microcentrifuge Tubes with Snap Caps

Fisherbrand

Fisherbrand Well Plates• 96 or 384 wells

• Round bottoms, 4 different colorsPolypropylene plates resist many chemicalsand offer minimum binding. Come in fourcolors—ideal for storage!96-well plates have generous 0.5mL totalwell volume, withstand temperatures from–80° to +121°C, and are optimized for HTS.384-well plates let you process 4x the sam-ples in the same 86x128mm footprint, withworking volumes of 10 to 100µL. 384-Well Plates

Total well volume is 120µL; withstand temperatures from –80° to +80°C. Higher well density, too, for economicaluse of expensive reagents!

Both well plate styles are compatiblewith robotics, automated readers and liquid-handling systems.

All plates are supplied nonsterile, without lids. (Clear polystyrene lids, Cat. No. B013-12-565-511, are available in cases of 180 for $166.83.)

96-Well Plates

• Withstand 17,000–30,000xG

(RCF)

• Resist autoclaving to 120°C

and freezing to –80°CThese durable, chemical-resistant polypro-pylene siliconized/low retention flat topmicrocentrifuge tubes come with snap capsfor a secure seal—even over extended periods. They’re specially designed for usein critical applications where minute samplerecovery counts. Every tube has graduationmarks for quick volume estimating and frosted writing area for easy ID. They’reautoclavable up to 120° and withstand temperatures as low as -80°C.Certified tubes (natural color) are tested and certified by an independent laboratoryto be RNase- and DNase-free. Supplied inbulk packs.Nonsterile tubes are available in eight colors and are supplied in bulk packs. Sterile tubes (natural color) come individ-ually wrapped.


96-Well Plates 384-Well PlatesPlate Total Catalog No. Case of Working Catalog No. Case ofColor Volume VolumeNatural 0.5mL B013-12-565-502 80/$143.48 10–100µL B013-12-565-507 120/$434.70Red 0.5mL B013-12-565-503 80/$143.48 10–100µL B013-12-565-508 120/$434.70Yellow 0.5mL B013-12-565-504 80/$143.48 10–100µL B013-12-565-509 120/$434.70Blue 0.5mL B013-12-565-505 80/$143.48 10–100µL B013-12-565-510 120/$434.70



PLATES

Quality NUNC HTS Labware!

NUNC 1536-Well Plates

NUNC Optical-Bottom Plates

Fisher Ordering Information: NUNC 384-Well Optical-Bottom Plates

Fisher Ordering Information: NUNC 96-Well Optical-Bottom Plates

Optimum clarity for viewing well contents• Bases of either #1.5 German coverglass or crystal

clear polystyrene • Clear, black, or white polystyrene upper structures• Compatible with robotics and automated

liquid-handling systems• 96-well plates feature flat-bottom well geometry;

384-well plates have rounded square wells

Choice of three assay surfaces• Cell culture for cell-based assays • Non-Treated for liquid phase and bead assays• MaxiSorp for sandwich ELISA, protein quantitation

assays, ligand receptor interaction and other molecule-based assays

Color Lid Sterile Catalog No. Case ofClear Yes Yes B013-12-565-455 60/$1,122.00

No No B013-12-565-459 10/$160.00No No B013-12-565-453 90/$1,350.00

Black Yes Yes B013-12-565-454 60/$1,056.00No No B013-12-565-458 10/$170.00No No B013-12-565-451 90/$1,350.00

White Yes Yes B013-12-565-456 60/$990.00No No B013-12-565-457 10/$180.00No No B013-12-565-452 90/$1,530.00

Color Lid Sterile Catalog No. Case of 30Non-Treated Surface, Coverglass BaseBlack No No B013-12-566-38 $360.00Non-Treated Surface, Polymer BaseBlack No No B013-12-566-72 $129.00White No No B013-12-566-73 $132.00Cell Culture-Treated, Coverglass BaseClear Yes Yes B013-12-566-36 $450.00Black Yes Yes B013-12-566-35 $450.00White Yes Yes B013-12-566-37 $450.00Cell Culture-Treated, Polymer BaseBlack Yes Yes B013-12-566-70 $144.00Black No Yes B013-12-566-77 $133.50White Yes Yes B013-12-566-71 $147.00White No Yes B013-12-566-76 $136.50MaxiSorp Surface, Polymer BaseBlack No No B013-12-566-74 $133.50White No No B013-12-566-75 $136.50

