Hepatoprotective & synergistic efficacy of polyherbal formulation from.pptx

8/9/2019 Hepatoprotective & synergistic efficacy of polyherbal formulation from.pptx

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HEPATOPROTECTIVE & SYNERGISTICEFFICACY OF POLYHERBAL

FORMULATION FROM TINOSPORACORDIFOLIA AND MURRAYA KOENIGII LAGAINST CARBON TETRACHLORIDE-INDUCED (CCL 4) TOXICITY: IN VIVO STUDY

V.NISHEETHA (1 F!1S"11#)UNDER THE GUIDANCE OF G.KIRANMAI(ASSISTANT PROFESSOR OFPHARMACOLGY)SS! COLLEGE OF PHARMACY.


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INTRODUCTION

Liver is a vital organ has a wide range offunctions in the body including carbohydrate,

protein and fat metabolism, detoxification,secretion of bile and storage of vitamins,synthesis of plasma protein and glycogenstorage .

The liver is also prone to many diseases mostlyincluded are infectious such as hepatitis A,B,C,E,alcohol damage, fatty liver, cirrhosis, cancer,drug damage especially acetaminophen, cancerdrugs .


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Carbon tetrachloride CCl ! " induced hepatotoxicity modelis widely used model for the study of hepatoprotective

effects of drugs and plant extracts .

#atural remedies from medicinal plants are considered to be effective and safe alternative treatment for liverdiseases. Therefore, many fol$ remedies from plant originare tested for its potential antioxidant andhepatoprotective liver damage in experimental animalmodel.

%olyherbal formulation from Tinospora cordifolia and&urraya $oenigii L against Carbon Tetrachloride'

(nduced ccl ! " toxicity, is studied .


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TINOSPORA CORDIFOLIA

CLASSIFICATION KINGDOM- PLANTAE

DIVISION: MANGOLIOPHYTA,

CLASS: MAGNOLIOPSIDA,

ORDER: RANUNCULALES,

FAMILY: MENISPERMACEAE,

GENUS: TINOSPORA,

SPECIES: T. CORDIFOLIA,

BOTANICAL NAME: TINOSPORA CORDIFOLIA

HERBAL PRODUCTS


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CLASSIFICATION

KINGDOM: PLANTAE

DIVISION: ROSIDS

CLASS: EUDICOTS

ORDER: SAPINDALES

FAMILY: RUTACEAE

GENUS: MURRAYA

SPECIES: MURRAYA KOENIGII

BOTANICAL NAME: MURRAYA KOENIGII

MURRAYA KOENIGII


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0. Biochemical and histopathological studies

$. Assesment of lipid peroxidation.

)1. 2erum ALT, A2T and Total Bilirubin levels.

)). 3istopathology .


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%reparation of plant extractsThe leaves of Tinospo ! "o #i$o%i! and

M& !'! Ko*ni)ii &4" were collected

from local mar$et.

Leaves were shade dried and pulveri5ed to a

coarse powder and extracted with ethanol. The

filtrate obtained was evaporated to dryness at1'- 6C in a rotary vacuum evaporator to

obtain a dar$ colored molten mass.


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%hytochemical analysis 7

2creening is done to find the presence of the

active ingredients in the leaf extracts with

different solvents.

Antimicrobial activity 7Antimicrobial activity indicates that biological activity of

crude extracts, (n that we are both 8ram negative, 8ram

positive bacteria for determination of &(C &inimum

(nhibitory Concentration", &BC and antifungal activity of

the extracts will be estimated.


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Experimental design.G% ' 1: #ormal control

G% ' : CCl ! control group

G% ' : 2tandard drug sylimarin +1mg@$g body

weight CCl !

G% ' - ! 7 TC') 1mg@$g CCl !

G% ' - 7 &&4 ) 1mg@$g CCl !

G% ' *: +11 mg +,% / 0 % '/ 23 @$g body

weight ccl !


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PROCEDUREAnimal groups were divided and treated

accordingly.) st group ;Treated normally with orally 1. ofC&C for seven days, and then intraperitoniallyin ected with )1 ml @ $g body weight olive oil.

*nd group ' served as CCl! hepatotoxicity control

and was orally given 1. C&C for seven daysand then intrateritoneally intoxicated with ccl!)1ml@$g body weight 1.) in olive oil".


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Third group served as standard group and

received reference drug, silymarian * mg @ $g

@day, p.o" for seven days prior to CCl!

intoxication .

=ourth group of animals were pretreated with ) 1

mg @ $g body weight of plant extract during the

seven days, and then intoxicated with CCl!

)1ml@$g body weight 1.) in olive oil".


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All animals were sacrificed under mild ether anesthesia

)- hours after ccl! in ection, and serum was separated at

* 11 rpm for ) min and biochemical investigations

were carried out.

Liver was dissected out and used for biochemicaldeterminations and liver tissue slices were collected for

histopathological studies and blood samples werecollected immediately.


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DAT(


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S,%' ALT5 AST 6 T 2 / B3/3%' 3 /,7,/8There was a significant increase in serum en5ymes ALT

and A2T activity of CCl! treated groups whencompared with that of normal control.

H382 2+ / 9Animals were sacrificed to remove liver. The liver wasfixed in BouinFs solution for)* hr, and then embedded in

paraffin using conventional methods 8alighor )G/-" ,cut into +Hm thic$ sections and stained usinghaematoxylinbosin dye. The sections were thenobserved for histopathological changes.


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C ;/'83 :3ence the comparison is made between the

groups of animals divided and observed for the

serum levels and histopatology results and animal

is sacrificed.


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R,0,%, ;,8" &urraya $oenigii information from #%82@8>(#I .www.ars'grin.gov. >etrieved *110'1+')). !' ' < Arulselvan %, 2enthil$umar 8%, 2athish 4umar ,2ubramanian 2 =acility for>asayana
http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://en.wikipedia.org/wiki/Curry_treehttp://en.wikipedia.org/wiki/PubMed_Identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17069429http://en.wikipedia.org/wiki/Tinospora_cordifoliahttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://en.wikipedia.org/wiki/Tinospora_cordifoliahttp://www.frlht.org/rasayana/node/43http://www.frlht.org/rasayana/node/43http://www.frlht.org/rasayana/node/43http://www.frlht.org/rasayana/node/43http://en.wikipedia.org/wiki/Tinospora_cordifoliahttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://www.henriettesherbal.com/eclectic/usdisp/tinospora.htmlhttp://en.wikipedia.org/wiki/Tinospora_cordifoliahttp://www.ncbi.nlm.nih.gov/pubmed/17069429http://en.wikipedia.org/wiki/PubMed_Identifierhttp://en.wikipedia.org/wiki/Curry_treehttp://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?24703


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Hepatoprotective & synergistic efficacy of polyherbal formulation from.pptx