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Hershey's work on T2 phage. DNA, not protein is hereditary substance. CONTENT. 1.Hershey History. 2. What is T2??. 3. Infective Cycle. 4. Hershey Chase Experiment. 5. Conclusion. Alfred Day Hershey. His best known experiment is Hershey Chase experiment - PowerPoint PPT Presentation
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Alfred Day Hershey His best known
experiment is Hershey Chase experiment
Concerned the replication of genetic structure of viruses
4 Dec. 1908--- 22 May 1997
1934 -1950 Teaching and research at the Department of Bacteriology1936 Held an instructorship in bacteriology and immunology 1936 - 1939 Work together with Professor J. J. Bronfenbrenner. 1938 Became assistant professor 1942 Became associate professor
What is T2 ?What is T2 ?•The T2 phage is a strongly toxic bacteriophage and tadpole-shaped virus
• small virus that infect only bacteria
•Contain 50% DNA and 50% protein
•Contains linear double-stranded DNA
Infect E-coli
tail fibers of the phage attach to the bacterial cell to initiate infection
Protein coat of the T2 carries the organ of attachment to bacteria.
•Virus attach to the surface of their host cellVirus attach to the surface of their host cell (a)
The infective cycle of T2 in E.coil
•Inject the DNA into the cellInject the DNA into the cell (b)
•DNA accumulate,other genes are transcribed and translatedDNA accumulate,other genes are transcribed and translated (C)
•DNA and protein are assembled into complete virusDNA and protein are assembled into complete virus (d)
•The cell breaks and releases its content of virusThe cell breaks and releases its content of virus (e)
Hershey Chase Experiment Radioactive
Sulphur (35S)
non-radioative DNA?
Radioactive Phosphorous(35P)
radioactive DNA?