Color Lid Sterile Catalog No. Case of 30Non-Treated Surface, Coverglass BaseBlack No No B013-12-566-34 $510.00Non-Treated Surface, Polymer BaseBlack No No B013-12-566-2 $192.57White No No B013-12-565-355 $207.02Cell Culture-Treated, Coverglass BaseClear Yes Yes B013-12-566-31 $600.00Black Yes Yes B013-12-566-32 $600.00White Yes Yes B013-12-566-33 $600.00Cell Culture-Treated, Polymer BaseBlack Yes Yes B013-12-566 $210.06Black No Yes B013-12-565-473 $113.10White Yes Yes B013-12-566-1 $216.43White No Yes B013-12-565-850 $205.80MaxiSorp Surface, Polymer BaseBlack No No B013-12-566-5 $196.03White No No B013-12-566-6 $205.65

Exceptionally flat design allows reliable plate-mapping• Flatness varies only 0.25mm across the plate!• Highly compatible with automated systems for

ultra HTS• Features rounded square well with flat bottom• Skirted rim allows labeling and bar coding• Available non-treated in clear, black, or white

Fisher Ordering Information: NUNC 1536-Well Plates

NUNC ™

Brand Products


AUTOMATED DNA PURIFICATION


Description Quantity Catalog No. PriceMini-Prep 24 1 B013-MP2400 $8,412.00Mini-Prep 48 1 B013-MP4800 $9,999.00Sample Cassettes with Applicator Comb/Lysis Tablets 10 (120 samples) B013-C1201-AC $100.00Short Body Sample Cassettes with Applicator Comb/Lysis Tablets 10 (120 samples) B013-C1202-SB $110.00Fluorescent Sequencing Desalting Plate 1 (96 samples) B013-FSP-96 $19.00Replacement Applicator Combs/Lysis Tablets For 10 cassettes B013-ACT-10 $20.00100X Running Buffer (makes 25L) 250mL B013-C1500 $18.00High-Yield Bacterial Growth Media 300g B013-MR2001-3 $46.00

1kg B013-MR2001-10 $149.002kg B013-MR2001-20 $285.00

Extended 1-Year Warranty on Mini-Prep 24 or Mini-Prep 48 1 B013-WA100 $400.00

Automated Plasmid Mini-Preps fromMacConnell Research

For Maximum Purification and Performance:

• Produce sequencable-grade plasmid DNA in just over

1 hour—directly from liquid culture

• No centrifugation required

• Bacteria culture loads directly into cassette lanes

with microtiter spacing

• Size range: 2–15kb on standard programs

• Small footprint—only 14x20�

Fully automatic, microprocessor-controlled! Feature electrophoretic separation of DNA based on several user-selected programs. Use precast, disposable cassettes, which fitinternal electrophoresis processing rig; each holds up to twelve 2.5mLsamples. Mini-Prep 24 holds 2 cassettes; Mini-Prep 48 holds 4.

Produce high-purity plasmid DNA—suitable for use in most molec-ular biology processes, including manual and fluorescent automatedDNA sequencing. With high-copy-number plasmids, individual cas-sette sample lanes typically yield 4–6µg of DNA from 2mL of culture.

Disposables required include: sample cassette, running buffer,applicator comb, lysis tablets, bacterial growth media and fluorescentDNA sequencing desalting plate.

Step 1: Lysis. Bacteria are lysed directly in media with enzymes andreagents that degrade RNA and proteins and dissolve bacterial membranes.Reagents supplied with each cassette.

Step 2: Resolution. Automatically fills with running buffer, then separates plasmid DNA from digested cellular components. Plasmid DNA from 2 to20kb is electrophoresed until the 2kb supercoiled plasmids run to the endof (but not off) the separation gel.

Step 3: Electroelution. A microchamber block, automatically slid in placeby rotating cams, accomplishes electroelution and concentration of purifiedplasmid. High cutoff filtration membrane in block collects purified DNA asa film.

Step 4: Draining & Recovery. After electroelution, Mini-Prep automatically drains running buffer and collects purified DNA in a 25µL droplet withinthe microchamber block window. Remove finished DNA with a disposable-tip pipetter or multichannel pipetter with microtiter plate spacing.

B013-MP4800

How It Works:



High-Throughput ScreeningElectroporation involves the application ofcontrolled pulses of electrical fields to bio-logical systems. The electrical fields inducetransient pores in the cell membrane, allowing entry of molecules of various kinds.Electroporation has been widely used to transform mammalian, bacterial, yeast, fungal, and plant cells.

Conventional electroporation is performedin a cuvet, where the sample is enclosed bytwo parallel aluminum plates that generate ahomogeneous electrical field.

High-throughput screening and cDNAlibrary construction require the handling ofmultiple samples simultaneously. Manyresearchers use BTX Electroporation Cuvettesin a high-throughput setting; however, this use of conventional cuvets is costly and timerestrictive. For these types of applications, performing electroporation directly in a 96-well plate is ideal. BTX Instruments has 8-well and 96-well coaxial electrodes for this application.

• Researchers at UCLA used the 8-well electrode to deliver DNA to HEK 293 cells with 20% transfection efficiency.1

• Researchers at NIH used the 96-well electrode to deliver plasmid DNA to Jurkat cells with 18% efficiency and 90% viability.2

• Hoffman-Tsay et al 3 used the 96-well coaxial electrode to test eight chemicals in plant fusion experiments.

• Peterfy et al 4 successfully delivered DNA into COS 7 cells using multiwell electrodes,

and they found this method easy and rapid to perform. Labor and supply costs were reduced because cuvets were not required. The amount of plasmid DNA required was also lower when electropor-ation was performed in a 96-well plate.

• Marrero et al 5 used the 8-well electrode to electroporate antibodies into rat vascular smooth muscle cells to induce cell prolifer-ation. Their studies helped identify two sig-naling pathways that could be targets for future therapeutic interventions in cardio-vascular disease.

Expanding Roles for Electroporation:

ECM 630 Exponential Decay Wave Generator ECM 830 Square Wave Generator

HTS, large-volume processing, and ex vivo gene therapy

tech notesFrom Fisher Scientific Biotrack Summer 2001

HIGH-THROUGHPUT CLONING

A Division of Genetronics, Inc.

8- and 96-well coaxial electrodes

References1. Personal communication with Graeme Dougherty,

UCLA.

2. Personal communication with Jim Smith, NIH/NCI.

3. Hoffmann-Tsay et al, Bioelectrochemistry and Bioenergetics, v. 34, pp. 115–122, 1994. (BTX ID #3846)

4. Peterfy, Miklos et al, Methods in Molecular and Cellular Biology, v. 5(6), pp. 353–362, 1995, USA. (BTX ID #3545)

5. Marrero, Mario B. et al, Journal of Biological Chemistry, v. 272 (39), pp. 24684–24690, 1997, USA. (BTX ID #3008)

6. Personal communication with Ken Beckman, Division of Biochemistry and Molecular Biology, University of California, Berkeley. (BTX Protocol #285)

7. Personal communication with Dr. Maria Ragland, Research Genetics, Inc., Huntsville, Alabama. (BTX Protocol #338)

8. Parham, Janet H. et al, Cytotechnology, v. 28, pp. 1–9, 1999, USA. (BTX ID #3995)

Use of BTX ECM 830 Square WaveGenerator with the 8- or 96-well coaxial electrodes is ideal for high-throughput mammalian or plant cell electroporation. For transformation of E. coli, BTX offers theECM 630 Exponential Decay Wave Generatorwith the 8-well coaxial electrode or cuvets. Theaverage transformation efficiencies range from106 transformants/µg DNA (coaxialelectrode)6 to 1010 transformants/µg DNA(cuvets).7

Large-Volume Processing (LVP)and Ex Vivo Gene Therapy Large-volume sample processing for industrial-level production or ex vivo gene therapy isalso in demand. Experiments may be scaledup by first optimizing conditions in cuvets andthen transferring the protocol to a flow-through setup.

BTX Electro Flow Porator Systems consist of a pump and a modified electroporator.

The pump can be programmed and operated in sync with the electroporator to deliver pulses to batches of cells.

• Parham et al 8 optimized transient gene expression in CHO, COS 7, HEK 293, NSO, and CV-1 cells using the flowthrough system. Their data indicate that flow electro-poration provides an efficient method of DNAdelivery into large numbers of cells for mam-malian protein production.


HIGH-THROUGHPUT CLONING


Description Catalog No. Price8-Well Electrode B013-BTX747 $1,875.0096-Well Electrode B013-BTX840 $5,995.00Single-Well Electrode B013-BTX4911 $275.00

ECM 830 Square Wave Electroporator

• Transforms bacteria, yeast; transfects mammalian cellsFor in vitro, in vivo and in ovo electroporation. Delivers gentle squarepulses to yield high viability in mammalian cell transfection. Achieve primary cell electroporation using BTX in situ electrodes (Petri dish,Petri-Pulser, Coaxial and Epizap electrodes) with high efficiency andreproducibility. Wide range of voltage and pulse length settings: use itin nuclear transfer techniques, gene therapy electroporation, in ovoand in vitro embryo gene delivery, plant protoplast transformation.

Comes with 30 sterile, disposable BTX Cuvettes (1mm, 2mm and4mm gap sizes), Cuvette Safety Stand, and 20-position numberedCuvette Rack.

Gap Size Volume Catalog No. Pack of 501mm; with micropipet 70µL B013-BTX610 $130.002mm; with micropipet 400µL B013-BTX620 $130.004mm; with micropipet 800µL B013-BTX640 $130.00

ECM 6000F Electro Flow Porator System

• Transforms/transfects large volumes of cells,

including prokaryotes and eukaryotesPerfect choice for ex vivo applications! Offers over 1,000 pulselengths, two voltage ranges, ten timing resistors, dual-display monitor-ing. Switches effortlessly between bacterial transformation and plant ormammalian transfection. Program system’s peristaltic pump with flow-rate and pulse time based on your parameters. Short-circuit-proof,arcproof design.

Includes a SciLog pump with I/O port, exponential decay genera-tor, safety stand, 5 sterile gold-plated flowthrough chambers (3.2mmor 2mm gap), and tubing.

BTX Electroporators, Electrodes and CuvettesTransforming Science:

A Division of Genetronics, Inc.

Coaxial ElectrodesFor electroporation of adherent or suspension cells grown in 96-wellplates. Single-Coaxial and Multi-Coaxial (8-well and 96-well) models;all are circular, and produce divergent fields for electroporation of upto 0.3mL. Easy soap and water cleanup; sterilize with alcohol. Withdrying chamber.

Description Catalog No. PriceECM 6000F B013-BTX6000F $11,605.00


Description Catalog No. PriceECM 830 B013-BTX8300 $6,485.00




Electroporation Cuvettes Plus

• For transfection & transformation

of bacteria, yeast, insect, plant

and mammalian cellsCompatible with most electroporation systems. In 1, 2, and 4mm gap sizes; embedded aluminum electrodes have round, color-coded caps. Each pack includes sterile micropipet for sample removal. Field strengths to 25kV/cm when used with BTX generators! Gamma irradiated for guaranteed sterility.

Gap Size Volume Catalog No. Pack of 501mm † 70µL B013-FB101 $110.002mm † 400µL B013-FB102 $110.004mm † 800µL B013-FB104 $110.00

Fisher Ordering InformationFisherBiotech Cuvets: Quality Plus Economy

† Does not include micropipet.


DNA PURIFICATION

Montage Genomics Kits

Automation-Certified Millex filters are specif-ically designed for automated filtrationinstruments for dissolution testing anddosage form analysis—with proven perfor-mance on the Zymark BenchMate, Prelude,TPWII, and MultiDose, and MultiDose Plusworkstations. Available in packs of 200 (8 tubes of 25). ‡

Automation-Certified Millex Filter Catalog No. PriceWith PVDF 0.45µm filter B013-SLHV DZ5 NZ $361.00With PVDF 0.45µm filter, Glass Fiber B prefilter B013-SLHV BZ5 NZ $412.00With Hydrophilic PTFE 0.45µm filter B013-SLCR DZ5 NZ $361.00With Hydrophilic PTFE 0.45µm filter, Glass Fiber B prefilter B013-SLCR BZ5 NZ $412.00

‡ 1,000 bulk pack is also available. For ordering information, call your Fisher Representative or visitwww.millipore.com/fisher.

Description Quantity † Catalog No. PricePCR96 Cleanup Kit 4 B013-LSKC 096 04 $400.00

24 B013-LSKC 096 24 $1,675.00Plasmid Miniprep96 Kit 4 B013-LSKP 096 04 $425.00

24 B013-LSKP 096 24 $2,200.00

Minimal hands-on timeUnlike bind-and-elute protocols, the Montagekits allow samples to be collected from thetop of the plate—providing easy pipetteraccess. And since the protocols have no centrifugation steps, these kits are ideal for use with a range of automated liquid-handling systems.

Validated protocols and softwareValidated protocols and software programsare currently available for the systems listedbelow. Below is a partial list of the auto-mated equipment currently used withMillipore kits. For details, contact your Fisher Sales Representative or visit www.millipore.com/fisher.

Automated PCR CleanupMontage PCR96 Cleanup Kits remove unincor-porated primers and other contaminants toobtain PCR products with 98% purity (typical).Recovery is typically >90% for 500bp frag-ments or longer.

Automated Plasmid MiniprepsThe Montage Plasmid Miniprep96 Kit provides a reproducible, high-recoverymeans of purifying plasmid DNA with yields typically around 4 to 6µg from 1mLof bacterial culture.

Additional Automation-Compatible Products

† Number of 96-well plates or preps.

96-well purification plates...designed with automation in mind.



©2001 Fisher ScientificLitho in U.S.A.

01-1727JT/RS 15M-SW-07/ 01

BN0626015

Fisher ScientificFor customer service, call 1-800-766-7000. To fax an order, use 1-800-926-1166.To order online: www.fishersci.com

Automation-Ready—from Millipore!

Field Automation SupportOur Field Automation Specialists are avail-able to assist you in implementing Milliporeproducts and protocols—including ourMontage PCR Cleanup and Plasmid Miniprepkits—onto your automated liquid-handlingsystems. Contact your Fisher SalesRepresentative for details.

PCR • Beckman MultimekCleanup • Beckman Biomek 2000

• TomTec Quadra 96Plasmid • Beckman Biomek 2000Minipreps • Packard

MultiPROBE II EX

High-Quality, High-Recovery Separations


Documents

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